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The map label for this gene is scrR [H]

Identifier: 18310515

GI number: 18310515

Start: 1795396

End: 1796376

Strand: Reverse

Name: scrR [H]

Synonym: CPE1533

Alternate gene names: 18310515

Gene position: 1796376-1795396 (Counterclockwise)

Preceding gene: 18310516

Following gene: 18310514

Centisome position: 59.26

GC content: 27.73

Gene sequence:

>981_bases
ATGATGAAAGTTACAATACAGGACATAGCTAATATGGTAGGAGTATCAAAAAGTACTGTATCAAGATACTTAAATGGTGG
TTATGTGAGTAATGAAAATACAGTTAAGATAAAAGAGGCTATAGAAAAAACTGGTTTTGAAACTAACTTTTTTGCTAAAA
GGTTAAAGTCTAAACAAAGTAAGTTAATAGGAATAATAGTTCCTAGAATAGACTCATTTACTGCAGGTAAAACATTAAAT
GGTATAAATAAAAAACTAGAAGAACAGGGTTATCAAGGAATAATATTAACAAGTGAATTTAACATAGAAAAGGAATTAGA
ACAAATTAATAAGTTATATAATCAAGGGGTAGATGGAATTATTATAATGTCTTTTGAAATTTCAAAAGAACATGTAAAAT
TAGCCAATAAACTTCCTATACCTATTTTGTTTTCAGGACAAAAAAATGATTATATAAATTATTTAGTTTTAGATGATGAA
AAGATAGGAAATATTTTAGGCACATATATAAAAGAACAAGGACATAAAGATATTGTGTTCTTAGGGGTTAGTGAAAGTGA
TAGAGAAGTTGGAATTCTTAGAAAAAAGGGATTTTGTGATGTTTTTAAAAAAGAGTGCAATATAAGTTTTGTAGAAACAG
ACTTTTCTTTTGATAAAGCATATGAAGCAGGAGAAGAGGTTTTAAAATATAATCCAACAGCTATTGTATGTGCAACAGAT
AATATAGCTCTTGGGCTTATGAGATATCTTCTTGAGAATGGAAAGCAAATACCAGAAGATATTTCCATTGCAGCCTTTGG
AGGATATGAGGTAAGTGCAATAACATATCCATCACTAACCACTGTAAAAATTGATTATAAATTATTTGGTGAAGAAATAG
CACTTAATATTTTAAGTTTGATAAATGGAGAGAAAAGTATAAATACTTGTGAAATTCCTTTAGAACTTATGGTAAGAGAA
AGTGTTAAAAGTTTAAAGTAG

Upstream 100 bases:

>100_bases
TGCTTTTAAATATTTCAAAATAGTCTTATATGGTTAAAAACTTCTAGATAATGATATAATTTATAATATAAGAAGATATA
TATCTTTATTTAGGAGGAAA

Downstream 100 bases:

>100_bases
GGTTTGCTTCACCGAATAATTTAAAGAAAGGTGAAGTAAATGGAGTTTTTAAATGATGAAGATATAAACAAAAAATCAAA
AGAATATTATAATAAAACTA

Product: sucrose operon repressor

Products: NA

Alternate protein names: Scr operon regulatory protein [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MMKVTIQDIANMVGVSKSTVSRYLNGGYVSNENTVKIKEAIEKTGFETNFFAKRLKSKQSKLIGIIVPRIDSFTAGKTLN
GINKKLEEQGYQGIILTSEFNIEKELEQINKLYNQGVDGIIIMSFEISKEHVKLANKLPIPILFSGQKNDYINYLVLDDE
KIGNILGTYIKEQGHKDIVFLGVSESDREVGILRKKGFCDVFKKECNISFVETDFSFDKAYEAGEEVLKYNPTAIVCATD
NIALGLMRYLLENGKQIPEDISIAAFGGYEVSAITYPSLTTVKIDYKLFGEEIALNILSLINGEKSINTCEIPLELMVRE
SVKSLK

Sequences:

>Translated_326_residues
MMKVTIQDIANMVGVSKSTVSRYLNGGYVSNENTVKIKEAIEKTGFETNFFAKRLKSKQSKLIGIIVPRIDSFTAGKTLN
GINKKLEEQGYQGIILTSEFNIEKELEQINKLYNQGVDGIIIMSFEISKEHVKLANKLPIPILFSGQKNDYINYLVLDDE
KIGNILGTYIKEQGHKDIVFLGVSESDREVGILRKKGFCDVFKKECNISFVETDFSFDKAYEAGEEVLKYNPTAIVCATD
NIALGLMRYLLENGKQIPEDISIAAFGGYEVSAITYPSLTTVKIDYKLFGEEIALNILSLINGEKSINTCEIPLELMVRE
SVKSLK
>Mature_326_residues
MMKVTIQDIANMVGVSKSTVSRYLNGGYVSNENTVKIKEAIEKTGFETNFFAKRLKSKQSKLIGIIVPRIDSFTAGKTLN
GINKKLEEQGYQGIILTSEFNIEKELEQINKLYNQGVDGIIIMSFEISKEHVKLANKLPIPILFSGQKNDYINYLVLDDE
KIGNILGTYIKEQGHKDIVFLGVSESDREVGILRKKGFCDVFKKECNISFVETDFSFDKAYEAGEEVLKYNPTAIVCATD
NIALGLMRYLLENGKQIPEDISIAAFGGYEVSAITYPSLTTVKIDYKLFGEEIALNILSLINGEKSINTCEIPLELMVRE
SVKSLK

Specific function: Negative regulator of scrB expression [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790689, Length=319, Percent_Identity=28.5266457680251, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787906, Length=284, Percent_Identity=30.9859154929577, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI48994940, Length=310, Percent_Identity=25.4838709677419, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=25.5255255255255, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790194, Length=305, Percent_Identity=25.2459016393443, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789068, Length=320, Percent_Identity=27.5, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786540, Length=333, Percent_Identity=27.3273273273273, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=26.625386996904, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790369, Length=340, Percent_Identity=25.8823529411765, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787580, Length=335, Percent_Identity=26.5671641791045, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786268, Length=282, Percent_Identity=25.177304964539, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1789202, Length=292, Percent_Identity=26.027397260274, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788474, Length=293, Percent_Identity=28.6689419795222, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36477; Mature: 36477

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKVTIQDIANMVGVSKSTVSRYLNGGYVSNENTVKIKEAIEKTGFETNFFAKRLKSKQS
CEEEEHHHHHHHHCCCHHHHHHHHCCCEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHC
KLIGIIVPRIDSFTAGKTLNGINKKLEEQGYQGIILTSEFNIEKELEQINKLYNQGVDGI
CEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEE
IIMSFEISKEHVKLANKLPIPILFSGQKNDYINYLVLDDEKIGNILGTYIKEQGHKDIVF
EEEEEECCHHHHHHHHCCCCEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEE
LGVSESDREVGILRKKGFCDVFKKECNISFVETDFSFDKAYEAGEEVLKYNPTAIVCATD
EECCCCCCCEEEEECCCHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHEECCCEEEEECC
NIALGLMRYLLENGKQIPEDISIAAFGGYEVSAITYPSLTTVKIDYKLFGEEIALNILSL
CHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEEEECCCEEEEEEEHHHHHHHHHHHHHHH
INGEKSINTCEIPLELMVRESVKSLK
HCCCCCCCEECCHHHHHHHHHHHHCC
>Mature Secondary Structure
MMKVTIQDIANMVGVSKSTVSRYLNGGYVSNENTVKIKEAIEKTGFETNFFAKRLKSKQS
CEEEEHHHHHHHHCCCHHHHHHHHCCCEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHC
KLIGIIVPRIDSFTAGKTLNGINKKLEEQGYQGIILTSEFNIEKELEQINKLYNQGVDGI
CEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEE
IIMSFEISKEHVKLANKLPIPILFSGQKNDYINYLVLDDEKIGNILGTYIKEQGHKDIVF
EEEEEECCHHHHHHHHCCCCEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEE
LGVSESDREVGILRKKGFCDVFKKECNISFVETDFSFDKAYEAGEEVLKYNPTAIVCATD
EECCCCCCCEEEEECCCHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHEECCCEEEEECC
NIALGLMRYLLENGKQIPEDISIAAFGGYEVSAITYPSLTTVKIDYKLFGEEIALNILSL
CHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEEEECCCEEEEEEEHHHHHHHHHHHHHHH
INGEKSINTCEIPLELMVRESVKSLK
HCCCCCCCEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550467 [H]