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The map label for this gene is sacA [H]

Identifier: 18310514

GI number: 18310514

Start: 1793893

End: 1795356

Strand: Reverse

Name: sacA [H]

Synonym: CPE1532

Alternate gene names: 18310514

Gene position: 1795356-1793893 (Counterclockwise)

Preceding gene: 18310515

Following gene: 18310513

Centisome position: 59.22

GC content: 27.87

Gene sequence:

>1464_bases
ATGGAGTTTTTAAATGATGAAGATATAAACAAAAAATCAAAAGAATATTATAATAAAACTAAAGATATATGGAGAGATAA
TTTTCATTTTGAGCCTCCCTTTGGGCTTATAAATGATCCAAATGGATTAAGTTATTATAAAGGAGAATATTACATATTTT
TTCAGTGGAACCCATATGGCTGTGAGCACAAGAATAAACATTGGGGAATTGTAAAAACAAAGAACTTTAAAGATTTTTCA
ATACCTAAAATTGCTTTAACTCCAAAAGAATATTTTGATAAGGATGGATGTTATTCAGGGGGATCTATTGTTAAGGATGG
TAACTTACAAGTGTTTTATACTGGGAATGTTAAAGGGGAGAAAGGAGAGAGAATATCTTATCAATGCAAGGGGATTCTTG
AAGAAAATGGACAATTGAAAAAATTAGGCCCAGTTATTTCTACATTACCCAAAGGATATACAGCTCATTTTAGAGATCCA
TATATTTGGGAAAATAATGGTTATTATTATATGATTCTTGGAATACAGACTGAAAAGCTAAAGGGTAGATGCGTAATATA
TAAATCAAATGATCTAGATAATTGGAAGTTTTATGGGGAACTAAAAACGTCTTATGAGGATTTTGGATTTATGTGGGAAT
GTCCATCTTTTTTTAAAATTGATAATAAGGATATTTTAATATTTTCTCCTCAAGGAATTAAAGAAGAAGAGTTTAGATAT
CAAAACATATATCAATCGGGATACTTAATAGGAAATTTAAATTATGATACTTTAGAATTTAAAAATAATAATGAGTTCAA
GGAATTAGATATGGGCTTTGATTTTTATGCTCCTCAAGTATTTAAGGACGAAAAAGGAAGAAATATATTAATTGGATGGA
TGGGGCTTCCTGAAAAAGAAGAGTTTCATTGTAGTAGGGAGAATGGATGGATATTTTCTTTAACCATGCCTAGAGAATTA
ACCTTAAAGAATGGAGTTTTACATCAAAATCCTATAGAAGAGATGAGAAAGCTTAGGGGAGATAAAATTGTAAGCTTAAA
AAATATACTCATTGAAGATTTAAATTTTAATGAAGTAAAAGAAAATAGTTATGAGCTTAAACTAGATATAGAAAGACGGG
AAGCATCAAAGATTGAAATTAATTTTATGGAGAGTAATGAGGAACATACCTCTTTAATTTATGATTTTAAAAAAGAAATT
TGTATAATAGATAGAAATTCAATGATTAATGGAGAAAAGGGTATAAGGAAGTTTAAGTTACATTCTAATGGGAATTTAAA
AATTAATATGTTTGTGGATAAATCATCAGTAGAAATATATTTTCAAGATGGAATTGAAGTAGCATCATTAAAGCTTTACC
CTAAAAAAGATAGCTTTAATTTAAGTTTAAAATCAGAAGAGGGTAAAATTAAAATAAATTCCTTGAGTATATGGGAGATG
AATGAGGTGAATTATAATGAATAA

Upstream 100 bases:

>100_bases
AAATACTTGTGAAATTCCTTTAGAACTTATGGTAAGAGAAAGTGTTAAAAGTTTAAAGTAGGGTTTGCTTCACCGAATAA
TTTAAAGAAAGGTGAAGTAA

Downstream 100 bases:

