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The map label for this gene is pbpA [H]

Identifier: 18310944

GI number: 18310944

Start: 2250521

End: 2252560

Strand: Direct

Name: pbpA [H]

Synonym: CPE1962

Alternate gene names: 18310944

Gene position: 2250521-2252560 (Clockwise)

Preceding gene: 18310942

Following gene: 18310945

Centisome position: 74.24

GC content: 32.21

Gene sequence:

>2040_bases
ATGACTGAAAGAAAAAGAGAGCATAAAGATAGAAAGCAGAATAAAAATTCACCTAAAAATCAATCGAAAGTAACAAAATT
TTTGAAATGGTTCTTTATAGGGATTCTGCTTCTAGGGATAACTGCCGTAACAGTAGTTGGAATTTACGTTCTTTCTATTA
TACGTTCATCTCCAGAGTTAGATGTTCAGGCAATTCAATCTCTAAATCAGCCATCCATTCTTTACGATGATCAGGGAAAC
TTTATGGATAATGTTATAACTCGTGAACAACGTTATGTAGTTAAATCTGAAGAGATACCTGATAACTTAAAAAAGGCTTT
TGTAGCTATTGAAGACGAAAGATTTTATGAGCATAAAGGAATAGACATTAAAAGAATTTTTGGGGTAATAGCTTCTAATA
TTAAAGGTAAACTTTCAGGAAGTAATACAGTTCAAGGGGCTTCAACCATAACTCAGCAACTTATAAAAAATGCCGTACTT
ACTAATGAAGTTAGTTATGAAAGAAAAATTAAAGAAATGTACTTAGCTTTGGAATTAGAAAAGCACCTTTCAAAAGATGA
AATCCTTACTACGTATTTAAATACAATTCCTATGGGTGGATACCAATATGGGGTTAGCGCAGCTGCTCAAAGATTTTTTA
GTAAGAATGTTTCAGATTTGAATTTAGTTGAGTGCGCTTATTTAGGAGGACTTACTCAAGCACCAACTTCTTATGATGGT
CTTTCAGAAGCAAATAAAGAAAATCCAAGTAGATATTTAAATAGAACTAAATCTGTACTATTTAAAATGCATGAACTTGG
ATATATTTCAAGTGAACAATATAATGACGCAATAAATGAAATTGACACAAATGGTATAAAATTCACACCAAATAATAAAT
TAAGTAAAACTAACTTTGAGTGGTTCACAAGACCAGCTATAACTCAAGTTAAACAAGACTTAATGGATAAATATAAATAT
ACACAAGAGGAAGTTGACAAACTTATAGCTAATGGTGGATTAAAAATCTATACTTCAATGGATAGAAATCTTCAAAATAA
TGTTCAAAAAGTTTTAGATGATCCAAATAACTATAAAGCTATAACTAATAATCCTAATGAAAAAAATGAAGATGGTGTTT
ATAAATTACAAGCATCTGCCACAATAATAGACTATAAAACAGGCCATGTTAAGGCTTTAGTTGGAGGAAGAGGGGAACAA
CCTGCTATGTCTCACAATAGAGCTTATTATGATTTAAAATCTATAGGTTCTGCAACAAAACCATTAACAGTTTATGGTCC
TGCTATTGATTTAGGACTTGGTGGCGCTGGCTCTGTAGTAAATGATTCTCCATTAAGTAATAAAGAGTTATCTTCTACAG
GATATAAAGATCAACCTAAGAATGAATACAATAGTTATAGAGGCCCTTTAACTTTTAGAGAAGCAATTAAAATCTCTAGT
AACTTAGCAGCCATAAAAGTTGCTAATGAAGTAGGTGTTTCAAACTCTATAGCTTATGGAGAAAAATTAGGTCTTGTTTA
TGGACCTCATTCTAGAGGTATTTCCACAACAGCCTTAGGTCAATTCCAAAATGACCCTAATAATCCTGATGGAGGAAATA
CTTATACTCTAGCTTCAGCCTTCGGTGTTTTTGGTAATAACGGTGTTAAAACAAATGCTAAATTATATACAAAGGTATTA
GATTCTCATGGAAATGTAATTCTTGATACAAGTACTCCAGAAGAAACTAAAATATTTAGTCCTCAAGCGTCTTATATAGT
TTATGATATGCTTAAGGATCAAGTAGAAAGTGGCTCTGCAAAATCTGCTAAATTTGGTAATATTCCTGTGGCGGGTAAAA
CAGGAACTACTACTGGAGATAAAGACTATTTATTTGCAGGATTAACTCCATATTATTCTGCGGCTATTTGGATTGGATAT
GATAAGCCTAGAGAAATGAGAACTAGTAGTGGTACTGTTACCTCTCCTATTTTCGGAAAAATAATGGGCTTAGCTCATAA
AGACTTACAGTACAAAGAGGTTGACAACCTAGTGGAATAA

Upstream 100 bases:

>100_bases
TTATTATGCTATAATATAATATAATATTTCTTTAAATGTATAATAATATAATTATGTAGTTTTTTAGAAATAGAAAGTTT
TATTATGATAGGAGGAAAAA

Downstream 100 bases:

>100_bases
GCAAGGTTGCAGTATGTATGGATTCAGGTCTTAAACCTACTTCCCTTTGTACTCAAGATCCTAGAGGCAGTAGAGTTTAT
TATGATTGGTTTATTAATGG

Product: penicillin-binding proteins 1A/1B

Products: NA

Alternate protein names: PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MTERKREHKDRKQNKNSPKNQSKVTKFLKWFFIGILLLGITAVTVVGIYVLSIIRSSPELDVQAIQSLNQPSILYDDQGN
FMDNVITREQRYVVKSEEIPDNLKKAFVAIEDERFYEHKGIDIKRIFGVIASNIKGKLSGSNTVQGASTITQQLIKNAVL
TNEVSYERKIKEMYLALELEKHLSKDEILTTYLNTIPMGGYQYGVSAAAQRFFSKNVSDLNLVECAYLGGLTQAPTSYDG
LSEANKENPSRYLNRTKSVLFKMHELGYISSEQYNDAINEIDTNGIKFTPNNKLSKTNFEWFTRPAITQVKQDLMDKYKY
TQEEVDKLIANGGLKIYTSMDRNLQNNVQKVLDDPNNYKAITNNPNEKNEDGVYKLQASATIIDYKTGHVKALVGGRGEQ
PAMSHNRAYYDLKSIGSATKPLTVYGPAIDLGLGGAGSVVNDSPLSNKELSSTGYKDQPKNEYNSYRGPLTFREAIKISS
NLAAIKVANEVGVSNSIAYGEKLGLVYGPHSRGISTTALGQFQNDPNNPDGGNTYTLASAFGVFGNNGVKTNAKLYTKVL
DSHGNVILDTSTPEETKIFSPQASYIVYDMLKDQVESGSAKSAKFGNIPVAGKTGTTTGDKDYLFAGLTPYYSAAIWIGY
DKPREMRTSSGTVTSPIFGKIMGLAHKDLQYKEVDNLVE

Sequences:

>Translated_679_residues
MTERKREHKDRKQNKNSPKNQSKVTKFLKWFFIGILLLGITAVTVVGIYVLSIIRSSPELDVQAIQSLNQPSILYDDQGN
FMDNVITREQRYVVKSEEIPDNLKKAFVAIEDERFYEHKGIDIKRIFGVIASNIKGKLSGSNTVQGASTITQQLIKNAVL
TNEVSYERKIKEMYLALELEKHLSKDEILTTYLNTIPMGGYQYGVSAAAQRFFSKNVSDLNLVECAYLGGLTQAPTSYDG
LSEANKENPSRYLNRTKSVLFKMHELGYISSEQYNDAINEIDTNGIKFTPNNKLSKTNFEWFTRPAITQVKQDLMDKYKY
TQEEVDKLIANGGLKIYTSMDRNLQNNVQKVLDDPNNYKAITNNPNEKNEDGVYKLQASATIIDYKTGHVKALVGGRGEQ
PAMSHNRAYYDLKSIGSATKPLTVYGPAIDLGLGGAGSVVNDSPLSNKELSSTGYKDQPKNEYNSYRGPLTFREAIKISS
NLAAIKVANEVGVSNSIAYGEKLGLVYGPHSRGISTTALGQFQNDPNNPDGGNTYTLASAFGVFGNNGVKTNAKLYTKVL
DSHGNVILDTSTPEETKIFSPQASYIVYDMLKDQVESGSAKSAKFGNIPVAGKTGTTTGDKDYLFAGLTPYYSAAIWIGY
DKPREMRTSSGTVTSPIFGKIMGLAHKDLQYKEVDNLVE
>Mature_678_residues
TERKREHKDRKQNKNSPKNQSKVTKFLKWFFIGILLLGITAVTVVGIYVLSIIRSSPELDVQAIQSLNQPSILYDDQGNF
MDNVITREQRYVVKSEEIPDNLKKAFVAIEDERFYEHKGIDIKRIFGVIASNIKGKLSGSNTVQGASTITQQLIKNAVLT
NEVSYERKIKEMYLALELEKHLSKDEILTTYLNTIPMGGYQYGVSAAAQRFFSKNVSDLNLVECAYLGGLTQAPTSYDGL
SEANKENPSRYLNRTKSVLFKMHELGYISSEQYNDAINEIDTNGIKFTPNNKLSKTNFEWFTRPAITQVKQDLMDKYKYT
QEEVDKLIANGGLKIYTSMDRNLQNNVQKVLDDPNNYKAITNNPNEKNEDGVYKLQASATIIDYKTGHVKALVGGRGEQP
AMSHNRAYYDLKSIGSATKPLTVYGPAIDLGLGGAGSVVNDSPLSNKELSSTGYKDQPKNEYNSYRGPLTFREAIKISSN
LAAIKVANEVGVSNSIAYGEKLGLVYGPHSRGISTTALGQFQNDPNNPDGGNTYTLASAFGVFGNNGVKTNAKLYTKVLD
SHGNVILDTSTPEETKIFSPQASYIVYDMLKDQVESGSAKSAKFGNIPVAGKTGTTTGDKDYLFAGLTPYYSAAIWIGYD
KPREMRTSSGTVTSPIFGKIMGLAHKDLQYKEVDNLVE

