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The map label for this gene is purR [H]

Identifier: 186477105

GI number: 186477105

Start: 2641531

End: 2642565

Strand: Direct

Name: purR [H]

Synonym: Bphy_2354

Alternate gene names: 186477105

Gene position: 2641531-2642565 (Clockwise)

Preceding gene: 186477099

Following gene: 186477106

Centisome position: 75.92

GC content: 66.38

Gene sequence:

>1035_bases
ATGTCCACCTTGAGCGAAGTCGCGCGGCGCGCCGGCGTCACGTCGGCCACCGTTTCGAACGTGCTGCGCAATCGCGGACG
CGTTGGCGAACAGACGCGTCAGCGCGTGCTCGAAGCCGTCGAGGCGCTCAACTATCGTCCGCACCTCGCCGCCCGCGCGC
TCGCCGAAGGCCGCGCGCCGACCATCGCGCTGATGGTGTCGAGCATCGCGAATCCGTTCTATCCCGAGTTCGCGCTCGCC
GCCGAACGCGCGGCGCGCACGAGCGGGCACTTCGTCATCATCTGCAACACGAACGAAGATCCGCACGCAGGCCGCACCTA
TCTCGATCAACTGGCGGGCTCGCTTTCCGAAGGTGTGCTCGTGATGAATGCCAATCTCGACTTCGACGATCTGCGCAAGG
CCGAGGCACGCGGGACGCCCGTGGTGCTGTGCATGTGGGAACGTCCTGACGATCCGCCCGGCTTGCCGTGTGTCGCCGTC
GACTTCCGGCTCGCCGGGCGACTTGCCGCCCAGCATCTGATCGAGCTGGGACACAGGCGGATCGGCGCGGTGGTCGGCAG
CAAACCGTCGGGCATCCACGCATCGCGTTACGAAGGTTTCGTCGATGCAATGCGCGAAGCGGGTCTGAAGGTGCTCACGA
AAAACGTGCGCTACGCGCCCGATACGATCGAAGGCGGCTATACGGCGGCGCGCGATCTGCTGGAACGCGACGCGAAGCTC
ACAGCGATCTTTGCGACCAACGACCTGCCCGCGCTCGGCACGATTCACGCCGCCGCCGACCTGGGGCGCGACGTGCCGCA
CGATCTTTCCGTGGTCGGCATCACCGACATTCAGCTCGCGCGGCAATCGCGCCCGCCTCTCACGACAGTCGCTGTGCCAA
CCGTCGAAGCCGCCGAACTCGCCGTCTCGCTGCTGCGCGAGCTGATCGAACGGCCCGCGCTTGCGCACGGCTCGTATCAC
AAGACACCGCGCATCTGCGTGACCTCCGAGCCGAAACTGATCGTGCGTGGCTCGACGCTGAATTACCCGCGCTAG

Upstream 100 bases:

>100_bases
CTCCGATAATCGTTCTGTCGTTTTGCGCATGGTGCCCCGCCTTCGTGGTCAATGCAAATGATGAACGCCTGTCCCATATA
TTGAGGATACGCTGCGAACC

Downstream 100 bases:

>100_bases
ACACCCTCGACGCGACGGCTTCGATACGTCGCATGAATGCGCCGAAGCAGCGCTGTATCGTTCAATCCTGGTCAAAACCG
GTACAAGTGGCTGTTCGACG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSTLSEVARRAGVTSATVSNVLRNRGRVGEQTRQRVLEAVEALNYRPHLAARALAEGRAPTIALMVSSIANPFYPEFALA
AERAARTSGHFVIICNTNEDPHAGRTYLDQLAGSLSEGVLVMNANLDFDDLRKAEARGTPVVLCMWERPDDPPGLPCVAV
DFRLAGRLAAQHLIELGHRRIGAVVGSKPSGIHASRYEGFVDAMREAGLKVLTKNVRYAPDTIEGGYTAARDLLERDAKL
TAIFATNDLPALGTIHAAADLGRDVPHDLSVVGITDIQLARQSRPPLTTVAVPTVEAAELAVSLLRELIERPALAHGSYH
KTPRICVTSEPKLIVRGSTLNYPR

Sequences:

