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The map label for this gene is cspA [H]

Identifier: 186475442

GI number: 186475442

Start: 762175

End: 762378

Strand: Direct

Name: cspA [H]

Synonym: Bphy_0676

Alternate gene names: 186475442

Gene position: 762175-762378 (Clockwise)

Preceding gene: 186475439

Following gene: 186475443

Centisome position: 21.91

GC content: 58.33

Gene sequence:

>204_bases
ATGGAAACGGGTATCGTCAAATGGTTTAACGATGCTAAAGGCTACGGCTTCATCACGTCCGACACGGGCGGTGAGGATCT
CTTCGCGCACTTCTCGGAGATCCGCGCGGATGGCTTCAAGTCGCTGAAGGAGAACCAGCGCGTGTCGTTCGACGTGAAGG
CTGGCCCGAAGGGCCGGCAGGCTGCGAACATTCAGCCGCTGTAA

Upstream 100 bases:

>100_bases
GACGCTAATAGGTGCGTTGCATATATTTTTGCTTTTCGGACTTTGATCCTATCCAGCATTAATGGCATCGCTGACTTATT
CAAATCGAGGAAAGTGGAAT

Downstream 100 bases:

>100_bases
TGCTGTTGAGCCGTGGCTCCCAGGATAAACCCTCGCATTGCGGGGGTTTTTTATTGGCTGCCGATCGGCGCGTTAATTCC
TGGCGGGCAGTTTCCTGTTA

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 67; Mature: 67

Protein sequence:

>67_residues
METGIVKWFNDAKGYGFITSDTGGEDLFAHFSEIRADGFKSLKENQRVSFDVKAGPKGRQAANIQPL

Sequences:

>Translated_67_residues
METGIVKWFNDAKGYGFITSDTGGEDLFAHFSEIRADGFKSLKENQRVSFDVKAGPKGRQAANIQPL
>Mature_67_residues
METGIVKWFNDAKGYGFITSDTGGEDLFAHFSEIRADGFKSLKENQRVSFDVKAGPKGRQAANIQPL

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=58.2089552238806, Blast_Score=90, Evalue=4e-20,
Organism=Escherichia coli, GI1789979, Length=65, Percent_Identity=58.4615384615385, Blast_Score=82, Evalue=4e-18,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=54.6875, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI2367114, Length=65, Percent_Identity=50.7692307692308, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1787834, Length=65, Percent_Identity=52.3076923076923, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=50, Blast_Score=72, Evalue=5e-15,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=50, Blast_Score=71, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71983225, Length=60, Percent_Identity=50, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71983217, Length=60, Percent_Identity=50, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7393; Mature: 7393

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METGIVKWFNDAKGYGFITSDTGGEDLFAHFSEIRADGFKSLKENQRVSFDVKAGPKGRQ
CCCCCEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
AANIQPL
CCCCCCC
>Mature Secondary Structure
METGIVKWFNDAKGYGFITSDTGGEDLFAHFSEIRADGFKSLKENQRVSFDVKAGPKGRQ
CCCCCEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
AANIQPL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]