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The map label for this gene is punA [H]

Identifier: 18311477

GI number: 18311477

Start: 2857020

End: 2857838

Strand: Direct

Name: punA [H]

Synonym: CPE2495

Alternate gene names: 18311477

Gene position: 2857020-2857838 (Clockwise)

Preceding gene: 18311476

Following gene: 18311478

Centisome position: 94.25

GC content: 33.94

Gene sequence:

>819_bases
ATGGATTTATCAAACAAAATTAAAGCAGCTGCTGAGTACATAAAAGGTAAAAGTAAATATAACCCAACAATAGGATTAAT
ATTAGGATCAGGATTAGGAGCTATAGCTGATCAAATAGAAGATGCTGAATATTTCCCATACAACGAAATTCCAAACTTCC
CTGTATCTACAGTTGAAGGTCATGCTGGAAGATTAGTTATAGGAAAATTCCAAGGTAAAGAAGTTGTTGCAATGCAAGGA
AGATTCCACTACTACGAAGGTTACTCAATGCAAGAAGTTACTTGCCCAGTAAGAGTAATGAGATTATTAGGAGTTGAAAC
TTTAGTAGTTACTAACGCTGCTGGAGCAGTTAACAAAGATTATACTCCTGGAGATTTAATGATAATATCAGACCACTTAA
ATTTATCAGGATCAAATCCTTTAATAGGTAAAAACTTAAATGAATTCGGTACTAGATTCCCTGATATGTCAAATGCATAT
GATAAAGCTTTAAGAGCTCAAGTTAAAGATATAGCTAAAAATTTAGGAATAGAAGTAAGAGAAGGTGTATATGCTATGTT
TAGTGGCCCAACTTACGAAACTCCTGCAGAAGTAAGAATGGCTAGAATATTAGGAGCAGATGCAGTTGGTATGTCAACAG
TTCCTGAAGTTATAATAGCTAACCACAGTGGAATGAAAGTTATAGGTGTATCATGCATGACTAACATGGCTGCTGGTATC
TTAGAACAACCACTTAACCATGAAGAAGTTATGGAAACTTCAGCTAAAGTTAGAAAGACATTCATAGAGTTAATGACTAA
CATTATAAAAGAGATTTAA

Upstream 100 bases:

>100_bases
AGAATTTTATACATTTTAAAGTAAGCGTTACCACATACTAGAAATATTCCACAATTAAAATCATTACTAAAATAAAATTT
CATTAAAGAGGTGTAGAGTT

Downstream 100 bases:

>100_bases
TAAAAAGTTAAATTAATATATTTCTTAACTTTTGTTAAATATAAAATTAGTATAAAAACATAAGAAAAGGAGAATAAATG
TGGATAGGTTTATTGGTGTA

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MDLSNKIKAAAEYIKGKSKYNPTIGLILGSGLGAIADQIEDAEYFPYNEIPNFPVSTVEGHAGRLVIGKFQGKEVVAMQG
RFHYYEGYSMQEVTCPVRVMRLLGVETLVVTNAAGAVNKDYTPGDLMIISDHLNLSGSNPLIGKNLNEFGTRFPDMSNAY
DKALRAQVKDIAKNLGIEVREGVYAMFSGPTYETPAEVRMARILGADAVGMSTVPEVIIANHSGMKVIGVSCMTNMAAGI
LEQPLNHEEVMETSAKVRKTFIELMTNIIKEI

Sequences:

>Translated_272_residues
MDLSNKIKAAAEYIKGKSKYNPTIGLILGSGLGAIADQIEDAEYFPYNEIPNFPVSTVEGHAGRLVIGKFQGKEVVAMQG
RFHYYEGYSMQEVTCPVRVMRLLGVETLVVTNAAGAVNKDYTPGDLMIISDHLNLSGSNPLIGKNLNEFGTRFPDMSNAY
DKALRAQVKDIAKNLGIEVREGVYAMFSGPTYETPAEVRMARILGADAVGMSTVPEVIIANHSGMKVIGVSCMTNMAAGI
LEQPLNHEEVMETSAKVRKTFIELMTNIIKEI
>Mature_272_residues
MDLSNKIKAAAEYIKGKSKYNPTIGLILGSGLGAIADQIEDAEYFPYNEIPNFPVSTVEGHAGRLVIGKFQGKEVVAMQG
RFHYYEGYSMQEVTCPVRVMRLLGVETLVVTNAAGAVNKDYTPGDLMIISDHLNLSGSNPLIGKNLNEFGTRFPDMSNAY
DKALRAQVKDIAKNLGIEVREGVYAMFSGPTYETPAEVRMARILGADAVGMSTVPEVIIANHSGMKVIGVSCMTNMAAGI
LEQPLNHEEVMETSAKVRKTFIELMTNIIKEI

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=273, Percent_Identity=49.8168498168498, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI47132622, Length=205, Percent_Identity=26.3414634146341, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1788746, Length=256, Percent_Identity=45.3125, Blast_Score=244, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI17541190, Length=264, Percent_Identity=43.5606060606061, Blast_Score=221, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6323238, Length=262, Percent_Identity=45.0381679389313, Blast_Score=202, Evalue=5e-53,
Organism=Drosophila melanogaster, GI45552887, Length=275, Percent_Identity=49.0909090909091, Blast_Score=263, Evalue=7e-71,
Organism=Drosophila melanogaster, GI45552885, Length=275, Percent_Identity=49.0909090909091, Blast_Score=263, Evalue=8e-71,
Organism=Drosophila melanogaster, GI24656093, Length=275, Percent_Identity=49.0909090909091, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24656090, Length=275, Percent_Identity=49.0909090909091, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24762376, Length=239, Percent_Identity=38.9121338912134, Blast_Score=163, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29641; Mature: 29641

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSNKIKAAAEYIKGKSKYNPTIGLILGSGLGAIADQIEDAEYFPYNEIPNFPVSTVEG
CCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
HAGRLVIGKFQGKEVVAMQGRFHYYEGYSMQEVTCPVRVMRLLGVETLVVTNAAGAVNKD
CCCEEEEEECCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHCHHHEEEECCCCCCCCC
YTPGDLMIISDHLNLSGSNPLIGKNLNEFGTRFPDMSNAYDKALRAQVKDIAKNLGIEVR
CCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEC
EGVYAMFSGPTYETPAEVRMARILGADAVGMSTVPEVIIANHSGMKVIGVSCMTNMAAGI
CCEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHCCCEEEECCCCCEEEHHHHHHHHHHHH
LEQPLNHEEVMETSAKVRKTFIELMTNIIKEI
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDLSNKIKAAAEYIKGKSKYNPTIGLILGSGLGAIADQIEDAEYFPYNEIPNFPVSTVEG
CCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
HAGRLVIGKFQGKEVVAMQGRFHYYEGYSMQEVTCPVRVMRLLGVETLVVTNAAGAVNKD
CCCEEEEEECCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHHCHHHEEEECCCCCCCCC
YTPGDLMIISDHLNLSGSNPLIGKNLNEFGTRFPDMSNAYDKALRAQVKDIAKNLGIEVR
CCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEC
EGVYAMFSGPTYETPAEVRMARILGADAVGMSTVPEVIIANHSGMKVIGVSCMTNMAAGI
CCEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHCCCEEEECCCCCEEEHHHHHHHHHHHH
LEQPLNHEEVMETSAKVRKTFIELMTNIIKEI
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9058965 [H]