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The map label for this gene is metK [H]

Identifier: 186474849

GI number: 186474849

Start: 88106

End: 89293

Strand: Direct

Name: metK [H]

Synonym: Bphy_0078

Alternate gene names: 186474849

Gene position: 88106-89293 (Clockwise)

Preceding gene: 186474839

Following gene: 186474851

Centisome position: 2.53

GC content: 63.64

Gene sequence:

>1188_bases
GTGGCTAACGATTACCTCTTCACCTCCGAATCCGTCTCCGAAGGCCATCCGGACAAGGTCGCAGACCAGATTTCGGACGC
TATCCTCGACGCGATCCTCGCCCAGGACAAATACTCGCGTGTTGCGGCCGAAACGCTGTGCAACACGGGTCTCGTCGTGC
TGGCGGGTGAAATCACCACGACGGCAAACGTCGATTACATCCAGGTCGCGCGCGAAACCATCAAGCGCATCGGCTATGAC
AACACCGACTACGGCATCGACTATCGCGGCTGCGCGGTGCTCGTCGCGTACGACAAGCAATCGCCGGACATCGCGCAAGG
CGTCGACCGTGCGCACGACAACAATCTCGACCAGGGCGCCGGCGACCAGGGCCTGATGTTCGGCTATGCATGCGACGAAA
CGCCCGAACTGATGCCGCTGCCGATCCACCTGTCGCACCGCCTCGTCGAGCGTCAGGCGAATCTGCGCCGCGACGGCCGT
CTGCCCTGGTTGCGTCCGGATGCGAAGTCGCAGGTCACGATTCGCTACGTCGACGGCAAGCCGCACTCGATCGACACCGT
CGTGCTGTCCACGCAGCACTCACCGGATATCGACCTCGGACAACTGCGCGAAGCCGTGATCGAAGAAATCATCAAGCCGA
CGCTGCCCGCCGAACTGATCAAGGGCGACATCAAGTTCCTCGTGAACCCGACGGGCCGGTTCGTGATCGGCGGCCCGCAG
GGCGATTGCGGTCTGACGGGCCGCAAAATTATCGTCGATACGTACGGCGGCGCGGCTCCGCACGGCGGCGGCGCGTTCTC
GGGCAAGGATCCTTCGAAGGTCGACCGCTCGGCGGCGTATGCCGGCCGCTATGTTGCAAAGAACATCGTCGCAGCCGGCC
TCGCATCGCGCGCGCTGATCCAGGTGTCGTACGCAATCGGCGTTGCACAGCCGACCTCGGTGATGGTCAACACGTTCGGC
ACGGGCCGCGTGTCGGATGCGACGATCACACGTCTGGTTCAGGAACACTTCGACCTGCGTCCGAAGGGCATCATCCAGAT
GCTGGATCTGTTGCGCCCAGTCTATGAAAAAACGGCCGCCTACGGCCACTTCGGCCGCGAGGAGCCGGAATTCTCGTGGG
AAGCGACCGACAAGGCGCTCGCGCTGGCCGAAGCGGCTGGCACGGAGCCGGTTGCCGCTCTCGCCTGA

Upstream 100 bases:

>100_bases
GATAGTCGCCCGGCGGTAAGGAATTCCGCTAAAGCGTCGCCGCTTCAGGCTCGAAGCAGGCTACGTGAAACTCAACCGTA
ACCAACAAGGAGCCTGTAAC

Downstream 100 bases:

>100_bases
ACGACGCTGTGAGCGATACGCGCGGCCGAATGTCGCGCGGCATCGATCTCCAGGCCGCAAAGAAAAACGCCCTGCATGCG
AACGCATGCGGGGCGTTTTT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITTTANVDYIQVARETIKRIGYD
NTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGAGDQGLMFGYACDETPELMPLPIHLSHRLVERQANLRRDGR
LPWLRPDAKSQVTIRYVDGKPHSIDTVVLSTQHSPDIDLGQLREAVIEEIIKPTLPAELIKGDIKFLVNPTGRFVIGGPQ
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAQPTSVMVNTFG
TGRVSDATITRLVQEHFDLRPKGIIQMLDLLRPVYEKTAAYGHFGREEPEFSWEATDKALALAEAAGTEPVAALA

Sequences:

>Translated_395_residues
MANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITTTANVDYIQVARETIKRIGYD
NTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGAGDQGLMFGYACDETPELMPLPIHLSHRLVERQANLRRDGR
LPWLRPDAKSQVTIRYVDGKPHSIDTVVLSTQHSPDIDLGQLREAVIEEIIKPTLPAELIKGDIKFLVNPTGRFVIGGPQ
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAQPTSVMVNTFG
TGRVSDATITRLVQEHFDLRPKGIIQMLDLLRPVYEKTAAYGHFGREEPEFSWEATDKALALAEAAGTEPVAALA
>Mature_394_residues
ANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITTTANVDYIQVARETIKRIGYDN
TDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGAGDQGLMFGYACDETPELMPLPIHLSHRLVERQANLRRDGRL
PWLRPDAKSQVTIRYVDGKPHSIDTVVLSTQHSPDIDLGQLREAVIEEIIKPTLPAELIKGDIKFLVNPTGRFVIGGPQG
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAQPTSVMVNTFGT
GRVSDATITRLVQEHFDLRPKGIIQMLDLLRPVYEKTAAYGHFGREEPEFSWEATDKALALAEAAGTEPVAALA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=58.1578947368421, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=57.1052631578947, Blast_Score=422, Evalue=1e-118,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=69.9738903394256, Blast_Score=535, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI17538498, Length=376, Percent_Identity=59.0425531914894, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145633, Length=380, Percent_Identity=58.6842105263158, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=58.5106382978723, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17551082, Length=401, Percent_Identity=55.6109725685786, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32565905, Length=376, Percent_Identity=52.9255319148936, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565911, Length=328, Percent_Identity=57.6219512195122, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI25145635, Length=332, Percent_Identity=56.9277108433735, Blast_Score=356, Evalue=9e-99,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=57.1428571428571, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=59.5238095238095, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320710, Length=379, Percent_Identity=56.7282321899736, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=57.1428571428571, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI45552159, Length=376, Percent_Identity=54.7872340425532, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580529, Length=376, Percent_Identity=54.7872340425532, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580537, Length=376, Percent_Identity=54.7872340425532, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580533, Length=376, Percent_Identity=54.7872340425532, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580535, Length=376, Percent_Identity=54.7872340425532, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580531, Length=376, Percent_Identity=54.7872340425532, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580527, Length=374, Percent_Identity=54.2780748663102, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580525, Length=374, Percent_Identity=54.2780748663102, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580541, Length=375, Percent_Identity=52, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=56.3025210084034, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42605; Mature: 42473

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANVDYIQVARETIKRIGYDNTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCC
GDQGLMFGYACDETPELMPLPIHLSHRLVERQANLRRDGRLPWLRPDAKSQVTIRYVDGK
CCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PHSIDTVVLSTQHSPDIDLGQLREAVIEEIIKPTLPAELIKGDIKFLVNPTGRFVIGGPQ
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCEEEECCC
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
QVSYAIGVAQPTSVMVNTFGTGRVSDATITRLVQEHFDLRPKGIIQMLDLLRPVYEKTAA
EEHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YGHFGREEPEFSWEATDKALALAEAAGTEPVAALA
HCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHCC
>Mature Secondary Structure 
ANDYLFTSESVSEGHPDKVADQISDAILDAILAQDKYSRVAAETLCNTGLVVLAGEITT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEE
TANVDYIQVARETIKRIGYDNTDYGIDYRGCAVLVAYDKQSPDIAQGVDRAHDNNLDQGA
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCC
GDQGLMFGYACDETPELMPLPIHLSHRLVERQANLRRDGRLPWLRPDAKSQVTIRYVDGK
CCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PHSIDTVVLSTQHSPDIDLGQLREAVIEEIIKPTLPAELIKGDIKFLVNPTGRFVIGGPQ
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCCEEEECCC
GDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
QVSYAIGVAQPTSVMVNTFGTGRVSDATITRLVQEHFDLRPKGIIQMLDLLRPVYEKTAA
EEHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YGHFGREEPEFSWEATDKALALAEAAGTEPVAALA
HCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA