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The map label for this gene is iscA [H]

Identifier: 186476214

GI number: 186476214

Start: 1638894

End: 1639250

Strand: Reverse

Name: iscA [H]

Synonym: Bphy_1456

Alternate gene names: 186476214

Gene position: 1639250-1638894 (Counterclockwise)

Preceding gene: 186476215

Following gene: 186476213

Centisome position: 47.12

GC content: 58.82

Gene sequence:

>357_bases
ATGAACGGGGCGTCGCGCACATTGAGAAACGCTATGGCAATTACGTTGACCGAAAAGGCAGCACAGCACGTGCAGAAATA
TCTGACCCGCCGCGGCAAGGGCGTGGGGTTGCGGCTCGGTGTGCGCACGACGGGTTGCTCGGGCCTTGCGTACAAGCTCG
AGTATGTCGATGAACTGGCGCCCGAAGACGAGGTCTTCGAGTGCAATGGCGTGAAGATCGTGGTGGACCCGAAGAGCCTC
GCCTATATCGACGGCACGGAGCTCGACTTTGCACGTGAAGGGTTGAACGAAGGCTTCAGGTTCAACAACCCGAACGTGAA
GGACGAATGCGGCTGCGGCGAATCGTTCCGCGTGTAA

Upstream 100 bases:

>100_bases
ACGCGCCGGAGTGGCACGGCGCGGCGCTGGATTCGAGTACTTGAATTCGACGCCCGCCGGAACGATTTGGGAAGCAGGCA
GGGTCGTGCGATGCGTCGTG

Downstream 100 bases:

>100_bases
GCGCGCGAGTCATGCTGAAAAGGCGGCCTGTGCCGCCTTTTTTCAATTTCGCCGCGTATTCAAACGCGCACATTTTCACG
GCGTTGCGCTGCGCGCCCTG

Product: iron-sulfur cluster assembly protein IscA

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 118; Mature: 118

Protein sequence:

>118_residues
MNGASRTLRNAMAITLTEKAAQHVQKYLTRRGKGVGLRLGVRTTGCSGLAYKLEYVDELAPEDEVFECNGVKIVVDPKSL
AYIDGTELDFAREGLNEGFRFNNPNVKDECGCGESFRV

Sequences:

>Translated_118_residues
MNGASRTLRNAMAITLTEKAAQHVQKYLTRRGKGVGLRLGVRTTGCSGLAYKLEYVDELAPEDEVFECNGVKIVVDPKSL
AYIDGTELDFAREGLNEGFRFNNPNVKDECGCGESFRV
>Mature_118_residues
MNGASRTLRNAMAITLTEKAAQHVQKYLTRRGKGVGLRLGVRTTGCSGLAYKLEYVDELAPEDEVFECNGVKIVVDPKSL
AYIDGTELDFAREGLNEGFRFNNPNVKDECGCGESFRV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=41.5094339622642, Blast_Score=97, Evalue=3e-21,
Organism=Homo sapiens, GI160420328, Length=83, Percent_Identity=42.1686746987952, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=70.0934579439252, Blast_Score=161, Evalue=1e-41,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=48.5714285714286, Blast_Score=114, Evalue=3e-27,
Organism=Escherichia coli, GI1786351, Length=111, Percent_Identity=38.7387387387387, Blast_Score=72, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25146411, Length=118, Percent_Identity=37.2881355932203, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=39.622641509434, Blast_Score=95, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71998452, Length=106, Percent_Identity=35.8490566037736, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=46.6666666666667, Blast_Score=97, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24642263, Length=104, Percent_Identity=44.2307692307692, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 12992; Mature: 12992

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGASRTLRNAMAITLTEKAAQHVQKYLTRRGKGVGLRLGVRTTGCSGLAYKLEYVDELA
CCCHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCCEEEEECEECCCCCHHHHHHHHHHHC
PEDEVFECNGVKIVVDPKSLAYIDGTELDFAREGLNEGFRFNNPNVKDECGCGESFRV
CCCCEEEECCEEEEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNGASRTLRNAMAITLTEKAAQHVQKYLTRRGKGVGLRLGVRTTGCSGLAYKLEYVDELA
CCCHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCCEEEEECEECCCCCHHHHHHHHHHHC
PEDEVFECNGVKIVVDPKSLAYIDGTELDFAREGLNEGFRFNNPNVKDECGCGESFRV
CCCCEEEECCEEEEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]