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The map label for this gene is polA [H]

Identifier: 18310976

GI number: 18310976

Start: 2283149

End: 2285749

Strand: Reverse

Name: polA [H]

Synonym: CPE1994

Alternate gene names: 18310976

Gene position: 2285749-2283149 (Counterclockwise)

Preceding gene: 18310977

Following gene: 18310975

Centisome position: 75.4

GC content: 27.87

Gene sequence:

>2601_bases
ATGAAAAGATTATTGATATTAGATGGGAACAGTCTTTTAAATAGAGCCTTTTATGCTGTGCCTTTGCTTAGCAATAGTGA
AGGAACTCATACAAATGCTATTTATGGTTTTACAAATATGCTTTTTAAGATGAGAGAGGATATAAAACCTGATTATATAG
TTTGTACTTTTGATAAAAAAGCACCAACTTTTAGACATTTAGAGTATGGGGATTATAAGGCTGGAAGAAAGAAAATGCCT
CCTGAGTTAGCAGAGCAGTTTCCTATTGTTAAGGAAATGTTAAAGTTATTTTCAATAACAACTTACGAATTAGATGGATT
CGAAGCAGATGATATTATAGGAAGTTTAGCTAAGGTGGCTGAAGGTGAAGGAATCGAAGTGTATATTATGACTGGAGATA
AGGATGCCCTTCAACTAGCAGCTAAGGATATAAACGTATGTATAACTAAGAAGGGTGTTTCTGAAATAGAAGTTTATAAC
TATGATAGAATGGTTGAAGAATATGGAGTTACTCCTACTCAGTTTATAGATGTTAAAGGACTTATGGGAGATAAATCTGA
TAATATTCCAGGGGTACCAGGAGTAGGAGAAAAAACAGCCTTTAAATTAATAAAAGAGTATGGTTCAGTAGAGGCAGTTT
TAGAAAATATAGATAACATAAGTGGTAAAAAGTTAAAAGAAAATTTAACTGAGTACAGCGAACAGGCTATATTCTCTAAA
AAGTTAGCTACTATTATGACTAATGTTCCTATAGATTTTGATTTAGATGAGATTAAATCAAAGGATGATTATAATAAAGA
TCAGTTAAAAGCTTTATTTACAAAGCTTCAAATGAAATCTATATTAGATAAATTATCAAATGGGGAAGAAGAAGAGCCAG
AAATAGAAGTTAAAATAACTAATATAGAAAATAATAAAGAATTAGAAGATTTATTTAAGAATATAAACGAAGAAGTATAT
ATTAATTTTACTTACTTAAATAAAGAAAAGTATTCTACTATAGAGATTGATAAAATATTTATTAATTATGATGAGAAAAA
TTATTTAATTAACTTTAAAGAGTTAGTAAAAGAAGAGGAGAATATTAAATCCTTTAAAGAATTTTTTGAGAATGAAGCTA
TATTAAAAAATGGCTATAGTATAAAAAATGCCTTAACAGCATTAAAAAAATTAGGAATAGATTTTAATGGATTAGCCTTT
GATTGTGAATTAGCAGCATATTTAATAGATTCATCTAAGGGAGAATATACTCTTTTATCCTTAATAAATAACTATCTTTC
AAAATTCCCTAATGTAAAAGAGGGAGAGGAAGACTTAGTTGGAATTACATTTATGAAAGAGTTGACTTTAGTTCTAAAGA
AAAAAATAGAAGAAGAGAAAATGGAAAGACTTATGTATGATATTGAGCTTCCTTTAGAAACAGTTTTATCTTCTATGGAA
TCAATAGGATTTAAAGTAAATAAAGATATTTTAGATGAGTTATCTATTAAGTTTAAAAGAGAGCTAGAAGAAACACAAAA
AGAGATCTTTGAACTTGCAGATGAAGAGTTCAACATAAATTCTCCAAAACAATTAGGTAAGATATTATTTGAAAAATTAG
ATTTACCAGTTATAAAGAAAACTAAAACTGGCTATTCAACTAACGCAGAAGTTTTAGAAAAGCTTAAGGACAAACATCCT
ATAATAGAAAAAATAACATATTTTAGACAGCTTTCAAAAATATATTCAACTTATGTTGAAGGATTAAAGGCTGTTATAGA
TACAGATGGTTCTATACATTCAACATTTAATCAAACTGTAACTACTACAGGTAGACTTTCATCAACAGAGCCTAACCTTC
AAAATATACCTATAAAATACGAAATGGGTAGAGAAATAAGAAAGGTATTTATACCAAAAAAAGAAGGAGATTTATTAATA
TCAGCTGACTACTCACAAATAGAACTTAGAGTTTTAGCTCATATTTCTGGTGATGAAAATATGATAGATGCATTTAATCA
TCATTCAGATATACACACTAAGACTGCTTCTGAAGTGTTTAAAGTGCCAGTTGAAGAGGTAACTCCATTAATGAGAAGTA
GGGCTAAGGCAGTAAACTTTGGTATAGTATATGGAATAGGAGATTTTAGTCTTTCTCAGGATTTAAATATTTCAAGAAAA
GAAGCTAAAGAATATATGGATATATATTTTGAAAGATATCCAAAAATTAAAGAGTATATTTCTAAGGTTATTCATGAAGC
TCAAACAAAAGGATATGTATTAACTATGTTAAATAGAAGAAGATATATACCTGAAGCAAATGCTTCAAATAAAATAGTAA
AAGCTTTAGGAGAAAGACTTGCAATGAATGCTCCAATACAAGGAACAGCAGCAGATATAATAAAGCTTGCTATGGTAAAT
GTATATAACAAGTTAAAAGAATTAAATTTAAATAGTAAGTTAATATTACAGGTTCACGATGAACTTATAATAAATGTTGT
AAAAGGAGAATTAGATATTGTTAAGAAACTTCTAAAAGAGGAAATGGAAAATACAATTAAATTAAGTGTACCATTAGATG
TTGATGTTAATGTTGGAGAAGATTGGTATAGGTTAAAATAA

Upstream 100 bases:

>100_bases
AATTCTTTGAATATATTTATAAGTTTTATTATAGAAGACTTTATATAATATAGTAGATAACTAGAATGTTAGATTAATAG
AAATATATTGGAGGATTAGA

Downstream 100 bases:

>100_bases
GGAGGGTACTGATTCTATGATTAAAGTAGGCCTTACAGGAGGAATATGCTCAGGTAAAAGCACTATTTCTTCTATGATAA
AAGAGGCAGGAATCCCAGTT

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 866; Mature: 866

Protein sequence:

>866_residues
MKRLLILDGNSLLNRAFYAVPLLSNSEGTHTNAIYGFTNMLFKMREDIKPDYIVCTFDKKAPTFRHLEYGDYKAGRKKMP
PELAEQFPIVKEMLKLFSITTYELDGFEADDIIGSLAKVAEGEGIEVYIMTGDKDALQLAAKDINVCITKKGVSEIEVYN
YDRMVEEYGVTPTQFIDVKGLMGDKSDNIPGVPGVGEKTAFKLIKEYGSVEAVLENIDNISGKKLKENLTEYSEQAIFSK
KLATIMTNVPIDFDLDEIKSKDDYNKDQLKALFTKLQMKSILDKLSNGEEEEPEIEVKITNIENNKELEDLFKNINEEVY
INFTYLNKEKYSTIEIDKIFINYDEKNYLINFKELVKEEENIKSFKEFFENEAILKNGYSIKNALTALKKLGIDFNGLAF
DCELAAYLIDSSKGEYTLLSLINNYLSKFPNVKEGEEDLVGITFMKELTLVLKKKIEEEKMERLMYDIELPLETVLSSME
SIGFKVNKDILDELSIKFKRELEETQKEIFELADEEFNINSPKQLGKILFEKLDLPVIKKTKTGYSTNAEVLEKLKDKHP
IIEKITYFRQLSKIYSTYVEGLKAVIDTDGSIHSTFNQTVTTTGRLSSTEPNLQNIPIKYEMGREIRKVFIPKKEGDLLI
SADYSQIELRVLAHISGDENMIDAFNHHSDIHTKTASEVFKVPVEEVTPLMRSRAKAVNFGIVYGIGDFSLSQDLNISRK
EAKEYMDIYFERYPKIKEYISKVIHEAQTKGYVLTMLNRRRYIPEANASNKIVKALGERLAMNAPIQGTAADIIKLAMVN
VYNKLKELNLNSKLILQVHDELIINVVKGELDIVKKLLKEEMENTIKLSVPLDVDVNVGEDWYRLK

Sequences:

>Translated_866_residues
MKRLLILDGNSLLNRAFYAVPLLSNSEGTHTNAIYGFTNMLFKMREDIKPDYIVCTFDKKAPTFRHLEYGDYKAGRKKMP
PELAEQFPIVKEMLKLFSITTYELDGFEADDIIGSLAKVAEGEGIEVYIMTGDKDALQLAAKDINVCITKKGVSEIEVYN
YDRMVEEYGVTPTQFIDVKGLMGDKSDNIPGVPGVGEKTAFKLIKEYGSVEAVLENIDNISGKKLKENLTEYSEQAIFSK
KLATIMTNVPIDFDLDEIKSKDDYNKDQLKALFTKLQMKSILDKLSNGEEEEPEIEVKITNIENNKELEDLFKNINEEVY
INFTYLNKEKYSTIEIDKIFINYDEKNYLINFKELVKEEENIKSFKEFFENEAILKNGYSIKNALTALKKLGIDFNGLAF
DCELAAYLIDSSKGEYTLLSLINNYLSKFPNVKEGEEDLVGITFMKELTLVLKKKIEEEKMERLMYDIELPLETVLSSME
SIGFKVNKDILDELSIKFKRELEETQKEIFELADEEFNINSPKQLGKILFEKLDLPVIKKTKTGYSTNAEVLEKLKDKHP
IIEKITYFRQLSKIYSTYVEGLKAVIDTDGSIHSTFNQTVTTTGRLSSTEPNLQNIPIKYEMGREIRKVFIPKKEGDLLI
SADYSQIELRVLAHISGDENMIDAFNHHSDIHTKTASEVFKVPVEEVTPLMRSRAKAVNFGIVYGIGDFSLSQDLNISRK
EAKEYMDIYFERYPKIKEYISKVIHEAQTKGYVLTMLNRRRYIPEANASNKIVKALGERLAMNAPIQGTAADIIKLAMVN
VYNKLKELNLNSKLILQVHDELIINVVKGELDIVKKLLKEEMENTIKLSVPLDVDVNVGEDWYRLK
>Mature_866_residues
MKRLLILDGNSLLNRAFYAVPLLSNSEGTHTNAIYGFTNMLFKMREDIKPDYIVCTFDKKAPTFRHLEYGDYKAGRKKMP
PELAEQFPIVKEMLKLFSITTYELDGFEADDIIGSLAKVAEGEGIEVYIMTGDKDALQLAAKDINVCITKKGVSEIEVYN
YDRMVEEYGVTPTQFIDVKGLMGDKSDNIPGVPGVGEKTAFKLIKEYGSVEAVLENIDNISGKKLKENLTEYSEQAIFSK
KLATIMTNVPIDFDLDEIKSKDDYNKDQLKALFTKLQMKSILDKLSNGEEEEPEIEVKITNIENNKELEDLFKNINEEVY
INFTYLNKEKYSTIEIDKIFINYDEKNYLINFKELVKEEENIKSFKEFFENEAILKNGYSIKNALTALKKLGIDFNGLAF
DCELAAYLIDSSKGEYTLLSLINNYLSKFPNVKEGEEDLVGITFMKELTLVLKKKIEEEKMERLMYDIELPLETVLSSME
SIGFKVNKDILDELSIKFKRELEETQKEIFELADEEFNINSPKQLGKILFEKLDLPVIKKTKTGYSTNAEVLEKLKDKHP
IIEKITYFRQLSKIYSTYVEGLKAVIDTDGSIHSTFNQTVTTTGRLSSTEPNLQNIPIKYEMGREIRKVFIPKKEGDLLI
SADYSQIELRVLAHISGDENMIDAFNHHSDIHTKTASEVFKVPVEEVTPLMRSRAKAVNFGIVYGIGDFSLSQDLNISRK
EAKEYMDIYFERYPKIKEYISKVIHEAQTKGYVLTMLNRRRYIPEANASNKIVKALGERLAMNAPIQGTAADIIKLAMVN
VYNKLKELNLNSKLILQVHDELIINVVKGELDIVKKLLKEEMENTIKLSVPLDVDVNVGEDWYRLK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=517, Percent_Identity=34.0425531914894, Blast_Score=271, Evalue=2e-72,
Organism=Homo sapiens, GI139394648, Length=615, Percent_Identity=26.9918699186992, Blast_Score=208, Evalue=1e-53,
Organism=Escherichia coli, GI1790294, Length=932, Percent_Identity=36.587982832618, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI226510970, Length=213, Percent_Identity=29.5774647887324, Blast_Score=111, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI86563272, Length=403, Percent_Identity=30.0248138957816, Blast_Score=185, Evalue=1e-46,
Organism=Drosophila melanogaster, GI17933644, Length=483, Percent_Identity=32.9192546583851, Blast_Score=246, Evalue=4e-65,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 99119; Mature: 99119

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLLILDGNSLLNRAFYAVPLLSNSEGTHTNAIYGFTNMLFKMREDIKPDYIVCTFDKK
CCEEEEECCCHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCC
APTFRHLEYGDYKAGRKKMPPELAEQFPIVKEMLKLFSITTYELDGFEADDIIGSLAKVA
CCCEECCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHH
EGEGIEVYIMTGDKDALQLAAKDINVCITKKGVSEIEVYNYDRMVEEYGVTPTQFIDVKG
CCCCEEEEEEECCHHHHHHHHCCCEEEEEECCCCCEEEECHHHHHHHHCCCCHHEEEECC
LMGDKSDNIPGVPGVGEKTAFKLIKEYGSVEAVLENIDNISGKKLKENLTEYSEQAIFSK
CCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KLATIMTNVPIDFDLDEIKSKDDYNKDQLKALFTKLQMKSILDKLSNGEEEEPEIEVKIT
HHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
NIENNKELEDLFKNINEEVYINFTYLNKEKYSTIEIDKIFINYDEKNYLINFKELVKEEE
CCCCCCHHHHHHHCCCCEEEEEEEEECCCCCCEEEEEEEEEEECCCCEEEEHHHHHCCHH
NIKSFKEFFENEAILKNGYSIKNALTALKKLGIDFNGLAFDCELAAYLIDSSKGEYTLLS
HHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCCCCHHHH
LINNYLSKFPNVKEGEEDLVGITFMKELTLVLKKKIEEEKMERLMYDIELPLETVLSSME
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
SIGFKVNKDILDELSIKFKRELEETQKEIFELADEEFNINSPKQLGKILFEKLDLPVIKK
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEE
TKTGYSTNAEVLEKLKDKHPIIEKITYFRQLSKIYSTYVEGLKAVIDTDGSIHSTFNQTV
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCE
TTTGRLSSTEPNLQNIPIKYEMGREIRKVFIPKKEGDLLISADYSQIELRVLAHISGDEN
EECCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEEECCCCH
MIDAFNHHSDIHTKTASEVFKVPVEEVTPLMRSRAKAVNFGIVYGIGDFSLSQDLNISRK
HHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCHH
EAKEYMDIYFERYPKIKEYISKVIHEAQTKGYVLTMLNRRRYIPEANASNKIVKALGERL
HHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
AMNAPIQGTAADIIKLAMVNVYNKLKELNLNSKLILQVHDELIINVVKGELDIVKKLLKE
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCHHHHHHHHHHH
EMENTIKLSVPLDVDVNVGEDWYRLK
HHCCEEEEEEEEEEECCCCCCCEECC
>Mature Secondary Structure
MKRLLILDGNSLLNRAFYAVPLLSNSEGTHTNAIYGFTNMLFKMREDIKPDYIVCTFDKK
CCEEEEECCCHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCC
APTFRHLEYGDYKAGRKKMPPELAEQFPIVKEMLKLFSITTYELDGFEADDIIGSLAKVA
CCCEECCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHH
EGEGIEVYIMTGDKDALQLAAKDINVCITKKGVSEIEVYNYDRMVEEYGVTPTQFIDVKG
CCCCEEEEEEECCHHHHHHHHCCCEEEEEECCCCCEEEECHHHHHHHHCCCCHHEEEECC
LMGDKSDNIPGVPGVGEKTAFKLIKEYGSVEAVLENIDNISGKKLKENLTEYSEQAIFSK
CCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KLATIMTNVPIDFDLDEIKSKDDYNKDQLKALFTKLQMKSILDKLSNGEEEEPEIEVKIT
HHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
NIENNKELEDLFKNINEEVYINFTYLNKEKYSTIEIDKIFINYDEKNYLINFKELVKEEE
CCCCCCHHHHHHHCCCCEEEEEEEEECCCCCCEEEEEEEEEEECCCCEEEEHHHHHCCHH
NIKSFKEFFENEAILKNGYSIKNALTALKKLGIDFNGLAFDCELAAYLIDSSKGEYTLLS
HHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCCCCHHHH
LINNYLSKFPNVKEGEEDLVGITFMKELTLVLKKKIEEEKMERLMYDIELPLETVLSSME
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
SIGFKVNKDILDELSIKFKRELEETQKEIFELADEEFNINSPKQLGKILFEKLDLPVIKK
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEE
TKTGYSTNAEVLEKLKDKHPIIEKITYFRQLSKIYSTYVEGLKAVIDTDGSIHSTFNQTV
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCE
TTTGRLSSTEPNLQNIPIKYEMGREIRKVFIPKKEGDLLISADYSQIELRVLAHISGDEN
EECCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEEECCCCH
MIDAFNHHSDIHTKTASEVFKVPVEEVTPLMRSRAKAVNFGIVYGIGDFSLSQDLNISRK
HHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCHH
EAKEYMDIYFERYPKIKEYISKVIHEAQTKGYVLTMLNRRRYIPEANASNKIVKALGERL
HHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
AMNAPIQGTAADIIKLAMVNVYNKLKELNLNSKLILQVHDELIINVVKGELDIVKKLLKE
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCHHHHHHHHHHH
EMENTIKLSVPLDVDVNVGEDWYRLK
HHCCEEEEEEEEEEECCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]