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The map label for this gene is tgt [H]

Identifier: 18310927

GI number: 18310927

Start: 2227878

End: 2229020

Strand: Reverse

Name: tgt [H]

Synonym: CPE1945

Alternate gene names: 18310927

Gene position: 2229020-2227878 (Counterclockwise)

Preceding gene: 18310928

Following gene: 18310926

Centisome position: 73.53

GC content: 32.72

Gene sequence:

>1143_bases
ATGACTAAAAAAAGATATACTCTTTTAAAAAAAGACGGAAAAGCTAGAAGAGGTGAGTTTGTAACTCCTCACGGTACAAT
TCAAACTCCTATTTTTATGAATGTAGGAACTTTAGCAGCCATAAAAGGTGCTGTTTCTTCAATGGATTTAAAAGAAATAG
GATGTCAAGTAGAACTTTCTAATACATACCATTTACATTTAAGACCAGGAGATAAGATTGTAAAGCAAATGGGAGGTTTA
CATAACTTTATGAATTGGGATAGACCAATCTTAACAGATTCAGGTGGATTCCAAGTTTTCTCATTAGCAGGAATGAGAAA
GATAAAAGAAGAGGGAGTTTATTTTAACTCACACATAGATGGTAGAAAAATATTCATGGGACCAGAAGAAAGTATGCAAA
TACAAAGTAACTTAGGTTCAACAATAGCTATGGCTTTTGATGAATGTATTCCAAACCCATCAACTAGAGAATATGTAGAA
AAGTCAGTTGCAAGAACAACAAGATGGCTTGAAAGATGTAAAAAGGAAATGGATAGATTAAATTCATTAGATGACACTGT
TAATAAAGAGCAAATGCTTTTTGGTATTAACCAAGGTGGAGTTTATGAAGATATAAGAATAGAACATGCTAAAACTATAA
GAGAAATGGATTTAGATGGATATGCTATTGGAGGATTAGCAGTTGGAGAAACTCATGAAGAAATGTATAGAGTTATAGAT
GCTGTAGTTCCTCACTTACCAGAGGATAAACCAATATATTTAATGGGGGTTGGTCTTCCATCAAATATATTAGAGGCAGT
AGAAAGAGGAGTAGACTTCTTTGATTGTGTTTTACCTGCTAGAAATGGAAGACATGGTCATGTTTTCACTAAAGAAGGTA
AAATAAACTTAATGAATGCTAAGTTTGAATTAGATGCTAGACCAATAGATGAAGGATGTCAATGTCCTGCATGTAAAAAT
TACACAAGAGCATATATAAGACACTTATTTAAGGCTAAAGAAATGTTAGCTATGAGATTATGTGTTCTTCACAATCTATA
CTTCTATAATAAGCTTATGGAGGATATAAGAGATGCTATAGATGGCGGATACTTTGCAGAATTCAAAGCTAAAAAGTTAG
AAGAATGGAATGGAAGAGCTTAA

Upstream 100 bases:

>100_bases
TAATGAATGCTTATAACACTGCAGTAAAAGAAAAGTATAGATTCTTCTCATTTGGAGATTCAATGTTTATAAAATAAAAA
GCAAAGGAAGGACTTTTTTC

Downstream 100 bases:

>100_bases
TAATATAAATTTTACGTAATAAGAAGGGAGTGGCTAGTATGAATTTTCAACAAATAGCAACAATGGTATTACCTTTTATT
TTAATGTTTGGTGTATTTTA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTKKRYTLLKKDGKARRGEFVTPHGTIQTPIFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYHLHLRPGDKIVKQMGGL
HNFMNWDRPILTDSGGFQVFSLAGMRKIKEEGVYFNSHIDGRKIFMGPEESMQIQSNLGSTIAMAFDECIPNPSTREYVE
KSVARTTRWLERCKKEMDRLNSLDDTVNKEQMLFGINQGGVYEDIRIEHAKTIREMDLDGYAIGGLAVGETHEEMYRVID
AVVPHLPEDKPIYLMGVGLPSNILEAVERGVDFFDCVLPARNGRHGHVFTKEGKINLMNAKFELDARPIDEGCQCPACKN
YTRAYIRHLFKAKEMLAMRLCVLHNLYFYNKLMEDIRDAIDGGYFAEFKAKKLEEWNGRA

Sequences:

>Translated_380_residues
MTKKRYTLLKKDGKARRGEFVTPHGTIQTPIFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYHLHLRPGDKIVKQMGGL
HNFMNWDRPILTDSGGFQVFSLAGMRKIKEEGVYFNSHIDGRKIFMGPEESMQIQSNLGSTIAMAFDECIPNPSTREYVE
KSVARTTRWLERCKKEMDRLNSLDDTVNKEQMLFGINQGGVYEDIRIEHAKTIREMDLDGYAIGGLAVGETHEEMYRVID
AVVPHLPEDKPIYLMGVGLPSNILEAVERGVDFFDCVLPARNGRHGHVFTKEGKINLMNAKFELDARPIDEGCQCPACKN
YTRAYIRHLFKAKEMLAMRLCVLHNLYFYNKLMEDIRDAIDGGYFAEFKAKKLEEWNGRA
>Mature_379_residues
TKKRYTLLKKDGKARRGEFVTPHGTIQTPIFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYHLHLRPGDKIVKQMGGLH
NFMNWDRPILTDSGGFQVFSLAGMRKIKEEGVYFNSHIDGRKIFMGPEESMQIQSNLGSTIAMAFDECIPNPSTREYVEK
SVARTTRWLERCKKEMDRLNSLDDTVNKEQMLFGINQGGVYEDIRIEHAKTIREMDLDGYAIGGLAVGETHEEMYRVIDA
VVPHLPEDKPIYLMGVGLPSNILEAVERGVDFFDCVLPARNGRHGHVFTKEGKINLMNAKFELDARPIDEGCQCPACKNY
TRAYIRHLFKAKEMLAMRLCVLHNLYFYNKLMEDIRDAIDGGYFAEFKAKKLEEWNGRA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=40.2816901408451, Blast_Score=295, Evalue=4e-80,
Organism=Homo sapiens, GI13375872, Length=253, Percent_Identity=28.0632411067194, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI1786607, Length=364, Percent_Identity=47.2527472527472, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17544680, Length=375, Percent_Identity=41.0666666666667, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI25146502, Length=212, Percent_Identity=25.9433962264151, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19920510, Length=358, Percent_Identity=40.2234636871508, Blast_Score=290, Evalue=9e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43200; Mature: 43069

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKRYTLLKKDGKARRGEFVTPHGTIQTPIFMNVGTLAAIKGAVSSMDLKEIGCQVELS
CCCCHHHHHHCCCCCCCCCEECCCCCCCCCEEECCHHHHHHHHHHHCCCHHHCCCEEEEC
NTYHLHLRPGDKIVKQMGGLHNFMNWDRPILTDSGGFQVFSLAGMRKIKEEGVYFNSHID
CEEEEEECCHHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHCCCEEECCCC
GRKIFMGPEESMQIQSNLGSTIAMAFDECIPNPSTREYVEKSVARTTRWLERCKKEMDRL
CCEEEECCCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NSLDDTVNKEQMLFGINQGGVYEDIRIEHAKTIREMDLDGYAIGGLAVGETHEEMYRVID
HHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEECCEEECCHHHHHHHHHH
AVVPHLPEDKPIYLMGVGLPSNILEAVERGVDFFDCVLPARNGRHGHVFTKEGKINLMNA
HHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEECCEEEEEEE
KFELDARPIDEGCQCPACKNYTRAYIRHLFKAKEMLAMRLCVLHNLYFYNKLMEDIRDAI
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGGYFAEFKAKKLEEWNGRA
CCCEEEHHHHHHHHHCCCCC
>Mature Secondary Structure 
TKKRYTLLKKDGKARRGEFVTPHGTIQTPIFMNVGTLAAIKGAVSSMDLKEIGCQVELS
CCCHHHHHHCCCCCCCCCEECCCCCCCCCEEECCHHHHHHHHHHHCCCHHHCCCEEEEC
NTYHLHLRPGDKIVKQMGGLHNFMNWDRPILTDSGGFQVFSLAGMRKIKEEGVYFNSHID
CEEEEEECCHHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHCCCEEECCCC
GRKIFMGPEESMQIQSNLGSTIAMAFDECIPNPSTREYVEKSVARTTRWLERCKKEMDRL
CCEEEECCCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NSLDDTVNKEQMLFGINQGGVYEDIRIEHAKTIREMDLDGYAIGGLAVGETHEEMYRVID
HHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEECCEEECCHHHHHHHHHH
AVVPHLPEDKPIYLMGVGLPSNILEAVERGVDFFDCVLPARNGRHGHVFTKEGKINLMNA
HHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEECCEEEEEEE
KFELDARPIDEGCQCPACKNYTRAYIRHLFKAKEMLAMRLCVLHNLYFYNKLMEDIRDAI
EEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGGYFAEFKAKKLEEWNGRA
CCCEEEHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA