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The map label for this gene is nifU [H]

Identifier: 186476215

GI number: 186476215

Start: 1639365

End: 1639772

Strand: Reverse

Name: nifU [H]

Synonym: Bphy_1457

Alternate gene names: 186476215

Gene position: 1639772-1639365 (Counterclockwise)

Preceding gene: 186476216

Following gene: 186476214

Centisome position: 47.13

GC content: 61.27

Gene sequence:

>408_bases
ATGGCATACAGCGATAAGGTCCTGGACCACTACGAAAACCCGCGCAACGTCGGTTCGTTCGCGAAAGACGACGACACGGT
CGGCACCGGCATGGTCGGCGCACCGGCTTGCGGCGACGTGATGAAGCTGCAGATCCGCGTCGGTGCGGACGGCGTGATTG
AAGACGCGAAGTTCAAGACGTACGGATGCGGTTCGGCCATCGCGTCGAGCTCGCTCGTGACGGAATGGGTGAAGGGCAAG
ACGCTCGACGAGGCGCTGTCGATCAAGAACACGCAGATTGCCGAAGAACTCGCGCTGCCGCCTGTGAAGATTCACTGCTC
GATCCTGGCGGAAGATGCCATCAAGGCAGCCGTCGCCGATTACAAGCAGCGTCACGGTGAAACGGCGAAGGCCGGGCAGT
CAGCGTAA

Upstream 100 bases:

>100_bases
ACGGCATCGATCTGTCGACCATCCAGTGGGCTGCGCACTGAGCGCGCGTCGCGGCGGGTCGAACACGAACGCAGGTTGAA
TCGAATCAAGGAGTGACAAC

Downstream 100 bases:

>100_bases
GCGACGCGTGACGAACGCGCCGGAGTGGCACGGCGCGGCGCTGGATTCGAGTACTTGAATTCGACGCCCGCCGGAACGAT
TTGGGAAGCAGGCAGGGTCG

Product: FeS cluster assembly scaffold IscU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 135; Mature: 134

Protein sequence:

>135_residues
MAYSDKVLDHYENPRNVGSFAKDDDTVGTGMVGAPACGDVMKLQIRVGADGVIEDAKFKTYGCGSAIASSSLVTEWVKGK
TLDEALSIKNTQIAEELALPPVKIHCSILAEDAIKAAVADYKQRHGETAKAGQSA

Sequences:

>Translated_135_residues
MAYSDKVLDHYENPRNVGSFAKDDDTVGTGMVGAPACGDVMKLQIRVGADGVIEDAKFKTYGCGSAIASSSLVTEWVKGK
TLDEALSIKNTQIAEELALPPVKIHCSILAEDAIKAAVADYKQRHGETAKAGQSA
>Mature_134_residues
AYSDKVLDHYENPRNVGSFAKDDDTVGTGMVGAPACGDVMKLQIRVGADGVIEDAKFKTYGCGSAIASSSLVTEWVKGKT
LDEALSIKNTQIAEELALPPVKIHCSILAEDAIKAAVADYKQRHGETAKAGQSA

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=123, Percent_Identity=74.7967479674797, Blast_Score=196, Evalue=4e-51,
Organism=Homo sapiens, GI24307953, Length=123, Percent_Identity=73.9837398373984, Blast_Score=193, Evalue=4e-50,
Organism=Escherichia coli, GI1788878, Length=125, Percent_Identity=81.6, Blast_Score=220, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17543474, Length=127, Percent_Identity=68.503937007874, Blast_Score=189, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6325122, Length=125, Percent_Identity=68, Blast_Score=181, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6324800, Length=127, Percent_Identity=66.9291338582677, Blast_Score=180, Evalue=5e-47,
Organism=Drosophila melanogaster, GI21355597, Length=123, Percent_Identity=73.9837398373984, Blast_Score=194, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011339
- InterPro:   IPR002871 [H]

Pfam domain/function: PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 14242; Mature: 14111

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSDKVLDHYENPRNVGSFAKDDDTVGTGMVGAPACGDVMKLQIRVGADGVIEDAKFKT
CCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCC
YGCGSAIASSSLVTEWVKGKTLDEALSIKNTQIAEELALPPVKIHCSILAEDAIKAAVAD
CCCCHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHHH
YKQRHGETAKAGQSA
HHHHCCCCCCCCCCC
>Mature Secondary Structure 
AYSDKVLDHYENPRNVGSFAKDDDTVGTGMVGAPACGDVMKLQIRVGADGVIEDAKFKT
CCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCC
YGCGSAIASSSLVTEWVKGKTLDEALSIKNTQIAEELALPPVKIHCSILAEDAIKAAVAD
CCCCHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHHH
YKQRHGETAKAGQSA
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]