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The map label for this gene is groL [H]

Identifier: 186475878

GI number: 186475878

Start: 1258806

End: 1260428

Strand: Direct

Name: groL [H]

Synonym: Bphy_1113

Alternate gene names: 186475878

Gene position: 1258806-1260428 (Clockwise)

Preceding gene: 186475874

Following gene: 186475879

Centisome position: 36.18

GC content: 66.17

Gene sequence:

>1623_bases
GTGTCACACAAGACGCTTGTATTTCATCAGGATGCGCGGCAATTGCTGCTCAACGGCGTGAACGCGCTCGCTGAAGCCGT
CAAGGTCACGCTCGGCCCAGGCGGCCGCAATGTGATCGTCGAGCGCGTTCCAGGTGCGCCGCTCGTCGCAAACTCCGGTG
TGGTGGTCGCGGGATCCGTCGACCTGTCCGATCCGTACGAGGAAATGGGCGCGCAGTTGCTACGCGAGGTCGCAACCCGC
ACGAGCGAAATCGCGGGGGACGGCACGACGACAGCCACGACACTCGCACAGGCGATCGTCGTCGAAGGCAACAAATGCGT
AACCGCGGGGCACGATCCGATGGCGCTCAAGCGTGCCATCGAAGACGCGGGCAAACAGGTCGTCGCGGAGCTCCACAGGC
TCGCACGACCCTGTTCGACCGTCGACGAAATGCGGCAGATCGCGACGATTTCCGCGAGCGGCGACGCGAGCATCGGCACG
CTGGTGGCCGACGCAGTGGCGCGCGTCGGCAAGGAAGGCGCGATCAGCGTCGAGGACGGCTCCAAACTCGACGACGAACT
CGAGACGGTCGACGGGTCCCTGCTGGACCGGGGTCTCGTCTCGCCGCTCTTTCTCGGCGAAGATCAACGCAACGTCGTGC
TCGAAGAGCCGCGCGTGTTGCTGTGCGATATGAACATCTCGTCCATCCCACAGCTTCTGCCCATGCTGGAGGCGGTCATG
GGAACGGGCAAGCCGCTGTTCGTCATTGCCAACGAGATCGAAGGCGAAGCGCTCGCGACGCTCGTCGTCAATCATCTGCG
CGGCACGTTCAAATCCTGTGCGATCCGCTCGCCGGGCTTCGGCGAAAGCCGCACCGAGCAACTGGGCGATCTCGCCGCGC
TGACGGGGGCTACCGTCATCTCGGCGCAGGCGGGACGCACGCTCGAGAACGCCACCGTCGAGGACCTCGGTGCTGCGCGG
CGCATCGAAGTGACACGCGACACGACGACCATCGTCGCAGGCAGCGGCGATCATGCGCGCGTCGCGGACCGCATAGCCGG
GCTGAAGAAGCGCATTGACGCAAGCGCGGATGGATATGAGCGCGAGAATCTCACCCGGCGCCTCGCGAAGCTTTCGGGCG
GTGTTGCCGTAATCCGCGTCGGTGCCGCAACGGAAACCGCGCTGCGCGAGCGGAAGAACCGCTTCGAAGACGCGCTGCAT
TCGACGCGCGCCGCCGTCGAAGAAGGCTTCGTGCCGGGCGGCGGCGTCGCGCTGCTGCGCGCGCGCCGGGTGCTCGAACA
CGACGGACAGACGCAGGCGCAGACACTCGGCGCACGGATCGTGTATGAAGCGCTTGCCGCGCCGCTGCGGCAAATCGCGC
AAAACGCGGGGGCGGATGCGCAGGCCGTCGTGCACGCCGTCGCTGAGGCGAGCGATGCGTTCGGCTACGATGCCGCCCAC
GGCGACTATGGCGATATGATCGAGCGAGGCATCGTCGATCCCGTGAAGGTGGCGCGCACCGCTCTGCAGAACGCCGTGTC
GGTCGCCTCGCTCCTGCTGACCACCGACTGCATGATCGTGCATCCGGCGAAGGATCGCACGGGTAGTGCAGGTCGCGGCG
ACTGGAGCCGCGAATTCGCATAA

Upstream 100 bases:

>100_bases
TCAATCGCCCCCCCGCCCGCGGCGACTTAAATAGGCAGACAGCCCACTACCGTTTTGCGCACTGCATGCGCTATCGCCCA
CGCACGCCCGGAGGTCTGCC

Downstream 100 bases:

>100_bases
GCGATGTGCCGCAGCGCGGTCTGCGTCTTCCGTCATGTCACGCTGTGTCGGATCGCAGGCTATCGCCCCTTACGCTTTCC
ACCTTTGTGCGACGCGAAGT

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60 [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSHKTLVFHQDARQLLLNGVNALAEAVKVTLGPGGRNVIVERVPGAPLVANSGVVVAGSVDLSDPYEEMGAQLLREVATR
TSEIAGDGTTTATTLAQAIVVEGNKCVTAGHDPMALKRAIEDAGKQVVAELHRLARPCSTVDEMRQIATISASGDASIGT
LVADAVARVGKEGAISVEDGSKLDDELETVDGSLLDRGLVSPLFLGEDQRNVVLEEPRVLLCDMNISSIPQLLPMLEAVM
GTGKPLFVIANEIEGEALATLVVNHLRGTFKSCAIRSPGFGESRTEQLGDLAALTGATVISAQAGRTLENATVEDLGAAR
RIEVTRDTTTIVAGSGDHARVADRIAGLKKRIDASADGYERENLTRRLAKLSGGVAVIRVGAATETALRERKNRFEDALH
STRAAVEEGFVPGGGVALLRARRVLEHDGQTQAQTLGARIVYEALAAPLRQIAQNAGADAQAVVHAVAEASDAFGYDAAH
GDYGDMIERGIVDPVKVARTALQNAVSVASLLLTTDCMIVHPAKDRTGSAGRGDWSREFA

Sequences:

>Translated_540_residues
MSHKTLVFHQDARQLLLNGVNALAEAVKVTLGPGGRNVIVERVPGAPLVANSGVVVAGSVDLSDPYEEMGAQLLREVATR
TSEIAGDGTTTATTLAQAIVVEGNKCVTAGHDPMALKRAIEDAGKQVVAELHRLARPCSTVDEMRQIATISASGDASIGT
LVADAVARVGKEGAISVEDGSKLDDELETVDGSLLDRGLVSPLFLGEDQRNVVLEEPRVLLCDMNISSIPQLLPMLEAVM
GTGKPLFVIANEIEGEALATLVVNHLRGTFKSCAIRSPGFGESRTEQLGDLAALTGATVISAQAGRTLENATVEDLGAAR
RIEVTRDTTTIVAGSGDHARVADRIAGLKKRIDASADGYERENLTRRLAKLSGGVAVIRVGAATETALRERKNRFEDALH
STRAAVEEGFVPGGGVALLRARRVLEHDGQTQAQTLGARIVYEALAAPLRQIAQNAGADAQAVVHAVAEASDAFGYDAAH
GDYGDMIERGIVDPVKVARTALQNAVSVASLLLTTDCMIVHPAKDRTGSAGRGDWSREFA
>Mature_539_residues
SHKTLVFHQDARQLLLNGVNALAEAVKVTLGPGGRNVIVERVPGAPLVANSGVVVAGSVDLSDPYEEMGAQLLREVATRT
SEIAGDGTTTATTLAQAIVVEGNKCVTAGHDPMALKRAIEDAGKQVVAELHRLARPCSTVDEMRQIATISASGDASIGTL
VADAVARVGKEGAISVEDGSKLDDELETVDGSLLDRGLVSPLFLGEDQRNVVLEEPRVLLCDMNISSIPQLLPMLEAVMG
TGKPLFVIANEIEGEALATLVVNHLRGTFKSCAIRSPGFGESRTEQLGDLAALTGATVISAQAGRTLENATVEDLGAARR
IEVTRDTTTIVAGSGDHARVADRIAGLKKRIDASADGYERENLTRRLAKLSGGVAVIRVGAATETALRERKNRFEDALHS
TRAAVEEGFVPGGGVALLRARRVLEHDGQTQAQTLGARIVYEALAAPLRQIAQNAGADAQAVVHAVAEASDAFGYDAAHG
DYGDMIERGIVDPVKVARTALQNAVSVASLLLTTDCMIVHPAKDRTGSAGRGDWSREFA

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=528, Percent_Identity=43.75, Blast_Score=431, Evalue=1e-120,
Organism=Homo sapiens, GI31542947, Length=528, Percent_Identity=43.75, Blast_Score=431, Evalue=1e-120,
Organism=Escherichia coli, GI1790586, Length=527, Percent_Identity=51.0436432637571, Blast_Score=528, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17555558, Length=526, Percent_Identity=43.3460076045627, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI193210679, Length=209, Percent_Identity=44.4976076555024, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323288, Length=525, Percent_Identity=46.6666666666667, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI45550936, Length=521, Percent_Identity=43.9539347408829, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI45550132, Length=521, Percent_Identity=43.9539347408829, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI45550935, Length=521, Percent_Identity=43.9539347408829, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24641193, Length=525, Percent_Identity=43.4285714285714, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24641191, Length=525, Percent_Identity=43.4285714285714, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI17864606, Length=530, Percent_Identity=39.622641509434, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24584129, Length=524, Percent_Identity=35.3053435114504, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI19921262, Length=524, Percent_Identity=35.3053435114504, Blast_Score=296, Evalue=2e-80,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 56681; Mature: 56550

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKTLVFHQDARQLLLNGVNALAEAVKVTLGPGGRNVIVERVPGAPLVANSGVVVAGSV
CCCCEEEEHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCCEEECCCEEEEECC
DLSDPYEEMGAQLLREVATRTSEIAGDGTTTATTLAQAIVVEGNKCVTAGHDPMALKRAI
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEECCEEEECCCCHHHHHHHH
EDAGKQVVAELHRLARPCSTVDEMRQIATISASGDASIGTLVADAVARVGKEGAISVEDG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCEECCCC
SKLDDELETVDGSLLDRGLVSPLFLGEDQRNVVLEEPRVLLCDMNISSIPQLLPMLEAVM
CCCHHHHHHHCHHHHHHHCCCHHHCCCCCCCEEECCCCEEEECCCHHHHHHHHHHHHHHH
GTGKPLFVIANEIEGEALATLVVNHLRGTFKSCAIRSPGFGESRTEQLGDLAALTGATVI
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHE
SAQAGRTLENATVEDLGAARRIEVTRDTTTIVAGSGDHARVADRIAGLKKRIDASADGYE
ECCCCCCHHCCCHHHCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHH
RENLTRRLAKLSGGVAVIRVGAATETALRERKNRFEDALHSTRAAVEEGFVPGGGVALLR
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ARRVLEHDGQTQAQTLGARIVYEALAAPLRQIAQNAGADAQAVVHAVAEASDAFGYDAAH
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
GDYGDMIERGIVDPVKVARTALQNAVSVASLLLTTDCMIVHPAKDRTGSAGRGDWSREFA
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SHKTLVFHQDARQLLLNGVNALAEAVKVTLGPGGRNVIVERVPGAPLVANSGVVVAGSV
CCCEEEEHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCCEEECCCEEEEECC
DLSDPYEEMGAQLLREVATRTSEIAGDGTTTATTLAQAIVVEGNKCVTAGHDPMALKRAI
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEECCEEEECCCCHHHHHHHH
EDAGKQVVAELHRLARPCSTVDEMRQIATISASGDASIGTLVADAVARVGKEGAISVEDG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCEECCCC
SKLDDELETVDGSLLDRGLVSPLFLGEDQRNVVLEEPRVLLCDMNISSIPQLLPMLEAVM
CCCHHHHHHHCHHHHHHHCCCHHHCCCCCCCEEECCCCEEEECCCHHHHHHHHHHHHHHH
GTGKPLFVIANEIEGEALATLVVNHLRGTFKSCAIRSPGFGESRTEQLGDLAALTGATVI
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHE
SAQAGRTLENATVEDLGAARRIEVTRDTTTIVAGSGDHARVADRIAGLKKRIDASADGYE
ECCCCCCHHCCCHHHCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHH
RENLTRRLAKLSGGVAVIRVGAATETALRERKNRFEDALHSTRAAVEEGFVPGGGVALLR
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ARRVLEHDGQTQAQTLGARIVYEALAAPLRQIAQNAGADAQAVVHAVAEASDAFGYDAAH
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
GDYGDMIERGIVDPVKVARTALQNAVSVASLLLTTDCMIVHPAKDRTGSAGRGDWSREFA
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA