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The map label for this gene is rimO

Identifier: 18310657

GI number: 18310657

Start: 1949908

End: 1951245

Strand: Reverse

Name: rimO

Synonym: CPE1675

Alternate gene names: 18310657

Gene position: 1951245-1949908 (Counterclockwise)

Preceding gene: 18310658

Following gene: 18310656

Centisome position: 64.37

GC content: 29.3

Gene sequence:

>1338_bases
TTGGCGAAATATAAGGTAGGAATGGTGAGTTTAGGTTGTGATAAAAATAGAGTTGACTCAGAAATCATGCTTGGAATGGT
TCAGAATGAGTACGAACTTACTAATAATCCAAAGGAAGCTGACATAATAATAGTAAATACTTGCGGATTTATAGAAAAGG
CTAAACAAGAATCCATAAACACAATATTAGATATGGCAAAATATAAAACAAGTCACAACTGTAAGTTACTTATAGCTACA
GGCTGCTTAACTCAAAGATATGGTGATGAATTATTAGAATTAATGCCTGAAATAGACATAATGTTAGGTGTTAATGATTA
TGCTAAAATAAATGAAGCCATAATGAACTTTATAAATGGAAATAATGAAAAAGTAAAAGCTACAAATTACAGTGATGTAT
CAATTAATGAAGGATTAAGACTTATAACAACTGATAAAGCTACTGCATACTTAAGAATTGCAGAAGGATGTGATAATTTC
TGTACTTATTGCATTATTCCTAAAATAAGAGGTAAGTTTAGAAGTAGAGCTTTAGAAAGTATTGTAGAAGAAGCGAAAAA
ACTAGCTGAAAATGGAGTGAAAGAGTTAATATTAATAGCTCAAGACACTACTAATTATGGCATAGATATATATGGTGAAA
AGAAACTTCACTTAGTTTTAAGAGAGTTAGCTAAGATCGAAGGAATAGAGTGGATAAGAGTTCTTTATTGTTATCCAGAG
GCTATATATGATGAACTTATAAAAGAGATCTCAGTAAATGATAAGGTATGTAATTATCTTGACTTACCTATACAACATAT
TAGTAATAATGTTTTAAAAAGAATGGGTAGAAAAACAACAAAAGAAGAGATAATAGGAAAAATTAATGATTTAAGAAAAA
ATGTTCCTAACATAGTTTTAAGAACTTCTTTAATAGTTGGTTTCCCAGGAGAATCTTGTGAAGATTTCAATGAACTAAAA
GATTTTATAAAGACAATAAAATTAGATAAAGTTGGAGTCTTTACTTACTCAAGAGAAGAAGGAACACCTGCTGCTATAAT
GGAAGATCAAATAGATGAAGAAGTTAAAAAAGCTAGGGAAGAAGAAATAATGTTACTTCAAAAAGAAGTATCAGAAGAAA
TTAATAAAAACAAAGTAGGTAGAGAATATGATGTTCTTATCGAAAAGTTTAATGGAGAATATTATATAGGAAGAAGCTAT
GAGATGGCTCCAGATATAGATGGATGTATTTATGTGAAAGGAAATGGAGCTAAAAAGGACCAGTTCTGTAAAGTTAAAAT
TGAAAAGGCCTTAGAATATGATTTGGTAGGAGTTGTATGCAATGAATCTTGCAAATAA

Upstream 100 bases:

>100_bases
TTAGTAAAGATGAATTAGAAAATATGAAATAAAAACTTGTTTAATTTAAAAAAGAGTTTTAAAATAAGAAATATATATTT
AAACGTAGGAGGAAGTAGAT

Downstream 100 bases:

>100_bases
ATTAACTTTAATTAGAATAATTTTAGTGCCCATATTCTTAGTATTTATAGCTTACCAAGACTTACCTTATGGGAGTGTAA
TAGCAACACTTATATTCATA

Product: hypothetical protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MAKYKVGMVSLGCDKNRVDSEIMLGMVQNEYELTNNPKEADIIIVNTCGFIEKAKQESINTILDMAKYKTSHNCKLLIAT
GCLTQRYGDELLELMPEIDIMLGVNDYAKINEAIMNFINGNNEKVKATNYSDVSINEGLRLITTDKATAYLRIAEGCDNF
CTYCIIPKIRGKFRSRALESIVEEAKKLAENGVKELILIAQDTTNYGIDIYGEKKLHLVLRELAKIEGIEWIRVLYCYPE
AIYDELIKEISVNDKVCNYLDLPIQHISNNVLKRMGRKTTKEEIIGKINDLRKNVPNIVLRTSLIVGFPGESCEDFNELK
DFIKTIKLDKVGVFTYSREEGTPAAIMEDQIDEEVKKAREEEIMLLQKEVSEEINKNKVGREYDVLIEKFNGEYYIGRSY
EMAPDIDGCIYVKGNGAKKDQFCKVKIEKALEYDLVGVVCNESCK

Sequences:

>Translated_445_residues
MAKYKVGMVSLGCDKNRVDSEIMLGMVQNEYELTNNPKEADIIIVNTCGFIEKAKQESINTILDMAKYKTSHNCKLLIAT
GCLTQRYGDELLELMPEIDIMLGVNDYAKINEAIMNFINGNNEKVKATNYSDVSINEGLRLITTDKATAYLRIAEGCDNF
CTYCIIPKIRGKFRSRALESIVEEAKKLAENGVKELILIAQDTTNYGIDIYGEKKLHLVLRELAKIEGIEWIRVLYCYPE
AIYDELIKEISVNDKVCNYLDLPIQHISNNVLKRMGRKTTKEEIIGKINDLRKNVPNIVLRTSLIVGFPGESCEDFNELK
DFIKTIKLDKVGVFTYSREEGTPAAIMEDQIDEEVKKAREEEIMLLQKEVSEEINKNKVGREYDVLIEKFNGEYYIGRSY
EMAPDIDGCIYVKGNGAKKDQFCKVKIEKALEYDLVGVVCNESCK
>Mature_444_residues
AKYKVGMVSLGCDKNRVDSEIMLGMVQNEYELTNNPKEADIIIVNTCGFIEKAKQESINTILDMAKYKTSHNCKLLIATG
CLTQRYGDELLELMPEIDIMLGVNDYAKINEAIMNFINGNNEKVKATNYSDVSINEGLRLITTDKATAYLRIAEGCDNFC
TYCIIPKIRGKFRSRALESIVEEAKKLAENGVKELILIAQDTTNYGIDIYGEKKLHLVLRELAKIEGIEWIRVLYCYPEA
IYDELIKEISVNDKVCNYLDLPIQHISNNVLKRMGRKTTKEEIIGKINDLRKNVPNIVLRTSLIVGFPGESCEDFNELKD
FIKTIKLDKVGVFTYSREEGTPAAIMEDQIDEEVKKAREEEIMLLQKEVSEEINKNKVGREYDVLIEKFNGEYYIGRSYE
MAPDIDGCIYVKGNGAKKDQFCKVKIEKALEYDLVGVVCNESCK

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=428, Percent_Identity=30.1401869158879, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI28872784, Length=428, Percent_Identity=30.1401869158879, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI93277076, Length=408, Percent_Identity=27.4509803921569, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1787057, Length=448, Percent_Identity=38.8392857142857, Blast_Score=304, Evalue=7e-84,
Organism=Escherichia coli, GI1786882, Length=392, Percent_Identity=30.8673469387755, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17553146, Length=472, Percent_Identity=26.6949152542373, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI71996771, Length=368, Percent_Identity=27.445652173913, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21356207, Length=470, Percent_Identity=28.936170212766, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI19922432, Length=453, Percent_Identity=25.3863134657837, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_CLOP1 (Q0TPS8)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_696362.2
- ProteinModelPortal:   Q0TPS8
- STRING:   Q0TPS8
- GeneID:   4202610
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_1929
- TIGR:   CPF_1929
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- ProtClustDB:   CLSK882944
- BioCyc:   CPER195103:CPF_1929-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50622; Mature: 50491

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYKVGMVSLGCDKNRVDSEIMLGMVQNEYELTNNPKEADIIIVNTCGFIEKAKQESIN
CCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
TILDMAKYKTSHNCKLLIATGCLTQRYGDELLELMPEIDIMLGVNDYAKINEAIMNFING
HHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCC
NNEKVKATNYSDVSINEGLRLITTDKATAYLRIAEGCDNFCTYCIIPKIRGKFRSRALES
CCCEEEECCCCCCCCCCCEEEEEECCCEEEEEEHHCCCCHHHEEEEHHHHHHHHHHHHHH
IVEEAKKLAENGVKELILIAQDTTNYGIDIYGEKKLHLVLRELAKIEGIEWIRVLYCYPE
HHHHHHHHHHCCHHEEEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCCEEHHEECCCH
AIYDELIKEISVNDKVCNYLDLPIQHISNNVLKRMGRKTTKEEIIGKINDLRKNVPNIVL
HHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEE
RTSLIVGFPGESCEDFNELKDFIKTIKLDKVGVFTYSREEGTPAAIMEDQIDEEVKKARE
EEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
EEIMLLQKEVSEEINKNKVGREYDVLIEKFNGEYYIGRSYEMAPDIDGCIYVKGNGAKKD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEECCCCCCC
QFCKVKIEKALEYDLVGVVCNESCK
CEEEEEHHHHHHHHHEEEECCCCCC
>Mature Secondary Structure 
AKYKVGMVSLGCDKNRVDSEIMLGMVQNEYELTNNPKEADIIIVNTCGFIEKAKQESIN
CCEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
TILDMAKYKTSHNCKLLIATGCLTQRYGDELLELMPEIDIMLGVNDYAKINEAIMNFING
HHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCC
NNEKVKATNYSDVSINEGLRLITTDKATAYLRIAEGCDNFCTYCIIPKIRGKFRSRALES
CCCEEEECCCCCCCCCCCEEEEEECCCEEEEEEHHCCCCHHHEEEEHHHHHHHHHHHHHH
IVEEAKKLAENGVKELILIAQDTTNYGIDIYGEKKLHLVLRELAKIEGIEWIRVLYCYPE
HHHHHHHHHHCCHHEEEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCCEEHHEECCCH
AIYDELIKEISVNDKVCNYLDLPIQHISNNVLKRMGRKTTKEEIIGKINDLRKNVPNIVL
HHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEE
RTSLIVGFPGESCEDFNELKDFIKTIKLDKVGVFTYSREEGTPAAIMEDQIDEEVKKARE
EEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
EEIMLLQKEVSEEINKNKVGREYDVLIEKFNGEYYIGRSYEMAPDIDGCIYVKGNGAKKD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEECCCCCCC
QFCKVKIEKALEYDLVGVVCNESCK
CEEEEEHHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA