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The map label for this gene is miaA

Identifier: 183219896

GI number: 183219896

Start: 473441

End: 474331

Strand: Direct

Name: miaA

Synonym: LEPBI_I0478

Alternate gene names: 183219896

Gene position: 473441-474331 (Clockwise)

Preceding gene: 183219895

Following gene: 183219897

Centisome position: 13.15

GC content: 43.77

Gene sequence:

>891_bases
GTGATCCTTCCCATCCTTGGTGGACCCACCGGTTCTGGAAAAACTTCCCTCACACAAGTCCTAGACCCAAAACGTTTCGA
AATTGTTTCTTTTGACTCACGCCAAGTCTACCGGGACCTTCCGGTAGGCACAACGGCACCCACTCCCGAAGAGTGTTCTA
CGATTCGACATTGGCTCATTGGTTTTTTAAATGCCAACGAGTCCATCAATGCCAACCAGTTTTCCCTTTTAGCCCGCAAT
GCCATTGCTGACATCCAAAGTCGTGGAAAAATTCCTTTTCTCTTAGGAGGGACTGGTTTTTACCTACGAGCTTTTTTACT
CGGAATGTATCCCGTACCAACTGTACCCAAAGAAACAAAAGACTATGTATTCACACTTCCTTTGGAAGAGGCAAGGTCTC
AACTCCTGGCCAAAGATCCAAAGGCCATGGAATCTCTTTCCGACCAAGATGGATACCGCATCAAACGTGCTTTGGAAGTT
GTACTCACAGGAGTTTTGTGGTCAGATGTTTCCAAAGAAACTGTGGGAGGGTTTTGGAAAGACAATCCAGAAGTGAAAAT
CGTTGGTCATTGGTTGGATTGGCCCAGGGAAATTCTGTACCAACGCATCAACACTCGAGTGGAAACGATCATCAGAGGTA
TGTTAGAGGAAACGAAAGAAGTTTTATCCAAGTATGGTCCTGACTGTCCAGGGTTACGAACCTTAGGTTACAATTTTGCG
CTTGCATTCCTAAATGGAATGATAGACAGTAATACATTCATTGAACAGCTGGCGCAAAGTCACAGAAATTATGCAAAAAG
GCAAATCACTTGGTTCAAAAAAGAATCATTCCTTTCGCCCATTTCTTATGATGCCGCTGTCCAATTGTATACAAATATAG
AACAAAGATAG

Upstream 100 bases:

>100_bases
AAGAAGAGGAAGAACCAGTCATTGAAGATGATGAACTCTTAGAAGAAACGGAAGAATTGGAAGAGGCCGAAGAAGAGGAG
GTCGTTGCCGAGGAAGAGGA

Downstream 100 bases:

>100_bases
AGAGAACACTCGGGATTTCCAATGTCGGCAAAAAATAACATCCAAGACCAACTTTTAAATACAGCAAGAAAAGAGAAAAT
TGATCTCACCATCTACCTGT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MILPILGGPTGSGKTSLTQVLDPKRFEIVSFDSRQVYRDLPVGTTAPTPEECSTIRHWLIGFLNANESINANQFSLLARN
AIADIQSRGKIPFLLGGTGFYLRAFLLGMYPVPTVPKETKDYVFTLPLEEARSQLLAKDPKAMESLSDQDGYRIKRALEV
VLTGVLWSDVSKETVGGFWKDNPEVKIVGHWLDWPREILYQRINTRVETIIRGMLEETKEVLSKYGPDCPGLRTLGYNFA
LAFLNGMIDSNTFIEQLAQSHRNYAKRQITWFKKESFLSPISYDAAVQLYTNIEQR

Sequences:

>Translated_296_residues
MILPILGGPTGSGKTSLTQVLDPKRFEIVSFDSRQVYRDLPVGTTAPTPEECSTIRHWLIGFLNANESINANQFSLLARN
AIADIQSRGKIPFLLGGTGFYLRAFLLGMYPVPTVPKETKDYVFTLPLEEARSQLLAKDPKAMESLSDQDGYRIKRALEV
VLTGVLWSDVSKETVGGFWKDNPEVKIVGHWLDWPREILYQRINTRVETIIRGMLEETKEVLSKYGPDCPGLRTLGYNFA
LAFLNGMIDSNTFIEQLAQSHRNYAKRQITWFKKESFLSPISYDAAVQLYTNIEQR
>Mature_296_residues
MILPILGGPTGSGKTSLTQVLDPKRFEIVSFDSRQVYRDLPVGTTAPTPEECSTIRHWLIGFLNANESINANQFSLLARN
AIADIQSRGKIPFLLGGTGFYLRAFLLGMYPVPTVPKETKDYVFTLPLEEARSQLLAKDPKAMESLSDQDGYRIKRALEV
VLTGVLWSDVSKETVGGFWKDNPEVKIVGHWLDWPREILYQRINTRVETIIRGMLEETKEVLSKYGPDCPGLRTLGYNFA
LAFLNGMIDSNTFIEQLAQSHRNYAKRQITWFKKESFLSPISYDAAVQLYTNIEQR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=313, Percent_Identity=27.1565495207668, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1790613, Length=277, Percent_Identity=29.6028880866426, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25144712, Length=265, Percent_Identity=29.811320754717, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324848, Length=292, Percent_Identity=30.4794520547945, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24649830, Length=266, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_LEPBA (B0SBH8)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961577.1
- ProteinModelPortal:   B0SBH8
- SMR:   B0SBH8
- GeneID:   6387721
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0460
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   CLSK573952
- BioCyc:   LBIF355278:LBF_0460-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33515; Mature: 33515

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPILGGPTGSGKTSLTQVLDPKRFEIVSFDSRQVYRDLPVGTTAPTPEECSTIRHWLI
CEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHHH
GFLNANESINANQFSLLARNAIADIQSRGKIPFLLGGTGFYLRAFLLGMYPVPTVPKETK
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCC
DYVFTLPLEEARSQLLAKDPKAMESLSDQDGYRIKRALEVVLTGVLWSDVSKETVGGFWK
CEEEEECHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
DNPEVKIVGHWLDWPREILYQRINTRVETIIRGMLEETKEVLSKYGPDCPGLRTLGYNFA
CCCCEEEEECHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LAFLNGMIDSNTFIEQLAQSHRNYAKRQITWFKKESFLSPISYDAAVQLYTNIEQR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MILPILGGPTGSGKTSLTQVLDPKRFEIVSFDSRQVYRDLPVGTTAPTPEECSTIRHWLI
CEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHHH
GFLNANESINANQFSLLARNAIADIQSRGKIPFLLGGTGFYLRAFLLGMYPVPTVPKETK
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCC
DYVFTLPLEEARSQLLAKDPKAMESLSDQDGYRIKRALEVVLTGVLWSDVSKETVGGFWK
CEEEEECHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
DNPEVKIVGHWLDWPREILYQRINTRVETIIRGMLEETKEVLSKYGPDCPGLRTLGYNFA
CCCCEEEEECHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LAFLNGMIDSNTFIEQLAQSHRNYAKRQITWFKKESFLSPISYDAAVQLYTNIEQR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA