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The map label for this gene is metG

Identifier: 18311506

GI number: 18311506

Start: 2889580

End: 2891517

Strand: Reverse

Name: metG

Synonym: CPE2524

Alternate gene names: 18311506

Gene position: 2891517-2889580 (Counterclockwise)

Preceding gene: 18311509

Following gene: 18311505

Centisome position: 95.38

GC content: 31.84

Gene sequence:

>1938_bases
ATGTGTAAAAAACCATATTATATTACAACGCCTATTTATTACCCATCAACAAATCTACACATAGGTAATACTTATACAAC
TGTAGCTGCTGATGCTATAGCAAGATTCAAAAGACTTACAGGTCACGAAGTGATGTTCTTAACAGGAACAGATGAGCATG
GTCAAAAAATAGAAAGAATAGCTAATGAAAAAGGAATTACTCCAAAAGAGCACGTTGACGAAATAGTTGCAGGGATTAAA
GATCTTTGGAAAATGATGAACATAAGCTATGACAAGTTTATAAGAACAACTGATGATTATCATGTGAAAGCTGTTCAAGA
GATTTTCAAAAAGTTATATGATCAAGGAGATATATATAAAGATTCTTATGAAGGTCTTTACTGTACTCCATGTGAATCAT
TCTGGACAGAAACTCAATTAGTAAATGGAAACTGTCCTGATTGTGGAAGACCAGTTGAAAAAGCTAAGGAAGAAGCTTAC
TTCTTTAAAATGAGCAAATATGCTGATAGATTAATTCAGTATATAGAAGAGCATCCAGATTTCATTCAACCAGAATCAAG
AAAGAATGAAATGTTAAATAACTTCTTAAGACCAGGATTACAAGATCTTTGTGTTTCAAGAACAAGCTTTACTTGGGGAA
TACCTGTAAGCTTTGATGAGAAGCATGTTATATATGTTTGGATAGATGCATTATCAAACTACATAACTGCTTTAGGATAT
GGTCAAGAAAACCAAGAGTTATACAAAAAGTTCTGGCCAGCTGATGTTCACTTAATAGGAAAAGATATTTTAAGATTCCA
TACAATTTACTGGCCAATAATGTTAATGGCTTTAGGATTAGAATTACCTAAGCAAGTATTTGGTCACGGATGGTTACTTG
TTGATGGAGGAAAAATGTCAAAATCAAAAGGTAACGTTGTTGATCCAGTAGTTTTAGTTAACATGTTTGGAGCTGACGCT
GTAAGATACTACTTATTAAGAGAAATACCATTTGGTTCAGATGGATTATTCAACAATGAAATATTTATAAAGAAAGTAAA
TACAGACTTAGCTAATGACTTAGGTAACTTATTATCAAGAACAATAGCTATGGTTTATAAATACTTTGGTGGAGTTATTC
AAGCACCAACATGTAAAGAACCTATTGATGATGAGTTAATAAACTTAGCTTTATCTACTCCAGGAAAAGTTGAAGCTAGC
ATAGATGCATTAAAGATTCCAGAAGCTTTAGAAAGTATATGGACTTTAATAAGCAGAGCTAACAAATATATAGATGAAAC
AACTCCTTGGATACTTGCTAAGGATGAAGAAAAGAAAGAAAGACTAGGAACAGTTTTATATAACTTATTAGAAACTTTAA
GATTTGTTTCAGTAATGATTTCACCATTCTTAACAGAAACAAGTGTAAAAATAAATGCTCAATTAAACACTAAAGTTACA
ACTTGGGAAAGTTTAAAAGAGTTTAATGGAACAGTTGCAGGAGATAAAGTAGTTAAGGGTGATGTTATATTCCCAAGAAT
AGATGTTGAGGAAAAATTAGCAGAACTTGAAGCTTTAAAACCAGCTCCAGTTAAACCAGCTAACGAAGAGTTAGTTGAAA
ATCCAATAAAAGAAGAAATAACTATAGATGACTTTGACAAGATAGATTTAAGAGTTGTTAAAGTATTAGAATGTGAACCA
GTTAAAAAAGCTAAAAAGTTATTAAAACTTAAGGTTGATTTAGGTGGAGAAGAAAGACAGGTTATTTCAGGAATAGCACA
ATACTATAAACCAGAAGAGTTAGTTGGAAAGTATGTAGTATTAGTTGCTAACTTAAAACCAGTTAAATTAAGAGGAGAAT
TATCTCAAGGTATGATACTTGCAGCAGCACCTTCAGATGATTCAGAACTTCTATTAGTTAACCCAGGAGAAATGTTAACT
GGAAGCCAAGTAAGATAG

Upstream 100 bases:

>100_bases
ATCGCGTAATTAACGCCTCTATGGGATTTCCCATAGAGGTTTTTTATATTTTTGAAATATTGGAAACAATAATAAGTAAG
AATACAAGGAGGAAGAAATA

Downstream 100 bases:

>100_bases
TAAAATTAAAAAACTCTTATATTCATACTAGATTGTGGAATATAAGAGTTTTTTTGTATCATAATATTGTAGATAGAAGC
ATTTTAAGGGGGCTAATAAA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 645; Mature: 645

Protein sequence:

>645_residues
MCKKPYYITTPIYYPSTNLHIGNTYTTVAADAIARFKRLTGHEVMFLTGTDEHGQKIERIANEKGITPKEHVDEIVAGIK
DLWKMMNISYDKFIRTTDDYHVKAVQEIFKKLYDQGDIYKDSYEGLYCTPCESFWTETQLVNGNCPDCGRPVEKAKEEAY
FFKMSKYADRLIQYIEEHPDFIQPESRKNEMLNNFLRPGLQDLCVSRTSFTWGIPVSFDEKHVIYVWIDALSNYITALGY
GQENQELYKKFWPADVHLIGKDILRFHTIYWPIMLMALGLELPKQVFGHGWLLVDGGKMSKSKGNVVDPVVLVNMFGADA
VRYYLLREIPFGSDGLFNNEIFIKKVNTDLANDLGNLLSRTIAMVYKYFGGVIQAPTCKEPIDDELINLALSTPGKVEAS
IDALKIPEALESIWTLISRANKYIDETTPWILAKDEEKKERLGTVLYNLLETLRFVSVMISPFLTETSVKINAQLNTKVT
TWESLKEFNGTVAGDKVVKGDVIFPRIDVEEKLAELEALKPAPVKPANEELVENPIKEEITIDDFDKIDLRVVKVLECEP
VKKAKKLLKLKVDLGGEERQVISGIAQYYKPEELVGKYVVLVANLKPVKLRGELSQGMILAAAPSDDSELLLVNPGEMLT
GSQVR

Sequences:

>Translated_645_residues
MCKKPYYITTPIYYPSTNLHIGNTYTTVAADAIARFKRLTGHEVMFLTGTDEHGQKIERIANEKGITPKEHVDEIVAGIK
DLWKMMNISYDKFIRTTDDYHVKAVQEIFKKLYDQGDIYKDSYEGLYCTPCESFWTETQLVNGNCPDCGRPVEKAKEEAY
FFKMSKYADRLIQYIEEHPDFIQPESRKNEMLNNFLRPGLQDLCVSRTSFTWGIPVSFDEKHVIYVWIDALSNYITALGY
GQENQELYKKFWPADVHLIGKDILRFHTIYWPIMLMALGLELPKQVFGHGWLLVDGGKMSKSKGNVVDPVVLVNMFGADA
VRYYLLREIPFGSDGLFNNEIFIKKVNTDLANDLGNLLSRTIAMVYKYFGGVIQAPTCKEPIDDELINLALSTPGKVEAS
IDALKIPEALESIWTLISRANKYIDETTPWILAKDEEKKERLGTVLYNLLETLRFVSVMISPFLTETSVKINAQLNTKVT
TWESLKEFNGTVAGDKVVKGDVIFPRIDVEEKLAELEALKPAPVKPANEELVENPIKEEITIDDFDKIDLRVVKVLECEP
VKKAKKLLKLKVDLGGEERQVISGIAQYYKPEELVGKYVVLVANLKPVKLRGELSQGMILAAAPSDDSELLLVNPGEMLT
GSQVR
>Mature_645_residues
MCKKPYYITTPIYYPSTNLHIGNTYTTVAADAIARFKRLTGHEVMFLTGTDEHGQKIERIANEKGITPKEHVDEIVAGIK
DLWKMMNISYDKFIRTTDDYHVKAVQEIFKKLYDQGDIYKDSYEGLYCTPCESFWTETQLVNGNCPDCGRPVEKAKEEAY
FFKMSKYADRLIQYIEEHPDFIQPESRKNEMLNNFLRPGLQDLCVSRTSFTWGIPVSFDEKHVIYVWIDALSNYITALGY
GQENQELYKKFWPADVHLIGKDILRFHTIYWPIMLMALGLELPKQVFGHGWLLVDGGKMSKSKGNVVDPVVLVNMFGADA
VRYYLLREIPFGSDGLFNNEIFIKKVNTDLANDLGNLLSRTIAMVYKYFGGVIQAPTCKEPIDDELINLALSTPGKVEAS
IDALKIPEALESIWTLISRANKYIDETTPWILAKDEEKKERLGTVLYNLLETLRFVSVMISPFLTETSVKINAQLNTKVT
TWESLKEFNGTVAGDKVVKGDVIFPRIDVEEKLAELEALKPAPVKPANEELVENPIKEEITIDDFDKIDLRVVKVLECEP
VKKAKKLLKLKVDLGGEERQVISGIAQYYKPEELVGKYVVLVANLKPVKLRGELSQGMILAAAPSDDSELLLVNPGEMLT
GSQVR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI24308436, Length=549, Percent_Identity=34.9726775956284, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI14043022, Length=511, Percent_Identity=29.5499021526419, Blast_Score=197, Evalue=3e-50,
Organism=Escherichia coli, GI1788432, Length=681, Percent_Identity=29.6622613803231, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1786861, Length=190, Percent_Identity=27.3684210526316, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1789455, Length=102, Percent_Identity=38.2352941176471, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI212645096, Length=478, Percent_Identity=34.3096234309623, Blast_Score=267, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17540794, Length=563, Percent_Identity=28.2415630550622, Blast_Score=195, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI71992781, Length=368, Percent_Identity=32.8804347826087, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI71997510, Length=168, Percent_Identity=31.547619047619, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71997517, Length=168, Percent_Identity=31.547619047619, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321610, Length=512, Percent_Identity=32.2265625, Blast_Score=258, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6321704, Length=566, Percent_Identity=27.0318021201413, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6323414, Length=179, Percent_Identity=29.0502793296089, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21358483, Length=557, Percent_Identity=31.5978456014363, Blast_Score=288, Evalue=6e-78,
Organism=Drosophila melanogaster, GI24655311, Length=558, Percent_Identity=26.1648745519713, Blast_Score=182, Evalue=8e-46,
Organism=Drosophila melanogaster, GI21355409, Length=175, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=6e-15,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_CLOPE (Q8XHG1)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_563440.1
- ProteinModelPortal:   Q8XHG1
- SMR:   Q8XHG1
- GeneID:   990901
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE2524
- NMPDR:   fig|195102.1.peg.2587
- HOGENOM:   HBG721667
- OMA:   APHIGHA
- PhylomeDB:   Q8XHG1
- ProtClustDB:   PRK12267
- BioCyc:   CPER195102:CPE2524-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_01228
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 73174; Mature: 73174

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 301-301

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCKKPYYITTPIYYPSTNLHIGNTYTTVAADAIARFKRLTGHEVMFLTGTDEHGQKIERI
CCCCCEEEECCEEECCCCEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
ANEKGITPKEHVDEIVAGIKDLWKMMNISYDKFIRTTDDYHVKAVQEIFKKLYDQGDIYK
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC
DSYEGLYCTPCESFWTETQLVNGNCPDCGRPVEKAKEEAYFFKMSKYADRLIQYIEEHPD
CCCCCEEECCCHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FIQPESRKNEMLNNFLRPGLQDLCVSRTSFTWGIPVSFDEKHVIYVWIDALSNYITALGY
CCCCCHHHHHHHHHHHCCCHHHHHHCCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHHCC
GQENQELYKKFWPADVHLIGKDILRFHTIYWPIMLMALGLELPKQVFGHGWLLVDGGKMS
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCEEEEECCCCC
KSKGNVVDPVVLVNMFGADAVRYYLLREIPFGSDGLFNNEIFIKKVNTDLANDLGNLLSR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHH
TIAMVYKYFGGVIQAPTCKEPIDDELINLALSTPGKVEASIDALKIPEALESIWTLISRA
HHHHHHHHHCCEECCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NKYIDETTPWILAKDEEKKERLGTVLYNLLETLRFVSVMISPFLTETSVKINAQLNTKVT
HHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEC
TWESLKEFNGTVAGDKVVKGDVIFPRIDVEEKLAELEALKPAPVKPANEELVENPIKEEI
CHHHHHHCCCCCCCCCEEECCEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCHHCCC
TIDDFDKIDLRVVKVLECEPVKKAKKLLKLKVDLGGEERQVISGIAQYYKPEELVGKYVV
CCCCCCCCCCEEEEHHCCCCHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCHHHHCCEEE
LVANLKPVKLRGELSQGMILAAAPSDDSELLLVNPGEMLTGSQVR
EEECCCCEEEECCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MCKKPYYITTPIYYPSTNLHIGNTYTTVAADAIARFKRLTGHEVMFLTGTDEHGQKIERI
CCCCCEEEECCEEECCCCEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
ANEKGITPKEHVDEIVAGIKDLWKMMNISYDKFIRTTDDYHVKAVQEIFKKLYDQGDIYK
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC
DSYEGLYCTPCESFWTETQLVNGNCPDCGRPVEKAKEEAYFFKMSKYADRLIQYIEEHPD
CCCCCEEECCCHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FIQPESRKNEMLNNFLRPGLQDLCVSRTSFTWGIPVSFDEKHVIYVWIDALSNYITALGY
CCCCCHHHHHHHHHHHCCCHHHHHHCCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHHCC
GQENQELYKKFWPADVHLIGKDILRFHTIYWPIMLMALGLELPKQVFGHGWLLVDGGKMS
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCEEEEECCCCC
KSKGNVVDPVVLVNMFGADAVRYYLLREIPFGSDGLFNNEIFIKKVNTDLANDLGNLLSR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHH
TIAMVYKYFGGVIQAPTCKEPIDDELINLALSTPGKVEASIDALKIPEALESIWTLISRA
HHHHHHHHHCCEECCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NKYIDETTPWILAKDEEKKERLGTVLYNLLETLRFVSVMISPFLTETSVKINAQLNTKVT
HHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEC
TWESLKEFNGTVAGDKVVKGDVIFPRIDVEEKLAELEALKPAPVKPANEELVENPIKEEI
CHHHHHHCCCCCCCCCEEECCEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCHHCCC
TIDDFDKIDLRVVKVLECEPVKKAKKLLKLKVDLGGEERQVISGIAQYYKPEELVGKYVV
CCCCCCCCCCEEEEHHCCCCHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCHHHHCCEEE
LVANLKPVKLRGELSQGMILAAAPSDDSELLLVNPGEMLTGSQVR
EEECCCCEEEECCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842