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=575, Percent_Identity=27.6521739130435, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI87082258, Length=417, Percent_Identity=31.1750599520384, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1788867, Length=560, Percent_Identity=25.8928571428571, Blast_Score=136, Evalue=6e-33,
Organism=Escherichia coli, GI1789601, Length=183, Percent_Identity=36.6120218579235, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 75178; Mature: 75046

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERKREHKDRKQNKNSPKNQSKVTKFLKWFFIGILLLGITAVTVVGIYVLSIIRSSPEL
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DVQAIQSLNQPSILYDDQGNFMDNVITREQRYVVKSEEIPDNLKKAFVAIEDERFYEHKG
CHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHEEEECHHHHHHCC
IDIKRIFGVIASNIKGKLSGSNTVQGASTITQQLIKNAVLTNEVSYERKIKEMYLALELE
CHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
KHLSKDEILTTYLNTIPMGGYQYGVSAAAQRFFSKNVSDLNLVECAYLGGLTQAPTSYDG
HHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCC
LSEANKENPSRYLNRTKSVLFKMHELGYISSEQYNDAINEIDTNGIKFTPNNKLSKTNFE
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCH
WFTRPAITQVKQDLMDKYKYTQEEVDKLIANGGLKIYTSMDRNLQNNVQKVLDDPNNYKA
HHCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCEE
ITNNPNEKNEDGVYKLQASATIIDYKTGHVKALVGGRGEQPAMSHNRAYYDLKSIGSATK
EECCCCCCCCCCEEEEEECEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEHHHCCCCCC
PLTVYGPAIDLGLGGAGSVVNDSPLSNKELSSTGYKDQPKNEYNSYRGPLTFREAIKISS
CEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHCC
NLAAIKVANEVGVSNSIAYGEKLGLVYGPHSRGISTTALGQFQNDPNNPDGGNTYTLASA
CEEEEEEHHHHCCCCCCCCHHHCCEEECCCCCCCCHHHHHCCCCCCCCCCCCCEEEHHHH
FGVFGNNGVKTNAKLYTKVLDSHGNVILDTSTPEETKIFSPQASYIVYDMLKDQVESGSA
HHHHCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHHHCCCC
KSAKFGNIPVAGKTGTTTGDKDYLFAGLTPYYSAAIWIGYDKPREMRTSSGTVTSPIFGK
CCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHCEEEEEECCCCHHHCCCCCCCCHHHHHH
IMGLAHKDLQYKEVDNLVE
HHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
TERKREHKDRKQNKNSPKNQSKVTKFLKWFFIGILLLGITAVTVVGIYVLSIIRSSPEL
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DVQAIQSLNQPSILYDDQGNFMDNVITREQRYVVKSEEIPDNLKKAFVAIEDERFYEHKG
CHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHEEEECHHHHHHCC
IDIKRIFGVIASNIKGKLSGSNTVQGASTITQQLIKNAVLTNEVSYERKIKEMYLALELE
CHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
KHLSKDEILTTYLNTIPMGGYQYGVSAAAQRFFSKNVSDLNLVECAYLGGLTQAPTSYDG
HHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCC
LSEANKENPSRYLNRTKSVLFKMHELGYISSEQYNDAINEIDTNGIKFTPNNKLSKTNFE
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCH
WFTRPAITQVKQDLMDKYKYTQEEVDKLIANGGLKIYTSMDRNLQNNVQKVLDDPNNYKA
HHCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCEE
ITNNPNEKNEDGVYKLQASATIIDYKTGHVKALVGGRGEQPAMSHNRAYYDLKSIGSATK
EECCCCCCCCCCEEEEEECEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEHHHCCCCCC
PLTVYGPAIDLGLGGAGSVVNDSPLSNKELSSTGYKDQPKNEYNSYRGPLTFREAIKISS
CEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHCC
NLAAIKVANEVGVSNSIAYGEKLGLVYGPHSRGISTTALGQFQNDPNNPDGGNTYTLASA
CEEEEEEHHHHCCCCCCCCHHHCCEEECCCCCCCCHHHHHCCCCCCCCCCCCCEEEHHHH
FGVFGNNGVKTNAKLYTKVLDSHGNVILDTSTPEETKIFSPQASYIVYDMLKDQVESGSA
HHHHCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHHHCCCC
KSAKFGNIPVAGKTGTTTGDKDYLFAGLTPYYSAAIWIGYDKPREMRTSSGTVTSPIFGK
CCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHCEEEEEECCCCHHHCCCCCCCCHHHHHH
IMGLAHKDLQYKEVDNLVE
HHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA