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The map label for this gene is boxA [H]

Identifier: 186476296

GI number: 186476296

Start: 1737474

End: 1738724

Strand: Reverse

Name: boxA [H]

Synonym: Bphy_1538

Alternate gene names: 186476296

Gene position: 1738724-1737474 (Counterclockwise)

Preceding gene: 186476297

Following gene: 186476294

Centisome position: 49.98

GC content: 62.83

Gene sequence:

>1251_bases
ATGAACGCGCCTGTATCGATCGAAGTCCTCAAGCAGCATCTGATCGATCCGGAGATCTGCATTCGCTGCAACACATGCGA
AGAGACCTGCCCGATCGATGCGATCCAGCACGACGACACGAACTACGTCGTGATGCCGGACGTCTGCAACGGTTGCATGG
CGTGCGTGCCGCCGTGTCCGACGGGCGCGATCGACAACTGGCGCACCGTGCTGAAGGCCGACGCATGGAGCGTCGACGAA
CAGTACACGTGGGACGTGTTGCCCGAACAGGGAACAATGGCATTGCCCGCGACAGCCGGGCCCGCGACCGGCGCCATGAC
CGGCGGCAGTGATGGTGACGGTGAAGCACAGGGCATCGAGGTCGACACGGTGCGCGGTGCGGTGGTGCCACCGTGGTCCG
CCGCGCGTCCGTATGTGAATCTCTACACACACAGGAACCCGACGAAGGCGACTGTCGTCGGCAACTACCGGCTGACAGAC
GAAGGGACGGAAAGCGACATACATCACATCGTGCTCGATTTCGGCTCGCTGCCGTTTCCTGTGCTGGAAGGGCAGTCGAT
CGGCATTCTGCCGCCGGGCAGCGCCGCCGATGGCCGCGCCCATCATGCGCGCCAGTATTCGATCGCCAGTCCGCGCGACG
GCGAGCGGCCCGGCTACAACAACGTGTCGCTGACCGTGAAGCGCGTCACGCAGGACTATCGCGGCAATGCGGCGAGCGGC
GTGTGCTCGAACTATCTGTGCGATCTGAAAAAGGGCGACGTAGTCAATGTGATCGGCCCGTTCGGCAGCACGTTCCTGAT
GCCCAATCATCCGGATTCGCATCTGCTGATGATCTGCACGGGCACGGGGTCCGCGCCGATGCGCGCGATGACCGAGTATC
GCCGGCGACGCAGGCTCAAGGGGGCGACGGGCAAACTGATGCTGTTCTTCGGTGCGCGCACGAAAGCAGAGCTGCCGTAC
TTCGGTCCGCTGACGAATCTGCCGAAAGACTTCATCGACACGAATCTGGCGTTTTCGCGCACGCCCGGCGAACGGAAGCG
CTACGTGCAGGACGCCATGCGCGAGCGTGCCGTCGATGTCGCGCAACTGCTTCGCGACGACGGCACGTACATCTACGTGT
GCGGGTTGAAGGGCATGGAAGACGGGGTGCTGCAGTCGCTCAAGGACATTGCCGATCAGCATGGCCTCGAATGGAACGCG
CTATGGCAGAGGCTCAAGCGCGAAGGACGTCTGCATCTGGAAACGTACTGA

Upstream 100 bases:

>100_bases
CGCGATGGGCATCAACCGTCAGCCCGTCGATTTCGAATACGTGCGCTTCAACTGAGCATCGCGCGCGACGGCGAGGAAAA
GCAGAGGCAGGGAGACATTC

Downstream 100 bases:

>100_bases
ACGCGAGCGGGCGTTACGTCTTCGCAGCCGGCGATTTCGCCCCGCACGCGCGAATCACGAGTTGCACGACCTGATCCGTC
GTTGCGTCGAAAGCCTTTTG

Product: benzoyl-CoA oxygenase/reductase, BoxA protein

Products: NA

Alternate protein names: Benzoyl-CoA 2,3-dioxygenase subunit A; Benzoyl-CoA dioxygenase reductase component [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MNAPVSIEVLKQHLIDPEICIRCNTCEETCPIDAIQHDDTNYVVMPDVCNGCMACVPPCPTGAIDNWRTVLKADAWSVDE
QYTWDVLPEQGTMALPATAGPATGAMTGGSDGDGEAQGIEVDTVRGAVVPPWSAARPYVNLYTHRNPTKATVVGNYRLTD
EGTESDIHHIVLDFGSLPFPVLEGQSIGILPPGSAADGRAHHARQYSIASPRDGERPGYNNVSLTVKRVTQDYRGNAASG
VCSNYLCDLKKGDVVNVIGPFGSTFLMPNHPDSHLLMICTGTGSAPMRAMTEYRRRRRLKGATGKLMLFFGARTKAELPY
FGPLTNLPKDFIDTNLAFSRTPGERKRYVQDAMRERAVDVAQLLRDDGTYIYVCGLKGMEDGVLQSLKDIADQHGLEWNA
LWQRLKREGRLHLETY

Sequences:

>Translated_416_residues
MNAPVSIEVLKQHLIDPEICIRCNTCEETCPIDAIQHDDTNYVVMPDVCNGCMACVPPCPTGAIDNWRTVLKADAWSVDE
QYTWDVLPEQGTMALPATAGPATGAMTGGSDGDGEAQGIEVDTVRGAVVPPWSAARPYVNLYTHRNPTKATVVGNYRLTD
EGTESDIHHIVLDFGSLPFPVLEGQSIGILPPGSAADGRAHHARQYSIASPRDGERPGYNNVSLTVKRVTQDYRGNAASG
VCSNYLCDLKKGDVVNVIGPFGSTFLMPNHPDSHLLMICTGTGSAPMRAMTEYRRRRRLKGATGKLMLFFGARTKAELPY
FGPLTNLPKDFIDTNLAFSRTPGERKRYVQDAMRERAVDVAQLLRDDGTYIYVCGLKGMEDGVLQSLKDIADQHGLEWNA
LWQRLKREGRLHLETY
>Mature_416_residues
MNAPVSIEVLKQHLIDPEICIRCNTCEETCPIDAIQHDDTNYVVMPDVCNGCMACVPPCPTGAIDNWRTVLKADAWSVDE
QYTWDVLPEQGTMALPATAGPATGAMTGGSDGDGEAQGIEVDTVRGAVVPPWSAARPYVNLYTHRNPTKATVVGNYRLTD
EGTESDIHHIVLDFGSLPFPVLEGQSIGILPPGSAADGRAHHARQYSIASPRDGERPGYNNVSLTVKRVTQDYRGNAASG
VCSNYLCDLKKGDVVNVIGPFGSTFLMPNHPDSHLLMICTGTGSAPMRAMTEYRRRRRLKGATGKLMLFFGARTKAELPY
FGPLTNLPKDFIDTNLAFSRTPGERKRYVQDAMRERAVDVAQLLRDDGTYIYVCGLKGMEDGVLQSLKDIADQHGLEWNA
LWQRLKREGRLHLETY

Specific function: The BoxA/BoxB complex catalyzes the aerobic reduction/oxygenation of the aromatic ring of benzoyl-CoA to form 2,3-dihydro-2,3-dihydroxybenzoyl-CoA. BoxA acts as a reductase that uses NADPH to reduce the oxygenase component BoxB. BoxAB does not act on NADH

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Homo sapiens, GI24041029, Length=196, Percent_Identity=31.6326530612245, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI10835173, Length=226, Percent_Identity=29.646017699115, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI40254422, Length=199, Percent_Identity=32.6633165829146, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789123, Length=197, Percent_Identity=34.5177664974619, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321143, Length=225, Percent_Identity=31.1111111111111, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6321832, Length=163, Percent_Identity=31.2883435582822, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24660907, Length=157, Percent_Identity=35.031847133758, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24660903, Length=157, Percent_Identity=35.031847133758, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24583543, Length=223, Percent_Identity=30.0448430493274, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI78706876, Length=223, Percent_Identity=30.0448430493274, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI78706872, Length=223, Percent_Identity=30.0448430493274, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017634
- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012146
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00037 Fer4; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.21 [H]

Molecular weight: Translated: 45698; Mature: 45698

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPVSIEVLKQHLIDPEICIRCNTCEETCPIDAIQHDDTNYVVMPDVCNGCMACVPPCP
CCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEECCCCEEECCCHHCCCEECCCCCC
TGAIDNWRTVLKADAWSVDEQYTWDVLPEQGTMALPATAGPATGAMTGGSDGDGEAQGIE
CCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEE
VDTVRGAVVPPWSAARPYVNLYTHRNPTKATVVGNYRLTDEGTESDIHHIVLDFGSLPFP
EEECCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEECCCCCCCHHHEEEEECCCCCCC
VLEGQSIGILPPGSAADGRAHHARQYSIASPRDGERPGYNNVSLTVKRVTQDYRGNAASG
EECCCEEEEECCCCCCCCCHHHHHEECCCCCCCCCCCCCCCEEEEEEEHHHHHCCCCHHH
VCSNYLCDLKKGDVVNVIGPFGSTFLMPNHPDSHLLMICTGTGSAPMRAMTEYRRRRRLK
HHHHHHHCCCCCCEEEEECCCCCEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCC
GATGKLMLFFGARTKAELPYFGPLTNLPKDFIDTNLAFSRTPGERKRYVQDAMRERAVDV
CCCCCEEEEECCCCCCCCCCCCCHHCCCHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHH
AQLLRDDGTYIYVCGLKGMEDGVLQSLKDIADQHGLEWNALWQRLKREGRLHLETY
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MNAPVSIEVLKQHLIDPEICIRCNTCEETCPIDAIQHDDTNYVVMPDVCNGCMACVPPCP
CCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEECCCCEEECCCHHCCCEECCCCCC
TGAIDNWRTVLKADAWSVDEQYTWDVLPEQGTMALPATAGPATGAMTGGSDGDGEAQGIE
CCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEE
VDTVRGAVVPPWSAARPYVNLYTHRNPTKATVVGNYRLTDEGTESDIHHIVLDFGSLPFP
EEECCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEECCCCCCCHHHEEEEECCCCCCC
VLEGQSIGILPPGSAADGRAHHARQYSIASPRDGERPGYNNVSLTVKRVTQDYRGNAASG
EECCCEEEEECCCCCCCCCHHHHHEECCCCCCCCCCCCCCCEEEEEEEHHHHHCCCCHHH
VCSNYLCDLKKGDVVNVIGPFGSTFLMPNHPDSHLLMICTGTGSAPMRAMTEYRRRRRLK
HHHHHHHCCCCCCEEEEECCCCCEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCC
GATGKLMLFFGARTKAELPYFGPLTNLPKDFIDTNLAFSRTPGERKRYVQDAMRERAVDV
CCCCCEEEEECCCCCCCCCCCCCHHCCCHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHH
AQLLRDDGTYIYVCGLKGMEDGVLQSLKDIADQHGLEWNALWQRLKREGRLHLETY
HHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11222587; 12399500 [H]