Click here to switch to the map view.

The map label for this gene is gyrA2 [H]

Identifier: 183220091

GI number: 183220091

Start: 684784

End: 686169

Strand: Direct

Name: gyrA2 [H]

Synonym: LEPBI_I0679

Alternate gene names: 183220091

Gene position: 684784-686169 (Clockwise)

Preceding gene: 183220079

Following gene: 183220092

Centisome position: 19.02

GC content: 39.11

Gene sequence:

>1386_bases
GTGTCATTTGGAACCATGAAGAACGAAGAACAGTATCCGAAACGCCCCTTTGAAGACCAAGTAAACGACGACCAAAGGAA
ATACTCCCGATATGTGTGCGATTCGAGAGCCATACCGCAAGAAATTGATGGCCTAAAGCCTGTTCAGCGACGAATTCTAT
GGGCTATGTGGAATTCAGATGCAAGAAACCGTCATACAAAAACAGTAAAAGTCGCGGGGCTTGCGATGGGATACCATCCA
CATGGAGACAGATCCATCCAAGATGCTCTTTCCCAAATGGCACAAGACTTTGCCTTTGCGAATAATTATCCTTTGGTACA
TGGAGAAGGAACTTTTGGTGATGTCCTTGATCCCAATGCAATTGCATCCCCTCGTTATACAGAAGTCAAACTTTCTGACT
TTGCAAAAGACTTAGGTTTTTTTGAAAGTTTGCCTGACATTGATTATGTAAAAAACTACGATGAAACAGAAGACGAACCC
ATTCATTTTGTAGGAAAGGTTCCTGTCGTTTTACTCAATAACATCCAAGGGATTGCAACGGGTTTCCGTTGTTTTATTCC
AGCTCATAAACTGAGTGATGTGATCGAATCCCAAGTCACTTATTTAAAAACTGGTAAACCAAAAAAGATCACTCCATGGT
ACAAAGGGTACGGTGGAGAAGTGAAATTGTCAAAAAATGACAATGGAAACACTGTGATGTCCACCACATTCGGATTCAAA
AAAGAAGACGGGAAATTGTATCTTGTTGATTCTCCGATGAATTGGAACCGAGAAAAAGTGGTGAACTACTTAGATGATTT
GATTGAAAGAAAAGACAATTGGTTGAAAGACTACGTGGACCATTCCAGCCAAACCTTCAAAATCGAACTCATTGCTAAAA
AAGGCGAAGAACCATCCGAAAAAGAAATTAAAGAACTTTTTACCAAAGAAAACAATGAAGTGCTTACAATCAACGTCATC
ACTCACGAAGGAAAACTTCGTAACTTTGGTCCAGAAGAGATCATCAAACGGTTTTGTGATTTCCGTAAAACCCATCTCAT
CCGTCGTTTCAAACGATTGGCGGGACTTGAAAAAGAAAAAATTGATCGAAACTCAGAACTCATTCGCTTCATCAAAGAAA
GATGGAACGAAAAAGTAACAGGGATTAAATCCAAAAAAGAATTTGAAGATAAGTTAAAAGCAGCCAAGTTCGTCTACTTC
GAATGGTTGAGTTCCATTCCCGTTTATAGAATGACTTTGGAAGAAGTTCGTAAATGCGAAGATGCGATCGTGGAAGCAAA
AACCAAATACACCGAATACACAACCTTACAAAAAGATGATAAAAAACTCACTGGTTTTATGACCGATGAGTTAGATGAAC
TGAAGAAAAAATGGGATCCGAAATAA

Upstream 100 bases:

>100_bases
TGAAAACGGGAGTATTCTTGAGTTTGTGGGGACATAGATAGGTGCGTATCGCCAGTCTAAAAAGGGAAAACCGCTTGACA
AGTCAATTCATCGCACCCCT

Downstream 100 bases:

>100_bases
ATATCTATGGCTCAAAAAACTGAAAAAACCTCAGGAAATTCGCGAAATTTTAAAAAATTATCTCACGTAGAACACGTTCG
CATGCGGACAGGGATGTGGC

Product: DNA gyrase/topoisomerase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSFGTMKNEEQYPKRPFEDQVNDDQRKYSRYVCDSRAIPQEIDGLKPVQRRILWAMWNSDARNRHTKTVKVAGLAMGYHP
HGDRSIQDALSQMAQDFAFANNYPLVHGEGTFGDVLDPNAIASPRYTEVKLSDFAKDLGFFESLPDIDYVKNYDETEDEP
IHFVGKVPVVLLNNIQGIATGFRCFIPAHKLSDVIESQVTYLKTGKPKKITPWYKGYGGEVKLSKNDNGNTVMSTTFGFK
KEDGKLYLVDSPMNWNREKVVNYLDDLIERKDNWLKDYVDHSSQTFKIELIAKKGEEPSEKEIKELFTKENNEVLTINVI
THEGKLRNFGPEEIIKRFCDFRKTHLIRRFKRLAGLEKEKIDRNSELIRFIKERWNEKVTGIKSKKEFEDKLKAAKFVYF
EWLSSIPVYRMTLEEVRKCEDAIVEAKTKYTEYTTLQKDDKKLTGFMTDELDELKKKWDPK

Sequences:

>Translated_461_residues
MSFGTMKNEEQYPKRPFEDQVNDDQRKYSRYVCDSRAIPQEIDGLKPVQRRILWAMWNSDARNRHTKTVKVAGLAMGYHP
HGDRSIQDALSQMAQDFAFANNYPLVHGEGTFGDVLDPNAIASPRYTEVKLSDFAKDLGFFESLPDIDYVKNYDETEDEP
IHFVGKVPVVLLNNIQGIATGFRCFIPAHKLSDVIESQVTYLKTGKPKKITPWYKGYGGEVKLSKNDNGNTVMSTTFGFK
KEDGKLYLVDSPMNWNREKVVNYLDDLIERKDNWLKDYVDHSSQTFKIELIAKKGEEPSEKEIKELFTKENNEVLTINVI
THEGKLRNFGPEEIIKRFCDFRKTHLIRRFKRLAGLEKEKIDRNSELIRFIKERWNEKVTGIKSKKEFEDKLKAAKFVYF
EWLSSIPVYRMTLEEVRKCEDAIVEAKTKYTEYTTLQKDDKKLTGFMTDELDELKKKWDPK
>Mature_460_residues
SFGTMKNEEQYPKRPFEDQVNDDQRKYSRYVCDSRAIPQEIDGLKPVQRRILWAMWNSDARNRHTKTVKVAGLAMGYHPH
GDRSIQDALSQMAQDFAFANNYPLVHGEGTFGDVLDPNAIASPRYTEVKLSDFAKDLGFFESLPDIDYVKNYDETEDEPI
HFVGKVPVVLLNNIQGIATGFRCFIPAHKLSDVIESQVTYLKTGKPKKITPWYKGYGGEVKLSKNDNGNTVMSTTFGFKK
EDGKLYLVDSPMNWNREKVVNYLDDLIERKDNWLKDYVDHSSQTFKIELIAKKGEEPSEKEIKELFTKENNEVLTINVIT
HEGKLRNFGPEEIIKRFCDFRKTHLIRRFKRLAGLEKEKIDRNSELIRFIKERWNEKVTGIKSKKEFEDKLKAAKFVYFE
WLSSIPVYRMTLEEVRKCEDAIVEAKTKYTEYTTLQKDDKKLTGFMTDELDELKKKWDPK

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=371, Percent_Identity=25.0673854447439, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI19913406, Length=367, Percent_Identity=25.8855585831063, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1789396, Length=457, Percent_Identity=27.7899343544858, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1788562, Length=361, Percent_Identity=28.5318559556787, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17535065, Length=395, Percent_Identity=28.1012658227848, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI212645657, Length=367, Percent_Identity=27.5204359673025, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI212645845, Length=440, Percent_Identity=25.4545454545455, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI115534553, Length=213, Percent_Identity=30.9859154929577, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=215, Percent_Identity=26.9767441860465, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17136538, Length=377, Percent_Identity=25.1989389920424, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 53788; Mature: 53657

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFGTMKNEEQYPKRPFEDQVNDDQRKYSRYVCDSRAIPQEIDGLKPVQRRILWAMWNSD
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHCCC
ARNRHTKTVKVAGLAMGYHPHGDRSIQDALSQMAQDFAFANNYPLVHGEGTFGDVLDPNA
CCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCC
IASPRYTEVKLSDFAKDLGFFESLPDIDYVKNYDETEDEPIHFVGKVPVVLLNNIQGIAT
CCCCCCCEEEHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEECCCHHHHHCCCHHHHC
GFRCFIPAHKLSDVIESQVTYLKTGKPKKITPWYKGYGGEVKLSKNDNGNTVMSTTFGFK
CHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCEEEEECCCCCCEEEEEECCCE
KEDGKLYLVDSPMNWNREKVVNYLDDLIERKDNWLKDYVDHSSQTFKIELIAKKGEEPSE
ECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCH
KEIKELFTKENNEVLTINVITHEGKLRNFGPEEIIKRFCDFRKTHLIRRFKRLAGLEKEK
HHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IDRNSELIRFIKERWNEKVTGIKSKKEFEDKLKAAKFVYFEWLSSIPVYRMTLEEVRKCE
HCCHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DAIVEAKTKYTEYTTLQKDDKKLTGFMTDELDELKKKWDPK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SFGTMKNEEQYPKRPFEDQVNDDQRKYSRYVCDSRAIPQEIDGLKPVQRRILWAMWNSD
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHCCC
ARNRHTKTVKVAGLAMGYHPHGDRSIQDALSQMAQDFAFANNYPLVHGEGTFGDVLDPNA
CCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCC
IASPRYTEVKLSDFAKDLGFFESLPDIDYVKNYDETEDEPIHFVGKVPVVLLNNIQGIAT
CCCCCCCEEEHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEECCCHHHHHCCCHHHHC
GFRCFIPAHKLSDVIESQVTYLKTGKPKKITPWYKGYGGEVKLSKNDNGNTVMSTTFGFK
CHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCEEEEECCCCCCEEEEEECCCE
KEDGKLYLVDSPMNWNREKVVNYLDDLIERKDNWLKDYVDHSSQTFKIELIAKKGEEPSE
ECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCH
KEIKELFTKENNEVLTINVITHEGKLRNFGPEEIIKRFCDFRKTHLIRRFKRLAGLEKEK
HHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IDRNSELIRFIKERWNEKVTGIKSKKEFEDKLKAAKFVYFEWLSSIPVYRMTLEEVRKCE
HCCHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DAIVEAKTKYTEYTTLQKDDKKLTGFMTDELDELKKKWDPK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8384814; 10688204 [H]