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The map label for this gene is spo0A [H]

Identifier: 18310795

GI number: 18310795

Start: 2091331

End: 2091783

Strand: Reverse

Name: spo0A [H]

Synonym: CPE1813

Alternate gene names: 18310795

Gene position: 2091783-2091331 (Counterclockwise)

Preceding gene: 18310796

Following gene: 18310794

Centisome position: 69.0

GC content: 29.36

Gene sequence:

>453_bases
ATGAAGGAATCAAAAATATCTGTATTAATTGCTGATGATAACAAAGAGTTTTGCAATATTTTAAATGACTATTTATTAAG
TCAAAGGGATATTGTTGTTACAGGAATTGCAAAGGATGGATTGGAAGCTTTAAAACTTATAGAAGAAAAACAACCAGATT
TAGTTGTATTAGATATAATTATGCCACATTTAGATGGATTAGGTGTACTTGAAAGAATAAATTCTCTAGGATTAGAAAAA
ACTCCAAGAGTAATAGTATTATCTGCTGTTGGACAAGACAAAATTACTCAAAGAGCTATAACTTTAGGGGCAGATTATTA
TGTTGTTAAACCATTTGACATGGATGTATTTACAGAGAGAATAAGACAAATGTTTAATAGCACAATATCAGAGGAACCTA
CAAATAGTAAACCTACAATTAGTGTAGTTGAAGGTTCAAAAAGTGAGGATTAA

Upstream 100 bases:

>100_bases
TATTTTTTTTAGAAGGAAATAAATAAGTTTTGTCGAATATAGTTAAAGTAGAATAATTGTAAAAAAATGGATATATAAAG
TTGGTTAAGGAGAGAAAAGC

Downstream 100 bases:

>100_bases
TCTTCTGATGATAACAAAGAGCCTATGGATTTAGAGGTTGAAATAACTAGTATAATTCATGAAATAGGAGTTCCAGCTCA
CATAAAAGGATATATGTATT

Product: phosphorylation-activated transcription factor

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MKESKISVLIADDNKEFCNILNDYLLSQRDIVVTGIAKDGLEALKLIEEKQPDLVVLDIIMPHLDGLGVLERINSLGLEK
TPRVIVLSAVGQDKITQRAITLGADYYVVKPFDMDVFTERIRQMFNSTISEEPTNSKPTISVVEGSKSED

Sequences:

>Translated_150_residues
MKESKISVLIADDNKEFCNILNDYLLSQRDIVVTGIAKDGLEALKLIEEKQPDLVVLDIIMPHLDGLGVLERINSLGLEK
TPRVIVLSAVGQDKITQRAITLGADYYVVKPFDMDVFTERIRQMFNSTISEEPTNSKPTISVVEGSKSED
>Mature_150_residues
MKESKISVLIADDNKEFCNILNDYLLSQRDIVVTGIAKDGLEALKLIEEKQPDLVVLDIIMPHLDGLGVLERINSLGLEK
TPRVIVLSAVGQDKITQRAITLGADYYVVKPFDMDVFTERIRQMFNSTISEEPTNSKPTISVVEGSKSED

Specific function: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the ex

COG id: COG0784

COG function: function code T; FOG: CheY-like receiver

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=147, Percent_Identity=31.9727891156463, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1787487, Length=131, Percent_Identity=35.1145038167939, Blast_Score=64, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR014879
- InterPro:   IPR012052
- InterPro:   IPR011991
- ProDom:   PD010908 [H]

Pfam domain/function: PF00072 Response_reg; PF08769 Spo0A_C [H]

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 16707; Mature: 16707

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESKISVLIADDNKEFCNILNDYLLSQRDIVVTGIAKDGLEALKLIEEKQPDLVVLDII
CCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEEH
MPHLDGLGVLERINSLGLEKTPRVIVLSAVGQDKITQRAITLGADYYVVKPFDMDVFTER
HHCCCCCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHEECCCEEEEECCCHHHHHHH
IRQMFNSTISEEPTNSKPTISVVEGSKSED
HHHHHHHHHCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MKESKISVLIADDNKEFCNILNDYLLSQRDIVVTGIAKDGLEALKLIEEKQPDLVVLDII
CCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEEH
MPHLDGLGVLERINSLGLEKTPRVIVLSAVGQDKITQRAITLGADYYVVKPFDMDVFTER
HHCCCCCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHEECCCEEEEECCCHHHHHHH
IRQMFNSTISEEPTNSKPTISVVEGSKSED
HHHHHHHHHCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7885226 [H]