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The map label for this gene is gyrB2 [H]

Identifier: 183220092

GI number: 183220092

Start: 686176

End: 688302

Strand: Direct

Name: gyrB2 [H]

Synonym: LEPBI_I0680

Alternate gene names: 183220092

Gene position: 686176-688302 (Clockwise)

Preceding gene: 183220091

Following gene: 183220093

Centisome position: 19.06

GC content: 40.67

Gene sequence:

>2127_bases
ATGGCTCAAAAAACTGAAAAAACCTCAGGAAATTCGCGAAATTTTAAAAAATTATCTCACGTAGAACACGTTCGCATGCG
GACAGGGATGTGGCTTGGCCAAAACTCTTTGTCTACCTTTGAACAACATTTTTTCAAAAGAGACAATGCCGGAACGTACG
AAGTGTCCCATGAGGAACTATCAGACATTCCAGCTAAAATCAAATGTTTAGATGAAGCTTGTATGAACTGCGTGGATGAA
TATCGCAAAAACCTAAACGATAAAGCCATTCCCGAAAAAGACAAAATGAACAAACTCATCGTTCAATTGTCTACAGATCG
CAAACGAGTGACCATCCAAGACAATGGTCGTGGAATTCCTGCCGACAATGCGGAAGGGGTTTACCTCCACTTGATGTATG
GAGAAAACTTTGATGATTTAGCGAAAGAAGACCACGTAGCAGGACAAAATGGTGTGGGGATCTCACTCGTTCGTATGGTC
TCTTCTTTCTTTCGAGTCAAAACCATCAATGGTGGCAAAGCATACAAAAAGATGTTTAGCATCCATGATGATGTAAAAAA
AGTCATTCGCAGTTTCAAACTTTCAAAAGAAGATACAGAACGTGTTCACTTATATTATGATGAACATGGAACCTTTGTGG
ATTGTCCTTTGCTTTCCGCAGACCAAATCAAACAACTAAAAGCACCTTGCGACAAAACAGGGATGACCGCCGTTGTAGAA
ACAGCCAAAAAAGAAGACCACGGGACTACGGTGGAATTTGAACTCAATTCTGCATACTTTAACAACCTGGATACTTCCTT
TAACATCAACTTGGTGAAACAATACTTACAAGACATCGCAATGTCTAATCCTGGTTTGGAAGTGGTTTTTATCCACAAAA
CAGGGAAAGAAAAGTATAAATTCAAAAAAGGTTTTGATGAGATTTTTAGTAACTCTGAGATGGTTTACTATAAATTAGAT
TATTCTGATAAGGCTTCCGCTTCACAAATCCATATGGATACGTATGTGGTTGTGGGCCAAAACAAAAACCTAACTTGGGT
AAACTCAATTTTTTGCCCTCAAGGTGGATCTGCCATTGAGTATTTGGAAAACAGGCTTTGTGATGAGATCCGTAAAAAAT
CTCAAATTGTCAGTTTAGAAAAAAAACTGAATACACAATGCACTCGTAATGATGTCAGAAGTTGTTTTCACATGTATGTG
AACCTTCGGATCCTAAACCCTCGCTTTAAATCCCAGGACAAGTCTTATCTCATCAATGATTTGAATGAAGACATCCGTAA
GTCCGTTGACAAACATTTGGACAAACTTTTGAAAAAAACTGGCCTCATCGAAGAAATTAAGATGGTGATGGAGCGCAGAA
CCCAGATGAAACAGCTCGAGGATGCGCAGAAAGGCCTCCGTAAGGCGTCCCGGAACAATATCCCTAAGCTGATGCCTCCG
ACAGGCAAACCAAACGATCCAGGCCGAATTCTCTTCGTAGCAGAAGGGGACTCGGCGATTGCGGGACTACGTCCAGCAAG
GAATCCTAAATTACACGGACTTTTCCCACTTCGAGGGAAACCACTCAATTGTAAAGGGATGTCTCTTGCCAAGGCATTAC
AAAACGAAGAGATGAAAAACATCGTCGCCATTGTGGGCCTTCCTCTGGACCAAAAAGTAAAGTCCATTGATGAACTGAAC
TATGACAAAATTAGTATCATCACCGATGCGGATTTTGACGGGTATGCAATTCGTTCCCTAATGTTATCTTTCTTTTACGA
GTATTGGCCAGAGCTCTTTGAGTTAGGTTTTATAAATATCTCTGCGGCACCATTGTATGAAGTGGATGTGAAGTGGAAAG
ATGCCAAAAAAGAAACTGTTTTCTGTATTGATGACTCCGACTACGACAAGTTAGTTGCACGAGTCAACAAACAAGGTGCG
GAAATCACTCGTAAAAAACGGAACAAAGGACTTGGAGAAACAGGGAAAGAAGCCATGAAGTATGCTGTGGATCATTGTAT
GACCACGATCACCATTGGTAACAAAAAAACTGCCAAAACCACCCAAGACTTATGGTTCCATAAAGATTATGCAGAAAAAC
GTAGGGAAGCCATTTCCGAATACTCGATGAGTGTGATCCAAGACTAA

Upstream 100 bases:

>100_bases
CGAATACACAACCTTACAAAAAGATGATAAAAAACTCACTGGTTTTATGACCGATGAGTTAGATGAACTGAAGAAAAAAT
GGGATCCGAAATAAATATCT

Downstream 100 bases:

>100_bases
AACAGATTGCCACTCCTCTAGAATCGTTCCAACAAACAGAGTCCAGACGAAAGGACTCGATTGTTGTTCTTTCTCTTTTG
CATAGCGATCACTCCCCAAT

Product: DNA gyrase/topoisomerase subunit B

Products: NA

Alternate protein names: Topoisomerase II subunit B [H]

Number of amino acids: Translated: 708; Mature: 707

Protein sequence:

>708_residues
MAQKTEKTSGNSRNFKKLSHVEHVRMRTGMWLGQNSLSTFEQHFFKRDNAGTYEVSHEELSDIPAKIKCLDEACMNCVDE
YRKNLNDKAIPEKDKMNKLIVQLSTDRKRVTIQDNGRGIPADNAEGVYLHLMYGENFDDLAKEDHVAGQNGVGISLVRMV
SSFFRVKTINGGKAYKKMFSIHDDVKKVIRSFKLSKEDTERVHLYYDEHGTFVDCPLLSADQIKQLKAPCDKTGMTAVVE
TAKKEDHGTTVEFELNSAYFNNLDTSFNINLVKQYLQDIAMSNPGLEVVFIHKTGKEKYKFKKGFDEIFSNSEMVYYKLD
YSDKASASQIHMDTYVVVGQNKNLTWVNSIFCPQGGSAIEYLENRLCDEIRKKSQIVSLEKKLNTQCTRNDVRSCFHMYV
NLRILNPRFKSQDKSYLINDLNEDIRKSVDKHLDKLLKKTGLIEEIKMVMERRTQMKQLEDAQKGLRKASRNNIPKLMPP
TGKPNDPGRILFVAEGDSAIAGLRPARNPKLHGLFPLRGKPLNCKGMSLAKALQNEEMKNIVAIVGLPLDQKVKSIDELN
YDKISIITDADFDGYAIRSLMLSFFYEYWPELFELGFINISAAPLYEVDVKWKDAKKETVFCIDDSDYDKLVARVNKQGA
EITRKKRNKGLGETGKEAMKYAVDHCMTTITIGNKKTAKTTQDLWFHKDYAEKRREAISEYSMSVIQD

Sequences:

>Translated_708_residues
MAQKTEKTSGNSRNFKKLSHVEHVRMRTGMWLGQNSLSTFEQHFFKRDNAGTYEVSHEELSDIPAKIKCLDEACMNCVDE
YRKNLNDKAIPEKDKMNKLIVQLSTDRKRVTIQDNGRGIPADNAEGVYLHLMYGENFDDLAKEDHVAGQNGVGISLVRMV
SSFFRVKTINGGKAYKKMFSIHDDVKKVIRSFKLSKEDTERVHLYYDEHGTFVDCPLLSADQIKQLKAPCDKTGMTAVVE
TAKKEDHGTTVEFELNSAYFNNLDTSFNINLVKQYLQDIAMSNPGLEVVFIHKTGKEKYKFKKGFDEIFSNSEMVYYKLD
YSDKASASQIHMDTYVVVGQNKNLTWVNSIFCPQGGSAIEYLENRLCDEIRKKSQIVSLEKKLNTQCTRNDVRSCFHMYV
NLRILNPRFKSQDKSYLINDLNEDIRKSVDKHLDKLLKKTGLIEEIKMVMERRTQMKQLEDAQKGLRKASRNNIPKLMPP
TGKPNDPGRILFVAEGDSAIAGLRPARNPKLHGLFPLRGKPLNCKGMSLAKALQNEEMKNIVAIVGLPLDQKVKSIDELN
YDKISIITDADFDGYAIRSLMLSFFYEYWPELFELGFINISAAPLYEVDVKWKDAKKETVFCIDDSDYDKLVARVNKQGA
EITRKKRNKGLGETGKEAMKYAVDHCMTTITIGNKKTAKTTQDLWFHKDYAEKRREAISEYSMSVIQD
>Mature_707_residues
AQKTEKTSGNSRNFKKLSHVEHVRMRTGMWLGQNSLSTFEQHFFKRDNAGTYEVSHEELSDIPAKIKCLDEACMNCVDEY
RKNLNDKAIPEKDKMNKLIVQLSTDRKRVTIQDNGRGIPADNAEGVYLHLMYGENFDDLAKEDHVAGQNGVGISLVRMVS
SFFRVKTINGGKAYKKMFSIHDDVKKVIRSFKLSKEDTERVHLYYDEHGTFVDCPLLSADQIKQLKAPCDKTGMTAVVET
AKKEDHGTTVEFELNSAYFNNLDTSFNINLVKQYLQDIAMSNPGLEVVFIHKTGKEKYKFKKGFDEIFSNSEMVYYKLDY
SDKASASQIHMDTYVVVGQNKNLTWVNSIFCPQGGSAIEYLENRLCDEIRKKSQIVSLEKKLNTQCTRNDVRSCFHMYVN
LRILNPRFKSQDKSYLINDLNEDIRKSVDKHLDKLLKKTGLIEEIKMVMERRTQMKQLEDAQKGLRKASRNNIPKLMPPT
GKPNDPGRILFVAEGDSAIAGLRPARNPKLHGLFPLRGKPLNCKGMSLAKALQNEEMKNIVAIVGLPLDQKVKSIDELNY
DKISIITDADFDGYAIRSLMLSFFYEYWPELFELGFINISAAPLYEVDVKWKDAKKETVFCIDDSDYDKLVARVNKQGAE
ITRKKRNKGLGETGKEAMKYAVDHCMTTITIGNKKTAKTTQDLWFHKDYAEKRREAISEYSMSVIQD

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=723, Percent_Identity=24.3430152143845, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI19913406, Length=336, Percent_Identity=28.8690476190476, Blast_Score=103, Evalue=8e-22,
Organism=Escherichia coli, GI48994957, Length=672, Percent_Identity=24.4047619047619, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI1789408, Length=636, Percent_Identity=21.5408805031447, Blast_Score=111, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI212645845, Length=625, Percent_Identity=26.08, Blast_Score=116, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17535065, Length=376, Percent_Identity=27.3936170212766, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI212645657, Length=265, Percent_Identity=29.811320754717, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=387, Percent_Identity=25.5813953488372, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI17136538, Length=622, Percent_Identity=24.2765273311897, Blast_Score=122, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 81004; Mature: 80873

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKTEKTSGNSRNFKKLSHVEHVRMRTGMWLGQNSLSTFEQHFFKRDNAGTYEVSHEEL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEECCCHHHHHHHHHHHCCCCCCEEECHHHH
SDIPAKIKCLDEACMNCVDEYRKNLNDKAIPEKDKMNKLIVQLSTDRKRVTIQDNGRGIP
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCEEEEEEECCCEEEEECCCCCCCC
ADNAEGVYLHLMYGENFDDLAKEDHVAGQNGVGISLVRMVSSFFRVKTINGGKAYKKMFS
CCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEECCCCHHHHHHHH
IHDDVKKVIRSFKLSKEDTERVHLYYDEHGTFVDCPLLSADQIKQLKAPCDKTGMTAVVE
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCCCCHHHHHHHHCCCCCCCCHHHHH
TAKKEDHGTTVEFELNSAYFNNLDTSFNINLVKQYLQDIAMSNPGLEVVFIHKTGKEKYK
HHHCCCCCCEEEEEECCHHHCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHH
FKKGFDEIFSNSEMVYYKLDYSDKASASQIHMDTYVVVGQNKNLTWVNSIFCPQGGSAIE
HHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEEEEECCCCEEEEEEEECCCCCHHHH
YLENRLCDEIRKKSQIVSLEKKLNTQCTRNDVRSCFHMYVNLRILNPRFKSQDKSYLIND
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHH
LNEDIRKSVDKHLDKLLKKTGLIEEIKMVMERRTQMKQLEDAQKGLRKASRNNIPKLMPP
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TGKPNDPGRILFVAEGDSAIAGLRPARNPKLHGLFPLRGKPLNCKGMSLAKALQNEEMKN
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHH
IVAIVGLPLDQKVKSIDELNYDKISIITDADFDGYAIRSLMLSFFYEYWPELFELGFINI
EEHEECCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
SAAPLYEVDVKWKDAKKETVFCIDDSDYDKLVARVNKQGAEITRKKRNKGLGETGKEAMK
CCCCEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHH
YAVDHCMTTITIGNKKTAKTTQDLWFHKDYAEKRREAISEYSMSVIQD
HHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQKTEKTSGNSRNFKKLSHVEHVRMRTGMWLGQNSLSTFEQHFFKRDNAGTYEVSHEEL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCEECCCHHHHHHHHHHHCCCCCCEEECHHHH
SDIPAKIKCLDEACMNCVDEYRKNLNDKAIPEKDKMNKLIVQLSTDRKRVTIQDNGRGIP
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCEEEEEEECCCEEEEECCCCCCCC
ADNAEGVYLHLMYGENFDDLAKEDHVAGQNGVGISLVRMVSSFFRVKTINGGKAYKKMFS
CCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHEEEECCCCHHHHHHHH
IHDDVKKVIRSFKLSKEDTERVHLYYDEHGTFVDCPLLSADQIKQLKAPCDKTGMTAVVE
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCCCCHHHHHHHHCCCCCCCCHHHHH
TAKKEDHGTTVEFELNSAYFNNLDTSFNINLVKQYLQDIAMSNPGLEVVFIHKTGKEKYK
HHHCCCCCCEEEEEECCHHHCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHH
FKKGFDEIFSNSEMVYYKLDYSDKASASQIHMDTYVVVGQNKNLTWVNSIFCPQGGSAIE
HHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEEEEECCCCEEEEEEEECCCCCHHHH
YLENRLCDEIRKKSQIVSLEKKLNTQCTRNDVRSCFHMYVNLRILNPRFKSQDKSYLIND
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHH
LNEDIRKSVDKHLDKLLKKTGLIEEIKMVMERRTQMKQLEDAQKGLRKASRNNIPKLMPP
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TGKPNDPGRILFVAEGDSAIAGLRPARNPKLHGLFPLRGKPLNCKGMSLAKALQNEEMKN
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHH
IVAIVGLPLDQKVKSIDELNYDKISIITDADFDGYAIRSLMLSFFYEYWPELFELGFINI
EEHEECCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
SAAPLYEVDVKWKDAKKETVFCIDDSDYDKLVARVNKQGAEITRKKRNKGLGETGKEAMK
CCCCEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHH
YAVDHCMTTITIGNKKTAKTTQDLWFHKDYAEKRREAISEYSMSVIQD
HHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8863738; 10360571 [H]