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The map label for this gene is 186475879

Identifier: 186475879

GI number: 186475879

Start: 1260671

End: 1261966

Strand: Direct

Name: 186475879

Synonym: Bphy_1114

Alternate gene names: NA

Gene position: 1260671-1261966 (Clockwise)

Preceding gene: 186475878

Following gene: 186475880

Centisome position: 36.23

GC content: 64.35

Gene sequence:

>1296_bases
ATGTGGTACGCATTCATCGATGGACAGCGGCGCCTCGCTCGCACGATTGCTAGCCAGATACAGTGCGGCGAAGTCTGGAG
TACGGCGACGGCAGCATCCGCGACGGGTCCGGGTGTCGCGGTGAGTGCGTTCCGTCACTGGCTCCTCAGGTCGACATGCG
CATACCGGCACGCACCGTCATTTGGAATTCGCGTCGTGGCGCACAGCGGAAACGGGATCGACATTACAGAAACCGTTGTC
GATTCCGTGCCGACGGGCACGCTGCGCAAGTTCACGCGGACGGGCACTGCGCGTCAGGATCACACATGGCCCGTCATGCT
TTGCGCACCGCTCGCGGGGCATCACGCCGTCATGTTGCGCGAAACCGTCGAGACGCTGCTCGAAGAGCGCGACGTGTTCG
TATCGGATTGGGCCGACGCACGCGATGTGCCAAACGAAGCCGGTCCTCTGTCGCTCGACGACTATGTGCTTGCACTGGAA
CGCTTCATGGATTCGGCCAGTGCATGCGGACCCCCGATACATGTCGTCGCCGTATGCCAGGCCACCGTACCCGCACTCGC
CGCCGCAGCGTTGCTCGCAAGCGTGCGCACAGTGCCCTTCGCCAGCCTCTCGCTGATCGGAGGGCCCATCGATACCCGGA
TCAGTCCAACCGTGATCGACCGCTTCGCCGCATCGCACACGCTCACATGGTTTCGCGAACATGTGATCGACGTCGTTCCC
CCGCCGTATCGCGGCGCAGGAAGGCGCGTCTATCCCGGATTCATCCAGCACGGCGCCCTCCTCTCGGCGCATCCGGAGCG
GCACGTCCGCCTCGAATCCGCGTACTGGTCGAACTGGCTGGCAGGCGACATGCGCGCCGCCGGCGAGGCGCTGCGGTCAC
TGAACGAGTACGCAGCCGTACTCGACATGGCGGAACGCTATTTCCTCGACATCCTTCGCATCGTTTTCCAGGAGCATTTG
CTGCCGCGCAATACCTGGTGGGTTCAGGGCCGGCGCGTCGAACCGAAAGCGCTCTGCCGCACACCGCTGTGCACCGTGGA
AGGCGAACTCGACGACATCACAGGCGCAGGCCAGACGCACTGCGCACACACATTGTGCGGCGCTTCCTCTTCGCCGCGCG
ACGGGCAGATCACTGTTCCTGCGTGCAATCACTATGACCTCTTCACCGGACCGCGCTGGCGCGATGTCGTCCATCCGGCG
CTGTGCAGCTTCTGGCGGGACGTCGAGGACGCGGCGTCGAGCATGCCCGTCAGGCAGGATAAAGACAGGCCGACCCGTCG
CAGGAGGCACGCGTGA

Upstream 100 bases:

>100_bases
GAACCCGGACGCAATCCCGACGCGTATGACCAGCGACACGGATGCGTTCTGGCGGGCGCCGCAGCGTTTTCTTCGGTACT
GACGGCAGACGGAGCTGATG

Downstream 100 bases:

>100_bases
CCCTACCGTTCAGACGCAGGCCGAGGAAGCGGCGCACCCGTTCGACGAACGTCAGCACACGGCATGGCGCGAACCGCATT
CGAGCCGCGCAACGCGAGGT

Product: polyhydroxyalkanoate depolymerase, intracellular

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; PHB De-Polymerase Domain-Containing Protein; Poly(3-Hydroxybutyrate) Depolymerase; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MWYAFIDGQRRLARTIASQIQCGEVWSTATAASATGPGVAVSAFRHWLLRSTCAYRHAPSFGIRVVAHSGNGIDITETVV
DSVPTGTLRKFTRTGTARQDHTWPVMLCAPLAGHHAVMLRETVETLLEERDVFVSDWADARDVPNEAGPLSLDDYVLALE
RFMDSASACGPPIHVVAVCQATVPALAAAALLASVRTVPFASLSLIGGPIDTRISPTVIDRFAASHTLTWFREHVIDVVP
PPYRGAGRRVYPGFIQHGALLSAHPERHVRLESAYWSNWLAGDMRAAGEALRSLNEYAAVLDMAERYFLDILRIVFQEHL
LPRNTWWVQGRRVEPKALCRTPLCTVEGELDDITGAGQTHCAHTLCGASSSPRDGQITVPACNHYDLFTGPRWRDVVHPA
LCSFWRDVEDAASSMPVRQDKDRPTRRRRHA

Sequences:

>Translated_431_residues
MWYAFIDGQRRLARTIASQIQCGEVWSTATAASATGPGVAVSAFRHWLLRSTCAYRHAPSFGIRVVAHSGNGIDITETVV
DSVPTGTLRKFTRTGTARQDHTWPVMLCAPLAGHHAVMLRETVETLLEERDVFVSDWADARDVPNEAGPLSLDDYVLALE
RFMDSASACGPPIHVVAVCQATVPALAAAALLASVRTVPFASLSLIGGPIDTRISPTVIDRFAASHTLTWFREHVIDVVP
PPYRGAGRRVYPGFIQHGALLSAHPERHVRLESAYWSNWLAGDMRAAGEALRSLNEYAAVLDMAERYFLDILRIVFQEHL
LPRNTWWVQGRRVEPKALCRTPLCTVEGELDDITGAGQTHCAHTLCGASSSPRDGQITVPACNHYDLFTGPRWRDVVHPA
LCSFWRDVEDAASSMPVRQDKDRPTRRRRHA
>Mature_431_residues
MWYAFIDGQRRLARTIASQIQCGEVWSTATAASATGPGVAVSAFRHWLLRSTCAYRHAPSFGIRVVAHSGNGIDITETVV
DSVPTGTLRKFTRTGTARQDHTWPVMLCAPLAGHHAVMLRETVETLLEERDVFVSDWADARDVPNEAGPLSLDDYVLALE
RFMDSASACGPPIHVVAVCQATVPALAAAALLASVRTVPFASLSLIGGPIDTRISPTVIDRFAASHTLTWFREHVIDVVP
PPYRGAGRRVYPGFIQHGALLSAHPERHVRLESAYWSNWLAGDMRAAGEALRSLNEYAAVLDMAERYFLDILRIVFQEHL
LPRNTWWVQGRRVEPKALCRTPLCTVEGELDDITGAGQTHCAHTLCGASSSPRDGQITVPACNHYDLFTGPRWRDVVHPA
LCSFWRDVEDAASSMPVRQDKDRPTRRRRHA

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 47611; Mature: 47611

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWYAFIDGQRRLARTIASQIQCGEVWSTATAASATGPGVAVSAFRHWLLRSTCAYRHAPS
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
FGIRVVAHSGNGIDITETVVDSVPTGTLRKFTRTGTARQDHTWPVMLCAPLAGHHAVMLR
CCEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHH
ETVETLLEERDVFVSDWADARDVPNEAGPLSLDDYVLALERFMDSASACGPPIHVVAVCQ
HHHHHHHHHCCCEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHH
ATVPALAAAALLASVRTVPFASLSLIGGPIDTRISPTVIDRFAASHTLTWFREHVIDVVP
HHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
PPYRGAGRRVYPGFIQHGALLSAHPERHVRLESAYWSNWLAGDMRAAGEALRSLNEYAAV
CCCCCCCCEECCHHHHCCCEECCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDMAERYFLDILRIVFQEHLLPRNTWWVQGRRVEPKALCRTPLCTVEGELDDITGAGQTH
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHCCCCEEECCCCHHCCCCCCHH
CAHTLCGASSSPRDGQITVPACNHYDLFTGPRWRDVVHPALCSFWRDVEDAASSMPVRQD
HHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KDRPTRRRRHA
CCCCHHHHCCC
>Mature Secondary Structure
MWYAFIDGQRRLARTIASQIQCGEVWSTATAASATGPGVAVSAFRHWLLRSTCAYRHAPS
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
FGIRVVAHSGNGIDITETVVDSVPTGTLRKFTRTGTARQDHTWPVMLCAPLAGHHAVMLR
CCEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHH
ETVETLLEERDVFVSDWADARDVPNEAGPLSLDDYVLALERFMDSASACGPPIHVVAVCQ
HHHHHHHHHCCCEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHH
ATVPALAAAALLASVRTVPFASLSLIGGPIDTRISPTVIDRFAASHTLTWFREHVIDVVP
HHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
PPYRGAGRRVYPGFIQHGALLSAHPERHVRLESAYWSNWLAGDMRAAGEALRSLNEYAAV
CCCCCCCCEECCHHHHCCCEECCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDMAERYFLDILRIVFQEHLLPRNTWWVQGRRVEPKALCRTPLCTVEGELDDITGAGQTH
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHCCCCEEECCCCHHCCCCCCHH
CAHTLCGASSSPRDGQITVPACNHYDLFTGPRWRDVVHPALCSFWRDVEDAASSMPVRQD
HHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KDRPTRRRRHA
CCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA