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The map label for this gene is miaA

Identifier: 186477235

GI number: 186477235

Start: 2796051

End: 2797004

Strand: Direct

Name: miaA

Synonym: Bphy_2486

Alternate gene names: 186477235

Gene position: 2796051-2797004 (Clockwise)

Preceding gene: 186477234

Following gene: 186477237

Centisome position: 80.37

GC content: 66.98

Gene sequence:

>954_bases
ATGATGCAGCGCGCACCGCAACCCATCGCCTGTCTTCTCGGCCCGACCGCCTCCGGCAAGACGGCGGCCGCGCTCGCGTT
CGCCGCGCGGGCACCCGTCGAGATCGTCAGCGTCGACTCGGCGCTCGTCTATCGCGAGATGGACATCGGCACTGCGAAGC
CGTCGTCGGAAGAGCGTGCCGTCGCGCCTCACCATCTGATCGACATCGTCGATCCCGTGGACGCCTACTCCGCCGCCGAT
TTCCGCGCCGACGCGCTGCGCCTCGTCGGCGAGATCGAGGCGCGCGGCAACGTGCCGTTGCTGGTCGGCGGCACGATGCT
GTACTACAAGGCGCTGACGCAGGGCCTGAACGATCTGCCCGCGGCCGACCCCGAGGTGCGCGCGACGCTTGACGCCGACG
CCGCACGCGAAGGCTGGCCGGCGCTGCACGCGCGTTTGGCTGCCGTCGATGCGGTTACGGCTGCGCGCCTCGCGCCCAAC
GATTCGCAGCGTATCCAGCGTGCGCTCGAGGTATTCATGCTAACGGGGCAGCCGATGTCGGCGTTGCTTGCTGCGCCAGC
GCGCAGTGATGACCTGGCGAAGGGGTATCGCTTTGTGCCCATTGCGCTGGAGCCGTCCGACCGTAGCGTGCTGCATGCCC
GTATCGCACAGCGGTTCGATGCGATGCTCGCGAAGGGGTTTATCGATGAAGTACGCCGGCTGCGCGCACGCGGCGACCTG
CATCCCGGACTGCCGGCGATGCGCTGCGTCGGGTATCGGCAGGCTTGGGAATATCTCGACGGCGAGACCGATTACGACAC
CATGCGGGATAAGGGCGTCTTTGCGACGCGGCAACTGTGCAAGCGTCAACTCACCTGGCTGCGTGCGATGCCCGAACGTA
TCGTCGTTGATTGCTGCGCAGGGGATGCTGCCGGGCTAGCCTTGCAGGCTATTGAGCGGGTTATTGCCGGGTAG

Upstream 100 bases:

>100_bases
GGGCGCCCGACGTGGTATCAGCTGACGCTCGCCGATCTCGACCGGCTGTTCATGCGCGGTCAGTGAGCGGCTCCGTTTCT
TTCATTCGCTCACGCTTTTC

Downstream 100 bases:

>100_bases
TTTTCCTGTTTGTCTGCGACGCGTGGCGAAAGAAAGCCGCTCACACCGCGAGCCCGTGTTCCTGCCGACGGGCTCTCAAC
GCCCCAGGCCTTCCAGGGCG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MMQRAPQPIACLLGPTASGKTAAALAFAARAPVEIVSVDSALVYREMDIGTAKPSSEERAVAPHHLIDIVDPVDAYSAAD
FRADALRLVGEIEARGNVPLLVGGTMLYYKALTQGLNDLPAADPEVRATLDADAAREGWPALHARLAAVDAVTAARLAPN
DSQRIQRALEVFMLTGQPMSALLAAPARSDDLAKGYRFVPIALEPSDRSVLHARIAQRFDAMLAKGFIDEVRRLRARGDL
HPGLPAMRCVGYRQAWEYLDGETDYDTMRDKGVFATRQLCKRQLTWLRAMPERIVVDCCAGDAAGLALQAIERVIAG

Sequences:

>Translated_317_residues
MMQRAPQPIACLLGPTASGKTAAALAFAARAPVEIVSVDSALVYREMDIGTAKPSSEERAVAPHHLIDIVDPVDAYSAAD
FRADALRLVGEIEARGNVPLLVGGTMLYYKALTQGLNDLPAADPEVRATLDADAAREGWPALHARLAAVDAVTAARLAPN
DSQRIQRALEVFMLTGQPMSALLAAPARSDDLAKGYRFVPIALEPSDRSVLHARIAQRFDAMLAKGFIDEVRRLRARGDL
HPGLPAMRCVGYRQAWEYLDGETDYDTMRDKGVFATRQLCKRQLTWLRAMPERIVVDCCAGDAAGLALQAIERVIAG
>Mature_317_residues
MMQRAPQPIACLLGPTASGKTAAALAFAARAPVEIVSVDSALVYREMDIGTAKPSSEERAVAPHHLIDIVDPVDAYSAAD
FRADALRLVGEIEARGNVPLLVGGTMLYYKALTQGLNDLPAADPEVRATLDADAAREGWPALHARLAAVDAVTAARLAPN
DSQRIQRALEVFMLTGQPMSALLAAPARSDDLAKGYRFVPIALEPSDRSVLHARIAQRFDAMLAKGFIDEVRRLRARGDL
HPGLPAMRCVGYRQAWEYLDGETDYDTMRDKGVFATRQLCKRQLTWLRAMPERIVVDCCAGDAAGLALQAIERVIAG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=32.7974276527331, Blast_Score=137, Evalue=1e-32,
Organism=Escherichia coli, GI1790613, Length=278, Percent_Identity=57.9136690647482, Blast_Score=320, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI25144712, Length=303, Percent_Identity=28.3828382838284, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324848, Length=296, Percent_Identity=25.6756756756757, Blast_Score=122, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24649830, Length=291, Percent_Identity=32.9896907216495, Blast_Score=134, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_BURP8 (B2JGD1)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001858705.1
- ProteinModelPortal:   B2JGD1
- SMR:   B2JGD1
- GeneID:   6243970
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_2486
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34283; Mature: 34283

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQRAPQPIACLLGPTASGKTAAALAFAARAPVEIVSVDSALVYREMDIGTAKPSSEERA
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEECCHHHHEEECCCCCCCCCCCCCC
VAPHHLIDIVDPVDAYSAADFRADALRLVGEIEARGNVPLLVGGTMLYYKALTQGLNDLP
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHCHHCCC
AADPEVRATLDADAAREGWPALHARLAAVDAVTAARLAPNDSQRIQRALEVFMLTGQPMS
CCCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHH
ALLAAPARSDDLAKGYRFVPIALEPSDRSVLHARIAQRFDAMLAKGFIDEVRRLRARGDL
HHHHCCCCCCHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HPGLPAMRCVGYRQAWEYLDGETDYDTMRDKGVFATRQLCKRQLTWLRAMPERIVVDCCA
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHEEECCC
GDAAGLALQAIERVIAG
CCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMQRAPQPIACLLGPTASGKTAAALAFAARAPVEIVSVDSALVYREMDIGTAKPSSEERA
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEECCHHHHEEECCCCCCCCCCCCCC
VAPHHLIDIVDPVDAYSAADFRADALRLVGEIEARGNVPLLVGGTMLYYKALTQGLNDLP
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHCHHCCC
AADPEVRATLDADAAREGWPALHARLAAVDAVTAARLAPNDSQRIQRALEVFMLTGQPMS
CCCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHH
ALLAAPARSDDLAKGYRFVPIALEPSDRSVLHARIAQRFDAMLAKGFIDEVRRLRARGDL
HHHHCCCCCCHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HPGLPAMRCVGYRQAWEYLDGETDYDTMRDKGVFATRQLCKRQLTWLRAMPERIVVDCCA
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHEEECCC
GDAAGLALQAIERVIAG
CCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA