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The map label for this gene is gutB [H]

Identifier: 186475271

GI number: 186475271

Start: 574653

End: 575741

Strand: Direct

Name: gutB [H]

Synonym: Bphy_0503

Alternate gene names: 186475271

Gene position: 574653-575741 (Clockwise)

Preceding gene: 186475270

Following gene: 186475272

Centisome position: 16.52

GC content: 59.5

Gene sequence:

>1089_bases
ATGACTACTCAGGACAACAGCAAAAGCATGACGGCGATCGTGTGTCACGCGCCGAAAGACTATCGCGTCGAACGCGTGAC
GAAGCCACAGGCGCGCGCGCACGAACTCGTGATTCGCATCGCCGCGTGCGGTATCTGTGCCAGCGACTGCAAATGCCATT
CTGGCGCGAAGATGTTCTGGGGCGGACCGAGCCCGTGGGTGAAGGCGCCCGTGATTCCGGGGCATGAGTTCTTTGGCTAC
GTCGAAGAACTCGGTGAAGGGGCCGCCGAGCATTTCGACGTGAAGAAGGGCGACCGCGTGATTGCCGAACAGATCGTGCC
GTGCGGTAAATGCCGCTATTGCAGGTCCGGCCAGTACTGGATGTGCGAAGTGCACAACATCTTCGGCTTCCAGCGTGAAG
TTGCGGACGGCGGTATGGCCGAATACATGCGCATTCCGCCGACGGCGATCGTGCACAAGATTCCCGATGGCATCTCGCTC
GAAGATGCCGCGATCATCGAACCGCTCGCGTGCGCGATTCACACCGTCAATCGCGGCGACATTCAACTGGACGATGTCGT
CGTGATCGCGGGCGCGGGGCCGCTCGGCTTGATGATGACGCAAGTCGCGCACCTGAAGACGCCGAAGAAACTCGTTGTGA
TCGATCTGGTCGACGAGCGTCTCGAACTTGCACGCGAATATGGCGCCGATGTGACGATCAATCCGAAGAACGACGATGCG
CTCGCCATCGTGAAGTCGCTGACGGACAACTATGGCTGCGATGTCTACATCGAAACGACGGGCGTGCCCGCCGGCGTGAA
TCAGGGCATGGACCTGATTCGCAAGCTTGGGCGTTTCGTCGAGTTTTCCGTGTTCGGTGCGGAGACTACAATGGACTGGT
CGATCATCGGCGACCGCAAGGAGCTCGATGTGCGCGGCGCGCATCTCGGCCCTTACTGCTATCCGGTTGCGATCGATTTG
CTGGCGCGCGGACTCGTCACATCGAAAGGCATCGTCACGCACGGTTTTTCGCTCGAAGAATGGGACGAAGCGATCAAGGT
CGCGAATTCGCTCGATTCGATCAAGGTGTTGTTGAAGCCTGCCCGCTAG

Upstream 100 bases:

>100_bases
TCAAGGGCGGAATCATTCTGCTCGCGATCTACATCAGCCGCGAACGCAAGAAGTAACGGTTCCCTTTCCTCTGTTCTCCA
CTTACAAGCAGGGCAATGCA

Downstream 100 bases:

>100_bases
CTGGATAGTTCGTCCGTCATTCGAAAAATGCACAGTGCGCGTCGTGCAGACGCGCACACGGAGACACTATGAATTACGTC
ATCGGCGTCGATATCGGCAC

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MTTQDNSKSMTAIVCHAPKDYRVERVTKPQARAHELVIRIAACGICASDCKCHSGAKMFWGGPSPWVKAPVIPGHEFFGY
VEELGEGAAEHFDVKKGDRVIAEQIVPCGKCRYCRSGQYWMCEVHNIFGFQREVADGGMAEYMRIPPTAIVHKIPDGISL
EDAAIIEPLACAIHTVNRGDIQLDDVVVIAGAGPLGLMMTQVAHLKTPKKLVVIDLVDERLELAREYGADVTINPKNDDA
LAIVKSLTDNYGCDVYIETTGVPAGVNQGMDLIRKLGRFVEFSVFGAETTMDWSIIGDRKELDVRGAHLGPYCYPVAIDL
LARGLVTSKGIVTHGFSLEEWDEAIKVANSLDSIKVLLKPAR

Sequences:

>Translated_362_residues
MTTQDNSKSMTAIVCHAPKDYRVERVTKPQARAHELVIRIAACGICASDCKCHSGAKMFWGGPSPWVKAPVIPGHEFFGY
VEELGEGAAEHFDVKKGDRVIAEQIVPCGKCRYCRSGQYWMCEVHNIFGFQREVADGGMAEYMRIPPTAIVHKIPDGISL
EDAAIIEPLACAIHTVNRGDIQLDDVVVIAGAGPLGLMMTQVAHLKTPKKLVVIDLVDERLELAREYGADVTINPKNDDA
LAIVKSLTDNYGCDVYIETTGVPAGVNQGMDLIRKLGRFVEFSVFGAETTMDWSIIGDRKELDVRGAHLGPYCYPVAIDL
LARGLVTSKGIVTHGFSLEEWDEAIKVANSLDSIKVLLKPAR
>Mature_361_residues
TTQDNSKSMTAIVCHAPKDYRVERVTKPQARAHELVIRIAACGICASDCKCHSGAKMFWGGPSPWVKAPVIPGHEFFGYV
EELGEGAAEHFDVKKGDRVIAEQIVPCGKCRYCRSGQYWMCEVHNIFGFQREVADGGMAEYMRIPPTAIVHKIPDGISLE
DAAIIEPLACAIHTVNRGDIQLDDVVVIAGAGPLGLMMTQVAHLKTPKKLVVIDLVDERLELAREYGADVTINPKNDDAL
AIVKSLTDNYGCDVYIETTGVPAGVNQGMDLIRKLGRFVEFSVFGAETTMDWSIIGDRKELDVRGAHLGPYCYPVAIDLL
ARGLVTSKGIVTHGFSLEEWDEAIKVANSLDSIKVLLKPAR

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=350, Percent_Identity=31.7142857142857, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4501929, Length=395, Percent_Identity=24.8101265822785, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI71565154, Length=251, Percent_Identity=27.0916334661355, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI4501933, Length=279, Percent_Identity=26.8817204301075, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI34577061, Length=279, Percent_Identity=26.5232974910394, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI71743840, Length=271, Percent_Identity=26.9372693726937, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI262073058, Length=271, Percent_Identity=26.9372693726937, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1787863, Length=341, Percent_Identity=29.6187683284457, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788073, Length=359, Percent_Identity=28.4122562674095, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1790045, Length=337, Percent_Identity=31.1572700296736, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1788407, Length=362, Percent_Identity=27.9005524861878, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI226510992, Length=350, Percent_Identity=27.4285714285714, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788075, Length=363, Percent_Identity=28.3746556473829, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1786825, Length=279, Percent_Identity=30.8243727598566, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790718, Length=315, Percent_Identity=27.3015873015873, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87082125, Length=362, Percent_Identity=27.3480662983425, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081918, Length=303, Percent_Identity=30.6930693069307, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562876, Length=363, Percent_Identity=32.5068870523416, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17562878, Length=364, Percent_Identity=32.6923076923077, Blast_Score=157, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI25146526, Length=255, Percent_Identity=26.2745098039216, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71997431, Length=255, Percent_Identity=25.4901960784314, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323099, Length=369, Percent_Identity=28.9972899728997, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6322619, Length=349, Percent_Identity=28.6532951289398, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319955, Length=349, Percent_Identity=28.6532951289398, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320033, Length=281, Percent_Identity=28.1138790035587, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319257, Length=367, Percent_Identity=27.2479564032698, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6319258, Length=274, Percent_Identity=25.5474452554745, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323729, Length=309, Percent_Identity=24.5954692556634, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17137530, Length=346, Percent_Identity=30.0578034682081, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17737897, Length=359, Percent_Identity=29.5264623955432, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI17737895, Length=386, Percent_Identity=25.3886010362694, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI221457811, Length=346, Percent_Identity=23.4104046242775, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550770, Length=346, Percent_Identity=23.4104046242775, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45551930, Length=346, Percent_Identity=23.4104046242775, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 39594; Mature: 39463

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQDNSKSMTAIVCHAPKDYRVERVTKPQARAHELVIRIAACGICASDCKCHSGAKMFW
CCCCCCCCCEEEEEEECCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
GGPSPWVKAPVIPGHEFFGYVEELGEGAAEHFDVKKGDRVIAEQIVPCGKCRYCRSGQYW
CCCCCCEECCCCCCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCEE
MCEVHNIFGFQREVADGGMAEYMRIPPTAIVHKIPDGISLEDAAIIEPLACAIHTVNRGD
EEEEHHHHCHHHHHHCCCHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHEECCCCC
IQLDDVVVIAGAGPLGLMMTQVAHLKTPKKLVVIDLVDERLELAREYGADVTINPKNDDA
EEECCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCCEEECCCCCCH
LAIVKSLTDNYGCDVYIETTGVPAGVNQGMDLIRKLGRFVEFSVFGAETTMDWSIIGDRK
HHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCH
ELDVRGAHLGPYCYPVAIDLLARGLVTSKGIVTHGFSLEEWDEAIKVANSLDSIKVLLKP
HCEECCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHEEEEEEC
AR
CC
>Mature Secondary Structure 
TTQDNSKSMTAIVCHAPKDYRVERVTKPQARAHELVIRIAACGICASDCKCHSGAKMFW
CCCCCCCCEEEEEEECCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
GGPSPWVKAPVIPGHEFFGYVEELGEGAAEHFDVKKGDRVIAEQIVPCGKCRYCRSGQYW
CCCCCCEECCCCCCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCEE
MCEVHNIFGFQREVADGGMAEYMRIPPTAIVHKIPDGISLEDAAIIEPLACAIHTVNRGD
EEEEHHHHCHHHHHHCCCHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHEECCCCC
IQLDDVVVIAGAGPLGLMMTQVAHLKTPKKLVVIDLVDERLELAREYGADVTINPKNDDA
EEECCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCCEEECCCCCCH
LAIVKSLTDNYGCDVYIETTGVPAGVNQGMDLIRKLGRFVEFSVFGAETTMDWSIIGDRK
HHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCH
ELDVRGAHLGPYCYPVAIDLLARGLVTSKGIVTHGFSLEEWDEAIKVANSLDSIKVLLKP
HCEECCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHEEEEEEC
AR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]