>100_bases
AGTATTTTGTATAGGAGAGTTATTAATAGATTTTATAGGAAAAGATATAGGAAAAGGTCTTAAAAATGGAGTAAACTTTG
AGAAAAAGGCTGGTGGAGCT

Product: sucrose-6-P hydrolase

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MEFLNDEDINKKSKEYYNKTKDIWRDNFHFEPPFGLINDPNGLSYYKGEYYIFFQWNPYGCEHKNKHWGIVKTKNFKDFS
IPKIALTPKEYFDKDGCYSGGSIVKDGNLQVFYTGNVKGEKGERISYQCKGILEENGQLKKLGPVISTLPKGYTAHFRDP
YIWENNGYYYMILGIQTEKLKGRCVIYKSNDLDNWKFYGELKTSYEDFGFMWECPSFFKIDNKDILIFSPQGIKEEEFRY
QNIYQSGYLIGNLNYDTLEFKNNNEFKELDMGFDFYAPQVFKDEKGRNILIGWMGLPEKEEFHCSRENGWIFSLTMPREL
TLKNGVLHQNPIEEMRKLRGDKIVSLKNILIEDLNFNEVKENSYELKLDIERREASKIEINFMESNEEHTSLIYDFKKEI
CIIDRNSMINGEKGIRKFKLHSNGNLKINMFVDKSSVEIYFQDGIEVASLKLYPKKDSFNLSLKSEEGKIKINSLSIWEM
NEVNYNE

Sequences:

>Translated_487_residues
MEFLNDEDINKKSKEYYNKTKDIWRDNFHFEPPFGLINDPNGLSYYKGEYYIFFQWNPYGCEHKNKHWGIVKTKNFKDFS
IPKIALTPKEYFDKDGCYSGGSIVKDGNLQVFYTGNVKGEKGERISYQCKGILEENGQLKKLGPVISTLPKGYTAHFRDP
YIWENNGYYYMILGIQTEKLKGRCVIYKSNDLDNWKFYGELKTSYEDFGFMWECPSFFKIDNKDILIFSPQGIKEEEFRY
QNIYQSGYLIGNLNYDTLEFKNNNEFKELDMGFDFYAPQVFKDEKGRNILIGWMGLPEKEEFHCSRENGWIFSLTMPREL
TLKNGVLHQNPIEEMRKLRGDKIVSLKNILIEDLNFNEVKENSYELKLDIERREASKIEINFMESNEEHTSLIYDFKKEI
CIIDRNSMINGEKGIRKFKLHSNGNLKINMFVDKSSVEIYFQDGIEVASLKLYPKKDSFNLSLKSEEGKIKINSLSIWEM
NEVNYNE
>Mature_487_residues
MEFLNDEDINKKSKEYYNKTKDIWRDNFHFEPPFGLINDPNGLSYYKGEYYIFFQWNPYGCEHKNKHWGIVKTKNFKDFS
IPKIALTPKEYFDKDGCYSGGSIVKDGNLQVFYTGNVKGEKGERISYQCKGILEENGQLKKLGPVISTLPKGYTAHFRDP
YIWENNGYYYMILGIQTEKLKGRCVIYKSNDLDNWKFYGELKTSYEDFGFMWECPSFFKIDNKDILIFSPQGIKEEEFRY
QNIYQSGYLIGNLNYDTLEFKNNNEFKELDMGFDFYAPQVFKDEKGRNILIGWMGLPEKEEFHCSRENGWIFSLTMPREL
TLKNGVLHQNPIEEMRKLRGDKIVSLKNILIEDLNFNEVKENSYELKLDIERREASKIEINFMESNEEHTSLIYDFKKEI
CIIDRNSMINGEKGIRKFKLHSNGNLKINMFVDKSSVEIYFQDGIEVASLKLYPKKDSFNLSLKSEEGKIKINSLSIWEM
NEVNYNE

Specific function: Unknown

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=513, Percent_Identity=22.6120857699805, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 57512; Mature: 57512

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFLNDEDINKKSKEYYNKTKDIWRDNFHFEPPFGLINDPNGLSYYKGEYYIFFQWNPYG
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCEEEEEEECCCC
CEHKNKHWGIVKTKNFKDFSIPKIALTPKEYFDKDGCYSGGSIVKDGNLQVFYTGNVKGE
CCCCCCEECEEEECCCCCCCCCEEEECCHHHCCCCCCCCCCCEEECCCEEEEEECCCCCC
KGERISYQCKGILEENGQLKKLGPVISTLPKGYTAHFRDPYIWENNGYYYMILGIQTEKL
CCCEEEEEEEEEECCCCCEEHHCHHHHHCCCCCEECCCCCEEEECCCEEEEEEEEEECCC
KGRCVIYKSNDLDNWKFYGELKTSYEDFGFMWECPSFFKIDNKDILIFSPQGIKEEEFRY
CCEEEEEECCCCCCEEEEEEEECCCCCCCEEECCCCCEEECCCCEEEECCCCCCHHHHHH
QNIYQSGYLIGNLNYDTLEFKNNNEFKELDMGFDFYAPQVFKDEKGRNILIGWMGLPEKE
HHHHHCCEEEEECCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCH
EFHCSRENGWIFSLTMPRELTLKNGVLHQNPIEEMRKLRGDKIVSLKNILIEDLNFNEVK
HCEEECCCCEEEEEECCCEEEECCCCCCCCHHHHHHHHCCCEEEEEEHHEEECCCCCCCC
ENSYELKLDIERREASKIEINFMESNEEHTSLIYDFKKEICIIDRNSMINGEKGIRKFKL
CCCEEEEEEEECCCCCEEEEEEEECCCCHHHEEEECCCCEEEEECCCCCCCCCCCEEEEE
HSNGNLKINMFVDKSSVEIYFQDGIEVASLKLYPKKDSFNLSLKSEEGKIKINSLSIWEM
ECCCCEEEEEEEECCCEEEEEECCCEEEEEEEEECCCCEEEEEECCCCEEEEEEEEEEEE
NEVNYNE
CCCCCCC
>Mature Secondary Structure
MEFLNDEDINKKSKEYYNKTKDIWRDNFHFEPPFGLINDPNGLSYYKGEYYIFFQWNPYG
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCEEEEEEECCCC
CEHKNKHWGIVKTKNFKDFSIPKIALTPKEYFDKDGCYSGGSIVKDGNLQVFYTGNVKGE
CCCCCCEECEEEECCCCCCCCCEEEECCHHHCCCCCCCCCCCEEECCCEEEEEECCCCCC
KGERISYQCKGILEENGQLKKLGPVISTLPKGYTAHFRDPYIWENNGYYYMILGIQTEKL
CCCEEEEEEEEEECCCCCEEHHCHHHHHCCCCCEECCCCCEEEECCCEEEEEEEEEECCC
KGRCVIYKSNDLDNWKFYGELKTSYEDFGFMWECPSFFKIDNKDILIFSPQGIKEEEFRY
CCEEEEEECCCCCCEEEEEEEECCCCCCCEEECCCCCEEECCCCEEEECCCCCCHHHHHH
QNIYQSGYLIGNLNYDTLEFKNNNEFKELDMGFDFYAPQVFKDEKGRNILIGWMGLPEKE
HHHHHCCEEEEECCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCH
EFHCSRENGWIFSLTMPRELTLKNGVLHQNPIEEMRKLRGDKIVSLKNILIEDLNFNEVK
HCEEECCCCEEEEEECCCEEEECCCCCCCCHHHHHHHHCCCEEEEEEHHEEECCCCCCCC
ENSYELKLDIERREASKIEINFMESNEEHTSLIYDFKKEICIIDRNSMINGEKGIRKFKL
CCCEEEEEEEECCCCCEEEEEEEECCCCHHHEEEECCCCEEEEECCCCCCCCCCCEEEEE
HSNGNLKINMFVDKSSVEIYFQDGIEVASLKLYPKKDSFNLSLKSEEGKIKINSLSIWEM
ECCCCEEEEEEEECCCEEEEEECCCEEEEEEEEECCCCEEEEEECCCCEEEEEEEEEEEE
NEVNYNE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3100393; 7934828; 9384377; 3122206 [H]