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The map label for this gene is purU [H]

Identifier: 186475105

GI number: 186475105

Start: 377293

End: 378156

Strand: Direct

Name: purU [H]

Synonym: Bphy_0336

Alternate gene names: 186475105

Gene position: 377293-378156 (Clockwise)

Preceding gene: 186475104

Following gene: 186475109

Centisome position: 10.84

GC content: 60.19

Gene sequence:

>864_bases
ATGTCGACCGAACACAGCTTTATTCTCAAACTGTCGTGCGCCGACCGGCCCGGCATCGTCCACGCCGTTTCGGGTTTCCT
GTTCGAGCGTGGCAGCAACATCCTCGACTCCGCCCAATTCGGCGACAGCCATACGGGCGAATTTTTCATGCGCGTTCACT
TCCAGCAGGTGGGCGGCGATCCGGGTCTCGACGCGCTCCGCGAAGCGTTTGCAACGCTCGCCGAGCAGTTCGGCATGCGC
TGGGAACTGCACGATGCGAACGTGAAGCCGCGCGTCGTGATCATGGTCTCGAAGATCGGCCACTGTTTAAACGATCTGCT
GTTCCGCTATCGCACCGGGCAGCTGAACATCGAGATTCCCGCCATCATCTCGAACCACAAGGAGTTCTATCAGCTCGCCG
CGAGCTACGACATTCCGTTCCATCACTTTCCGCTGACGTCGAGCGATACGAAAGCGCATCAGGAAGCGCGCGTGCTTGAA
GTGATCGACGAATGCAAGGCTGACCTCGTGGTGCTCGCGCGCTATATGCAGATTCTGTCGCCGCAGTTGTGTGCACGGTT
GGCCGGGCGTGCGATCAACATCCACCATTCGTTCCTGCCCAGCTTCAAGGGCGCGAAGCCGTATTACCAGGCATTCGATC
GCGGCGTGAAACTGATCGGTGCGACGGCGCACTATGTGACGACGGATCTCGACGAAGGCCCGATCATCGAGCAGGAAGTG
GAGCGCGTCGATCACAGCATGACGCCGGATCAACTGACGGCGATCGGCCGCGACGTCGAATGCGTGACGCTGGCGCGTGC
GGTGAAGTGGCACGTCGAGCATCGGATCGTGCTGAACGGCAGCAAGACGGTCGTGTTCCGCTGA

Upstream 100 bases:

>100_bases
CTGTTCGAGCCGCCCGTTCTCGATTGATCTGAGGCACCGTCGCAGACGTGCCGCAATACCTATCAAGCAACACGCACTGA
AGAAGGGAGTCACCGAGGTC

Downstream 100 bases:

>100_bases
TCGGTTTTTATTTCGTCTGAACCGGCGCGTGCCTTGCTCGGCCGCGCCGGTTTTTTGGGGTGAATGAGCGGCAGCGTTAC
CGTGAAGCGGCGCTTTCCGT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSTEHSFILKLSCADRPGIVHAVSGFLFERGSNILDSAQFGDSHTGEFFMRVHFQQVGGDPGLDALREAFATLAEQFGMR
WELHDANVKPRVVIMVSKIGHCLNDLLFRYRTGQLNIEIPAIISNHKEFYQLAASYDIPFHHFPLTSSDTKAHQEARVLE
VIDECKADLVVLARYMQILSPQLCARLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGPIIEQEV
ERVDHSMTPDQLTAIGRDVECVTLARAVKWHVEHRIVLNGSKTVVFR

Sequences:

>Translated_287_residues
MSTEHSFILKLSCADRPGIVHAVSGFLFERGSNILDSAQFGDSHTGEFFMRVHFQQVGGDPGLDALREAFATLAEQFGMR
WELHDANVKPRVVIMVSKIGHCLNDLLFRYRTGQLNIEIPAIISNHKEFYQLAASYDIPFHHFPLTSSDTKAHQEARVLE
VIDECKADLVVLARYMQILSPQLCARLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGPIIEQEV
ERVDHSMTPDQLTAIGRDVECVTLARAVKWHVEHRIVLNGSKTVVFR
>Mature_286_residues
STEHSFILKLSCADRPGIVHAVSGFLFERGSNILDSAQFGDSHTGEFFMRVHFQQVGGDPGLDALREAFATLAEQFGMRW
ELHDANVKPRVVIMVSKIGHCLNDLLFRYRTGQLNIEIPAIISNHKEFYQLAASYDIPFHHFPLTSSDTKAHQEARVLEV
IDECKADLVVLARYMQILSPQLCARLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGPIIEQEVE
RVDHSMTPDQLTAIGRDVECVTLARAVKWHVEHRIVLNGSKTVVFR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=165, Percent_Identity=33.9393939393939, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI209869995, Length=165, Percent_Identity=33.9393939393939, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI209869993, Length=165, Percent_Identity=33.9393939393939, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1787483, Length=281, Percent_Identity=40.9252669039146, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788846, Length=190, Percent_Identity=28.9473684210526, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=187, Percent_Identity=32.0855614973262, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32280; Mature: 32149

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEHSFILKLSCADRPGIVHAVSGFLFERGSNILDSAQFGDSHTGEFFMRVHFQQVGGD
CCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEEEHHHHCCC
PGLDALREAFATLAEQFGMRWELHDANVKPRVVIMVSKIGHCLNDLLFRYRTGQLNIEIP
CCHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEEC
AIISNHKEFYQLAASYDIPFHHFPLTSSDTKAHQEARVLEVIDECKADLVVLARYMQILS
HHHHCCHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PQLCARLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGPIIEQEV
HHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEEECCCCCCCCHHHH
ERVDHSMTPDQLTAIGRDVECVTLARAVKWHVEHRIVLNGSKTVVFR
HHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHCEEEEEECCEEEEEC
>Mature Secondary Structure 
STEHSFILKLSCADRPGIVHAVSGFLFERGSNILDSAQFGDSHTGEFFMRVHFQQVGGD
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEEEHHHHCCC
PGLDALREAFATLAEQFGMRWELHDANVKPRVVIMVSKIGHCLNDLLFRYRTGQLNIEIP
CCHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEEC
AIISNHKEFYQLAASYDIPFHHFPLTSSDTKAHQEARVLEVIDECKADLVVLARYMQILS
HHHHCCHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PQLCARLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGPIIEQEV
HHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEEECCCCCCCCHHHH
ERVDHSMTPDQLTAIGRDVECVTLARAVKWHVEHRIVLNGSKTVVFR
HHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHCEEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]