Click here to switch to the map view.

The map label for this gene is holB [H]

Identifier: 186475809

GI number: 186475809

Start: 1173278

End: 1174327

Strand: Direct

Name: holB [H]

Synonym: Bphy_1044

Alternate gene names: 186475809

Gene position: 1173278-1174327 (Clockwise)

Preceding gene: 186475808

Following gene: 186475810

Centisome position: 33.72

GC content: 68.1

Gene sequence:

>1050_bases
ATGATTTATCCGTGGCAAACCGACGACTGGAGCCGCCTTCAGCAACTGCGCGCTCACTGGCCGCATGCGTTGCTGCTCCA
CGGGCAGGCCGGCATCGGCAAGCTGCGTTTTGCCCAGCATCTCGCGCAAGGCCTGCTGTGCGAATCGGCGCTGCCGAACG
GCGAGCCGTGCGGCACGTGCGCGGCGTGCAACTGGTTCGTGCAGGGCAATCATCCCGATTACCGGATTGTGGTGCCGGAA
GCGCTTGCGGCCGAAGCGGGCTTCTCCAGTGCGGCCGCCGATGAAAAGGCCGAGAAGGGCGACGCCGACGACAGCGGTAA
AAAAACGCGCACGCCAAGCAAGGAAATCAAGATCGAGCAGGTGCGGGCGCTGCTGGACTTCTGCGGCGTGGGCTCGCATC
GCGGCGGCGTGCGCGTCGTCGTGCTGTACCCGGCAGAGGCGCTGAACGTCGCGGCGGCCAACGCGCTGCTGAAAACGCTC
GAAGAGCCGCCTGCGGGCGTGGTGTTTCTGATGGTGTCGGCGCGCATCGACCGTCTGCTGCCGACCATCATCAGCCGCTG
CCGCCAATGGCCGATGACAACGCCGTCGCCGGAATCCGCCACCGCATGGCTCGCGCAACAGGGTGTCGACGACCCGCCGG
GCCTGCTCGCGGAAACGGGCGGCGCGCCGCTGGCCGCACTGGCGCTCGCGAGCGACGAAAACCGCCCGTTGCGCGACTGG
ACGCTCAAACAACTGGCCGCCGGCCCGAATTGCGACGCCTTCGCGTGCGGAGAGACGCTGCAGAAGCTGCCTGTGCCGAT
CGTGCTCGGCTGGCTGCAGCGGTGGACGTACGACCTGCTGGCCCAGTCGACGGTCGGCGCGGCGGGCACGCCGCGCTATT
TCCCGGCGGCGTCGGCGGCGCTCGCGCGTTGTGCCTCGCAGGCCGATGCGACCGCCTTCGCGCGCTACATGCGAACGGTC
ACGCGGCAGCGCGCGGTCGAAAACCATCCGCTCAACGCACGGTTGGTGTTCGAGGAACTGTTTCTCGGCTACCGCGACCT
GTTCGCGTAG

Upstream 100 bases:

>100_bases
TTTGATTTTCCTGTATTACAAGCCGGCCTATCACAGAGTGCATGCTCGCCGGCCGATACACAGGAAATCGTCTTCGAACC
ATTGAATTGAAGAACCAACG

Downstream 100 bases:

>100_bases
CGCGCGGTCAGCCCCGTCGTCACCGCTATTGGTTTTTTAAGCAGGACTTCACCATGTTTGTCGATTCCCACTGCCATATC
AATTTCGAAGGACTCGGCGA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MIYPWQTDDWSRLQQLRAHWPHALLLHGQAGIGKLRFAQHLAQGLLCESALPNGEPCGTCAACNWFVQGNHPDYRIVVPE
ALAAEAGFSSAAADEKAEKGDADDSGKKTRTPSKEIKIEQVRALLDFCGVGSHRGGVRVVVLYPAEALNVAAANALLKTL
EEPPAGVVFLMVSARIDRLLPTIISRCRQWPMTTPSPESATAWLAQQGVDDPPGLLAETGGAPLAALALASDENRPLRDW
TLKQLAAGPNCDAFACGETLQKLPVPIVLGWLQRWTYDLLAQSTVGAAGTPRYFPAASAALARCASQADATAFARYMRTV
TRQRAVENHPLNARLVFEELFLGYRDLFA

Sequences:

>Translated_349_residues
MIYPWQTDDWSRLQQLRAHWPHALLLHGQAGIGKLRFAQHLAQGLLCESALPNGEPCGTCAACNWFVQGNHPDYRIVVPE
ALAAEAGFSSAAADEKAEKGDADDSGKKTRTPSKEIKIEQVRALLDFCGVGSHRGGVRVVVLYPAEALNVAAANALLKTL
EEPPAGVVFLMVSARIDRLLPTIISRCRQWPMTTPSPESATAWLAQQGVDDPPGLLAETGGAPLAALALASDENRPLRDW
TLKQLAAGPNCDAFACGETLQKLPVPIVLGWLQRWTYDLLAQSTVGAAGTPRYFPAASAALARCASQADATAFARYMRTV
TRQRAVENHPLNARLVFEELFLGYRDLFA
>Mature_349_residues
MIYPWQTDDWSRLQQLRAHWPHALLLHGQAGIGKLRFAQHLAQGLLCESALPNGEPCGTCAACNWFVQGNHPDYRIVVPE
ALAAEAGFSSAAADEKAEKGDADDSGKKTRTPSKEIKIEQVRALLDFCGVGSHRGGVRVVVLYPAEALNVAAANALLKTL
EEPPAGVVFLMVSARIDRLLPTIISRCRQWPMTTPSPESATAWLAQQGVDDPPGLLAETGGAPLAALALASDENRPLRDW
TLKQLAAGPNCDAFACGETLQKLPVPIVLGWLQRWTYDLLAQSTVGAAGTPRYFPAASAALARCASQADATAFARYMRTV
TRQRAVENHPLNARLVFEELFLGYRDLFA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=349, Percent_Identity=30.0859598853868, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37774; Mature: 37774

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYPWQTDDWSRLQQLRAHWPHALLLHGQAGIGKLRFAQHLAQGLLCESALPNGEPCGTC
CCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCH
AACNWFVQGNHPDYRIVVPEALAAEAGFSSAAADEKAEKGDADDSGKKTRTPSKEIKIEQ
HHEEEEEECCCCCEEEECCHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
VRALLDFCGVGSHRGGVRVVVLYPAEALNVAAANALLKTLEEPPAGVVFLMVSARIDRLL
HHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
PTIISRCRQWPMTTPSPESATAWLAQQGVDDPPGLLAETGGAPLAALALASDENRPLRDW
HHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCEEEEEEECCCCCCCHHH
TLKQLAAGPNCDAFACGETLQKLPVPIVLGWLQRWTYDLLAQSTVGAAGTPRYFPAASAA
HHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
LARCASQADATAFARYMRTVTRQRAVENHPLNARLVFEELFLGYRDLFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIYPWQTDDWSRLQQLRAHWPHALLLHGQAGIGKLRFAQHLAQGLLCESALPNGEPCGTC
CCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCH
AACNWFVQGNHPDYRIVVPEALAAEAGFSSAAADEKAEKGDADDSGKKTRTPSKEIKIEQ
HHEEEEEECCCCCEEEECCHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
VRALLDFCGVGSHRGGVRVVVLYPAEALNVAAANALLKTLEEPPAGVVFLMVSARIDRLL
HHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
PTIISRCRQWPMTTPSPESATAWLAQQGVDDPPGLLAETGGAPLAALALASDENRPLRDW
HHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCEEEEEEECCCCCCCHHH
TLKQLAAGPNCDAFACGETLQKLPVPIVLGWLQRWTYDLLAQSTVGAAGTPRYFPAASAA
HHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
LARCASQADATAFARYMRTVTRQRAVENHPLNARLVFEELFLGYRDLFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]