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The map label for this gene is dacF [H]

Identifier: 18311103

GI number: 18311103

Start: 2433177

End: 2434511

Strand: Direct

Name: dacF [H]

Synonym: CPE2121

Alternate gene names: 18311103

Gene position: 2433177-2434511 (Clockwise)

Preceding gene: 18311101

Following gene: 18311131

Centisome position: 80.26

GC content: 27.72

Gene sequence:

>1335_bases
ATGAGTATAAAGTTTAAGAATTTAAAAATAAGACTTAATTTTATAATATCTATGGCTTTTATCTTCTCTTTAATTTTAAG
TACTAATGTTTTTGCTGAAAGTAATGTACCGGAAATTAAAGGTGAAGGAATGGTTCTTATGGATGGGACTACAGGTGAGG
TTTTAGCTGAAAAAAATGCTAATGAAAAACTTGCTCCTGCATCTACTACTAAAACTATGACAGCTTTACTTACAGTAGAA
AAAGCTAACTTAAATGATGTTGTTACTATAGGAAAGAATCCTCCTTTAGCTGAAGGAACCCGTGTTGGTCTTCAAGAAGG
AGATAAATATACAGTTGAAGAATTGCTTAATGCATTATTAATACAATCAGCTAATGATGCTGCTGTAGCTTTAGCTGAAC
ATATTTCTGGCTCTGAAGAAAAATTTGCAAAGCTTATGAATGAGCGTGCAGAAGAATTAGGAGCTAAAAATACTCACTTT
GTTAATGCTAGTGGACTTTTTGAGGATGATCATATGACTACCCCTTATGATCTAGCTCTAATAATGAATGCAGCTTCAAA
AAATCCAATAATTGATGAAATTACAAAAAAAACAGTTTATGAAATGCCTAAAAGCATTGTAAGTGGAGAACCTATATGGG
CAAATAATAATAATCAACTTATTCACCAAACAAGCCCATATTATAATAAAGATATTTTTTGTAACAAAACAGGATATACA
ACTAAATCAAGGCATACATTTACTTGTGCAGCTAAAAGGGGTGATAGAAAATTAATCCTTACCCTACTAGGTTATGACAC
TAAGGATCAATACTATGAAGATACAAAAAATCTTTTAGATTATGGCTTTGATAATTTTTCTTTAGTTAAATTATATTCTA
AAAATGATGTTGTAAAAGAATATTACATTGATGATAAGAACACCTTATCTTTAGTAGTTCCTGAAGATATTTATAAAACA
TTTAAGAACTCTGAAATAGAGAACTTATCTACAGATGAAATAAAGAAAAATTTAAACATAGACTTTTCAGTAAAAGCAAC
TAAACCTATATCTAGGGGTACTGACATATTAAAAGATAATGATTCTTTTACTGGAACACTTATGATTGGAAATACTCCTT
ATAAAACCTTAAATCTTTTAAACAGTAATGATATAACTTATACAAGTTTTGAAAAACTTATGTTTAAATTAAAGGGTTTT
AGTGGATTTTACATAGCTTTAGGTGTTATAGGTTTAATATTTTTAATAATCTTAATACTATTTATGATTAGACTTATATC
AACTATAAAAAGAATAAGAAATAGAAAAAAACGTAATTCATATTTTTATAATTAA

Upstream 100 bases:

>100_bases
CTCCATTTTTGAGTACCAAATTAATACAAATTTATAGAAAATATACTCTATTATTGTTATAATAATTATTGCAATTAAAA
TTAAAAATTTTGGAGGCTCT

Downstream 100 bases:

>100_bases
AAAACAAGTAGTTTTAAGCCAATGCTTAAAACTACTTGTTTTTTAATTTATTTATTAACTCTTTCATATCCTCTGGAAGA
TCTGCCCTTAAGCTTAAAGG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSIKFKNLKIRLNFIISMAFIFSLILSTNVFAESNVPEIKGEGMVLMDGTTGEVLAEKNANEKLAPASTTKTMTALLTVE
KANLNDVVTIGKNPPLAEGTRVGLQEGDKYTVEELLNALLIQSANDAAVALAEHISGSEEKFAKLMNERAEELGAKNTHF
VNASGLFEDDHMTTPYDLALIMNAASKNPIIDEITKKTVYEMPKSIVSGEPIWANNNNQLIHQTSPYYNKDIFCNKTGYT
TKSRHTFTCAAKRGDRKLILTLLGYDTKDQYYEDTKNLLDYGFDNFSLVKLYSKNDVVKEYYIDDKNTLSLVVPEDIYKT
FKNSEIENLSTDEIKKNLNIDFSVKATKPISRGTDILKDNDSFTGTLMIGNTPYKTLNLLNSNDITYTSFEKLMFKLKGF
SGFYIALGVIGLIFLIILILFMIRLISTIKRIRNRKKRNSYFYN

Sequences:

>Translated_444_residues
MSIKFKNLKIRLNFIISMAFIFSLILSTNVFAESNVPEIKGEGMVLMDGTTGEVLAEKNANEKLAPASTTKTMTALLTVE
KANLNDVVTIGKNPPLAEGTRVGLQEGDKYTVEELLNALLIQSANDAAVALAEHISGSEEKFAKLMNERAEELGAKNTHF
VNASGLFEDDHMTTPYDLALIMNAASKNPIIDEITKKTVYEMPKSIVSGEPIWANNNNQLIHQTSPYYNKDIFCNKTGYT
TKSRHTFTCAAKRGDRKLILTLLGYDTKDQYYEDTKNLLDYGFDNFSLVKLYSKNDVVKEYYIDDKNTLSLVVPEDIYKT
FKNSEIENLSTDEIKKNLNIDFSVKATKPISRGTDILKDNDSFTGTLMIGNTPYKTLNLLNSNDITYTSFEKLMFKLKGF
SGFYIALGVIGLIFLIILILFMIRLISTIKRIRNRKKRNSYFYN
>Mature_443_residues
SIKFKNLKIRLNFIISMAFIFSLILSTNVFAESNVPEIKGEGMVLMDGTTGEVLAEKNANEKLAPASTTKTMTALLTVEK
ANLNDVVTIGKNPPLAEGTRVGLQEGDKYTVEELLNALLIQSANDAAVALAEHISGSEEKFAKLMNERAEELGAKNTHFV
NASGLFEDDHMTTPYDLALIMNAASKNPIIDEITKKTVYEMPKSIVSGEPIWANNNNQLIHQTSPYYNKDIFCNKTGYTT
KSRHTFTCAAKRGDRKLILTLLGYDTKDQYYEDTKNLLDYGFDNFSLVKLYSKNDVVKEYYIDDKNTLSLVVPEDIYKTF
KNSEIENLSTDEIKKNLNIDFSVKATKPISRGTDILKDNDSFTGTLMIGNTPYKTLNLLNSNDITYTSFEKLMFKLKGFS
GFYIALGVIGLIFLIILILFMIRLISTIKRIRNRKKRNSYFYN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=326, Percent_Identity=32.8220858895706, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI87082022, Length=366, Percent_Identity=31.1475409836066, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1786851, Length=308, Percent_Identity=32.1428571428571, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI87082054, Length=290, Percent_Identity=23.1034482758621, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 50026; Mature: 49895

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKFKNLKIRLNFIISMAFIFSLILSTNVFAESNVPEIKGEGMVLMDGTTGEVLAEKNA
CCEEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCC
NEKLAPASTTKTMTALLTVEKANLNDVVTIGKNPPLAEGTRVGLQEGDKYTVEELLNALL
CCCCCCCCHHHHEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
IQSANDAAVALAEHISGSEEKFAKLMNERAEELGAKNTHFVNASGLFEDDHMTTPYDLAL
HCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHE
IMNAASKNPIIDEITKKTVYEMPKSIVSGEPIWANNNNQLIHQTSPYYNKDIFCNKTGYT
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCCEEECCCCCC
TKSRHTFTCAAKRGDRKLILTLLGYDTKDQYYEDTKNLLDYGFDNFSLVKLYSKNDVVKE
CCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHH
YYIDDKNTLSLVVPEDIYKTFKNSEIENLSTDEIKKNLNIDFSVKATKPISRGTDILKDN
EEECCCCEEEEEECHHHHHHHCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCEECC
DSFTGTLMIGNTPYKTLNLLNSNDITYTSFEKLMFKLKGFSGFYIALGVIGLIFLIILIL
CCEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
FMIRLISTIKRIRNRKKRNSYFYN
HHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SIKFKNLKIRLNFIISMAFIFSLILSTNVFAESNVPEIKGEGMVLMDGTTGEVLAEKNA
CEEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCC
NEKLAPASTTKTMTALLTVEKANLNDVVTIGKNPPLAEGTRVGLQEGDKYTVEELLNALL
CCCCCCCCHHHHEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
IQSANDAAVALAEHISGSEEKFAKLMNERAEELGAKNTHFVNASGLFEDDHMTTPYDLAL
HCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHE
IMNAASKNPIIDEITKKTVYEMPKSIVSGEPIWANNNNQLIHQTSPYYNKDIFCNKTGYT
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCCEEECCCCCC
TKSRHTFTCAAKRGDRKLILTLLGYDTKDQYYEDTKNLLDYGFDNFSLVKLYSKNDVVKE
CCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHH
YYIDDKNTLSLVVPEDIYKTFKNSEIENLSTDEIKKNLNIDFSVKATKPISRGTDILKDN
EEECCCCEEEEEECHHHHHHHCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCEECC
DSFTGTLMIGNTPYKTLNLLNSNDITYTSFEKLMFKLKGFSGFYIALGVIGLIFLIILIL
CCEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
FMIRLISTIKRIRNRKKRNSYFYN
HHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]