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The map label for this gene is ygfZ [C]

Identifier: 186475806

GI number: 186475806

Start: 1170425

End: 1171474

Strand: Reverse

Name: ygfZ [C]

Synonym: Bphy_1041

Alternate gene names: 186475806

Gene position: 1171474-1170425 (Counterclockwise)

Preceding gene: 186475816

Following gene: 186475805

Centisome position: 33.67

GC content: 68.38

Gene sequence:

>1050_bases
ATGAACGCACCGCTTGCTTCTGCTCCCGGCACCGCTGTCCCCGCTCCCGCATTGCTTCCCGCGTTCCCGCGCCCCACGCG
TGACGAGTTCGACGCCGTGCGGGCGGCGGGCGCGTTCATGCCGCTGCCGCAGTTCGGCGTGATCGACGCGACGGGCGACG
ACGCCGCGTCGTTCCTGCACTCGCAACTCACCAACGACACACAGCACCTGGACGCGGCGACGGCGCGCCTTGCCGGCTAC
TGCTCGCCGAAGGGCCGCCTGCTGGCGTCGTTCCTGGTGTGGTGCAGCGGCGAGTCGATCCGGATGCTGGTGTCGAAGGA
CGTGCAGCCCGCCGTGCAAAAACGCCTGTCGATGTTCGTGCTGCGCGCGAAGGCGAAGCTCAGCGACGCATCGGGCGACA
CCCTCGCGATTGGACTCGCCGGCGACGTGCGCAAGGCGCTCTCGGGTATCTTCGATGCGATTCCCGACGGCGTGCACGTG
AAGGTCGACGGGCCGGCGGGATCGCTGGTGCGCGTGCCGGACGCCGCGGGACGTCTGCGCTATGTCTGGGTCGGCCCGAA
GGCCGAAGTCGAAGCGTGTCTTCCCGCGCTCGAAACGAAGCTGCGCCGCGTGTCGCCTGGCGTGTGGGACTGGCTCGACA
TCCGCGCAGGCGAACCGCGCATCACGCAGCGCGTGGTCGAGCAGTTCGTGCCGCAGATGATCAATTTCGACGTGCTCGGC
GGCGTCAATTTCCGCAAGGGCTGCTATCCGGGCCAGGAAGTGGTCGCACGCAGCCAGTACCGCGGCACGATCAAGCGCCG
CATGTCGCTGGCGAATGTTGCCGGCGAGACGGAAAGCGTCGTGCCGGGCGCCGAGCTATTCCATTCGGACGATCCGGGCC
AGCCGTGCGGGATGCTCGTCAATACGGCGGCCGCGCCTGACGGCGGCGTCGATGCGCTCGTCGAGATCAAGCTGGCCGCG
CTCGAAAACGGCACCGTTCATCTGGGCGCCGCCGATGGCCCCGCGCTGACCTTCCTCCCCTTGCCGTACGCATTGCCGAC
AGAAGTCTGA

Upstream 100 bases:

>100_bases
AGACATTTCTTCAGGAGGGACATGGAAACGTGGTTCGAGTGAAGCCAATATAATACTTGCTTGCCTCCGCCAAAGTCAGA
ATTGACTGTTCCCCGAATCC

Downstream 100 bases:

>100_bases
GCCTCCCTTCGTTCACGTCGCCTTCGACGTCGCGCCTCCGACGCAGGCGCGACGCGTGGTTCCCGTATCCAGGCTGTCCC
GCTAAAGAGACTTTCGCTGA

Product: putative glycine cleavage T protein (aminomethyltransferase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MNAPLASAPGTAVPAPALLPAFPRPTRDEFDAVRAAGAFMPLPQFGVIDATGDDAASFLHSQLTNDTQHLDAATARLAGY
CSPKGRLLASFLVWCSGESIRMLVSKDVQPAVQKRLSMFVLRAKAKLSDASGDTLAIGLAGDVRKALSGIFDAIPDGVHV
KVDGPAGSLVRVPDAAGRLRYVWVGPKAEVEACLPALETKLRRVSPGVWDWLDIRAGEPRITQRVVEQFVPQMINFDVLG
GVNFRKGCYPGQEVVARSQYRGTIKRRMSLANVAGETESVVPGAELFHSDDPGQPCGMLVNTAAAPDGGVDALVEIKLAA
LENGTVHLGAADGPALTFLPLPYALPTEV

Sequences:

>Translated_349_residues
MNAPLASAPGTAVPAPALLPAFPRPTRDEFDAVRAAGAFMPLPQFGVIDATGDDAASFLHSQLTNDTQHLDAATARLAGY
CSPKGRLLASFLVWCSGESIRMLVSKDVQPAVQKRLSMFVLRAKAKLSDASGDTLAIGLAGDVRKALSGIFDAIPDGVHV
KVDGPAGSLVRVPDAAGRLRYVWVGPKAEVEACLPALETKLRRVSPGVWDWLDIRAGEPRITQRVVEQFVPQMINFDVLG
GVNFRKGCYPGQEVVARSQYRGTIKRRMSLANVAGETESVVPGAELFHSDDPGQPCGMLVNTAAAPDGGVDALVEIKLAA
LENGTVHLGAADGPALTFLPLPYALPTEV
>Mature_349_residues
MNAPLASAPGTAVPAPALLPAFPRPTRDEFDAVRAAGAFMPLPQFGVIDATGDDAASFLHSQLTNDTQHLDAATARLAGY
CSPKGRLLASFLVWCSGESIRMLVSKDVQPAVQKRLSMFVLRAKAKLSDASGDTLAIGLAGDVRKALSGIFDAIPDGVHV
KVDGPAGSLVRVPDAAGRLRYVWVGPKAEVEACLPALETKLRRVSPGVWDWLDIRAGEPRITQRVVEQFVPQMINFDVLG
GVNFRKGCYPGQEVVARSQYRGTIKRRMSLANVAGETESVVPGAELFHSDDPGQPCGMLVNTAAAPDGGVDALVEIKLAA
LENGTVHLGAADGPALTFLPLPYALPTEV

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=326, Percent_Identity=25.7668711656442, Blast_Score=101, Evalue=9e-23,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 36866; Mature: 36866

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPLASAPGTAVPAPALLPAFPRPTRDEFDAVRAAGAFMPLPQFGVIDATGDDAASFLH
CCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHH
SQLTNDTQHLDAATARLAGYCSPKGRLLASFLVWCSGESIRMLVSKDVQPAVQKRLSMFV
HHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEHHCCCCHHHHHHHHHHH
LRAKAKLSDASGDTLAIGLAGDVRKALSGIFDAIPDGVHVKVDGPAGSLVRVPDAAGRLR
HHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCCCCCEE
YVWVGPKAEVEACLPALETKLRRVSPGVWDWLDIRAGEPRITQRVVEQFVPQMINFDVLG
EEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCEEC
GVNFRKGCYPGQEVVARSQYRGTIKRRMSLANVAGETESVVPGAELFHSDDPGQPCGMLV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCEE
NTAAAPDGGVDALVEIKLAALENGTVHLGAADGPALTFLPLPYALPTEV
ECCCCCCCCCCCEEEEEEEEECCCEEEEECCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MNAPLASAPGTAVPAPALLPAFPRPTRDEFDAVRAAGAFMPLPQFGVIDATGDDAASFLH
CCCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHH
SQLTNDTQHLDAATARLAGYCSPKGRLLASFLVWCSGESIRMLVSKDVQPAVQKRLSMFV
HHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEHHCCCCHHHHHHHHHHH
LRAKAKLSDASGDTLAIGLAGDVRKALSGIFDAIPDGVHVKVDGPAGSLVRVPDAAGRLR
HHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCCCCCEE
YVWVGPKAEVEACLPALETKLRRVSPGVWDWLDIRAGEPRITQRVVEQFVPQMINFDVLG
EEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCEEC
GVNFRKGCYPGQEVVARSQYRGTIKRRMSLANVAGETESVVPGAELFHSDDPGQPCGMLV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCEE
NTAAAPDGGVDALVEIKLAALENGTVHLGAADGPALTFLPLPYALPTEV
ECCCCCCCCCCCEEEEEEEEECCCEEEEECCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]