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The map label for this gene is hprT [H]

Identifier: 18310942

GI number: 18310942

Start: 2249137

End: 2249661

Strand: Direct

Name: hprT [H]

Synonym: CPE1960

Alternate gene names: 18310942

Gene position: 2249137-2249661 (Clockwise)

Preceding gene: 18310913

Following gene: 18310944

Centisome position: 74.19

GC content: 29.14

Gene sequence:

>525_bases
ATGGAAGATAAAAACAGAAATATACTACTTTCTGAAGAGCAAATTAAAGAACAAATAGCTATTGTAGGTAAAAAATTAAC
TGAAGATTATAAGGATAAAAACCTTTATGTTTTATCTTTATTAAGAGGAAGTTTCATATTTGCAGCGGATTTAGTTAGAG
CAATAGATACTAAAGCTCAAATAGGATTTATGACTACTTCAAGTTATGGCCATGGTGAAGAAAGTACTGGTATAGTCAAA
GTTGTAAATGATATTCCTGATAATATTGAAGGCTTAGATGTCTTAATCGTTGATGATATCATAGATTCAGGTATAACAAT
GGATTTTGTTATAAACCATGTTAAATCACTAGGAGCTAAATCAGTTAAAACTTGCACTCTTTTAGACAAACCTTCTAGAA
GAAAAGTTGATTTAAAACCAGACTATTGTTGTTTTGAAATAGAGGACTTATTCGTTGTTGGTTACGGATTAAACTATGGT
GATCATTATAGAAATATCCCTTACATATTTAACTGGGAAAAATAA

Upstream 100 bases:

>100_bases
CCACCTCGTGATATTTATTATGTTTCTTGACAATTTTCATTTAATTTTATACTATAAATACAATATTGTAAATAATACGG
AATAGTTAGGGGAGAAAATA

Downstream 100 bases:

>100_bases
TTAAAAGGAGGAATTTCCTCCTTTTTTAATGGGCTAATTTGTATCTTCTGAATCTTCTTCATTAATACCTTGTAGCATAT
TTCCACCCCACATGCTATTT

Product: hypoxanthine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MEDKNRNILLSEEQIKEQIAIVGKKLTEDYKDKNLYVLSLLRGSFIFAADLVRAIDTKAQIGFMTTSSYGHGEESTGIVK
VVNDIPDNIEGLDVLIVDDIIDSGITMDFVINHVKSLGAKSVKTCTLLDKPSRRKVDLKPDYCCFEIEDLFVVGYGLNYG
DHYRNIPYIFNWEK

Sequences:

>Translated_174_residues
MEDKNRNILLSEEQIKEQIAIVGKKLTEDYKDKNLYVLSLLRGSFIFAADLVRAIDTKAQIGFMTTSSYGHGEESTGIVK
VVNDIPDNIEGLDVLIVDDIIDSGITMDFVINHVKSLGAKSVKTCTLLDKPSRRKVDLKPDYCCFEIEDLFVVGYGLNYG
DHYRNIPYIFNWEK
>Mature_174_residues
MEDKNRNILLSEEQIKEQIAIVGKKLTEDYKDKNLYVLSLLRGSFIFAADLVRAIDTKAQIGFMTTSSYGHGEESTGIVK
VVNDIPDNIEGLDVLIVDDIIDSGITMDFVINHVKSLGAKSVKTCTLLDKPSRRKVDLKPDYCCFEIEDLFVVGYGLNYG
DHYRNIPYIFNWEK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=178, Percent_Identity=32.0224719101124, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI9910262, Length=172, Percent_Identity=29.0697674418605, Blast_Score=84, Evalue=7e-17,
Organism=Escherichia coli, GI87081689, Length=165, Percent_Identity=46.6666666666667, Blast_Score=153, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17510817, Length=172, Percent_Identity=29.6511627906977, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 19717; Mature: 19717

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDKNRNILLSEEQIKEQIAIVGKKLTEDYKDKNLYVLSLLRGSFIFAADLVRAIDTKAQ
CCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHHHCCCCE
IGFMTTSSYGHGEESTGIVKVVNDIPDNIEGLDVLIVDDIIDSGITMDFVINHVKSLGAK
EEEEEECCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECHHCCCCHHHHHHHHHHHCCCC
SVKTCTLLDKPSRRKVDLKPDYCCFEIEDLFVVGYGLNYGDHYRNIPYIFNWEK
CCEEEEEECCCCCCEECCCCCEEEEEEEEEEEEEECCCCCCCCCCCCEEEECCC
>Mature Secondary Structure
MEDKNRNILLSEEQIKEQIAIVGKKLTEDYKDKNLYVLSLLRGSFIFAADLVRAIDTKAQ
CCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHHHCCCCE
IGFMTTSSYGHGEESTGIVKVVNDIPDNIEGLDVLIVDDIIDSGITMDFVINHVKSLGAK
EEEEEECCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECHHCCCCHHHHHHHHHHHCCCC
SVKTCTLLDKPSRRKVDLKPDYCCFEIEDLFVVGYGLNYGDHYRNIPYIFNWEK
CCEEEEEECCCCCCEECCCCCEEEEEEEEEEEEEECCCCCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]