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The map label for this gene is pckG

Identifier: 186474801

GI number: 186474801

Start: 33920

End: 35776

Strand: Direct

Name: pckG

Synonym: Bphy_0030

Alternate gene names: 186474801

Gene position: 33920-35776 (Clockwise)

Preceding gene: 186474799

Following gene: 186474802

Centisome position: 0.97

GC content: 64.35

Gene sequence:

>1857_bases
ATGAATCATCCCGCATTTGCAGCCCATTCAACCCTTGAAGCGCCTGCCTGGGTCAAACACAAAAAGCTGATCGAGTGGGT
GCAGCGCGTTGCGGCGCTGGCGAAGCCGGAGCGGATCGTCTGGTGCGACGGTTCGCAGGAGGAATATGACCGCCTGACGG
CGCAGATGGTCGCGGCCGGCACCCTCAGAAGGCTCAACCCGGCCAAACGGCCGAACTCGTATCTCGCCTGTTCCGATCCG
TCCGACGTCGCGCGCGTCGAGGACCGTACGTTCATCTGTTCGCAGCGTCGTGAAGACGCTGGCCCGACCAACAACTGGAT
CGCGCCCGCCGAGATGCGCGCAACGCTCGACGGTCTGTTCGACGGCGCGATGCGCGGCCGTACGATGTACGTCGTGCCAT
TCTCGATGGGGCCGCTCGGCTCGCCGATCGCCCATATAGGCGTCGAGCTGAGCGATAGCCCATACGTCGTCAGCAACATG
CGGATCATGACGCGCATGGGCCGCAAGGTGTACGACGTACTCGGCGAGGACGGCGAGTTCGTGCCGTGCGTGCATTCCGT
CGGCGCGCCGCTCGCGGCAGGCCAGAAGGACGTGGCCTGGCCGTGTAACGACACGAAATACATCGTCCACTTCCCGGAAT
CGCGTGAAATCTGGAGCTATGGCTCGGGCTACGGCGGCAATGCGCTGCTCGGCAAGAAGTGCTTCGCGCTGCGTATCGCG
TCCACGATGGGCCGCGCCGAAGGCTGGCTCGCCGAACACATGCTGGTGCTCGGCGTGACCTCGCCCGAAGGCAAGAAGCA
CCATGTGGCGGCGGCTTTCCCGTCGGCCTGCGGCAAGACCAATTTCGCGATGCTGATTCCGCCGCAAGGCATGAACGGCT
GGAAGATCACGACGATCGGCGACGACATCGCCTGGATCAAGCCAGGCAAGGACGGGCGTCTTTACGCGATCAACCCGGAA
GCGGGCTATTTCGGCGTCGCGCCGGGCACGAGCGAAAAGACCAACTTCAACGCGATGGCCACGCTGAAGGAAAACGTGAT
CTTCACGAACGTCGCGCTTACGGACGACGGCGACGTCTGGTGGGAGGGCATGACGGACGAGCCGCCCGCGCATCTGATCG
ACTGGCAGGGCAGGGACTGGACGCCCGCCATCGCGAAAGAAACGGGCCGCAAGGCGGCGCATCCGAACGCCCGCTTCACG
GCGCCCGCTTCGCAATGCCCGTCGATCGACGCCGACTGGGAAAACCCAGCAGGCGTGCCGATCGACGCGTTCGTGTTCGG
CGGCCGTCGTTCGACGACCGTGCCGCTCGTGACGGAAGCGCGCGACTGGGTCGAAGGCGTCTACATGGCCGCGACGATGG
GCTCGGAAACGACGGCGGCGGCGGCCGGACAGCAGGGCGTCGTGCGGCGCGATCCGTTCGCGATGCTGCCGTTCTGCGGC
TACAACATGAGCGACTACTTTGCCCACTGGCTGAAGACGGGCGTGAAACTGGAGCAGATGAGCGTGCGCACGCCCAAGAT
TTTCTGCGTCAACTGGTTCCGCAAGGGCGACGACGGCAAGTTCGTGTGGCCTGGCTTCGGCGAAAACATGCGCGTGCTGC
GCTGGATGGTCGATCGGATCGAGGGTAAGGCGAAGGGAGAGGAGCATGCGTTCGGCGTGTCCCCGCGCTATGACGACCTC
GACTGGAGCGGGCTGGACTTCACGCGCGAGCAATTCGAACAGGTGATTTCGGTCGATGATGCCGCCTGGCGCAAGGAACT
CGCGTTGCACAGCGAGTTGTTCGACACGCTGAAGCAGGGCCTGCCTGTCGCACTGCAAGACACCCGCAGCGCGCTCGAAA
AGAAGCTCACCGCGTGA

Upstream 100 bases:

>100_bases
TCGCGCGTCCGGGAACGGTTCGCGCAGAGTGTCGCCTCTCGCACGCAGCGTCGCTGGGCCAGCACCGTTTTCATACTTCT
ACCAAAACGGAGACAGCTCG

Downstream 100 bases:

>100_bases
CATTGTGAGCGCTCACTAACGCCCATATGAGCCGCCCTCGGGCGGCTTTCTTGAGCGCGTCCGACCGGTTTCGCTCGCAT
TCCGCCCCATTTCCCCGCCG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MNHPAFAAHSTLEAPAWVKHKKLIEWVQRVAALAKPERIVWCDGSQEEYDRLTAQMVAAGTLRRLNPAKRPNSYLACSDP
SDVARVEDRTFICSQRREDAGPTNNWIAPAEMRATLDGLFDGAMRGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVVSNM
RIMTRMGRKVYDVLGEDGEFVPCVHSVGAPLAAGQKDVAWPCNDTKYIVHFPESREIWSYGSGYGGNALLGKKCFALRIA
STMGRAEGWLAEHMLVLGVTSPEGKKHHVAAAFPSACGKTNFAMLIPPQGMNGWKITTIGDDIAWIKPGKDGRLYAINPE
AGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMTDEPPAHLIDWQGRDWTPAIAKETGRKAAHPNARFT
APASQCPSIDADWENPAGVPIDAFVFGGRRSTTVPLVTEARDWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
YNMSDYFAHWLKTGVKLEQMSVRTPKIFCVNWFRKGDDGKFVWPGFGENMRVLRWMVDRIEGKAKGEEHAFGVSPRYDDL
DWSGLDFTREQFEQVISVDDAAWRKELALHSELFDTLKQGLPVALQDTRSALEKKLTA

Sequences:

>Translated_618_residues
MNHPAFAAHSTLEAPAWVKHKKLIEWVQRVAALAKPERIVWCDGSQEEYDRLTAQMVAAGTLRRLNPAKRPNSYLACSDP
SDVARVEDRTFICSQRREDAGPTNNWIAPAEMRATLDGLFDGAMRGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVVSNM
RIMTRMGRKVYDVLGEDGEFVPCVHSVGAPLAAGQKDVAWPCNDTKYIVHFPESREIWSYGSGYGGNALLGKKCFALRIA
STMGRAEGWLAEHMLVLGVTSPEGKKHHVAAAFPSACGKTNFAMLIPPQGMNGWKITTIGDDIAWIKPGKDGRLYAINPE
AGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMTDEPPAHLIDWQGRDWTPAIAKETGRKAAHPNARFT
APASQCPSIDADWENPAGVPIDAFVFGGRRSTTVPLVTEARDWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
YNMSDYFAHWLKTGVKLEQMSVRTPKIFCVNWFRKGDDGKFVWPGFGENMRVLRWMVDRIEGKAKGEEHAFGVSPRYDDL
DWSGLDFTREQFEQVISVDDAAWRKELALHSELFDTLKQGLPVALQDTRSALEKKLTA
>Mature_618_residues
MNHPAFAAHSTLEAPAWVKHKKLIEWVQRVAALAKPERIVWCDGSQEEYDRLTAQMVAAGTLRRLNPAKRPNSYLACSDP
SDVARVEDRTFICSQRREDAGPTNNWIAPAEMRATLDGLFDGAMRGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVVSNM
RIMTRMGRKVYDVLGEDGEFVPCVHSVGAPLAAGQKDVAWPCNDTKYIVHFPESREIWSYGSGYGGNALLGKKCFALRIA
STMGRAEGWLAEHMLVLGVTSPEGKKHHVAAAFPSACGKTNFAMLIPPQGMNGWKITTIGDDIAWIKPGKDGRLYAINPE
AGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMTDEPPAHLIDWQGRDWTPAIAKETGRKAAHPNARFT
APASQCPSIDADWENPAGVPIDAFVFGGRRSTTVPLVTEARDWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
YNMSDYFAHWLKTGVKLEQMSVRTPKIFCVNWFRKGDDGKFVWPGFGENMRVLRWMVDRIEGKAKGEEHAFGVSPRYDDL
DWSGLDFTREQFEQVISVDDAAWRKELALHSELFDTLKQGLPVALQDTRSALEKKLTA

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI187281517, Length=607, Percent_Identity=50.9060955518946, Blast_Score=603, Evalue=1e-172,
Organism=Homo sapiens, GI66346721, Length=607, Percent_Identity=50.0823723228995, Blast_Score=572, Evalue=1e-163,
Organism=Homo sapiens, GI66346723, Length=378, Percent_Identity=53.1746031746032, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17555492, Length=623, Percent_Identity=49.2776886035313, Blast_Score=561, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI71989645, Length=611, Percent_Identity=47.1358428805237, Blast_Score=550, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI71989658, Length=611, Percent_Identity=47.1358428805237, Blast_Score=549, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI32564411, Length=565, Percent_Identity=52.5663716814159, Blast_Score=546, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI71989653, Length=598, Percent_Identity=47.6588628762542, Blast_Score=545, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI32564407, Length=531, Percent_Identity=51.9774011299435, Blast_Score=515, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI71989649, Length=536, Percent_Identity=48.3208955223881, Blast_Score=491, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI32564409, Length=377, Percent_Identity=49.0716180371353, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17540956, Length=523, Percent_Identity=26.1950286806883, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI24655085, Length=604, Percent_Identity=49.5033112582781, Blast_Score=579, Evalue=1e-165,
Organism=Drosophila melanogaster, GI24655082, Length=606, Percent_Identity=49.1749174917492, Blast_Score=570, Evalue=1e-163,
Organism=Drosophila melanogaster, GI161077226, Length=575, Percent_Identity=50.0869565217391, Blast_Score=547, Evalue=1e-155,
Organism=Drosophila melanogaster, GI24655088, Length=509, Percent_Identity=51.0805500982318, Blast_Score=509, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_BURP8 (B2JJT8)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001856271.1
- ProteinModelPortal:   B2JJT8
- SMR:   B2JJT8
- GeneID:   6241534
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_0030
- HOGENOM:   HBG484437
- OMA:   CGYHMGD
- ProtClustDB:   PRK04210
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 68288; Mature: 68288

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00505 PEPCK_GTP

Important sites: ACT_SITE 277-277 BINDING 85-85 BINDING 226-226 BINDING 233-233 BINDING 275-275 BINDING 398-398 BINDING 429-429

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHPAFAAHSTLEAPAWVKHKKLIEWVQRVAALAKPERIVWCDGSQEEYDRLTAQMVAAG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH
TLRRLNPAKRPNSYLACSDPSDVARVEDRTFICSQRREDAGPTNNWIAPAEMRATLDGLF
HHHHCCCCCCCCCEEEECCCHHHHHHCCCEEEHHCCCCCCCCCCCCCCHHHHHHHHHHHH
DGAMRGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVVSNMRIMTRMGRKVYDVLGEDGEF
HHHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCCE
VPCVHSVGAPLAAGQKDVAWPCNDTKYIVHFPESREIWSYGSGYGGNALLGKKCFALRIA
EHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCHHCCHHHHHHHH
STMGRAEGWLAEHMLVLGVTSPEGKKHHVAAAFPSACGKTNFAMLIPPQGMNGWKITTIG
HHHCHHHHHHHHCEEEEEECCCCCCCEEEEEECCHHCCCCCEEEEECCCCCCCEEEEEEC
DDIAWIKPGKDGRLYAINPEAGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVW
CCEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCEEEEEEEEECCCCEE
WEGMTDEPPAHLIDWQGRDWTPAIAKETGRKAAHPNARFTAPASQCPSIDADWENPAGVP
ECCCCCCCCCCEEECCCCCCCCHHHHHCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCCC
IDAFVFGGRRSTTVPLVTEARDWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
EEEEEECCCCCCEEEEEHHHHHHHHHEEEEEECCCCCHHHHCCCCCCEECCCCEECCCCC
YNMSDYFAHWLKTGVKLEQMSVRTPKIFCVNWFRKGDDGKFVWPGFGENMRVLRWMVDRI
CCHHHHHHHHHHCCCEEEEEECCCCEEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHH
EGKAKGEEHAFGVSPRYDDLDWSGLDFTREQFEQVISVDDAAWRKELALHSELFDTLKQG
CCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
LPVALQDTRSALEKKLTA
CCEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MNHPAFAAHSTLEAPAWVKHKKLIEWVQRVAALAKPERIVWCDGSQEEYDRLTAQMVAAG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH
TLRRLNPAKRPNSYLACSDPSDVARVEDRTFICSQRREDAGPTNNWIAPAEMRATLDGLF
HHHHCCCCCCCCCEEEECCCHHHHHHCCCEEEHHCCCCCCCCCCCCCCHHHHHHHHHHHH
DGAMRGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVVSNMRIMTRMGRKVYDVLGEDGEF
HHHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCCCE
VPCVHSVGAPLAAGQKDVAWPCNDTKYIVHFPESREIWSYGSGYGGNALLGKKCFALRIA
EHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCHHCCHHHHHHHH
STMGRAEGWLAEHMLVLGVTSPEGKKHHVAAAFPSACGKTNFAMLIPPQGMNGWKITTIG
HHHCHHHHHHHHCEEEEEECCCCCCCEEEEEECCHHCCCCCEEEEECCCCCCCEEEEEEC
DDIAWIKPGKDGRLYAINPEAGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVW
CCEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCEEEEEEEEECCCCEE
WEGMTDEPPAHLIDWQGRDWTPAIAKETGRKAAHPNARFTAPASQCPSIDADWENPAGVP
ECCCCCCCCCCEEECCCCCCCCHHHHHCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCCC
IDAFVFGGRRSTTVPLVTEARDWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
EEEEEECCCCCCEEEEEHHHHHHHHHEEEEEECCCCCHHHHCCCCCCEECCCCEECCCCC
YNMSDYFAHWLKTGVKLEQMSVRTPKIFCVNWFRKGDDGKFVWPGFGENMRVLRWMVDRI
CCHHHHHHHHHHCCCEEEEEECCCCEEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHH
EGKAKGEEHAFGVSPRYDDLDWSGLDFTREQFEQVISVDDAAWRKELALHSELFDTLKQG
CCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
LPVALQDTRSALEKKLTA
CCEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA