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The map label for this gene is rlmH1

Identifier: 18311582

GI number: 18311582

Start: 2978227

End: 2978706

Strand: Reverse

Name: rlmH1

Synonym: CPE2600

Alternate gene names: 18311582

Gene position: 2978706-2978227 (Counterclockwise)

Preceding gene: 18311583

Following gene: 18311581

Centisome position: 98.26

GC content: 27.5

Gene sequence:

>480_bases
ATGAATATAACAGTGATTTCAGTAGGGAAACTAAAGGAAAAGTATTTAAAGCAGGCTATAGATGAGTATTCAAAAAGATT
AAGTAGATATTGCAAATTAGAAATAATAGAACTTCCTGATGAGAAAACACCAGATAATGCTTCAGAAAAAGAGGAGCTTC
AAATAAAAGAAAAAGAAGGTAAGTTAATACTTTCAAAAATAAAGGATAATATGCATGTAATTGCTATGGATTTAAAAGGT
AATGAAATAACTTCTGAGAAATTTTCAAAGTATATAGAGAATTGTGGAGTTATGGGAAATTCAAATATTACCTTTGTAAT
TGGAGGAAGCCTTGGTTTATCACAAGAAGTAATAAAAAGAGCTGATTACAAATTATGCTTTTCAAAAATGACATTTCCCC
ATCAATTATTTAGAGTAATGTTATTAGAACAAGTTTATAGAGCATTTAGAATTATGAAGAATGAACCTTATCACAAATAA

Upstream 100 bases:

>100_bases
ATTATGAATTATTAATAAGAGGTATATGGTGTTTTATAGGATTACTAAGAGCTTTACTTTATGGTAATATTATTGTTAGT
TAAAACTAGGAGGGCGAAAG

Downstream 100 bases:

>100_bases
TGGATATTTTTACTTTAAATAGATTAAAATGAATTAAGAGAAATAGACTAAATAAAACTTAATATAAATGATAAACTAGT
TGAATATCTAGTAAAATAAG

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase 1; rRNA (pseudouridine-N3-)-methyltransferase rlmH 1

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNITVISVGKLKEKYLKQAIDEYSKRLSRYCKLEIIELPDEKTPDNASEKEELQIKEKEGKLILSKIKDNMHVIAMDLKG
NEITSEKFSKYIENCGVMGNSNITFVIGGSLGLSQEVIKRADYKLCFSKMTFPHQLFRVMLLEQVYRAFRIMKNEPYHK

Sequences:

>Translated_159_residues
MNITVISVGKLKEKYLKQAIDEYSKRLSRYCKLEIIELPDEKTPDNASEKEELQIKEKEGKLILSKIKDNMHVIAMDLKG
NEITSEKFSKYIENCGVMGNSNITFVIGGSLGLSQEVIKRADYKLCFSKMTFPHQLFRVMLLEQVYRAFRIMKNEPYHK
>Mature_159_residues
MNITVISVGKLKEKYLKQAIDEYSKRLSRYCKLEIIELPDEKTPDNASEKEELQIKEKEGKLILSKIKDNMHVIAMDLKG
NEITSEKFSKYIENCGVMGNSNITFVIGGSLGLSQEVIKRADYKLCFSKMTFPHQLFRVMLLEQVYRAFRIMKNEPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=29.559748427673, Blast_Score=90, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH1_CLOPS (Q0SPV0)

Other databases:

- EMBL:   CP000312
- RefSeq:   YP_699857.1
- ProteinModelPortal:   Q0SPV0
- SMR:   Q0SPV0
- STRING:   Q0SPV0
- GeneID:   4204251
- GenomeReviews:   CP000312_GR
- KEGG:   cpr:CPR_2610
- TIGR:   CPR_2610
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- BioCyc:   CPER289380:CPR_2610-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18473; Mature: 18473

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITVISVGKLKEKYLKQAIDEYSKRLSRYCKLEIIELPDEKTPDNASEKEELQIKEKEG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHHEECCCC
KLILSKIKDNMHVIAMDLKGNEITSEKFSKYIENCGVMGNSNITFVIGGSLGLSQEVIKR
CCHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHH
ADYKLCFSKMTFPHQLFRVMLLEQVYRAFRIMKNEPYHK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNITVISVGKLKEKYLKQAIDEYSKRLSRYCKLEIIELPDEKTPDNASEKEELQIKEKEG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHHEECCCC
KLILSKIKDNMHVIAMDLKGNEITSEKFSKYIENCGVMGNSNITFVIGGSLGLSQEVIKR
CCHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHH
ADYKLCFSKMTFPHQLFRVMLLEQVYRAFRIMKNEPYHK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA