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The map label for this gene is metS [H]

Identifier: 18311505

GI number: 18311505

Start: 2887935

End: 2889479

Strand: Reverse

Name: metS [H]

Synonym: CPE2523

Alternate gene names: 18311505

Gene position: 2889479-2887935 (Counterclockwise)

Preceding gene: 18311506

Following gene: 18311503

Centisome position: 95.32

GC content: 27.9

Gene sequence:

>1545_bases
ATGAAAGTAATTATAGGTAATGCTTGGCCGTATGCCAATGGTTCACTTCATATAGGAAGAGTTTCATCTTGGATGGCAGG
AGATATATTAGCAAGATATCATAGGGCTAAAGGTGATGAAGTTATTTTTGTTTCAGGATCTGACTGTCATGGATCTCCAA
TTTTAAATAAAGCCAAGGAGATTAATAAGTCACCAGAAGAGCTTATAAATAAGTACCATAGGGAATTTGTTAGATGTTTT
AATAAATTAGGATTTTCCTTTAATATATTTACAAGAACAGATAGTGAGTATCATGAAAAGCAGGTGAAAAATATAATAAA
AAGCCTTTATAATAAGGGATTTATATATGAAAAAGAAACAGAAGAATTTTATTGTCCAAAATGCTGTGATACCTTAGATG
AATTTGGGATTAAAGAAGGACTTTGTAATGAATGTAATAGTAAAGTTGAAGTTAGAAGTTCTAATAATCTTTTCTTTAAA
TTATCTTATTTTCAAGATTATATTCAAAATATATTAGATGAAGAAGAGAGTTGGAGAGAAAATGCCATTAAAATAACTAA
GAGATATTTAGAGGGCGGGCTAAGAGATAAGATTCTAACAAGAGAAATTAATTGGGGAATAGAAGTTCCTATAGAAGGCT
TTGAAGATAAAAGAGTTTATGTTTGGATTGATGCACTGTTAGCCTATGTTACTGCATCTAAGAAAGTTATAGAGGAAAAA
GGACAATCTTTAGAAGAATATTGGAATAATGAAGATAGTAGAATATATTTAGTTCATGGTAAGGAAAATATACCCTTTCA
TACAACAATGTTTCCAGCAATTCTCTCTGGCATTGGATTAGAGAAAAGTGAGATTAGAATATTCTCTTCTCAATATTTAA
CCTTAGAAGGAAAAACTTTTTCTACAAATAGAAATTGGGCCATATGGGTTCCTTATATTATGGAAAGATATAATATAGAT
TCTATTAGATATTATTTAATAAGTAGAGGTGCTGAAGAGAAAAATTCTGACTTTACTTGGAGAGATTTTATAAATGCAAA
TAATAATGAATTAGTAGGAGAAATAGGAAACTTTACAAATAGGGTTTTAACCTTTATAAAGAAAAACTTTAGTGGGGAAA
TAAAGGGAGAAGAACTTCCTCTTCAGTGGAAAAACTTAATAAGAAGAACTTATGCTTCTGTAGGTAATAAATTTGAAAAT
GGAGAGTTCAAATCAGGAGTAAAAGAAATGTTTAATCTTATAAAACAAGGAAATGATTTCTTTGATAAAAATAAACCTTG
GATTTTAATAAATAATAACAAGAAAAAATGTGAAGAGGTACTGTATATTTGTTCTCAAATATTAGTAGCTTTAACTGATT
TAATGAATCCTATAATTCCTTTTACTTGTGAGAAAATAAAAATGTTTTTAGGTATAAAGGATACAAAGTGGAGCTTTAAA
GAGAGGAAGAATATAAGAGTTAATAGAGTTGAATTTTTATTTGATAAAATAGACAAGAAAGTTGCTATAGAAGAATTAAA
TAGATTAAAGAATAAAAAAATATAA

Upstream 100 bases:

>100_bases
TAAAATTAAAAAACTCTTATATTCATACTAGATTGTGGAATATAAGAGTTTTTTTGTATCATAATATTGTAGATAGAAGC
ATTTTAAGGGGGCTAATAAA

Downstream 100 bases:

>100_bases
ATAAAATAAGCCTTGCTTCTATTAATCTAGAAACAAGGCTTATTCTACATCTTAAGGTTTAAGGTTAGGAATTATTATAT
AGAAATTTTTTAAATTAATT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase 1; MetRS 1 [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKVIIGNAWPYANGSLHIGRVSSWMAGDILARYHRAKGDEVIFVSGSDCHGSPILNKAKEINKSPEELINKYHREFVRCF
NKLGFSFNIFTRTDSEYHEKQVKNIIKSLYNKGFIYEKETEEFYCPKCCDTLDEFGIKEGLCNECNSKVEVRSSNNLFFK
LSYFQDYIQNILDEEESWRENAIKITKRYLEGGLRDKILTREINWGIEVPIEGFEDKRVYVWIDALLAYVTASKKVIEEK
GQSLEEYWNNEDSRIYLVHGKENIPFHTTMFPAILSGIGLEKSEIRIFSSQYLTLEGKTFSTNRNWAIWVPYIMERYNID
SIRYYLISRGAEEKNSDFTWRDFINANNNELVGEIGNFTNRVLTFIKKNFSGEIKGEELPLQWKNLIRRTYASVGNKFEN
GEFKSGVKEMFNLIKQGNDFFDKNKPWILINNNKKKCEEVLYICSQILVALTDLMNPIIPFTCEKIKMFLGIKDTKWSFK
ERKNIRVNRVEFLFDKIDKKVAIEELNRLKNKKI

Sequences:

>Translated_514_residues
MKVIIGNAWPYANGSLHIGRVSSWMAGDILARYHRAKGDEVIFVSGSDCHGSPILNKAKEINKSPEELINKYHREFVRCF
NKLGFSFNIFTRTDSEYHEKQVKNIIKSLYNKGFIYEKETEEFYCPKCCDTLDEFGIKEGLCNECNSKVEVRSSNNLFFK
LSYFQDYIQNILDEEESWRENAIKITKRYLEGGLRDKILTREINWGIEVPIEGFEDKRVYVWIDALLAYVTASKKVIEEK
GQSLEEYWNNEDSRIYLVHGKENIPFHTTMFPAILSGIGLEKSEIRIFSSQYLTLEGKTFSTNRNWAIWVPYIMERYNID
SIRYYLISRGAEEKNSDFTWRDFINANNNELVGEIGNFTNRVLTFIKKNFSGEIKGEELPLQWKNLIRRTYASVGNKFEN
GEFKSGVKEMFNLIKQGNDFFDKNKPWILINNNKKKCEEVLYICSQILVALTDLMNPIIPFTCEKIKMFLGIKDTKWSFK
ERKNIRVNRVEFLFDKIDKKVAIEELNRLKNKKI
>Mature_514_residues
MKVIIGNAWPYANGSLHIGRVSSWMAGDILARYHRAKGDEVIFVSGSDCHGSPILNKAKEINKSPEELINKYHREFVRCF
NKLGFSFNIFTRTDSEYHEKQVKNIIKSLYNKGFIYEKETEEFYCPKCCDTLDEFGIKEGLCNECNSKVEVRSSNNLFFK
LSYFQDYIQNILDEEESWRENAIKITKRYLEGGLRDKILTREINWGIEVPIEGFEDKRVYVWIDALLAYVTASKKVIEEK
GQSLEEYWNNEDSRIYLVHGKENIPFHTTMFPAILSGIGLEKSEIRIFSSQYLTLEGKTFSTNRNWAIWVPYIMERYNID
SIRYYLISRGAEEKNSDFTWRDFINANNNELVGEIGNFTNRVLTFIKKNFSGEIKGEELPLQWKNLIRRTYASVGNKFEN
GEFKSGVKEMFNLIKQGNDFFDKNKPWILINNNKKKCEEVLYICSQILVALTDLMNPIIPFTCEKIKMFLGIKDTKWSFK
ERKNIRVNRVEFLFDKIDKKVAIEELNRLKNKKI

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=521, Percent_Identity=26.4875239923225, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI24308436, Length=509, Percent_Identity=23.9685658153242, Blast_Score=149, Evalue=6e-36,
Organism=Escherichia coli, GI1788432, Length=540, Percent_Identity=26.4814814814815, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1786861, Length=183, Percent_Identity=28.9617486338798, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17540794, Length=514, Percent_Identity=26.2645914396887, Blast_Score=224, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI212645096, Length=491, Percent_Identity=22.8105906313646, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71992781, Length=391, Percent_Identity=21.227621483376, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6321704, Length=547, Percent_Identity=27.6051188299817, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321610, Length=503, Percent_Identity=24.0556660039761, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24655311, Length=522, Percent_Identity=30.0766283524904, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI21358483, Length=502, Percent_Identity=23.3067729083665, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 60342; Mature: 60342

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIIGNAWPYANGSLHIGRVSSWMAGDILARYHRAKGDEVIFVSGSDCHGSPILNKAKE
CEEEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHH
INKSPEELINKYHREFVRCFNKLGFSFNIFTRTDSEYHEKQVKNIIKSLYNKGFIYEKET
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEECCC
EEFYCPKCCDTLDEFGIKEGLCNECNSKVEVRSSNNLFFKLSYFQDYIQNILDEEESWRE
CCCCCCHHHHHHHHCCCHHHHHHHCCCCEEEECCCCEEEEEHHHHHHHHHHHCCHHHHHH
NAIKITKRYLEGGLRDKILTREINWGIEVPIEGFEDKRVYVWIDALLAYVTASKKVIEEK
HHHHHHHHHHCCCCHHHHHHEECCCCEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
GQSLEEYWNNEDSRIYLVHGKENIPFHTTMFPAILSGIGLEKSEIRIFSSQYLTLEGKTF
CHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHEEEEECCEEEEECCEE
STNRNWAIWVPYIMERYNIDSIRYYLISRGAEEKNSDFTWRDFINANNNELVGEIGNFTN
ECCCCEEEEHHHHHHHCCCCHHEEHHHHCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHH
RVLTFIKKNFSGEIKGEELPLQWKNLIRRTYASVGNKFENGEFKSGVKEMFNLIKQGNDF
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
FDKNKPWILINNNKKKCEEVLYICSQILVALTDLMNPIIPFTCEKIKMFLGIKDTKWSFK
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHH
ERKNIRVNRVEFLFDKIDKKVAIEELNRLKNKKI
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVIIGNAWPYANGSLHIGRVSSWMAGDILARYHRAKGDEVIFVSGSDCHGSPILNKAKE
CEEEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHH
INKSPEELINKYHREFVRCFNKLGFSFNIFTRTDSEYHEKQVKNIIKSLYNKGFIYEKET
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEECCC
EEFYCPKCCDTLDEFGIKEGLCNECNSKVEVRSSNNLFFKLSYFQDYIQNILDEEESWRE
CCCCCCHHHHHHHHCCCHHHHHHHCCCCEEEECCCCEEEEEHHHHHHHHHHHCCHHHHHH
NAIKITKRYLEGGLRDKILTREINWGIEVPIEGFEDKRVYVWIDALLAYVTASKKVIEEK
HHHHHHHHHHCCCCHHHHHHEECCCCEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
GQSLEEYWNNEDSRIYLVHGKENIPFHTTMFPAILSGIGLEKSEIRIFSSQYLTLEGKTF
CHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHEEEEECCEEEEECCEE
STNRNWAIWVPYIMERYNIDSIRYYLISRGAEEKNSDFTWRDFINANNNELVGEIGNFTN
ECCCCEEEEHHHHHHHCCCCHHEEHHHHCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHH
RVLTFIKKNFSGEIKGEELPLQWKNLIRRTYASVGNKFENGEFKSGVKEMFNLIKQGNDF
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
FDKNKPWILINNNKKKCEEVLYICSQILVALTDLMNPIIPFTCEKIKMFLGIKDTKWSFK
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHH
ERKNIRVNRVEFLFDKIDKKVAIEELNRLKNKKI
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA