Click here to switch to the map view.

The map label for this gene is aes [H]

Identifier: 186475804

GI number: 186475804

Start: 1168521

End: 1169480

Strand: Direct

Name: aes [H]

Synonym: Bphy_1039

Alternate gene names: 186475804

Gene position: 1168521-1169480 (Clockwise)

Preceding gene: 186475803

Following gene: 186475807

Centisome position: 33.59

GC content: 67.92

Gene sequence:

>960_bases
ATGCCGTTGAACCCGAAGATCGAGCAGGTGCTCGAGATGATTGCGCGTGCGAAGCGTCCCGCGTATCACGATCTGACGCC
GCAAGCCGCGCGCGCGTCGTACGAGAAGAGCGCGCCGATTCTCGAAGTGCCCGCGGCGCCGATGTACTCCATCGAGGACG
TGGCGGTGCCGATGTCCGACGGCGCGGCCGTCCGCGTGCGTCTCTATCATCCTGCCGAGCCGCAATGGGCGAATCTGGCG
CCCGCGCTTGTCTACTATCACGGCGGCGGATTCACGGTCGGCAGCGTGAACACGCACGACGCCATCTGCCGGATGTTCGC
GCGTGACGCGCAGTGCGTCGTGATGTCCGTCGACTACCGCCTCGCACCGGAACACCCGTTTCCCACCGCCGTCGACGACG
CGTTCGACGCTCTGAAGTGGCTGCACGAAAACGCCGCGCCGTACGGCATCGACGCGGCGCGGATCGCCGTCGGCGGCGAC
AGCGCGGGCGGCACGCTCGCCACGGTGTGCGCCGTGCTCGCCCGCGACGCGGGCATCCCGCTCGCGCTCCAACTGCTGAT
CTATCCGGGCACGACGGGCCATCAGCAGACCGACTCGCACGAACGGCTGTCAGACGGCTATCTGCTGTCCGGCGACACGA
TTCAATGGTTCTTCGAGCAATACGTGCGCGACGCCGACGACCGCCACGACTGGCGCTTCGCGCCGCTCGACGGCACGCGC
GGCGCACCGGATTTTCGCGGCGTCGCGCCGGCATGGATCGCGACGGCCGAATACGACCCGCTGAGCGATGAGGGCGAAGC
GTACGCGCTCAAGCTGCGCGAAGCGGGCAACGCCGTCGCGTTCACCTGCTACGCGGGCATGATCCACGAGTTCTTCAAAA
TGGGCGGCTACGTGCCCGACGTGGCGCGCGCGCATGCCGATGCGGCAGCGGCGTTGCGTGAGGCGTTCGGTATCGAGTGA

Upstream 100 bases:

>100_bases
AAGGAAGCCCGCTAACGGTTATCGCGAGCGCGCCGGGCTATGTTCGTGGCATCATGTCGCGCTCATTCAATGTCCGGCGC
TGGCGCGCCATCGTCCGATC

Downstream 100 bases:

>100_bases
GCCGCGAATGAGCCGGCTCGCGGTCAGGCGATGTCGAAGGTGAAGCTGCGCTCGTAATCGCCGGGGCGTTTGACGCGATG
CGAGCCGTTGTCGTCGCTGC

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MPLNPKIEQVLEMIARAKRPAYHDLTPQAARASYEKSAPILEVPAAPMYSIEDVAVPMSDGAAVRVRLYHPAEPQWANLA
PALVYYHGGGFTVGSVNTHDAICRMFARDAQCVVMSVDYRLAPEHPFPTAVDDAFDALKWLHENAAPYGIDAARIAVGGD
SAGGTLATVCAVLARDAGIPLALQLLIYPGTTGHQQTDSHERLSDGYLLSGDTIQWFFEQYVRDADDRHDWRFAPLDGTR
GAPDFRGVAPAWIATAEYDPLSDEGEAYALKLREAGNAVAFTCYAGMIHEFFKMGGYVPDVARAHADAAAALREAFGIE

Sequences:

