BacMap

Definition	Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence.
Accession	NC_010622
Length	3,479,187

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The map label for this gene is butA [H]

Identifier: 186475803

GI number: 186475803

Start: 1167566

End: 1168435

Strand: Direct

Name: butA [H]

Synonym: Bphy_1038

Alternate gene names: 186475803

Gene position: 1167566-1168435 (Clockwise)

Preceding gene: 186475802

Following gene: 186475804

Centisome position: 33.56

GC content: 66.32

Gene sequence:

>870_bases
ATGTTCGAATTCGACGGCAAGGTCGCGGTGATAACAGGCGCGGGAAGCGGCTTTGGCCGTGCGTTTGCGGAGAAGGGCGC
GTCGCTCGGCATGAAGCTCGTGCTGGCGGATGTCGACGAAGGCGCGCTCGCACGAACTGTCGATACGTTGCGCGCAGCGG
GCGCCGAGGTGATCGGCGTGCGCACGGACGTGTCGAATGGCGCGCAGGTTCAGGCGCTCGCCGATGCGGCGCTCGAAGCG
TTCGGCAAGGTGCATCTGCTGTTCAACAACGCGGGCGTCGGCGCCGGCGGCTTTCTATGGGAAAGCTCGGCGAACGACTG
GGCGTGGGTGTTCGGCGTCAACGTGATGGGCGTCGCGCACGGCGTGCGCGTCTTCGCGCCCATCATGCTCGGGCAGAACG
AAGCGGCGCATATCGTCAATACGGCGTCGGTGGCGGGCCTGCTGTCGCCGCCGTCGATGGGCATCTACAACGCGTCGAAG
CATGCGGTGGTTTCGCTGACCGAGACGCTGTATCACGACCTGCGCAATGCGGGCGGCGAGGTGGGCTGTTCGCTGCTGTG
TCCCGCGTTCGTGCCCACAGGTATCGCCGACGCCGAACGCGTGCGCCCCGAGGCGTTGCGCAACGAAGCGCAACCCACGC
GCTCGCAGCTCGCCGCCGACAGGCAACTGCAGCGTGCAGTGCGGTCCGGCAAGTTGGGCGCGACCGACGTCGCGACGCTC
ACCTTCGAAGCGATCGCGGAACGCCGCTTCTACATCCTCACGCATCCCGCCATTCTCGCGACGGTGCGGCTTCGTCACGA
GGACATCGAACTGCAGCGCAATCCGACCGATCCGTTGTCGCTGAAGCCCGAAGTCAAGGAAGCCCGCTAA

Upstream 100 bases:

>100_bases
TCGGCATGCTCAAGGGGCGCAATTTCGGCAAGCAGCTGGTCAAGCTGATCTGAACCGGCGCATGGCGGGCCAGCCCAAGC
AATCCAACGAAGGAAACGGC

Downstream 100 bases:

>100_bases
CGGTTATCGCGAGCGCGCCGGGCTATGTTCGTGGCATCATGTCGCGCTCATTCAATGTCCGGCGCTGGCGCGCCATCGTC
CGATCATGCCGTTGAACCCG

Product: hypothetical protein

Products: NA

Alternate protein names: Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MFEFDGKVAVITGAGSGFGRAFAEKGASLGMKLVLADVDEGALARTVDTLRAAGAEVIGVRTDVSNGAQVQALADAALEA
FGKVHLLFNNAGVGAGGFLWESSANDWAWVFGVNVMGVAHGVRVFAPIMLGQNEAAHIVNTASVAGLLSPPSMGIYNASK
HAVVSLTETLYHDLRNAGGEVGCSLLCPAFVPTGIADAERVRPEALRNEAQPTRSQLAADRQLQRAVRSGKLGATDVATL
TFEAIAERRFYILTHPAILATVRLRHEDIELQRNPTDPLSLKPEVKEAR

Sequences:

>Translated_289_residues
MFEFDGKVAVITGAGSGFGRAFAEKGASLGMKLVLADVDEGALARTVDTLRAAGAEVIGVRTDVSNGAQVQALADAALEA
FGKVHLLFNNAGVGAGGFLWESSANDWAWVFGVNVMGVAHGVRVFAPIMLGQNEAAHIVNTASVAGLLSPPSMGIYNASK
HAVVSLTETLYHDLRNAGGEVGCSLLCPAFVPTGIADAERVRPEALRNEAQPTRSQLAADRQLQRAVRSGKLGATDVATL
TFEAIAERRFYILTHPAILATVRLRHEDIELQRNPTDPLSLKPEVKEAR
>Mature_289_residues
MFEFDGKVAVITGAGSGFGRAFAEKGASLGMKLVLADVDEGALARTVDTLRAAGAEVIGVRTDVSNGAQVQALADAALEA
FGKVHLLFNNAGVGAGGFLWESSANDWAWVFGVNVMGVAHGVRVFAPIMLGQNEAAHIVNTASVAGLLSPPSMGIYNASK
HAVVSLTETLYHDLRNAGGEVGCSLLCPAFVPTGIADAERVRPEALRNEAQPTRSQLAADRQLQRAVRSGKLGATDVATL
TFEAIAERRFYILTHPAILATVRLRHEDIELQRNPTDPLSLKPEVKEAR

Specific function: Catalyzes the irreversible reduction of 2,3-butanediol to (S)-acetoin in the presence of NADH [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI142976729, Length=193, Percent_Identity=35.7512953367876, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI31542939, Length=200, Percent_Identity=31, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI25282469, Length=285, Percent_Identity=28.4210526315789, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI40807363, Length=195, Percent_Identity=31.7948717948718, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI210032110, Length=198, Percent_Identity=29.7979797979798, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI310128830, Length=201, Percent_Identity=30.3482587064677, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI310115468, Length=201, Percent_Identity=30.3482587064677, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI20149619, Length=195, Percent_Identity=29.2307692307692, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI109715829, Length=208, Percent_Identity=29.3269230769231, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI224922801, Length=169, Percent_Identity=30.1775147928994, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4504505, Length=220, Percent_Identity=28.1818181818182, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI66933014, Length=272, Percent_Identity=26.4705882352941, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI33667109, Length=196, Percent_Identity=29.0816326530612, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI7706318, Length=201, Percent_Identity=26.865671641791, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI15277342, Length=194, Percent_Identity=27.8350515463918, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI5031737, Length=188, Percent_Identity=29.7872340425532, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI31543615, Length=207, Percent_Identity=29.4685990338164, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI87082100, Length=207, Percent_Identity=31.4009661835749, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787905, Length=128, Percent_Identity=34.375, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1787335, Length=199, Percent_Identity=26.1306532663317, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87082160, Length=206, Percent_Identity=28.1553398058252, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789208, Length=207, Percent_Identity=26.0869565217391, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI193203117, Length=197, Percent_Identity=32.48730964467, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI193203115, Length=192, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17508895, Length=193, Percent_Identity=30.5699481865285, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17564282, Length=203, Percent_Identity=29.5566502463054, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17567345, Length=211, Percent_Identity=31.7535545023697, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17568967, Length=221, Percent_Identity=28.5067873303167, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17561402, Length=169, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538486, Length=208, Percent_Identity=29.3269230769231, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25147288, Length=194, Percent_Identity=29.3814432989691, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17555706, Length=195, Percent_Identity=27.6923076923077, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17507613, Length=265, Percent_Identity=24.1509433962264, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI32563809, Length=265, Percent_Identity=24.1509433962264, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6322861, Length=272, Percent_Identity=28.6764705882353, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323882, Length=196, Percent_Identity=31.1224489795918, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24581726, Length=235, Percent_Identity=31.4893617021277, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18859837, Length=222, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24640492, Length=202, Percent_Identity=29.2079207920792, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281364892, Length=203, Percent_Identity=31.5270935960591, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI221510726, Length=225, Percent_Identity=32, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24639444, Length=209, Percent_Identity=32.5358851674641, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648944, Length=202, Percent_Identity=30.6930693069307, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI116007236, Length=208, Percent_Identity=31.25, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571526, Length=179, Percent_Identity=32.4022346368715, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI116007238, Length=200, Percent_Identity=31, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21355631, Length=201, Percent_Identity=27.8606965174129, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17737361, Length=208, Percent_Identity=31.7307692307692, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24644339, Length=190, Percent_Identity=31.0526315789474, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21355319, Length=199, Percent_Identity=29.6482412060301, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571387, Length=213, Percent_Identity=28.6384976525822, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357041, Length=179, Percent_Identity=30.7262569832402, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24641232, Length=222, Percent_Identity=27.4774774774775, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364494, Length=207, Percent_Identity=29.4685990338164, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24641388, Length=217, Percent_Identity=31.7972350230415, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24584752, Length=204, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI116007234, Length=226, Percent_Identity=26.5486725663717, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014007
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.304 [H]

Molecular weight: Translated: 30537; Mature: 30537

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEFDGKVAVITGAGSGFGRAFAEKGASLGMKLVLADVDEGALARTVDTLRAAGAEVIGV
CCEECCCEEEEEECCCCHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEE
RTDVSNGAQVQALADAALEAFGKVHLLFNNAGVGAGGFLWESSANDWAWVFGVNVMGVAH
EECCCCCCCHHHHHHHHHHHHCEEEEEEECCCCCCCCEEEECCCCCEEEEEECHHHHHHH
GVRVFAPIMLGQNEAAHIVNTASVAGLLSPPSMGIYNASKHAVVSLTETLYHDLRNAGGE
HHHHHHHHHCCCCCCHHEEEHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCC
VGCSLLCPAFVPTGIADAERVRPEALRNEAQPTRSQLAADRQLQRAVRSGKLGATDVATL
CCHHEECHHHCCCCCCCHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
TFEAIAERRFYILTHPAILATVRLRHEDIELQRNPTDPLSLKPEVKEAR
HHHHHHCCEEEEEECHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFEFDGKVAVITGAGSGFGRAFAEKGASLGMKLVLADVDEGALARTVDTLRAAGAEVIGV
CCEECCCEEEEEECCCCHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEE
RTDVSNGAQVQALADAALEAFGKVHLLFNNAGVGAGGFLWESSANDWAWVFGVNVMGVAH
EECCCCCCCHHHHHHHHHHHHCEEEEEEECCCCCCCCEEEECCCCCEEEEEECHHHHHHH
GVRVFAPIMLGQNEAAHIVNTASVAGLLSPPSMGIYNASKHAVVSLTETLYHDLRNAGGE
HHHHHHHHHCCCCCCHHEEEHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCC
VGCSLLCPAFVPTGIADAERVRPEALRNEAQPTRSQLAADRQLQRAVRSGKLGATDVATL
CCHHEECHHHCCCCCCCHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
TFEAIAERRFYILTHPAILATVRLRHEDIELQRNPTDPLSLKPEVKEAR
HHHHHHCCEEEEEECHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA