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The map label for this gene is spoIVB [H]

Identifier: 18310796

GI number: 18310796

Start: 2091957

End: 2093117

Strand: Reverse

Name: spoIVB [H]

Synonym: CPE1814

Alternate gene names: 18310796

Gene position: 2093117-2091957 (Counterclockwise)

Preceding gene: 18310797

Following gene: 18310795

Centisome position: 69.05

GC content: 28.77

Gene sequence:

>1161_bases
ATGAAAAATATGAACAAAAGAATAAAGATTATATCAATAATAATGATGTCTTTAATATTACTTTTATCCTCTGTAACATT
TGCAAGGGATTATTGTGAGAGTAATAATGTTTTTGCAAGTAGCAATTTCTATTCTTTAAATTCTAAGAGTAGTAATGATG
AAAAGTTTAAGAACAGATATGGCGTAGCTCTTGTAAATAGTGAATTAGAAAAAAAAGATATAGAGCTTTATGCTGGAGGA
AATTCAGTAGGTGTAAGGGTTTCAACAGATGGTGTATTAGCAGTAGGTTATTCAAGTTTAACATTGGGAGAGGGAGAAAT
AGAGAGTCCAGCGCAAAATGGTGGAATACAAATTGGTGATAGACTTATAAGTGTAAATGGAGATAAAATAAAAAATTCAA
AAGATTTATCAAAGAAAATTAATGAAAGTAAATCAGAAAATGTTGAAATACTAATTGAGAGAAATGGTGAAAAAATAACT
AAAAACATAAATTTATCAAAAAATTCAGAGGGTGATTATAAAATAGGTCTTTGGGTAAGAGATTCTACTGCTGGTGTAGG
TACACTTACTTTCTATGATAAAGAAAGTGGAAAATATGGAGCAATAGGTCATCCAATAACAGATAGTGAAACAGAAAAAA
TTCTTTCAATAAAAAATGGAGATCTTTTAAATTCTTCAATAATAAGCATAAAAAAAGGTGTTAAAGGTAATCCAGGAGAA
TTAAGAGGAATTTTTTCAAGTGATAAGAAACCAATAGGAAATGTTACAGGAAATACACAATGTGGAATCTTTGGTAGTAT
GAATACAGAAAATTTGAAAAATATTAATAATAAAACTTATAAAGTTGGTTGGAGAGATGAAATTCAGCCAGGACCAGCAC
AAATTATAACTACTATTGATGAAGAAGGTCCTAAGCTTTATGATATTGAAATTGTAAAACTTGCAAAGCAAGATAGCATT
AGTACAAAGAGTATGGTAATTAAGATTACAGATGAAAGATTATTAGAAAAAACTGGTGGTGTTGTCCAAGGAATGAGTGG
AAGTCCAATTATACAAAATGATAAAATAATTGGTGCTGTGACGCATGTTTTGGTTAATAAACCTGAAGTAGGATATGGAA
TTTATATAGAGTGGATGTTAAAAGATGCAAAAATTATATAA

Upstream 100 bases:

>100_bases
TAGTATATTTTAAATTGATTTGGCTAGAATAACAAAGTTAATACAGGATATTATTTTTTTGAAAGGGGCAAATTAAGAAT
AGAAAAGGAGGAGATAGTTA

Downstream 100 bases:

>100_bases
TAGAAAATAAGTGTGATTAATGTAAATATGTGTTATAATAAATACTAGAAAATTAAATGAATTTTTTAGTATTTATTTTT
TTTAGAAGGAAATAAATAAG

Product: stage IV sporulation protein B

Products: NA

Alternate protein names: Sporulation factor IV B protease; Stage IV sporulation protein B [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MKNMNKRIKIISIIMMSLILLLSSVTFARDYCESNNVFASSNFYSLNSKSSNDEKFKNRYGVALVNSELEKKDIELYAGG
NSVGVRVSTDGVLAVGYSSLTLGEGEIESPAQNGGIQIGDRLISVNGDKIKNSKDLSKKINESKSENVEILIERNGEKIT
KNINLSKNSEGDYKIGLWVRDSTAGVGTLTFYDKESGKYGAIGHPITDSETEKILSIKNGDLLNSSIISIKKGVKGNPGE
LRGIFSSDKKPIGNVTGNTQCGIFGSMNTENLKNINNKTYKVGWRDEIQPGPAQIITTIDEEGPKLYDIEIVKLAKQDSI
STKSMVIKITDERLLEKTGGVVQGMSGSPIIQNDKIIGAVTHVLVNKPEVGYGIYIEWMLKDAKII

Sequences:

>Translated_386_residues
MKNMNKRIKIISIIMMSLILLLSSVTFARDYCESNNVFASSNFYSLNSKSSNDEKFKNRYGVALVNSELEKKDIELYAGG
NSVGVRVSTDGVLAVGYSSLTLGEGEIESPAQNGGIQIGDRLISVNGDKIKNSKDLSKKINESKSENVEILIERNGEKIT
KNINLSKNSEGDYKIGLWVRDSTAGVGTLTFYDKESGKYGAIGHPITDSETEKILSIKNGDLLNSSIISIKKGVKGNPGE
LRGIFSSDKKPIGNVTGNTQCGIFGSMNTENLKNINNKTYKVGWRDEIQPGPAQIITTIDEEGPKLYDIEIVKLAKQDSI
STKSMVIKITDERLLEKTGGVVQGMSGSPIIQNDKIIGAVTHVLVNKPEVGYGIYIEWMLKDAKII
>Mature_386_residues
MKNMNKRIKIISIIMMSLILLLSSVTFARDYCESNNVFASSNFYSLNSKSSNDEKFKNRYGVALVNSELEKKDIELYAGG
NSVGVRVSTDGVLAVGYSSLTLGEGEIESPAQNGGIQIGDRLISVNGDKIKNSKDLSKKINESKSENVEILIERNGEKIT
KNINLSKNSEGDYKIGLWVRDSTAGVGTLTFYDKESGKYGAIGHPITDSETEKILSIKNGDLLNSSIISIKKGVKGNPGE
LRGIFSSDKKPIGNVTGNTQCGIFGSMNTENLKNINNKTYKVGWRDEIQPGPAQIITTIDEEGPKLYDIEIVKLAKQDSI
STKSMVIKITDERLLEKTGGVVQGMSGSPIIQNDKIIGAVTHVLVNKPEVGYGIYIEWMLKDAKII

Specific function: Plays a central role in the sigma-K checkpoint which coordinates gene expression during the later stages of spore formation. The protease is activated by trans cleavage of the zymogen precursor producing SpoIVB-45 kDa. This undergoes further trimming by c

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Forespore intermembrane space [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase S55 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR014219
- InterPro:   IPR008763 [H]

Pfam domain/function: PF00595 PDZ; PF05580 Peptidase_S55 [H]

EC number: =3.4.21.116 [H]

Molecular weight: Translated: 42157; Mature: 42157

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS50106 PDZ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNMNKRIKIISIIMMSLILLLSSVTFARDYCESNNVFASSNFYSLNSKSSNDEKFKNRY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCCCCHHHHHHC
GVALVNSELEKKDIELYAGGNSVGVRVSTDGVLAVGYSSLTLGEGEIESPAQNGGIQIGD
CEEEECCCCCCCCEEEEECCCEEEEEEECCCEEEECCCEEEECCCCCCCCCCCCCEEECC
RLISVNGDKIKNSKDLSKKINESKSENVEILIERNGEKITKNINLSKNSEGDYKIGLWVR
EEEEECCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCEEEEEEEE
DSTAGVGTLTFYDKESGKYGAIGHPITDSETEKILSIKNGDLLNSSIISIKKGVKGNPGE
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHEEEECCCCCCCCHHHHHHCCCCCCCCC
LRGIFSSDKKPIGNVTGNTQCGIFGSMNTENLKNINNKTYKVGWRDEIQPGPAQIITTID
EEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCEEEECCCCCCCCCCCEEEEEEC
EEGPKLYDIEIVKLAKQDSISTKSMVIKITDERLLEKTGGVVQGMSGSPIIQNDKIIGAV
CCCCEEEEEEEEEEECCCCCCCEEEEEEECHHHHHHHCCCEEECCCCCCEEECCCCHHEE
THVLVNKPEVGYGIYIEWMLKDAKII
HHHHCCCCCCCEEEEEEEEEECCCCC
>Mature Secondary Structure
MKNMNKRIKIISIIMMSLILLLSSVTFARDYCESNNVFASSNFYSLNSKSSNDEKFKNRY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCCCCHHHHHHC
GVALVNSELEKKDIELYAGGNSVGVRVSTDGVLAVGYSSLTLGEGEIESPAQNGGIQIGD
CEEEECCCCCCCCEEEEECCCEEEEEEECCCEEEECCCEEEECCCCCCCCCCCCCEEECC
RLISVNGDKIKNSKDLSKKINESKSENVEILIERNGEKITKNINLSKNSEGDYKIGLWVR
EEEEECCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCEEEEEEEE
DSTAGVGTLTFYDKESGKYGAIGHPITDSETEKILSIKNGDLLNSSIISIKKGVKGNPGE
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHEEEECCCCCCCCHHHHHHCCCCCCCCC
LRGIFSSDKKPIGNVTGNTQCGIFGSMNTENLKNINNKTYKVGWRDEIQPGPAQIITTID
EEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCEEEECCCCCCCCCCCEEEEEEC
EEGPKLYDIEIVKLAKQDSISTKSMVIKITDERLLEKTGGVVQGMSGSPIIQNDKIIGAV
CCCCEEEEEEEEEEECCCCCCCEEEEEEECHHHHHHHCCCEEECCCCCCEEECCCCHHEE
THVLVNKPEVGYGIYIEWMLKDAKII
HHHHCCCCCCCEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 11741860 [H]