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The map label for this gene is cysK2 [H]

Identifier: 183222435

GI number: 183222435

Start: 3197443

End: 3198417

Strand: Direct

Name: cysK2 [H]

Synonym: LEPBI_I3089

Alternate gene names: 183222435

Gene position: 3197443-3198417 (Clockwise)

Preceding gene: 183222429

Following gene: 183222436

Centisome position: 88.83

GC content: 43.28

Gene sequence:

>975_bases
ATGCAAACAGAAATTAGAAATGGATTTATTGCGACGATTGGAAATACACCTCTCATCCGCATTCGTTCCTTAAGTGAAGA
AACAGGATGTGAAATCCTGGGAAAGGCGGAATTTTTAAACCCTGGTGGTTCTGTCAAAGACCGCGCAGCTCTTTACATCA
TTGAAGATGCGGAAAAAAGAGGCCTCTTAAAAAAAGGGGGAACCGTTGTGGAAGGGACGGCGGGCAATACAGGAATTGGT
CTCACTCATATTTGTAATGCAAAAGGTTACAAATCCATCATTGTCATCCCCGAAACACAATCCAAAGAAAAAATTGAAAT
GTTACGCACGTTAGGTGCAGAAGTCATCTTAGTACCTGCAGTACCTTATGTTGATCCCGGAAATTATGTGCGTGTTTCGG
AACGAATCGCAAAAGAAACACCCAATTCCCTTTGGGCAAACCAATTTGATAACCTTGCCAACAGGAATGCTCACTTTGAA
ACCACAGGTCCCGAAATTTGGAACCAAACCCAAGGAAAAATTGATGTCTGGACCACTTCGATTGGAACGGGAGGGACATA
CGCCGGAACCGCTCTTTATCTCAAATCAAAAAATCCAAATGTAAAATGCATTGTGGCAGATCCGTATGGTTCTGGAGTGT
ATTCATTTGTGAAAACTGGAACGATTACGATCGAAGGATCTTCCATCACAGAAGGGATCGGGCAAGGTCGCATCACAAAA
AATATGGAAGGAATGCCTTCTGACGATGCCATTCGCATCCACGACAAACAAGCACTCGAAATCTTACAAAAGGTCCTCCG
AGAGGATGGACTCTTTATGGGAGGAAGTGTCGGAATCAATTTGGCAGCCGCCTTCCAAATCGCAAAAGAGATAGGACCTG
GACACACCATCGTGACTGTGTTATGTGACACAGGAACCAAATACCAATCCAAACTTTACAATCCTGATTTTTTGAAATCC
AAGGGACTTTTGTGA

Upstream 100 bases:

>100_bases
TTTTTGTGGGATCGCAGGAGCAGGTGCGAACCACTGCCGAATGGACTTGTACATAAACTTGGAAATTGAAGTCTAATAGA
GAGGAGTCAAGTGTATTCTT

Downstream 100 bases:

>100_bases
TGTGACGATGAGACAAAATTTGTCCAATTTTAGGCATCCAATGATTCCACTTGGCGACAAAAAAACTATCCATTCGAAAA
GAACCAAAAACAATCATCAG

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MQTEIRNGFIATIGNTPLIRIRSLSEETGCEILGKAEFLNPGGSVKDRAALYIIEDAEKRGLLKKGGTVVEGTAGNTGIG
LTHICNAKGYKSIIVIPETQSKEKIEMLRTLGAEVILVPAVPYVDPGNYVRVSERIAKETPNSLWANQFDNLANRNAHFE
TTGPEIWNQTQGKIDVWTTSIGTGGTYAGTALYLKSKNPNVKCIVADPYGSGVYSFVKTGTITIEGSSITEGIGQGRITK
NMEGMPSDDAIRIHDKQALEILQKVLREDGLFMGGSVGINLAAAFQIAKEIGPGHTIVTVLCDTGTKYQSKLYNPDFLKS
KGLL

Sequences:

>Translated_324_residues
MQTEIRNGFIATIGNTPLIRIRSLSEETGCEILGKAEFLNPGGSVKDRAALYIIEDAEKRGLLKKGGTVVEGTAGNTGIG
LTHICNAKGYKSIIVIPETQSKEKIEMLRTLGAEVILVPAVPYVDPGNYVRVSERIAKETPNSLWANQFDNLANRNAHFE
TTGPEIWNQTQGKIDVWTTSIGTGGTYAGTALYLKSKNPNVKCIVADPYGSGVYSFVKTGTITIEGSSITEGIGQGRITK
NMEGMPSDDAIRIHDKQALEILQKVLREDGLFMGGSVGINLAAAFQIAKEIGPGHTIVTVLCDTGTKYQSKLYNPDFLKS
KGLL
>Mature_324_residues
MQTEIRNGFIATIGNTPLIRIRSLSEETGCEILGKAEFLNPGGSVKDRAALYIIEDAEKRGLLKKGGTVVEGTAGNTGIG
LTHICNAKGYKSIIVIPETQSKEKIEMLRTLGAEVILVPAVPYVDPGNYVRVSERIAKETPNSLWANQFDNLANRNAHFE
TTGPEIWNQTQGKIDVWTTSIGTGGTYAGTALYLKSKNPNVKCIVADPYGSGVYSFVKTGTITIEGSSITEGIGQGRITK
NMEGMPSDDAIRIHDKQALEILQKVLREDGLFMGGSVGINLAAAFQIAKEIGPGHTIVTVLCDTGTKYQSKLYNPDFLKS
KGLL

Specific function: Two Cysteine Synthase Enzymes Are Found. Both Catalyze The Same Reaction. Cysteine Synthase B Can Also Use Thiosulfate In Place Of Sulfide To Give Cysteine Thiosulfonate As A Product. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=324, Percent_Identity=38.8888888888889, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI295821200, Length=324, Percent_Identity=38.8888888888889, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI4557415, Length=324, Percent_Identity=38.8888888888889, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI11345492, Length=200, Percent_Identity=29, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI2367138, Length=303, Percent_Identity=35.6435643564356, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1788754, Length=315, Percent_Identity=35.5555555555556, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17562970, Length=307, Percent_Identity=40.0651465798046, Blast_Score=201, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI32566674, Length=300, Percent_Identity=40.3333333333333, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17535051, Length=304, Percent_Identity=38.4868421052632, Blast_Score=197, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI115535073, Length=310, Percent_Identity=37.0967741935484, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17561720, Length=307, Percent_Identity=38.4364820846906, Blast_Score=172, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17534315, Length=332, Percent_Identity=32.2289156626506, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI25147552, Length=329, Percent_Identity=31.306990881459, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32566672, Length=222, Percent_Identity=35.5855855855856, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71996324, Length=341, Percent_Identity=24.9266862170088, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321449, Length=331, Percent_Identity=41.9939577039275, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6321594, Length=334, Percent_Identity=32.6347305389222, Blast_Score=176, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24643623, Length=325, Percent_Identity=35.6923076923077, Blast_Score=194, Evalue=5e-50,
Organism=Drosophila melanogaster, GI20129101, Length=325, Percent_Identity=35.6923076923077, Blast_Score=194, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34907; Mature: 34907

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTEIRNGFIATIGNTPLIRIRSLSEETGCEILGKAEFLNPGGSVKDRAALYIIEDAEKR
CCCCCCCCEEEEECCCCEEEEECCCCCCCCEEEECEEEECCCCCCCCCEEEEEEECCCCC
GLLKKGGTVVEGTAGNTGIGLTHICNAKGYKSIIVIPETQSKEKIEMLRTLGAEVILVPA
CHHHCCCEEEECCCCCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEECC
VPYVDPGNYVRVSERIAKETPNSLWANQFDNLANRNAHFETTGPEIWNQTQGKIDVWTTS
CCCCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHCCCCCEECCCHHHHCCCCCEEEEEEEE
IGTGGTYAGTALYLKSKNPNVKCIVADPYGSGVYSFVKTGTITIEGSSITEGIGQGRITK
CCCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHHCCCEEEEECCHHHHCCCCCCEEC
NMEGMPSDDAIRIHDKQALEILQKVLREDGLFMGGSVGINLAAAFQIAKEIGPGHTIVTV
CCCCCCCCCEEEEECHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCCEEEEE
LCDTGTKYQSKLYNPDFLKSKGLL
EECCCCHHHHHCCCCCHHHCCCCC
>Mature Secondary Structure
MQTEIRNGFIATIGNTPLIRIRSLSEETGCEILGKAEFLNPGGSVKDRAALYIIEDAEKR
CCCCCCCCEEEEECCCCEEEEECCCCCCCCEEEECEEEECCCCCCCCCEEEEEEECCCCC
GLLKKGGTVVEGTAGNTGIGLTHICNAKGYKSIIVIPETQSKEKIEMLRTLGAEVILVPA
CHHHCCCEEEECCCCCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEECC
VPYVDPGNYVRVSERIAKETPNSLWANQFDNLANRNAHFETTGPEIWNQTQGKIDVWTTS
CCCCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHCCCCCEECCCHHHHCCCCCEEEEEEEE
IGTGGTYAGTALYLKSKNPNVKCIVADPYGSGVYSFVKTGTITIEGSSITEGIGQGRITK
CCCCCCCCCEEEEEECCCCCEEEEEECCCCCCHHHHHCCCEEEEECCHHHHCCCCCCEEC
NMEGMPSDDAIRIHDKQALEILQKVLREDGLFMGGSVGINLAAAFQIAKEIGPGHTIVTV
CCCCCCCCCEEEEECHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCCEEEEE
LCDTGTKYQSKLYNPDFLKSKGLL
EECCCCHHHHHCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA