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The map label for this gene is yjfH [C]

Identifier: 186476081

GI number: 186476081

Start: 1482185

End: 1482985

Strand: Reverse

Name: yjfH [C]

Synonym: Bphy_1323

Alternate gene names: 186476081

Gene position: 1482985-1482185 (Counterclockwise)

Preceding gene: 186476082

Following gene: 186476079

Centisome position: 42.62

GC content: 65.92

Gene sequence:

>801_bases
GTGAAAGCCATCACCTCGCGGGACAATCCGCTCTATAAGCGTCTGAAGGTTCTGGCCGGATCGACGCATCAACAGCGCAA
GAGCGGCCATGCGCTGCTCGAGGGATTTCACCTCGCGAGCGCCTACCTCGATGTGGCCGGGCAGCCGGAAACGTGCGTCG
TCACGGAAGGCGCGCTGCGCCACGACGAGGCGCAGGCGATCGTCGCGCGTATCGACGAACGGCGTATTGTGACGCTGCCG
GATGCACTGTTCGGGCAGCTGTCGAATGTGGTCAACGGCGTTGGCATGTTGCTGCTGGTCGAGAAACTCGATTTGCCGCT
TCCCGAGCGCGTGACGCACGGCTGCGTGGTGCTCGACGGCGTGCAGGATGCGGGCAACGTCGGTTCGATCCTGCGCAGCG
CGGCGGCGGCGGGGATCGGGCATGTCTTTTGCGCACCGGGCACCGCGTACGCGTGGTCGTCGAAGGTGCTGCGTTCGGCG
ATGGGTGCGCATTTCCTGCTGCAGATTCACGAGGACATCGAAGCGCCGACGCTGATCCAACTGCTCGACGTGCCTGTCGC
GATCACGGATTCGCACGGCGCCGAGGCGATTTACGAGACCGATCTGTCCGGGCCAGTCGCCTGGGTGTTCGGCAATGAGG
GGGCGGGCGTGTCGCAACCGTGGCGCGACGCCGTCACGTATCGGGTGACGATTCCGCAGCCGGGGGGGATGGAGTCGCTC
AATGTGGCAGCGGCCGCTGCCGTGTGTCTGTTCGAGCAATGCCGGCAGCAGCGTGGCAAGGGCGCGCGCACCGCCCGTTG
A

Upstream 100 bases:

>100_bases
GCGTTTCTCGCCGACGACAGCGCGCCGTTCTAAGCGGGCACCAACCCATCAAGCGCCTCCGGCGCTTTTCTTTTCACCGT
TTTTTTCTCGCTCGTCGCCT

Downstream 100 bases:

>100_bases
ACGTCACTGCTTCACTGCGTCGCCATCAATACGACGACCGGTCCAGCCTGATTTCCTGCAGGATGGTCGTCGCGATTTCC
TCGATCGACTTGTGCGTCGA

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKAITSRDNPLYKRLKVLAGSTHQQRKSGHALLEGFHLASAYLDVAGQPETCVVTEGALRHDEAQAIVARIDERRIVTLP
DALFGQLSNVVNGVGMLLLVEKLDLPLPERVTHGCVVLDGVQDAGNVGSILRSAAAAGIGHVFCAPGTAYAWSSKVLRSA
MGAHFLLQIHEDIEAPTLIQLLDVPVAITDSHGAEAIYETDLSGPVAWVFGNEGAGVSQPWRDAVTYRVTIPQPGGMESL
NVAAAAAVCLFEQCRQQRGKGARTAR

Sequences:

>Translated_266_residues
MKAITSRDNPLYKRLKVLAGSTHQQRKSGHALLEGFHLASAYLDVAGQPETCVVTEGALRHDEAQAIVARIDERRIVTLP
DALFGQLSNVVNGVGMLLLVEKLDLPLPERVTHGCVVLDGVQDAGNVGSILRSAAAAGIGHVFCAPGTAYAWSSKVLRSA
MGAHFLLQIHEDIEAPTLIQLLDVPVAITDSHGAEAIYETDLSGPVAWVFGNEGAGVSQPWRDAVTYRVTIPQPGGMESL
NVAAAAAVCLFEQCRQQRGKGARTAR
>Mature_266_residues
MKAITSRDNPLYKRLKVLAGSTHQQRKSGHALLEGFHLASAYLDVAGQPETCVVTEGALRHDEAQAIVARIDERRIVTLP
DALFGQLSNVVNGVGMLLLVEKLDLPLPERVTHGCVVLDGVQDAGNVGSILRSAAAAGIGHVFCAPGTAYAWSSKVLRSA
MGAHFLLQIHEDIEAPTLIQLLDVPVAITDSHGAEAIYETDLSGPVAWVFGNEGAGVSQPWRDAVTYRVTIPQPGGMESL
NVAAAAAVCLFEQCRQQRGKGARTAR

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=162, Percent_Identity=29.6296296296296, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28318; Mature: 28318

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAITSRDNPLYKRLKVLAGSTHQQRKSGHALLEGFHLASAYLDVAGQPETCVVTEGALR
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHH
HDEAQAIVARIDERRIVTLPDALFGQLSNVVNGVGMLLLVEKLDLPLPERVTHGCVVLDG
HHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEC
VQDAGNVGSILRSAAAAGIGHVFCAPGTAYAWSSKVLRSAMGAHFLLQIHEDIEAPTLIQ
CCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCHHHEEEEHHCCCCCHHHH
LLDVPVAITDSHGAEAIYETDLSGPVAWVFGNEGAGVSQPWRDAVTYRVTIPQPGGMESL
HHCCCEEEECCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCHH
NVAAAAAVCLFEQCRQQRGKGARTAR
HHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKAITSRDNPLYKRLKVLAGSTHQQRKSGHALLEGFHLASAYLDVAGQPETCVVTEGALR
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHH
HDEAQAIVARIDERRIVTLPDALFGQLSNVVNGVGMLLLVEKLDLPLPERVTHGCVVLDG
HHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEC
VQDAGNVGSILRSAAAAGIGHVFCAPGTAYAWSSKVLRSAMGAHFLLQIHEDIEAPTLIQ
CCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCHHHEEEEHHCCCCCHHHH
LLDVPVAITDSHGAEAIYETDLSGPVAWVFGNEGAGVSQPWRDAVTYRVTIPQPGGMESL
HHCCCEEEECCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCHH
NVAAAAAVCLFEQCRQQRGKGARTAR
HHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]