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The map label for this gene is thiH [H]

Identifier: 18310582

GI number: 18310582

Start: 1873057

End: 1874160

Strand: Reverse

Name: thiH [H]

Synonym: CPE1600

Alternate gene names: 18310582

Gene position: 1874160-1873057 (Counterclockwise)

Preceding gene: 18310583

Following gene: 18310581

Centisome position: 61.82

GC content: 31.16

Gene sequence:

>1104_bases
TTGAGTTTTTATGATGTAGTAGAAAAATATAGAGATTTTGATTTTTATGGATATTTTGATTCTGTAAAAAAGGAAGATGT
ATTAAGAAGTATTTATGAAAGAAATAAGAGACCAGAGGATTTACTTAATTTAATATCTCCTATGGGAGAAGAGCTTTTAG
AAGAAATGGCTCAAGAAGCAAGAAATCTCTCCTTAAAATATTTTGGAAGAACAATATTACTTTATACACCTATGTATATC
TCAAATTATTGTGTAAATAAGTGTTCATATTGTGGGTATAATGTAGAAAATAAAATATGTAGGAAAAAATTAAATCAAGA
AGAAATAGAAAAAGAGGGGGAAGCTATTTCAAAGGAGGGATTTAAACATATTCTAATATTAACAGGGGAAAGTGAATATC
ATACTCCAGTAGAGTATATAGAAGAGAGCATTAAAACTTTGAAAGAGAAATTTCCTTCCATAACCATTGAAATATATCCA
ATGACAGAAGAGGGATATAAAAAGGTGGTAGAAGCAGGTGCTGAAGGGCTTACTGTATATCAAGAGACCTATGATGAAAA
GGTATATGATAGGGTTCATGTGGCTGGTCCAAAGAAAAATTATAAATTTAGATTAGAAGCTCCAGAGAGAGGAGCAGAAG
CTGGAATGAGAAGCATAAGTATAGGAGCCTTATTAGGATTAGCTGATTTTAGGATAGATGCCTTCTTTACAGCAATGCAT
GGAAAATATTTAAGAGATAAGTATCCTCATATAGATATAAGTTATTCAGTGCCAAGAATAAGACCATGTGAAGGAGGGCT
TAAAAAGTTAAATGAAGTTGATGATAGGGAACTAGTTCAAATACTTTTAGCCTATAGACTATTTGATCCTCAAGGGGGAA
TAAATATATCTACTAGAGAAGGAAAGGATTTTAGAAGAAATTTAATTCCCTTAGGAGTGAGTAAAATCAGTGCTGGAGTT
TCTACTGAAGTTGGAGGCCATTCTTTAAAAGAAAAAGGTACAAGCCAATTTGATATAAATGATGAAAGTTCTGTAAGTGA
AGTTAAGGAATTAATAAAAAGTCAAGGTTATCAACCTATATTTAAGGATTGGCATAGATTTTAA

Upstream 100 bases:

>100_bases
AGGTGGCAGATTAGCTTATGAAGCTAAAATGGGAAGAGAATCAAAGTTTGGAAATGCTTCATCTCCACTTACTGGATTTT
TAGATTAGGAGATGATTTAA

Downstream 100 bases:

>100_bases
AATAATTTTTTATTAAAATGGTGATTTTTATGTTTTTAATAACCAATAGAAAGTTAGTAAATAGGGAAAGATATTTTAAT
ACTATAGAGGAAGCAGGTAA

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSFYDVVEKYRDFDFYGYFDSVKKEDVLRSIYERNKRPEDLLNLISPMGEELLEEMAQEARNLSLKYFGRTILLYTPMYI
SNYCVNKCSYCGYNVENKICRKKLNQEEIEKEGEAISKEGFKHILILTGESEYHTPVEYIEESIKTLKEKFPSITIEIYP
MTEEGYKKVVEAGAEGLTVYQETYDEKVYDRVHVAGPKKNYKFRLEAPERGAEAGMRSISIGALLGLADFRIDAFFTAMH
GKYLRDKYPHIDISYSVPRIRPCEGGLKKLNEVDDRELVQILLAYRLFDPQGGINISTREGKDFRRNLIPLGVSKISAGV
STEVGGHSLKEKGTSQFDINDESSVSEVKELIKSQGYQPIFKDWHRF

Sequences:

>Translated_367_residues
MSFYDVVEKYRDFDFYGYFDSVKKEDVLRSIYERNKRPEDLLNLISPMGEELLEEMAQEARNLSLKYFGRTILLYTPMYI
SNYCVNKCSYCGYNVENKICRKKLNQEEIEKEGEAISKEGFKHILILTGESEYHTPVEYIEESIKTLKEKFPSITIEIYP
MTEEGYKKVVEAGAEGLTVYQETYDEKVYDRVHVAGPKKNYKFRLEAPERGAEAGMRSISIGALLGLADFRIDAFFTAMH
GKYLRDKYPHIDISYSVPRIRPCEGGLKKLNEVDDRELVQILLAYRLFDPQGGINISTREGKDFRRNLIPLGVSKISAGV
STEVGGHSLKEKGTSQFDINDESSVSEVKELIKSQGYQPIFKDWHRF
>Mature_366_residues
SFYDVVEKYRDFDFYGYFDSVKKEDVLRSIYERNKRPEDLLNLISPMGEELLEEMAQEARNLSLKYFGRTILLYTPMYIS
NYCVNKCSYCGYNVENKICRKKLNQEEIEKEGEAISKEGFKHILILTGESEYHTPVEYIEESIKTLKEKFPSITIEIYPM
TEEGYKKVVEAGAEGLTVYQETYDEKVYDRVHVAGPKKNYKFRLEAPERGAEAGMRSISIGALLGLADFRIDAFFTAMHG
KYLRDKYPHIDISYSVPRIRPCEGGLKKLNEVDDRELVQILLAYRLFDPQGGINISTREGKDFRRNLIPLGVSKISAGVS
TEVGGHSLKEKGTSQFDINDESSVSEVKELIKSQGYQPIFKDWHRF

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=340, Percent_Identity=37.9411764705882, Blast_Score=283, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42343; Mature: 42211

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFYDVVEKYRDFDFYGYFDSVKKEDVLRSIYERNKRPEDLLNLISPMGEELLEEMAQEA
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RNLSLKYFGRTILLYTPMYISNYCVNKCSYCGYNVENKICRKKLNQEEIEKEGEAISKEG
HCCCHHHHCCEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
FKHILILTGESEYHTPVEYIEESIKTLKEKFPSITIEIYPMTEEGYKKVVEAGAEGLTVY
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCCCCCCHH
QETYDEKVYDRVHVAGPKKNYKFRLEAPERGAEAGMRSISIGALLGLADFRIDAFFTAMH
HHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKYLRDKYPHIDISYSVPRIRPCEGGLKKLNEVDDRELVQILLAYRLFDPQGGINISTRE
CHHHHCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEECCCC
GKDFRRNLIPLGVSKISAGVSTEVGGHSLKEKGTSQFDINDESSVSEVKELIKSQGYQPI
CHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHCCCCEECCCCCCCHHHHHHHHHHCCCCHH
FKDWHRF
HHHHCCC
>Mature Secondary Structure 
SFYDVVEKYRDFDFYGYFDSVKKEDVLRSIYERNKRPEDLLNLISPMGEELLEEMAQEA
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RNLSLKYFGRTILLYTPMYISNYCVNKCSYCGYNVENKICRKKLNQEEIEKEGEAISKEG
HCCCHHHHCCEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
FKHILILTGESEYHTPVEYIEESIKTLKEKFPSITIEIYPMTEEGYKKVVEAGAEGLTVY
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCCCCCCHH
QETYDEKVYDRVHVAGPKKNYKFRLEAPERGAEAGMRSISIGALLGLADFRIDAFFTAMH
HHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKYLRDKYPHIDISYSVPRIRPCEGGLKKLNEVDDRELVQILLAYRLFDPQGGINISTRE
CHHHHCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEECCCC
GKDFRRNLIPLGVSKISAGVSTEVGGHSLKEKGTSQFDINDESSVSEVKELIKSQGYQPI
CHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHCCCCEECCCCCCCHHHHHHHHHHCCCCHH
FKDWHRF
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]