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The map label for this gene is leuA2 [H]

Identifier: 183220507

GI number: 183220507

Start: 1154259

End: 1155443

Strand: Reverse

Name: leuA2 [H]

Synonym: LEPBI_I1108

Alternate gene names: 183220507

Gene position: 1155443-1154259 (Counterclockwise)

Preceding gene: 183220508

Following gene: 183220506

Centisome position: 32.1

GC content: 41.18

Gene sequence:

>1185_bases
ATGAAACCAAAAACCATTCACATCCAAGACGTCACCTTACGGGACGGAAACCAAGCGTTAAAACGACCATGGACCATCGA
TGAAAAAATCGAAGTCTTTGATTTGCTCGTCGAACTTAACGTGGATGGGATCGAAGTTGGATTCCCTTCTTCAAATGAAA
CCGAATTCCATACCTGCCAGGTTTTGTCCAAACGTGCTCCCAAAGGAAAACCAATTGCAGCATTATCGAGAGCCAATCAA
AACGAAATCGCTGTGACTTGGGAAGCCATCCAAAAAGCAGATTGTCCTCGTATGCACATCGTTTACCCAGTCAGTGATTT
TTCCATTAAACATGTGTTAAAGATTTCTGAAAAAGAGGTACTTCAAAAAATTCGGAATTCCATTTCCTTTGCTCGCTCCA
TTGTGGGACCTGGAATCGAAATCCAATTTTCAGGAGAACATTTTGGAGATGCCATTGAAAACTTTGCATTCACGAAAGAA
GCATTTTTGACGGCCATTGAAGCTGGTGCCAACATCATCAATTTACCGAATACCGTGGAAAGGTATCGGCCGATGGTTTT
TGTCAATATGGTCAAAGAAATCAAAGATGTCGTAAAAGACAAAGCCATCATATCAATTCACACTCATAATGATTTGGGTA
TGGCAACGGCGACTTCAGTTGAATCCGTCTATGTGGGTGCAGAACAAATCGAAGTGGCCTTAAATGGTCTTGGTGAGAGA
GCTGGAAATACCAATTTATATGAAACGGCAATCGCCTTACACCAGAATGGCGAAAATTTGAATATCAACTTCCAAAGAAT
TTATCCTACTGCAAAACGAATCTCCGAATTAACAGGAATTCCGATCGGAGAAAAAACACCGATCATCGGAGAGGATATCT
TTTCACATAGATCTGGGATCCATCAAGATGGTGTGACAAAAACCCTACACCAATCAAAAGGTGCGTATCGGACCTTTTCA
CCTGAATTTGTTGGCCGAATGGACAAAGAAACGATTTCGTTCACCAATCAATCAGGACACAAAGCGATAGAGTTTTTATT
ACACCAAAGAGGGATCCAAGTTTCAAAAGAAGGAATTCACCATTTGTTCTCTTTGGCCAAATCGATCTCATCTCGTGAAA
ACAATCGAGAAATCACCGAAGCAGAGTTAGTGGCACTTAGCCAATCTTTACTCACCTATCAGTGA

Upstream 100 bases:

>100_bases
GAGTGCTATGTGACAAAGCCCACTCCCAACCGGGAAGTGGGCTTTTTTTATTTTGGTCCCCCGGTCGGAAGTGACAACTT
TTTGCGGATACGGAGGAACC

Downstream 100 bases:

>100_bases
ACAAAGAAGGCTTTTGTCCAGAGAAACTTAAGGCAAATGATCGGGAATCGTTGACAAATCTTTGTCAATCTGCCATAATT
TCGTTTGGTTTTTCTGGAGG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKPKTIHIQDVTLRDGNQALKRPWTIDEKIEVFDLLVELNVDGIEVGFPSSNETEFHTCQVLSKRAPKGKPIAALSRANQ
NEIAVTWEAIQKADCPRMHIVYPVSDFSIKHVLKISEKEVLQKIRNSISFARSIVGPGIEIQFSGEHFGDAIENFAFTKE
AFLTAIEAGANIINLPNTVERYRPMVFVNMVKEIKDVVKDKAIISIHTHNDLGMATATSVESVYVGAEQIEVALNGLGER
AGNTNLYETAIALHQNGENLNINFQRIYPTAKRISELTGIPIGEKTPIIGEDIFSHRSGIHQDGVTKTLHQSKGAYRTFS
PEFVGRMDKETISFTNQSGHKAIEFLLHQRGIQVSKEGIHHLFSLAKSISSRENNREITEAELVALSQSLLTYQ

Sequences:

>Translated_394_residues
MKPKTIHIQDVTLRDGNQALKRPWTIDEKIEVFDLLVELNVDGIEVGFPSSNETEFHTCQVLSKRAPKGKPIAALSRANQ
NEIAVTWEAIQKADCPRMHIVYPVSDFSIKHVLKISEKEVLQKIRNSISFARSIVGPGIEIQFSGEHFGDAIENFAFTKE
AFLTAIEAGANIINLPNTVERYRPMVFVNMVKEIKDVVKDKAIISIHTHNDLGMATATSVESVYVGAEQIEVALNGLGER
AGNTNLYETAIALHQNGENLNINFQRIYPTAKRISELTGIPIGEKTPIIGEDIFSHRSGIHQDGVTKTLHQSKGAYRTFS
PEFVGRMDKETISFTNQSGHKAIEFLLHQRGIQVSKEGIHHLFSLAKSISSRENNREITEAELVALSQSLLTYQ
>Mature_394_residues
MKPKTIHIQDVTLRDGNQALKRPWTIDEKIEVFDLLVELNVDGIEVGFPSSNETEFHTCQVLSKRAPKGKPIAALSRANQ
NEIAVTWEAIQKADCPRMHIVYPVSDFSIKHVLKISEKEVLQKIRNSISFARSIVGPGIEIQFSGEHFGDAIENFAFTKE
AFLTAIEAGANIINLPNTVERYRPMVFVNMVKEIKDVVKDKAIISIHTHNDLGMATATSVESVYVGAEQIEVALNGLGER
AGNTNLYETAIALHQNGENLNINFQRIYPTAKRISELTGIPIGEKTPIIGEDIFSHRSGIHQDGVTKTLHQSKGAYRTFS
PEFVGRMDKETISFTNQSGHKAIEFLLHQRGIQVSKEGIHHLFSLAKSISSRENNREITEAELVALSQSLLTYQ

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=363, Percent_Identity=34.9862258953168, Blast_Score=204, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6324225, Length=403, Percent_Identity=27.2952853598015, Blast_Score=142, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6324682, Length=407, Percent_Identity=27.5184275184275, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320071, Length=373, Percent_Identity=24.9329758713137, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320019, Length=367, Percent_Identity=25.0681198910082, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 43923; Mature: 43923

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPKTIHIQDVTLRDGNQALKRPWTIDEKIEVFDLLVELNVDGIEVGFPSSNETEFHTCQ
CCCCEEEEEEEEECCCCHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHH
VLSKRAPKGKPIAALSRANQNEIAVTWEAIQKADCPRMHIVYPVSDFSIKHVLKISEKEV
HHHHCCCCCCCCHHHHCCCCCCEEEEHHHHHCCCCCCEEEEEECCCCCHHHHHHCCHHHH
LQKIRNSISFARSIVGPGIEIQFSGEHFGDAIENFAFTKEAFLTAIEAGANIINLPNTVE
HHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHH
RYRPMVFVNMVKEIKDVVKDKAIISIHTHNDLGMATATSVESVYVGAEQIEVALNGLGER
HHCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHEECHHHHHHHHHHHHCC
AGNTNLYETAIALHQNGENLNINFQRIYPTAKRISELTGIPIGEKTPIIGEDIFSHRSGI
CCCCCHHHHHHHEECCCCEEEEEEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
HQDGVTKTLHQSKGAYRTFSPEFVGRMDKETISFTNQSGHKAIEFLLHQRGIQVSKEGIH
CCCCHHHHHHHCCCCCCCCCHHHHCCCCCHHHEECCCCHHHHHHHHHHHCCCCHHHHHHH
HLFSLAKSISSRENNREITEAELVALSQSLLTYQ
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPKTIHIQDVTLRDGNQALKRPWTIDEKIEVFDLLVELNVDGIEVGFPSSNETEFHTCQ
CCCCEEEEEEEEECCCCHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHH
VLSKRAPKGKPIAALSRANQNEIAVTWEAIQKADCPRMHIVYPVSDFSIKHVLKISEKEV
HHHHCCCCCCCCHHHHCCCCCCEEEEHHHHHCCCCCCEEEEEECCCCCHHHHHHCCHHHH
LQKIRNSISFARSIVGPGIEIQFSGEHFGDAIENFAFTKEAFLTAIEAGANIINLPNTVE
HHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHH
RYRPMVFVNMVKEIKDVVKDKAIISIHTHNDLGMATATSVESVYVGAEQIEVALNGLGER
HHCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHEECHHHHHHHHHHHHCC
AGNTNLYETAIALHQNGENLNINFQRIYPTAKRISELTGIPIGEKTPIIGEDIFSHRSGI
CCCCCHHHHHHHEECCCCEEEEEEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
HQDGVTKTLHQSKGAYRTFSPEFVGRMDKETISFTNQSGHKAIEFLLHQRGIQVSKEGIH
CCCCHHHHHHHCCCCCCCCCHHHHCCCCCHHHEECCCCHHHHHHHHHHHCCCCHHHHHHH
HLFSLAKSISSRENNREITEAELVALSQSLLTYQ
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA