| Definition | Clostridium perfringens str. 13, complete genome. |
|---|---|
| Accession | NC_003366 |
| Length | 3,031,430 |
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The map label for this gene is pulA [H]
Identifier: 18310534
GI number: 18310534
Start: 1819759
End: 1821726
Strand: Reverse
Name: pulA [H]
Synonym: CPE1552
Alternate gene names: 18310534
Gene position: 1821726-1819759 (Counterclockwise)
Preceding gene: 18310536
Following gene: 18310533
Centisome position: 60.09
GC content: 29.07
Gene sequence:
>1968_bases ATGAGAAGAAATTTTAATTCTAAAGAGTTTAATGATAAGTATTATTATGATGGAGAACTTGGAGCTATTTATAATAAAGA AGAGACTATTTTTAGGGTATGGTCACCAGAAGCTAAGAGTTTAGAATTATTACTTTACAGAGATGGGAATACAGAGAGTT TAATAGAAAGAGATTTGCTTCATAAAAAAAGTAATGGACTTTGGGAAATAGTAAAAAAGGGAGATTTAAATGGAGTTTAT TATAAGTACCATATTGTAGGAGAAGATTTTGAGCATGATTTTGTTGATCCTTACTGCAAAGCCTTAGGAGTTAATGGGTA TAGAGCTATGGTTATTGACTTGAAGAAAACTAATCCTAAGGGATGGGAAAATGAAAAGAAACCATCACTTGAAAATCCCT TAGATTCAATTTTATATGAAATGCATATAAGAGATTTTTCGATTGATAAGAGTTCAGGGGTTTCTTTAGAAAATAGAGGA AAGTTTTTAGGAATAATTGAAGAAAATACAAAAGTTCCTGGTACAGAAGTAAAAACAACCTTAGATCATTTAAAGGAATT AGGAATAACCCATGTACATCTTTTACCTTCCTTTGATTTTGGAACAGTTGATGAGGAGAGATTAGATGAAGAGCAGTATA ATTGGGGATATGACCCTGTAAATTATAATGTACCAGAAGGATCATATTCTAAAAATCCATATAAAGGTGAAGTTAGAATT AAAGAGTTTAAGGAAATGGTACTTAAACTTCATAGAGCAGGAATAAGAGTTATAATGGATGTTGTGTATAATCACACTTA TTCAGGAGAAAACTCTAATTTAAATTTATCTTACCCAGGATATTATCATAGACAAGATGACTTTGGTAATTTTTCTAATG GTTCTGGATGTGGTAATGAATTAGCATCAGAAAGACTTATGGTAAGAAAATATATGGTTGATTCATTAAAGTATTGGGCA AAAGAATATCACATAGATGGATTTAGATTTGATTTAATGGCATTACATGATATTGAAACCTTAAAGGAAATAAGAGAAGA ATTAAATAAAATAGATCCTTCAATATTAATATATGGAGAAGGATGGAATGGAGGAGACTCTCCACTGCCTAAAGAAGAAG CTTGTTTTAAATGTAATATAAGTAAATTTGATAAATTACAAATAGCAGCTTTTAGTGATGATATGAGAGATGGGATAAAA GGACATGTAGCACATCTTAAAGAAGGAGGCTTCGTAAATGGAGGAGAAGACTTTGAAGAGAGTATAAAGTTTGGAATAGT AGCTTCTACTTATCATGAAGGAGTAGATTATAATAAAGTAAATTATTCAGATTCTCCTTGGGCAAATGAACCTTATCAGA CAGTAAATTATTGCTCAGCCCATGATAACAATACATTACACGATAAGCTTAAAATTGTATGTGAAAATGCTTCTGAAGAA GAGATAATAGAAATGAATAAGTTAAGTGCTGCTATTTTCTTAACATCTCAAGGAATACCCTTTATTCATTCAGGAGAGGA GTTTTTAAGAACTAAGACTAATGAAAAAGGAGAATTTATTGAAAATAGTTATAATTCTAATGACTTTGTAAACAAGATTG ATTGGACTAGAAAAGTAAAGTATATGGATTTATTTAAATACTATAAGGGATTAATTGAATTAAGAAAGGAATATCCTTTA TTTAGGTTAGAAAGTAACAAAGAAATAAGAGAAAAAATTTCATTTATAGAGAGTGAGTTAGGAATAAAGAAAAAGGGTAT AGTTGCTTATAGATTAAAAGATCATAATAATGAATTCATAGTAGTTTTTAATAGTAATAATAATGAAGTTAAAATAAACC TTCCTAAGGGTCTTTGGGGAGTTATGGTTAATAATAAATTTTCAGGAAAAGAAATAAGGGATGAAGCTAGAGATTTTTAT GATATAATAAGAAAGTCAGCATGTGTTCTTAAAAAATTAAGTGATTAA
Upstream 100 bases:
>100_bases ACTTTAAGTTGAAATTTAATTACTACTTTAATATAATTAAGTTAGTTGTAATTTATATACAGTTAGTTGGAAACGTTACC GAAATTGGGGAGAGGATTTT
Downstream 100 bases:
>100_bases ATAGAATTATAAAGTTTATAGGTTGCAATAAAATAACTTTTGATACAGATACTTGTTTAAAATAGAGCTGATTATGAAAT TTTCATATAGCTCTATTTTT
Product: pullulanase
Products: NA
Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]
Number of amino acids: Translated: 655; Mature: 655
Protein sequence:
>655_residues MRRNFNSKEFNDKYYYDGELGAIYNKEETIFRVWSPEAKSLELLLYRDGNTESLIERDLLHKKSNGLWEIVKKGDLNGVY YKYHIVGEDFEHDFVDPYCKALGVNGYRAMVIDLKKTNPKGWENEKKPSLENPLDSILYEMHIRDFSIDKSSGVSLENRG KFLGIIEENTKVPGTEVKTTLDHLKELGITHVHLLPSFDFGTVDEERLDEEQYNWGYDPVNYNVPEGSYSKNPYKGEVRI KEFKEMVLKLHRAGIRVIMDVVYNHTYSGENSNLNLSYPGYYHRQDDFGNFSNGSGCGNELASERLMVRKYMVDSLKYWA KEYHIDGFRFDLMALHDIETLKEIREELNKIDPSILIYGEGWNGGDSPLPKEEACFKCNISKFDKLQIAAFSDDMRDGIK GHVAHLKEGGFVNGGEDFEESIKFGIVASTYHEGVDYNKVNYSDSPWANEPYQTVNYCSAHDNNTLHDKLKIVCENASEE EIIEMNKLSAAIFLTSQGIPFIHSGEEFLRTKTNEKGEFIENSYNSNDFVNKIDWTRKVKYMDLFKYYKGLIELRKEYPL FRLESNKEIREKISFIESELGIKKKGIVAYRLKDHNNEFIVVFNSNNNEVKINLPKGLWGVMVNNKFSGKEIRDEARDFY DIIRKSACVLKKLSD
Sequences:
>Translated_655_residues MRRNFNSKEFNDKYYYDGELGAIYNKEETIFRVWSPEAKSLELLLYRDGNTESLIERDLLHKKSNGLWEIVKKGDLNGVY YKYHIVGEDFEHDFVDPYCKALGVNGYRAMVIDLKKTNPKGWENEKKPSLENPLDSILYEMHIRDFSIDKSSGVSLENRG KFLGIIEENTKVPGTEVKTTLDHLKELGITHVHLLPSFDFGTVDEERLDEEQYNWGYDPVNYNVPEGSYSKNPYKGEVRI KEFKEMVLKLHRAGIRVIMDVVYNHTYSGENSNLNLSYPGYYHRQDDFGNFSNGSGCGNELASERLMVRKYMVDSLKYWA KEYHIDGFRFDLMALHDIETLKEIREELNKIDPSILIYGEGWNGGDSPLPKEEACFKCNISKFDKLQIAAFSDDMRDGIK GHVAHLKEGGFVNGGEDFEESIKFGIVASTYHEGVDYNKVNYSDSPWANEPYQTVNYCSAHDNNTLHDKLKIVCENASEE EIIEMNKLSAAIFLTSQGIPFIHSGEEFLRTKTNEKGEFIENSYNSNDFVNKIDWTRKVKYMDLFKYYKGLIELRKEYPL FRLESNKEIREKISFIESELGIKKKGIVAYRLKDHNNEFIVVFNSNNNEVKINLPKGLWGVMVNNKFSGKEIRDEARDFY DIIRKSACVLKKLSD >Mature_655_residues MRRNFNSKEFNDKYYYDGELGAIYNKEETIFRVWSPEAKSLELLLYRDGNTESLIERDLLHKKSNGLWEIVKKGDLNGVY YKYHIVGEDFEHDFVDPYCKALGVNGYRAMVIDLKKTNPKGWENEKKPSLENPLDSILYEMHIRDFSIDKSSGVSLENRG KFLGIIEENTKVPGTEVKTTLDHLKELGITHVHLLPSFDFGTVDEERLDEEQYNWGYDPVNYNVPEGSYSKNPYKGEVRI KEFKEMVLKLHRAGIRVIMDVVYNHTYSGENSNLNLSYPGYYHRQDDFGNFSNGSGCGNELASERLMVRKYMVDSLKYWA KEYHIDGFRFDLMALHDIETLKEIREELNKIDPSILIYGEGWNGGDSPLPKEEACFKCNISKFDKLQIAAFSDDMRDGIK GHVAHLKEGGFVNGGEDFEESIKFGIVASTYHEGVDYNKVNYSDSPWANEPYQTVNYCSAHDNNTLHDKLKIVCENASEE EIIEMNKLSAAIFLTSQGIPFIHSGEEFLRTKTNEKGEFIENSYNSNDFVNKIDWTRKVKYMDLFKYYKGLIELRKEYPL FRLESNKEIREKISFIESELGIKKKGIVAYRLKDHNNEFIVVFNSNNNEVKINLPKGLWGVMVNNKFSGKEIRDEARDFY DIIRKSACVLKKLSD
Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]
COG id: COG1523
COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 13 family [H]
Homologues:
Organism=Escherichia coli, GI2367229, Length=569, Percent_Identity=24.6045694200351, Blast_Score=126, Evalue=4e-30,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006047 - InterPro: IPR004193 - InterPro: IPR017853 - InterPro: IPR013781 - InterPro: IPR013783 - InterPro: IPR014756 - InterPro: IPR005323 - InterPro: IPR011840 [H]
Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF03714 PUD [H]
EC number: =3.2.1.41 [H]
Molecular weight: Translated: 75949; Mature: 75949
Theoretical pI: Translated: 5.58; Mature: 5.58
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 1.8 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRRNFNSKEFNDKYYYDGELGAIYNKEETIFRVWSPEAKSLELLLYRDGNTESLIERDLL CCCCCCCCCCCCCEEECCCCCEEECCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHHH HKKSNGLWEIVKKGDLNGVYYKYHIVGEDFEHDFVDPYCKALGVNGYRAMVIDLKKTNPK HHCCCCCEEHHHCCCCCCEEEEEEEECCCCCCCHHCHHHHHHCCCCEEEEEEEEECCCCC GWENEKKPSLENPLDSILYEMHIRDFSIDKSSGVSLENRGKFLGIIEENTKVPGTEVKTT CCCCCCCCCCCCHHHHHHHHHHHCEECCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHH LDHLKELGITHVHLLPSFDFGTVDEERLDEEQYNWGYDPVNYNVPEGSYSKNPYKGEVRI HHHHHHCCCEEEEEECCCCCCCCCHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEH KEFKEMVLKLHRAGIRVIMDVVYNHTYSGENSNLNLSYPGYYHRQDDFGNFSNGSGCGNE HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHH LASERLMVRKYMVDSLKYWAKEYHIDGFRFDLMALHDIETLKEIREELNKIDPSILIYGE HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEC GWNGGDSPLPKEEACFKCNISKFDKLQIAAFSDDMRDGIKGHVAHLKEGGFVNGGEDFEE CCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCHHHHCHHHHHHHHCCCCCCCCCCCHHH SIKFGIVASTYHEGVDYNKVNYSDSPWANEPYQTVNYCSAHDNNTLHDKLKIVCENASEE HHHEEEEEEHHHCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCHHHHHHEEECCCCHH EIIEMNKLSAAIFLTSQGIPFIHSGEEFLRTKTNEKGEFIENSYNSNDFVNKIDWTRKVK HHHHHHHCEEEEEEECCCCCEEECCHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHH YMDLFKYYKGLIELRKEYPLFRLESNKEIREKISFIESELGIKKKGIVAYRLKDHNNEFI HHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEE VVFNSNNNEVKINLPKGLWGVMVNNKFSGKEIRDEARDFYDIIRKSACVLKKLSD EEEECCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure MRRNFNSKEFNDKYYYDGELGAIYNKEETIFRVWSPEAKSLELLLYRDGNTESLIERDLL CCCCCCCCCCCCCEEECCCCCEEECCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHHH HKKSNGLWEIVKKGDLNGVYYKYHIVGEDFEHDFVDPYCKALGVNGYRAMVIDLKKTNPK HHCCCCCEEHHHCCCCCCEEEEEEEECCCCCCCHHCHHHHHHCCCCEEEEEEEEECCCCC GWENEKKPSLENPLDSILYEMHIRDFSIDKSSGVSLENRGKFLGIIEENTKVPGTEVKTT CCCCCCCCCCCCHHHHHHHHHHHCEECCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHH LDHLKELGITHVHLLPSFDFGTVDEERLDEEQYNWGYDPVNYNVPEGSYSKNPYKGEVRI HHHHHHCCCEEEEEECCCCCCCCCHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEH KEFKEMVLKLHRAGIRVIMDVVYNHTYSGENSNLNLSYPGYYHRQDDFGNFSNGSGCGNE HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHH LASERLMVRKYMVDSLKYWAKEYHIDGFRFDLMALHDIETLKEIREELNKIDPSILIYGE HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEC GWNGGDSPLPKEEACFKCNISKFDKLQIAAFSDDMRDGIKGHVAHLKEGGFVNGGEDFEE CCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCHHHHCHHHHHHHHCCCCCCCCCCCHHH SIKFGIVASTYHEGVDYNKVNYSDSPWANEPYQTVNYCSAHDNNTLHDKLKIVCENASEE HHHEEEEEEHHHCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCHHHHHHEEECCCCHH EIIEMNKLSAAIFLTSQGIPFIHSGEEFLRTKTNEKGEFIENSYNSNDFVNKIDWTRKVK HHHHHHHCEEEEEEECCCCCEEECCHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHH YMDLFKYYKGLIELRKEYPLFRLESNKEIREKISFIESELGIKKKGIVAYRLKDHNNEFI HHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEE VVFNSNNNEVKINLPKGLWGVMVNNKFSGKEIRDEARDFYDIIRKSACVLKKLSD EEEECCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9453151; 10360571 [H]