Click here to switch to the map view.

The map label for this gene is queA

Identifier: 18310928

GI number: 18310928

Start: 2229044

End: 2230069

Strand: Reverse

Name: queA

Synonym: CPE1946

Alternate gene names: 18310928

Gene position: 2230069-2229044 (Counterclockwise)

Preceding gene: 18310929

Following gene: 18310927

Centisome position: 73.56

GC content: 29.82

Gene sequence:

>1026_bases
ATGAAAGTAAGTGATTTTTATTTTGAGCTACCAGAAGAACTTATAGCTCAATATCCACTTGAAAAGAGAGATTCTTCAAG
ATTAATGGTTTTAGATAAAAAAACTGGAGAGATTGAACATAGAAAATTCCACGATATATTAGAGTATTTAAATGAAGGAG
ATACTTTAGTTTTAAATAATACTAGAGTATTACCTGCTAGACTTATAGGAGAAAAAGAAGAAACTGGTGGTAAAATAGAA
TTTCTTCTTTTAAAGAGAATAGAAGGAGATAAGTGGGAGTGTTTAGCTAAGCCAGGAAGAAAAGCTAAAGTAGGTACAGT
TTTCACTTTTGGAGAAGGTAAACTTAAAGCCATAGTTAGAGAAATTGGTGAAGAAGGAAATAGAATAATCGAGTTTAAGT
ATGATGGTATATTTGAACAAGTTCTAGATGAATTAGGACAAATGCCACTTCCACCTTATATACATGAAAAATTAGAAGAC
AAAGAAAGATATCAAACAGTTTATTCTAAAGAAAAGGGATCAGCAGCAGCACCAACAGCTGGATTACATTTTACTGAGGA
ATTATTAAAAGAAATTAAAGATAAAGGTGTTAATATAGCATATTTAACTTTACATGTAGGACTTGGTACATTTAGACCAG
TAAAGGTTGATGATGTAAATAATCATGTTATGCATTCAGAATATTATCATTTAGATAAAGAAAATGCAGAGCTTATAAAT
AAAACTAAGGAAGCTGGAAAGAGAGTAATTGCTGTAGGAACAACATCTTCAAGAACTTTAGAAACTATTGGAGATGAAAA
TGGAAGAGTTAGAGAACAAAGTGGATGGACAGATATATTCATATATCCAGGATATAAGTTTAAAATAGTTGATAATTTAA
TTACAAACTTCCATTTACCTGAATCAACTTTAATAATGCTTGTTTCAGCTTTAGCTGGCCAAGATAACATAATGAATGCT
TATAACACTGCAGTAAAAGAAAAGTATAGATTCTTCTCATTTGGAGATTCAATGTTTATAAAATAA

Upstream 100 bases:

>100_bases
AGCATATAAACACTTAGGAAGAGTTAGATTTAATGAATCTAAGATAGATTCTAAACAGTGTACGTTATTTGAAAAATAAA
ATAGAGAAGGTGTAATTAAA

Downstream 100 bases:

>100_bases
AAAGCAAAGGAAGGACTTTTTTCATGACTAAAAAAAGATATACTCTTTTAAAAAAAGACGGAAAAGCTAGAAGAGGTGAG
TTTGTAACTCCTCACGGTAC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKVSDFYFELPEELIAQYPLEKRDSSRLMVLDKKTGEIEHRKFHDILEYLNEGDTLVLNNTRVLPARLIGEKEETGGKIE
FLLLKRIEGDKWECLAKPGRKAKVGTVFTFGEGKLKAIVREIGEEGNRIIEFKYDGIFEQVLDELGQMPLPPYIHEKLED
KERYQTVYSKEKGSAAAPTAGLHFTEELLKEIKDKGVNIAYLTLHVGLGTFRPVKVDDVNNHVMHSEYYHLDKENAELIN
KTKEAGKRVIAVGTTSSRTLETIGDENGRVREQSGWTDIFIYPGYKFKIVDNLITNFHLPESTLIMLVSALAGQDNIMNA
YNTAVKEKYRFFSFGDSMFIK

Sequences:

>Translated_341_residues
MKVSDFYFELPEELIAQYPLEKRDSSRLMVLDKKTGEIEHRKFHDILEYLNEGDTLVLNNTRVLPARLIGEKEETGGKIE
FLLLKRIEGDKWECLAKPGRKAKVGTVFTFGEGKLKAIVREIGEEGNRIIEFKYDGIFEQVLDELGQMPLPPYIHEKLED
KERYQTVYSKEKGSAAAPTAGLHFTEELLKEIKDKGVNIAYLTLHVGLGTFRPVKVDDVNNHVMHSEYYHLDKENAELIN
KTKEAGKRVIAVGTTSSRTLETIGDENGRVREQSGWTDIFIYPGYKFKIVDNLITNFHLPESTLIMLVSALAGQDNIMNA
YNTAVKEKYRFFSFGDSMFIK
>Mature_341_residues
MKVSDFYFELPEELIAQYPLEKRDSSRLMVLDKKTGEIEHRKFHDILEYLNEGDTLVLNNTRVLPARLIGEKEETGGKIE
FLLLKRIEGDKWECLAKPGRKAKVGTVFTFGEGKLKAIVREIGEEGNRIIEFKYDGIFEQVLDELGQMPLPPYIHEKLED
KERYQTVYSKEKGSAAAPTAGLHFTEELLKEIKDKGVNIAYLTLHVGLGTFRPVKVDDVNNHVMHSEYYHLDKENAELIN
KTKEAGKRVIAVGTTSSRTLETIGDENGRVREQSGWTDIFIYPGYKFKIVDNLITNFHLPESTLIMLVSALAGQDNIMNA
YNTAVKEKYRFFSFGDSMFIK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=354, Percent_Identity=48.3050847457627, Blast_Score=332, Evalue=3e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_CLOP1 (Q0TP14)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_696626.1
- ProteinModelPortal:   Q0TP14
- SMR:   Q0TP14
- STRING:   Q0TP14
- GeneID:   4203527
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_2202
- TIGR:   CPF_2202
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- ProtClustDB:   PRK00147
- BioCyc:   CPER195103:CPF_2202-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38979; Mature: 38979

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSDFYFELPEELIAQYPLEKRDSSRLMVLDKKTGEIEHRKFHDILEYLNEGDTLVLNN
CCCCHHHHHCHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEC
TRVLPARLIGEKEETGGKIEFLLLKRIEGDKWECLAKPGRKAKVGTVFTFGEGKLKAIVR
CEECHHHHCCCCCCCCCEEEEEEEEECCCCCEEEECCCCCCCEEEEEEEECCCHHHHHHH
EIGEEGNRIIEFKYDGIFEQVLDELGQMPLPPYIHEKLEDKERYQTVYSKEKGSAAAPTA
HHCCCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GLHFTEELLKEIKDKGVNIAYLTLHVGLGTFRPVKVDDVNNHVMHSEYYHLDKENAELIN
CHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCCCCEECCHHHCCCCCCHHHHH
KTKEAGKRVIAVGTTSSRTLETIGDENGRVREQSGWTDIFIYPGYKFKIVDNLITNFHLP
HHHHCCCEEEEEECCCCCHHHHHCCCCCEEEECCCCEEEEEECCEEEEEHHHHHHHCCCC
ESTLIMLVSALAGQDNIMNAYNTAVKEKYRFFSFGDSMFIK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCEECC
>Mature Secondary Structure
MKVSDFYFELPEELIAQYPLEKRDSSRLMVLDKKTGEIEHRKFHDILEYLNEGDTLVLNN
CCCCHHHHHCHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEC
TRVLPARLIGEKEETGGKIEFLLLKRIEGDKWECLAKPGRKAKVGTVFTFGEGKLKAIVR
CEECHHHHCCCCCCCCCEEEEEEEEECCCCCEEEECCCCCCCEEEEEEEECCCHHHHHHH
EIGEEGNRIIEFKYDGIFEQVLDELGQMPLPPYIHEKLEDKERYQTVYSKEKGSAAAPTA
HHCCCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GLHFTEELLKEIKDKGVNIAYLTLHVGLGTFRPVKVDDVNNHVMHSEYYHLDKENAELIN
CHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCCCCEECCHHHCCCCCCHHHHH
KTKEAGKRVIAVGTTSSRTLETIGDENGRVREQSGWTDIFIYPGYKFKIVDNLITNFHLP
HHHHCCCEEEEEECCCCCHHHHHCCCCCEEEECCCCEEEEEECCEEEEEHHHHHHHCCCC
ESTLIMLVSALAGQDNIMNAYNTAVKEKYRFFSFGDSMFIK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA