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The map label for this gene is boxB [H]

Identifier: 186476297

GI number: 186476297

Start: 1738770

End: 1740197

Strand: Reverse

Name: boxB [H]

Synonym: Bphy_1539

Alternate gene names: 186476297

Gene position: 1740197-1738770 (Counterclockwise)

Preceding gene: 186476298

Following gene: 186476296

Centisome position: 50.02

GC content: 61.2

Gene sequence:

>1428_bases
ATGTCCACCATCGACTACAGCGACAAGATTCCGAACAACGTCAACCTCGCCGACGACCGCACGCTGCAACGCGCGCTCGA
ACAATGGCAACCGAATTTCCTGTCGTGGTGGGGTGACATGGGCCCCGACGGCTCGCACAACTACGACGTGTATCTGCGCA
CGGCAACGAGCGTCGACCCCGGCGGTTGGGCGCATTTCGACTACGTGAAGATGCCCGATTATCGCTGGGGCATCTTTCTC
GCGCCGGGCGACCCGGAACGCAAGATCAACTTCGGCGAGCATAAGGGCGAAGCGGCCTGGCAGGACGTGCCAGGAGAGCA
TCGCGCGAATCTGCGTCGCATCATCGTCACCCAGGGCGACACCGAGCCGGCTTCCGTCGAACAGCAGCGTCATCTGGGAC
TGACCGCGCCGTCGATGTACGACCTGCGCAACCTGTTCCAGGTGAATGTCGAAGAAGGGCGGCACCTGTGGGCGATGGTG
TATCTGCTGCATCGTTACTTCGGCCGCGACGGGCGCGAAGAAGCGCAAGCATTGCTAGGGCGCCGGTCGGGCGACGACGA
CAATCCGCGCATTCTCGGCGCGTTCAACGAAAAAACGCCTGACTGGCTCGCGTTCTACATGTTCACGTACTTCACCGATC
GCGACGGCAAGTTTCAGCTGAGCGCGCTTGCCGAGTCCGGTTTCGACCCGCTCGCGCGCACGACGAAGTTCATGCTGACG
GAAGAAGCGCATCATATGTTCGTCGGCGAGTCCGGTGTGTCGCGTGTCGTTCAGCGCACCGCGCAAGTGATGAACGAACT
TGGAACGGACGACGTGAACAGGATTCGCGCGGCTGGCGTGATCGATCTGCCGACCATCCAGCGCTATCTGAACTTCCACT
ATTCGGTCACGATCGACCTGTTCGGCGCGGATCAGTCGTCGAATGCGGCGACGTTTTACAACTCGGGTCTCAAGGGCCGC
TACGAAGAAACGAAGCGCGACGACGACCATCAGTTGAACGGGCAAAGCTACAAGCTGCTCGATGTCGAGGACGGCAAGCT
CGTCGAACGCGAAGTGCCCATGCTCAATGCGATGAACGAAGTGCTGCGCGACGACTACATCAAGGATTCCGTTGCTGGCG
TCGGACGCTGGAACAAGGTACTGGAGAAGGCGGGTATCGATTGTCGTCTGACCGTGCCGCACAAGGCGTTCAACCGTCAG
ATCGGCACTTTCGCGGGCACTCGTGTGTCGCCCGATGGACGTGTCATTGGCGAAGCAGAGTGGGCCGCGAACGAAGCGAA
CTGGCTGCCGGGCCCGGAAGACCGCGCTTACGTCGCATCGCTGATGGGCCGCGTCGTCGAAGCCGGCCGGTTCGCGAACT
GGATCGCGCCGCCCGCGATGGGCATCAACCGTCAGCCCGTCGATTTCGAATACGTGCGCTTCAACTGA

Upstream 100 bases:

>100_bases
GTGTACGGCAAGGGCAGCAAGGCGCAATTCGACGTCTCGCGTGTCTGACCCACGCACGCGCATCTACATCGTGAACCTGC
ATCGCGCTCAAGGAACCGAC

Downstream 100 bases:

>100_bases
GCATCGCGCGCGACGGCGAGGAAAAGCAGAGGCAGGGAGACATTCATGAACGCGCCTGTATCGATCGAAGTCCTCAAGCA
GCATCTGATCGATCCGGAGA

Product: benzoyl-CoA oxygenase subunit BoxB

Products: NA

Alternate protein names: Benzoyl-CoA 2,3-dioxygenase subunit B; Benzoyl-CoA dioxygenase oxygenase component [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSTIDYSDKIPNNVNLADDRTLQRALEQWQPNFLSWWGDMGPDGSHNYDVYLRTATSVDPGGWAHFDYVKMPDYRWGIFL
APGDPERKINFGEHKGEAAWQDVPGEHRANLRRIIVTQGDTEPASVEQQRHLGLTAPSMYDLRNLFQVNVEEGRHLWAMV
YLLHRYFGRDGREEAQALLGRRSGDDDNPRILGAFNEKTPDWLAFYMFTYFTDRDGKFQLSALAESGFDPLARTTKFMLT
EEAHHMFVGESGVSRVVQRTAQVMNELGTDDVNRIRAAGVIDLPTIQRYLNFHYSVTIDLFGADQSSNAATFYNSGLKGR
YEETKRDDDHQLNGQSYKLLDVEDGKLVEREVPMLNAMNEVLRDDYIKDSVAGVGRWNKVLEKAGIDCRLTVPHKAFNRQ
IGTFAGTRVSPDGRVIGEAEWAANEANWLPGPEDRAYVASLMGRVVEAGRFANWIAPPAMGINRQPVDFEYVRFN

Sequences:

>Translated_475_residues
MSTIDYSDKIPNNVNLADDRTLQRALEQWQPNFLSWWGDMGPDGSHNYDVYLRTATSVDPGGWAHFDYVKMPDYRWGIFL
APGDPERKINFGEHKGEAAWQDVPGEHRANLRRIIVTQGDTEPASVEQQRHLGLTAPSMYDLRNLFQVNVEEGRHLWAMV
YLLHRYFGRDGREEAQALLGRRSGDDDNPRILGAFNEKTPDWLAFYMFTYFTDRDGKFQLSALAESGFDPLARTTKFMLT
EEAHHMFVGESGVSRVVQRTAQVMNELGTDDVNRIRAAGVIDLPTIQRYLNFHYSVTIDLFGADQSSNAATFYNSGLKGR
YEETKRDDDHQLNGQSYKLLDVEDGKLVEREVPMLNAMNEVLRDDYIKDSVAGVGRWNKVLEKAGIDCRLTVPHKAFNRQ
IGTFAGTRVSPDGRVIGEAEWAANEANWLPGPEDRAYVASLMGRVVEAGRFANWIAPPAMGINRQPVDFEYVRFN
>Mature_474_residues
STIDYSDKIPNNVNLADDRTLQRALEQWQPNFLSWWGDMGPDGSHNYDVYLRTATSVDPGGWAHFDYVKMPDYRWGIFLA
PGDPERKINFGEHKGEAAWQDVPGEHRANLRRIIVTQGDTEPASVEQQRHLGLTAPSMYDLRNLFQVNVEEGRHLWAMVY
LLHRYFGRDGREEAQALLGRRSGDDDNPRILGAFNEKTPDWLAFYMFTYFTDRDGKFQLSALAESGFDPLARTTKFMLTE
EAHHMFVGESGVSRVVQRTAQVMNELGTDDVNRIRAAGVIDLPTIQRYLNFHYSVTIDLFGADQSSNAATFYNSGLKGRY
EETKRDDDHQLNGQSYKLLDVEDGKLVEREVPMLNAMNEVLRDDYIKDSVAGVGRWNKVLEKAGIDCRLTVPHKAFNRQI
GTFAGTRVSPDGRVIGEAEWAANEANWLPGPEDRAYVASLMGRVVEAGRFANWIAPPAMGINRQPVDFEYVRFN

Specific function: The BoxA/BoxB complex catalyzes the aerobic reduction/oxygenation of the aromatic ring of benzoyl-CoA to form 2,3-dihydro-2,3-dihydroxybenzoyl-CoA. BoxB acts as the benzoyl-CoA oxygenase, after being reduced by the reductase component BoxA. BoxAB does not

COG id: COG3396

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the benzoyl-CoA oxygenase component B family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017635
- InterPro:   IPR012347
- InterPro:   IPR009078
- InterPro:   IPR007814 [H]

Pfam domain/function: PF05138 PaaA_PaaC [H]

EC number: =1.14.12.21 [H]

Molecular weight: Translated: 53985; Mature: 53854

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIDYSDKIPNNVNLADDRTLQRALEQWQPNFLSWWGDMGPDGSHNYDVYLRTATSVDP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEEEEECCCCC
GGWAHFDYVKMPDYRWGIFLAPGDPERKINFGEHKGEAAWQDVPGEHRANLRRIIVTQGD
CCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCHHCCCCCHHHCCEEEEEEECCC
TEPASVEQQRHLGLTAPSMYDLRNLFQVNVEEGRHLWAMVYLLHRYFGRDGREEAQALLG
CCCCHHHHHHHCCCCCCHHHHHHHHHEEEHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHH
RRSGDDDNPRILGAFNEKTPDWLAFYMFTYFTDRDGKFQLSALAESGFDPLARTTKFMLT
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHEECCCCCEEEEEHHHCCCCHHHHHHHHHEE
EEAHHMFVGESGVSRVVQRTAQVMNELGTDDVNRIRAAGVIDLPTIQRYLNFHYSVTIDL
CCCHHEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEHHHHHHHHCCEEEEEEEE
FGADQSSNAATFYNSGLKGRYEETKRDDDHQLNGQSYKLLDVEDGKLVEREVPMLNAMNE
EECCCCCCCEEEECCCCCCCCHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
VLRDDYIKDSVAGVGRWNKVLEKAGIDCRLTVPHKAFNRQIGTFAGTRVSPDGRVIGEAE
HHHHHHHHHHHHHCHHHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCEECCCCCEEECHH
WAANEANWLPGPEDRAYVASLMGRVVEAGRFANWIAPPAMGINRQPVDFEYVRFN
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure 
STIDYSDKIPNNVNLADDRTLQRALEQWQPNFLSWWGDMGPDGSHNYDVYLRTATSVDP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEEEEECCCCC
GGWAHFDYVKMPDYRWGIFLAPGDPERKINFGEHKGEAAWQDVPGEHRANLRRIIVTQGD
CCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCHHCCCCCHHHCCEEEEEEECCC
TEPASVEQQRHLGLTAPSMYDLRNLFQVNVEEGRHLWAMVYLLHRYFGRDGREEAQALLG
CCCCHHHHHHHCCCCCCHHHHHHHHHEEEHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHH
RRSGDDDNPRILGAFNEKTPDWLAFYMFTYFTDRDGKFQLSALAESGFDPLARTTKFMLT
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHEECCCCCEEEEEHHHCCCCHHHHHHHHHEE
EEAHHMFVGESGVSRVVQRTAQVMNELGTDDVNRIRAAGVIDLPTIQRYLNFHYSVTIDL
CCCHHEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEHHHHHHHHCCEEEEEEEE
FGADQSSNAATFYNSGLKGRYEETKRDDDHQLNGQSYKLLDVEDGKLVEREVPMLNAMNE
EECCCCCCCEEEECCCCCCCCHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
VLRDDYIKDSVAGVGRWNKVLEKAGIDCRLTVPHKAFNRQIGTFAGTRVSPDGRVIGEAE
HHHHHHHHHHHHHCHHHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCEECCCCCEEECHH
WAANEANWLPGPEDRAYVASLMGRVVEAGRFANWIAPPAMGINRQPVDFEYVRFN
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11222587; 12399500 [H]