>Translated_344_residues
MSTLSEVARRAGVTSATVSNVLRNRGRVGEQTRQRVLEAVEALNYRPHLAARALAEGRAPTIALMVSSIANPFYPEFALA
AERAARTSGHFVIICNTNEDPHAGRTYLDQLAGSLSEGVLVMNANLDFDDLRKAEARGTPVVLCMWERPDDPPGLPCVAV
DFRLAGRLAAQHLIELGHRRIGAVVGSKPSGIHASRYEGFVDAMREAGLKVLTKNVRYAPDTIEGGYTAARDLLERDAKL
TAIFATNDLPALGTIHAAADLGRDVPHDLSVVGITDIQLARQSRPPLTTVAVPTVEAAELAVSLLRELIERPALAHGSYH
KTPRICVTSEPKLIVRGSTLNYPR
>Mature_343_residues
STLSEVARRAGVTSATVSNVLRNRGRVGEQTRQRVLEAVEALNYRPHLAARALAEGRAPTIALMVSSIANPFYPEFALAA
ERAARTSGHFVIICNTNEDPHAGRTYLDQLAGSLSEGVLVMNANLDFDDLRKAEARGTPVVLCMWERPDDPPGLPCVAVD
FRLAGRLAAQHLIELGHRRIGAVVGSKPSGIHASRYEGFVDAMREAGLKVLTKNVRYAPDTIEGGYTAARDLLERDAKLT
AIFATNDLPALGTIHAAADLGRDVPHDLSVVGITDIQLARQSRPPLTTVAVPTVEAAELAVSLLRELIERPALAHGSYHK
TPRICVTSEPKLIVRGSTLNYPR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=342, Percent_Identity=30.9941520467836, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1790194, Length=324, Percent_Identity=32.4074074074074, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789202, Length=300, Percent_Identity=33.3333333333333, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1787948, Length=310, Percent_Identity=31.6129032258064, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1788474, Length=340, Percent_Identity=32.3529411764706, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789068, Length=315, Percent_Identity=30.4761904761905, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1790715, Length=319, Percent_Identity=25.705329153605, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1787906, Length=328, Percent_Identity=26.5243902439024, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=26.791277258567, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994940, Length=294, Percent_Identity=25.8503401360544, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1786540, Length=296, Percent_Identity=29.3918918918919, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1789456, Length=316, Percent_Identity=27.2151898734177, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37137; Mature: 37006

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLSEVARRAGVTSATVSNVLRNRGRVGEQTRQRVLEAVEALNYRPHLAARALAEGRAP
CCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
TIALMVSSIANPFYPEFALAAERAARTSGHFVIICNTNEDPHAGRTYLDQLAGSLSEGVL
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCEE
VMNANLDFDDLRKAEARGTPVVLCMWERPDDPPGLPCVAVDFRLAGRLAAQHLIELGHRR
EEECCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
IGAVVGSKPSGIHASRYEGFVDAMREAGLKVLTKNVRYAPDTIEGGYTAARDLLERDAKL
HHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEE
TAIFATNDLPALGTIHAAADLGRDVPHDLSVVGITDIQLARQSRPPLTTVAVPTVEAAEL
EEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHCCCCCEEEEEECCHHHHHH
AVSLLRELIERPALAHGSYHKTPRICVTSEPKLIVRGSTLNYPR
HHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCC
>Mature Secondary Structure 
STLSEVARRAGVTSATVSNVLRNRGRVGEQTRQRVLEAVEALNYRPHLAARALAEGRAP
CHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
TIALMVSSIANPFYPEFALAAERAARTSGHFVIICNTNEDPHAGRTYLDQLAGSLSEGVL
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCEE
VMNANLDFDDLRKAEARGTPVVLCMWERPDDPPGLPCVAVDFRLAGRLAAQHLIELGHRR
EEECCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
IGAVVGSKPSGIHASRYEGFVDAMREAGLKVLTKNVRYAPDTIEGGYTAARDLLERDAKL
HHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEE
TAIFATNDLPALGTIHAAADLGRDVPHDLSVVGITDIQLARQSRPPLTTVAVPTVEAAEL
EEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHCCCCCEEEEEECCHHHHHH
AVSLLRELIERPALAHGSYHKTPRICVTSEPKLIVRGSTLNYPR
HHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA