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The map label for this gene is pykA

Identifier: 18311131

GI number: 18311131

Start: 2460912

End: 2462336

Strand: Direct

Name: pykA

Synonym: CPE2149

Alternate gene names: 18311131

Gene position: 2460912-2462336 (Clockwise)

Preceding gene: 18311103

Following gene: 18311146

Centisome position: 81.18

GC content: 32.0

Gene sequence:

>1425_bases
ATGCAAAAAACAAAAATGATATTTACAATTGGTCCTTCAAGTGATTCAGAAGAAATTTTAAGAGAGTTCATAAGAATTGG
TATGAATGCAGCTAGATTAAACTTCTCACATGGAGACCATGCAAGTCATAAAGAAAAAATAGAACTTATTAAGAGACTTA
GAAAAGAAGAAAAATCAGCTACAGCTATCTTATTAGACATTAAGGGTCCTAAAATAAGAACATATAATTTTAAAAATGGC
GAAGCTGAATTAAAAAATGGTGATGAATTCACATTCTCTTGCGGAGATGAAATATTAGGTGATAACACTAAATGTTCAAT
CTCTTATAAAGAACTATATGAAGACATAAAACCTGGTGGATCAATACTTGTTGATGATGGACTTTTAGAATTTAAAGTAA
AGGAAGTCCGTGGAACTGATATAATATGTGAAGTTATTGAAGGAGGTACTATAAAAGACCATAAAGGTGTAAATGTACCT
AATGTACCAATCAAACTCCCTGCAGTAACTGAAAAGGATAGATCTGACTTAATATTTGGTTGCGAGATGGAAGTAGACTT
TGTAGCAGCTTCTTTCATAAGAAAACCAGAGGATGTACTTGAAGTTAGAGAAATTCTAGACTCTCATGGTGGAAAAGATA
TAAAAATAATATCAAAAATAGAAAGCCAAGAAGGTGTAGATAACATAAAAGAAATAATTAAAGTTACTGATGGTGTAATG
GTTGCTAGAGGTGATATGGGAGTAGAAATTCCTATAGAAAATGTACCTATAATTCAAAAAAATATAATTAAGAAATGTAA
TCAAGCAGGGAAAATAGTTATAACGGCAACTCAAATGTTAGATTCTATGATAAGAAATCCAAGACCTACTAGAGCTGAAG
CTTCTGACGTATGTAATGCCATATTTGATGGTACAGATGCCATAATGCTTAGTGGAGAAAGTGCTTCTGGTAGCTTCCCT
ATAGAGGCAGCAATGACAATGTCTAGAATAGCAAAAAAAGCAGAAGCTAACTTAGACTATAATTATCTTTTAAGAAGATT
AAAAGATCCAAATCCAAATCCCGATGCCTTTGCAGATGCCATAAGTTATTCAGCATCAAAAACAGCTAGCAAATTCCCTA
CAAAAGCTATAGTAGCAGCCACTCAGACTGGTTCAACTGCTAAGATATTATCAAAATATAAACCTAGCTGTCCAATAATA
GCTATTACTCCATATGAAAAAGTTAGAAGAAGCTTAGCTTTAAACTTTGGAATAATTTCTAAGAAATGTGCTTATTTTAA
TAGTACAGATGAAATTATAGAGGAAGCTAGAAAAGTAGCTAAAGAATTTGAAATAGCTGAAACTGGAGATAATATAATGG
TAGCTGCTGGATTCCCAACATCAATAACTGGTGGAACAAACATGCTAAAAATTGAAAAAATATAA

Upstream 100 bases:

>100_bases
AATATAATTAATTTAATAATTTATTCTATTATATCAAATATATCAAACTTTTAAAGCAATCACTAAACTATTTTAGTTTA
AACAAAATGAGGTGGTTTTT

Downstream 100 bases:

>100_bases
TTTAATATAAAAAGGATAGTACTAAAAGTACTATCCTTTTCTAATCTATAAAGCCTATAAATTCTTAATAAAATCTATTA
TTTAATTAAAACATTAATAG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MQKTKMIFTIGPSSDSEEILREFIRIGMNAARLNFSHGDHASHKEKIELIKRLRKEEKSATAILLDIKGPKIRTYNFKNG
EAELKNGDEFTFSCGDEILGDNTKCSISYKELYEDIKPGGSILVDDGLLEFKVKEVRGTDIICEVIEGGTIKDHKGVNVP
NVPIKLPAVTEKDRSDLIFGCEMEVDFVAASFIRKPEDVLEVREILDSHGGKDIKIISKIESQEGVDNIKEIIKVTDGVM
VARGDMGVEIPIENVPIIQKNIIKKCNQAGKIVITATQMLDSMIRNPRPTRAEASDVCNAIFDGTDAIMLSGESASGSFP
IEAAMTMSRIAKKAEANLDYNYLLRRLKDPNPNPDAFADAISYSASKTASKFPTKAIVAATQTGSTAKILSKYKPSCPII
AITPYEKVRRSLALNFGIISKKCAYFNSTDEIIEEARKVAKEFEIAETGDNIMVAAGFPTSITGGTNMLKIEKI

Sequences:

>Translated_474_residues
MQKTKMIFTIGPSSDSEEILREFIRIGMNAARLNFSHGDHASHKEKIELIKRLRKEEKSATAILLDIKGPKIRTYNFKNG
EAELKNGDEFTFSCGDEILGDNTKCSISYKELYEDIKPGGSILVDDGLLEFKVKEVRGTDIICEVIEGGTIKDHKGVNVP
NVPIKLPAVTEKDRSDLIFGCEMEVDFVAASFIRKPEDVLEVREILDSHGGKDIKIISKIESQEGVDNIKEIIKVTDGVM
VARGDMGVEIPIENVPIIQKNIIKKCNQAGKIVITATQMLDSMIRNPRPTRAEASDVCNAIFDGTDAIMLSGESASGSFP
IEAAMTMSRIAKKAEANLDYNYLLRRLKDPNPNPDAFADAISYSASKTASKFPTKAIVAATQTGSTAKILSKYKPSCPII
AITPYEKVRRSLALNFGIISKKCAYFNSTDEIIEEARKVAKEFEIAETGDNIMVAAGFPTSITGGTNMLKIEKI
>Mature_474_residues
MQKTKMIFTIGPSSDSEEILREFIRIGMNAARLNFSHGDHASHKEKIELIKRLRKEEKSATAILLDIKGPKIRTYNFKNG
EAELKNGDEFTFSCGDEILGDNTKCSISYKELYEDIKPGGSILVDDGLLEFKVKEVRGTDIICEVIEGGTIKDHKGVNVP
NVPIKLPAVTEKDRSDLIFGCEMEVDFVAASFIRKPEDVLEVREILDSHGGKDIKIISKIESQEGVDNIKEIIKVTDGVM
VARGDMGVEIPIENVPIIQKNIIKKCNQAGKIVITATQMLDSMIRNPRPTRAEASDVCNAIFDGTDAIMLSGESASGSFP
IEAAMTMSRIAKKAEANLDYNYLLRRLKDPNPNPDAFADAISYSASKTASKFPTKAIVAATQTGSTAKILSKYKPSCPII
AITPYEKVRRSLALNFGIISKKCAYFNSTDEIIEEARKVAKEFEIAETGDNIMVAAGFPTSITGGTNMLKIEKI

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family

Homologues:

Organism=Homo sapiens, GI33286422, Length=488, Percent_Identity=41.5983606557377, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI33286420, Length=488, Percent_Identity=41.5983606557377, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI33286418, Length=488, Percent_Identity=42.2131147540984, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=39.3877551020408, Blast_Score=331, Evalue=8e-91,
Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=39.3877551020408, Blast_Score=331, Evalue=1e-90,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=46.3667820069204, Blast_Score=223, Evalue=4e-58,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=46.3667820069204, Blast_Score=223, Evalue=4e-58,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=44.7257383966245, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=44.7257383966245, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=43.9814814814815, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1787965, Length=462, Percent_Identity=46.3203463203463, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI1788160, Length=479, Percent_Identity=37.160751565762, Blast_Score=287, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17544584, Length=483, Percent_Identity=40.7867494824017, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=41.1157024793388, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=41.1157024793388, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=41.1157024793388, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=41.1157024793388, Blast_Score=309, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6324923, Length=485, Percent_Identity=40.4123711340206, Blast_Score=332, Evalue=7e-92,
Organism=Saccharomyces cerevisiae, GI6319279, Length=496, Percent_Identity=41.1290322580645, Blast_Score=330, Evalue=3e-91,
Organism=Drosophila melanogaster, GI24648964, Length=483, Percent_Identity=42.6501035196687, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI28571814, Length=483, Percent_Identity=42.6501035196687, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24648966, Length=392, Percent_Identity=46.1734693877551, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24581235, Length=480, Percent_Identity=35.4166666666667, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24646914, Length=260, Percent_Identity=44.6153846153846, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): KPYK_CLOPE (Q46289)

Other databases:

- EMBL:   BA000016
- EMBL:   X86495
- RefSeq:   NP_563065.1
- ProteinModelPortal:   Q46289
- SMR:   Q46289
- GeneID:   990464
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE2149
- NMPDR:   fig|195102.1.peg.2212
- HOGENOM:   HBG734376
- OMA:   HASHKEK
- PhylomeDB:   Q46289
- ProtClustDB:   CLSK811177
- BioCyc:   CPER195102:CPE2149-MONOMER
- BRENDA:   2.7.1.40
- GO:   GO:0006096
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806
- Gene3D:   G3DSA:2.40.33.10
- Gene3D:   G3DSA:3.20.20.60
- Gene3D:   G3DSA:3.40.1380.20
- PANTHER:   PTHR11817
- PRINTS:   PR01050
- TIGRFAMs:   TIGR01064

Pfam domain/function: PF00224 PK; PF02887 PK_C; SSF50800 PK_B_barrel_like; SSF52935 Pyruvate_kinase; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.1.40

Molecular weight: Translated: 52083; Mature: 52083

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: BINDING 32-32 BINDING 244-244 BINDING 245-245 BINDING 277-277

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTKMIFTIGPSSDSEEILREFIRIGMNAARLNFSHGDHASHKEKIELIKRLRKEEKSA
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
TAILLDIKGPKIRTYNFKNGEAELKNGDEFTFSCGDEILGDNTKCSISYKELYEDIKPGG
EEEEEECCCCEEEEEECCCCCCCCCCCCEEEEECCHHHHCCCCEEEEEHHHHHHHHCCCC
SILVDDGLLEFKVKEVRGTDIICEVIEGGTIKDHKGVNVPNVPIKLPAVTEKDRSDLIFG
CEEEECCCEEEEEEECCCCEEEEHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEE
CEMEVDFVAASFIRKPEDVLEVREILDSHGGKDIKIISKIESQEGVDNIKEIIKVTDGVM
ECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHCCHHHHHHHHHHHCCEE
VARGDMGVEIPIENVPIIQKNIIKKCNQAGKIVITATQMLDSMIRNPRPTRAEASDVCNA
EEECCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCHHHHHHH
IFDGTDAIMLSGESASGSFPIEAAMTMSRIAKKAEANLDYNYLLRRLKDPNPNPDAFADA
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHH
ISYSASKTASKFPTKAIVAATQTGSTAKILSKYKPSCPIIAITPYEKVRRSLALNFGIIS
HHHCHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCHH
KKCAYFNSTDEIIEEARKVAKEFEIAETGDNIMVAAGFPTSITGGTNMLKIEKI
HHHHCCCCHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MQKTKMIFTIGPSSDSEEILREFIRIGMNAARLNFSHGDHASHKEKIELIKRLRKEEKSA
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
TAILLDIKGPKIRTYNFKNGEAELKNGDEFTFSCGDEILGDNTKCSISYKELYEDIKPGG
EEEEEECCCCEEEEEECCCCCCCCCCCCEEEEECCHHHHCCCCEEEEEHHHHHHHHCCCC
SILVDDGLLEFKVKEVRGTDIICEVIEGGTIKDHKGVNVPNVPIKLPAVTEKDRSDLIFG
CEEEECCCEEEEEEECCCCEEEEHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEE
CEMEVDFVAASFIRKPEDVLEVREILDSHGGKDIKIISKIESQEGVDNIKEIIKVTDGVM
ECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHCCHHHHHHHHHHHCCEE
VARGDMGVEIPIENVPIIQKNIIKKCNQAGKIVITATQMLDSMIRNPRPTRAEASDVCNA
EEECCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCHHHHHHH
IFDGTDAIMLSGESASGSFPIEAAMTMSRIAKKAEANLDYNYLLRRLKDPNPNPDAFADA
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHH
ISYSASKTASKFPTKAIVAATQTGSTAKILSKYKPSCPIIAITPYEKVRRSLALNFGIIS
HHHCHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCHH
KKCAYFNSTDEIIEEARKVAKEFEIAETGDNIMVAAGFPTSITGGTNMLKIEKI
HHHHCCCCHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842; 7559358