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The map label for this gene is greB [H]

Identifier: 186475439

GI number: 186475439

Start: 759177

End: 759749

Strand: Direct

Name: greB [H]

Synonym: Bphy_0673

Alternate gene names: 186475439

Gene position: 759177-759749 (Clockwise)

Preceding gene: 186475438

Following gene: 186475442

Centisome position: 21.82

GC content: 62.13

Gene sequence:

>573_bases
ATGAACAAAGCCTTTGTCAAAGAATCGACTGACGAGAACGACGACGACCTCGAAGCCGCTCAACCCGATATGCCTGCGGG
CGCGAAGAACTACATCACGCCCGCGGGCTACCGGCGTCTGCGCGACGAACTGCTGCATTTGATCGACGAGGCGCGGCCGG
AAGTCGTGAAACTGGTGTCGTGGGCGGCGTCGAATGGCGACCGCTCGGAGAACGGCGATTACATCTACGGCAAGCGACGG
CTGCGCGAGATCGATCGGCGCATCCGCTTTTTGACGAAGCGTATCGACCTGGCCGAGGTGGTGAACAGCAGCCGCCAGGA
GAACACCGATCAGGTGTTCTTCGGCGCGACCGTCGACTACGCGACAGAGGACGGCGAGACCCATACGGTGAGGATCGTCG
GCATCGACGAGGTGAATCTGGACGAGGGGCATGTCAGCTGGATTTCGCCGATCGCCCGCGCGCTGCTGAAGGCGAAGGTC
GGCGATCAGGTCACACTGCACACGCCAGCAGGCCCGCAGCCGATCGACGTGCTCGATGTCCGCTATCCGACGCGAGAAGA
AGAGGACGCGTAA

Upstream 100 bases:

>100_bases
GCAAGGTCTCGGAAAAAGGGAAGGGGCAACCCGAAATTTGTGACAACGGCCGTATCATGTAGGCTGGAGATCCGGATGTC
CGCTTCAACGCTGAACCATC

Downstream 100 bases:

>100_bases
CTCGTCTTCGGGTGTCGCCGGACGCCTCGCCTCGACCGAGACATGTCGGCACAAACGAAAAAGGCGCCGCAAACGGCGCC
TTTTTCATGCTGCGAGTACT

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MNKAFVKESTDENDDDLEAAQPDMPAGAKNYITPAGYRRLRDELLHLIDEARPEVVKLVSWAASNGDRSENGDYIYGKRR
LREIDRRIRFLTKRIDLAEVVNSSRQENTDQVFFGATVDYATEDGETHTVRIVGIDEVNLDEGHVSWISPIARALLKAKV
GDQVTLHTPAGPQPIDVLDVRYPTREEEDA

Sequences:

>Translated_190_residues
MNKAFVKESTDENDDDLEAAQPDMPAGAKNYITPAGYRRLRDELLHLIDEARPEVVKLVSWAASNGDRSENGDYIYGKRR
LREIDRRIRFLTKRIDLAEVVNSSRQENTDQVFFGATVDYATEDGETHTVRIVGIDEVNLDEGHVSWISPIARALLKAKV
GDQVTLHTPAGPQPIDVLDVRYPTREEEDA
>Mature_190_residues
MNKAFVKESTDENDDDLEAAQPDMPAGAKNYITPAGYRRLRDELLHLIDEARPEVVKLVSWAASNGDRSENGDYIYGKRR
LREIDRRIRFLTKRIDLAEVVNSSRQENTDQVFFGATVDYATEDGETHTVRIVGIDEVNLDEGHVSWISPIARALLKAKV
GDQVTLHTPAGPQPIDVLDVRYPTREEEDA

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=151, Percent_Identity=50.9933774834437, Blast_Score=143, Evalue=6e-36,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=33.5526315789474, Blast_Score=84, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 21434; Mature: 21434

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAFVKESTDENDDDLEAAQPDMPAGAKNYITPAGYRRLRDELLHLIDEARPEVVKLVS
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
WAASNGDRSENGDYIYGKRRLREIDRRIRFLTKRIDLAEVVNSSRQENTDQVFFGATVDY
HHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECEEEE
ATEDGETHTVRIVGIDEVNLDEGHVSWISPIARALLKAKVGDQVTLHTPAGPQPIDVLDV
CCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEC
RYPTREEEDA
CCCCCCCCCC
>Mature Secondary Structure
MNKAFVKESTDENDDDLEAAQPDMPAGAKNYITPAGYRRLRDELLHLIDEARPEVVKLVS
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
WAASNGDRSENGDYIYGKRRLREIDRRIRFLTKRIDLAEVVNSSRQENTDQVFFGATVDY
HHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECEEEE
ATEDGETHTVRIVGIDEVNLDEGHVSWISPIARALLKAKVGDQVTLHTPAGPQPIDVLDV
CCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEC
RYPTREEEDA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]