>Translated_319_residues
MPLNPKIEQVLEMIARAKRPAYHDLTPQAARASYEKSAPILEVPAAPMYSIEDVAVPMSDGAAVRVRLYHPAEPQWANLA
PALVYYHGGGFTVGSVNTHDAICRMFARDAQCVVMSVDYRLAPEHPFPTAVDDAFDALKWLHENAAPYGIDAARIAVGGD
SAGGTLATVCAVLARDAGIPLALQLLIYPGTTGHQQTDSHERLSDGYLLSGDTIQWFFEQYVRDADDRHDWRFAPLDGTR
GAPDFRGVAPAWIATAEYDPLSDEGEAYALKLREAGNAVAFTCYAGMIHEFFKMGGYVPDVARAHADAAAALREAFGIE
>Mature_318_residues
PLNPKIEQVLEMIARAKRPAYHDLTPQAARASYEKSAPILEVPAAPMYSIEDVAVPMSDGAAVRVRLYHPAEPQWANLAP
ALVYYHGGGFTVGSVNTHDAICRMFARDAQCVVMSVDYRLAPEHPFPTAVDDAFDALKWLHENAAPYGIDAARIAVGGDS
AGGTLATVCAVLARDAGIPLALQLLIYPGTTGHQQTDSHERLSDGYLLSGDTIQWFFEQYVRDADDRHDWRFAPLDGTRG
APDFRGVAPAWIATAEYDPLSDEGEAYALKLREAGNAVAFTCYAGMIHEFFKMGGYVPDVARAHADAAAALREAFGIE

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=316, Percent_Identity=28.1645569620253, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI157041239, Length=232, Percent_Identity=31.4655172413793, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI206597554, Length=292, Percent_Identity=29.1095890410959, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI68051721, Length=152, Percent_Identity=35.5263157894737, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI21328446, Length=107, Percent_Identity=37.3831775700935, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI61966717, Length=197, Percent_Identity=28.9340101522843, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI226423947, Length=160, Percent_Identity=33.125, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI157041237, Length=189, Percent_Identity=29.6296296296296, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786682, Length=270, Percent_Identity=31.8518518518519, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17567059, Length=280, Percent_Identity=29.6428571428571, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71996133, Length=164, Percent_Identity=35.9756097560976, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17540028, Length=172, Percent_Identity=31.9767441860465, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI72001146, Length=240, Percent_Identity=29.5833333333333, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115533410, Length=84, Percent_Identity=38.0952380952381, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI115533412, Length=84, Percent_Identity=38.0952380952381, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24656084, Length=101, Percent_Identity=37.6237623762376, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24656076, Length=101, Percent_Identity=37.6237623762376, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20130169, Length=101, Percent_Identity=37.6237623762376, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34622; Mature: 34491

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLNPKIEQVLEMIARAKRPAYHDLTPQAARASYEKSAPILEVPAAPMYSIEDVAVPMSD
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCEEEECCC
GAAVRVRLYHPAEPQWANLAPALVYYHGGGFTVGSVNTHDAICRMFARDAQCVVMSVDYR
CCEEEEEEECCCCCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHCCCEEEEEEECCE
LAPEHPFPTAVDDAFDALKWLHENAAPYGIDAARIAVGGDSAGGTLATVCAVLARDAGIP
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCC
LALQLLIYPGTTGHQQTDSHERLSDGYLLSGDTIQWFFEQYVRDADDRHDWRFAPLDGTR
EEEEEEEECCCCCCCCCCCHHHHCCCEEECCCHHHHHHHHHHHCCCCCCCCEEECCCCCC
GAPDFRGVAPAWIATAEYDPLSDEGEAYALKLREAGNAVAFTCYAGMIHEFFKMGGYVPD
CCCCCCCCCCHHEEECCCCCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHCCCCCH
VARAHADAAAALREAFGIE
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLNPKIEQVLEMIARAKRPAYHDLTPQAARASYEKSAPILEVPAAPMYSIEDVAVPMSD
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCEEEECCC
GAAVRVRLYHPAEPQWANLAPALVYYHGGGFTVGSVNTHDAICRMFARDAQCVVMSVDYR
CCEEEEEEECCCCCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHCCCEEEEEEECCE
LAPEHPFPTAVDDAFDALKWLHENAAPYGIDAARIAVGGDSAGGTLATVCAVLARDAGIP
ECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCC
LALQLLIYPGTTGHQQTDSHERLSDGYLLSGDTIQWFFEQYVRDADDRHDWRFAPLDGTR
EEEEEEEECCCCCCCCCCCHHHHCCCEEECCCHHHHHHHHHHHCCCCCCCCEEECCCCCC
GAPDFRGVAPAWIATAEYDPLSDEGEAYALKLREAGNAVAFTCYAGMIHEFFKMGGYVPD
CCCCCCCCCCHHEEECCCCCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHCCCCCH
VARAHADAAAALREAFGIE
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA