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The map label for this gene is ispG [H]

Identifier: 186476178

GI number: 186476178

Start: 1600006

End: 1601319

Strand: Reverse

Name: ispG [H]

Synonym: Bphy_1420

Alternate gene names: 186476178

Gene position: 1601319-1600006 (Counterclockwise)

Preceding gene: 186476179

Following gene: 186476177

Centisome position: 46.03

GC content: 64.84

Gene sequence:

>1314_bases
ATGCACTCCGATCAAGTGAAATCCAGCAGTCAGATTGTTTCGACCGTGCCGGTGTTCGGCGGTCATGCGCCGCGTCGCCG
CTCGAATGCGGTCGACGTGCGTTGGGGCGGCCAACTCGTGACGATCGGCGGCGACGCGCCCGTGCGCGTGCAGTCGATGA
CCAACACGGATACGGCCGATGCAATCGGCACCGCGATACAGATCAAGGAACTCGCGCAGGCCGGTTCGGAGCTCGTGCGT
ATCACGGTGAATACGCCGGAAGCGGCGGCCGCCGTGCCCGCGATCCGCGAGCAGCTCGACCGCATGGGCGTGACGGTGCC
GCTGGTCGGTGACTTCCATTACAACGGACATTTGTTGCTGCGCGACTATCCGGGCTGCGCGGAATCGCTGTCGAAGTACC
GCATCAACCCGGGCAACGTCGGTCAGGGCGCGAAGCGCGACACGCAGTTCGCGCAGATGATCGAAGCCGCAGCCAGGTAC
GACAAGCCGGTGCGTATCGGCGTGAACTGGGGCAGCCTCGATCAGGACCTGCTCGCGAAGATGATGGACGAGAACGCCGC
GCGCGCCGAACCGTGGGAAGCGCAAAGCGTGATGTACGAAGCGCTGATCCAGTCGGCGATCGGCTCGGCGGAACGTGCTG
TCGAACTGGGTCTCGGCCGCGACAAGATCATCCTGTCGTGCAAGGTGAGCGGCGTGCAGGATCTGATCGCCGTCTACCAG
GAACTCGCGCGCCGCTGCGACTTCGCGCTGCACCTGGGCCTTACGGAAGCGGGCATGGGGTCGAAGGGCATCGTCGCGTC
GACGGCTGCACTGTCCGTGCTGCTGCAGCAGGGCATCGGCGATACGATCCGTATTTCGCTGACGCCGGAACCGGGCGCAT
CGCGCACTGGCGAGGTGATCGTCGGCCAGGAAATCCTGCAGACGATGGGCCTGCGTTCGTTCGCGCCAATGGTCATCGCG
TGCCCGGGTTGCGGGCGCACGACGAGCACGCTGTTCCAGGAGCTGGCGTCGCAGATTCAGAGTTATCTGCGCACCCAGAT
GCCCCTGTGGCGCGATACGTATCCGGGGGTCGAGAAGATGCACGTCGCCGTAATGGGCTGCATCGTCAACGGGCCGGGCG
AATCGAAGCACGCGAACATCGGTATCAGCCTGCCCGGCTCGGGTGAGAACCCGGCTGCGCCCGTCTTCATCGACGGCGTG
AAGGTCAAGACGCTGCGCGGCGAAAACATCGCGCAGGAATTCCAGCAGATCGTGAGCGACTACGTCGAGCGCAGCTATGG
GCCCAACGCGGTCCGCGCCGAAGCGACGAACTGA

Upstream 100 bases:

>100_bases
GGCTTCGGGGCTGCGGCGCTTTTTCAATTCATGCGTCGTTTTTTGCAACAGGGCCCGGCGTGTACCGGGTGCTGAGCGGG
TGACGCAAGCGGGTTTATCG

Downstream 100 bases:

>100_bases
TTCGCCCCATCTCCAATCGTCAATCAAAGACAGATGACTGAACAGAAGAAACGGCTTGAAAAGTTGTCCGGCGTGAAGGG
CATGAACGACATCCTTCCGC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MHSDQVKSSSQIVSTVPVFGGHAPRRRSNAVDVRWGGQLVTIGGDAPVRVQSMTNTDTADAIGTAIQIKELAQAGSELVR
ITVNTPEAAAAVPAIREQLDRMGVTVPLVGDFHYNGHLLLRDYPGCAESLSKYRINPGNVGQGAKRDTQFAQMIEAAARY
DKPVRIGVNWGSLDQDLLAKMMDENAARAEPWEAQSVMYEALIQSAIGSAERAVELGLGRDKIILSCKVSGVQDLIAVYQ
ELARRCDFALHLGLTEAGMGSKGIVASTAALSVLLQQGIGDTIRISLTPEPGASRTGEVIVGQEILQTMGLRSFAPMVIA
CPGCGRTTSTLFQELASQIQSYLRTQMPLWRDTYPGVEKMHVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFIDGV
KVKTLRGENIAQEFQQIVSDYVERSYGPNAVRAEATN

Sequences:

>Translated_437_residues
MHSDQVKSSSQIVSTVPVFGGHAPRRRSNAVDVRWGGQLVTIGGDAPVRVQSMTNTDTADAIGTAIQIKELAQAGSELVR
ITVNTPEAAAAVPAIREQLDRMGVTVPLVGDFHYNGHLLLRDYPGCAESLSKYRINPGNVGQGAKRDTQFAQMIEAAARY
DKPVRIGVNWGSLDQDLLAKMMDENAARAEPWEAQSVMYEALIQSAIGSAERAVELGLGRDKIILSCKVSGVQDLIAVYQ
ELARRCDFALHLGLTEAGMGSKGIVASTAALSVLLQQGIGDTIRISLTPEPGASRTGEVIVGQEILQTMGLRSFAPMVIA
CPGCGRTTSTLFQELASQIQSYLRTQMPLWRDTYPGVEKMHVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFIDGV
KVKTLRGENIAQEFQQIVSDYVERSYGPNAVRAEATN
>Mature_437_residues
MHSDQVKSSSQIVSTVPVFGGHAPRRRSNAVDVRWGGQLVTIGGDAPVRVQSMTNTDTADAIGTAIQIKELAQAGSELVR
ITVNTPEAAAAVPAIREQLDRMGVTVPLVGDFHYNGHLLLRDYPGCAESLSKYRINPGNVGQGAKRDTQFAQMIEAAARY
DKPVRIGVNWGSLDQDLLAKMMDENAARAEPWEAQSVMYEALIQSAIGSAERAVELGLGRDKIILSCKVSGVQDLIAVYQ
ELARRCDFALHLGLTEAGMGSKGIVASTAALSVLLQQGIGDTIRISLTPEPGASRTGEVIVGQEILQTMGLRSFAPMVIA
CPGCGRTTSTLFQELASQIQSYLRTQMPLWRDTYPGVEKMHVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFIDGV
KVKTLRGENIAQEFQQIVSDYVERSYGPNAVRAEATN

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=394, Percent_Identity=34.2639593908629, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 46753; Mature: 46753

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSDQVKSSSQIVSTVPVFGGHAPRRRSNAVDVRWGGQLVTIGGDAPVRVQSMTNTDTAD
CCCCHHCCCHHHHHCCCCCCCCCCCCCCCEEEEEECCEEEEECCCCCEEEEECCCCCHHH
AIGTAIQIKELAQAGSELVRITVNTPEAAAAVPAIREQLDRMGVTVPLVGDFHYNGHLLL
HHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEEE
RDYPGCAESLSKYRINPGNVGQGAKRDTQFAQMIEAAARYDKPVRIGVNWGSLDQDLLAK
ECCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHH
MMDENAARAEPWEAQSVMYEALIQSAIGSAERAVELGLGRDKIILSCKVSGVQDLIAVYQ
HHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCHHHHHHHHH
ELARRCDFALHLGLTEAGMGSKGIVASTAALSVLLQQGIGDTIRISLTPEPGASRTGEVI
HHHHHCCEEEEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEE
VGQEILQTMGLRSFAPMVIACPGCGRTTSTLFQELASQIQSYLRTQMPLWRDTYPGVEKM
ECHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
HVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFIDGVKVKTLRGENIAQEFQQIVSD
HEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEECCEEEEEECCCHHHHHHHHHHHH
YVERSYGPNAVRAEATN
HHHHCCCCCCCEECCCC
>Mature Secondary Structure
MHSDQVKSSSQIVSTVPVFGGHAPRRRSNAVDVRWGGQLVTIGGDAPVRVQSMTNTDTAD
CCCCHHCCCHHHHHCCCCCCCCCCCCCCCEEEEEECCEEEEECCCCCEEEEECCCCCHHH
AIGTAIQIKELAQAGSELVRITVNTPEAAAAVPAIREQLDRMGVTVPLVGDFHYNGHLLL
HHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCEEEE
RDYPGCAESLSKYRINPGNVGQGAKRDTQFAQMIEAAARYDKPVRIGVNWGSLDQDLLAK
ECCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHH
MMDENAARAEPWEAQSVMYEALIQSAIGSAERAVELGLGRDKIILSCKVSGVQDLIAVYQ
HHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEECCCHHHHHHHHH
ELARRCDFALHLGLTEAGMGSKGIVASTAALSVLLQQGIGDTIRISLTPEPGASRTGEVI
HHHHHCCEEEEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEE
VGQEILQTMGLRSFAPMVIACPGCGRTTSTLFQELASQIQSYLRTQMPLWRDTYPGVEKM
ECHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
HVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFIDGVKVKTLRGENIAQEFQQIVSD
HEEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCEEECCEEEEEECCCHHHHHHHHHHHH
YVERSYGPNAVRAEATN
HHHHCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA