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The map label for this gene is ydjE [H]

Identifier: 18310513

GI number: 18310513

Start: 1792953

End: 1793900

Strand: Reverse

Name: ydjE [H]

Synonym: CPE1531

Alternate gene names: 18310513

Gene position: 1793900-1792953 (Counterclockwise)

Preceding gene: 18310514

Following gene: 18310512

Centisome position: 59.18

GC content: 30.38

Gene sequence:

>948_bases
ATGAATAAAGTATTTTGTATAGGAGAGTTATTAATAGATTTTATAGGAAAAGATATAGGAAAAGGTCTTAAAAATGGAGT
AAACTTTGAGAAAAAGGCTGGTGGAGCTCCAGCAAATGTTGGAGCAGCAGTTTGTAAGCTTGGAGGAGAATCTTATTTTT
TAGGACAAGTTGGTAATGACTCTTTTGGAGAATTTCTAGTTGATATGCTAAAAAATATAGGTATAAATACTGAAATGACT
AAAATGGATGGATATACAACCTTAGCATTTGTAGCTATTGATGAAAAGGGAGAAAGAGATTTTGAGTTTCATAGAGGTAG
TGATGGAGAATACTCATTTAATAATATTGATTTAAGTAAAATAGAAAAGGATGATATAATTCATTTTGGTTCAGCTACGG
GATTTTTAAAAGGTGAACTTAAGAACACTTATTTTAAGCTTTTAGAGATTGGACGTAAAAATGGAAACTTTATATCTTTT
GATCCAAACTATAGAGATATGCTTATTAAAGAAGAAGGAATTGAAGAGTTTAAAGAAGACTGTAAGAAATTTATAAGCTC
TTGTGATTTTTTAAAGCTTAGTGATGAGGAGATAAAGCTTTTAACTGAGGAAGAAGATTTAGAAAGAGGAGTTGAAAAAC
TTCATTCTTTAGGAGCAAAAATAATAGCTGTTACACTAGGAGGAAAAGGAACTCTTTTAAGTAATGGCATTCATTCAGAG
ATAGTTCCATCAATAAAAATTAATCAAGTAGATTCAACTGGGGCAGGAGATGCTTTCGTTGGGGCTGTTCTTAAGAAAGT
AAGCGAATTAAAAGATAAGAAGAATTTAGATTTAAATTCATGGAGAGAAATTATAAGTTATGGAAATAAGGTTGGAGCAT
TGACATGCACAAATTATGGTGCTATTGATGCTATTCCAAATGAAGAAGATTTAATGAAATATATATAG

Upstream 100 bases:

>100_bases
TACCCTAAAAAAGATAGCTTTAATTTAAGTTTAAAATCAGAAGAGGGTAAAATTAAAATAAATTCCTTGAGTATATGGGA
GATGAATGAGGTGAATTATA

Downstream 100 bases:

>100_bases
TTTATTTGAAAGCATATGAGAATTTTTAGGAACCTATTAAAATCCTTTAGTTCTCATATGTTTTTTTATTTTAGTATATT
TTAGATTAGAGTTTTATCCT

Product: fructokinase

Products: ADP; D-fructose 6-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MNKVFCIGELLIDFIGKDIGKGLKNGVNFEKKAGGAPANVGAAVCKLGGESYFLGQVGNDSFGEFLVDMLKNIGINTEMT
KMDGYTTLAFVAIDEKGERDFEFHRGSDGEYSFNNIDLSKIEKDDIIHFGSATGFLKGELKNTYFKLLEIGRKNGNFISF
DPNYRDMLIKEEGIEEFKEDCKKFISSCDFLKLSDEEIKLLTEEEDLERGVEKLHSLGAKIIAVTLGGKGTLLSNGIHSE
IVPSIKINQVDSTGAGDAFVGAVLKKVSELKDKKNLDLNSWREIISYGNKVGALTCTNYGAIDAIPNEEDLMKYI

Sequences:

>Translated_315_residues
MNKVFCIGELLIDFIGKDIGKGLKNGVNFEKKAGGAPANVGAAVCKLGGESYFLGQVGNDSFGEFLVDMLKNIGINTEMT
KMDGYTTLAFVAIDEKGERDFEFHRGSDGEYSFNNIDLSKIEKDDIIHFGSATGFLKGELKNTYFKLLEIGRKNGNFISF
DPNYRDMLIKEEGIEEFKEDCKKFISSCDFLKLSDEEIKLLTEEEDLERGVEKLHSLGAKIIAVTLGGKGTLLSNGIHSE
IVPSIKINQVDSTGAGDAFVGAVLKKVSELKDKKNLDLNSWREIISYGNKVGALTCTNYGAIDAIPNEEDLMKYI
>Mature_315_residues
MNKVFCIGELLIDFIGKDIGKGLKNGVNFEKKAGGAPANVGAAVCKLGGESYFLGQVGNDSFGEFLVDMLKNIGINTEMT
KMDGYTTLAFVAIDEKGERDFEFHRGSDGEYSFNNIDLSKIEKDDIIHFGSATGFLKGELKNTYFKLLEIGRKNGNFISF
DPNYRDMLIKEEGIEEFKEDCKKFISSCDFLKLSDEEIKLLTEEEDLERGVEKLHSLGAKIIAVTLGGKGTLLSNGIHSE
IVPSIKINQVDSTGAGDAFVGAVLKKVSELKDKKNLDLNSWREIISYGNKVGALTCTNYGAIDAIPNEEDLMKYI

Specific function: Unknown

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI87081971, Length=321, Percent_Identity=23.6760124610592, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082281, Length=283, Percent_Identity=24.0282685512367, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790193, Length=298, Percent_Identity=22.4832214765101, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.4

Molecular weight: Translated: 34665; Mature: 34665

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVFCIGELLIDFIGKDIGKGLKNGVNFEKKAGGAPANVGAAVCKLGGESYFLGQVGND
CCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHCCCCEEEEEECCCC
SFGEFLVDMLKNIGINTEMTKMDGYTTLAFVAIDEKGERDFEFHRGSDGEYSFNNIDLSK
HHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEECCCCCCCCCEECCCCCCEEECCCCHHH
IEKDDIIHFGSATGFLKGELKNTYFKLLEIGRKNGNFISFDPNYRDMLIKEEGIEEFKED
CCCCCEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHH
CKKFISSCDFLKLSDEEIKLLTEEEDLERGVEKLHSLGAKIIAVTLGGKGTLLSNGIHSE
HHHHHHCCCCEEECCCHHEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCEECCCCHHH
IVPSIKINQVDSTGAGDAFVGAVLKKVSELKDKKNLDLNSWREIISYGNKVGALTCTNYG
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCC
AIDAIPNEEDLMKYI
CCCCCCCHHHHHHCC
>Mature Secondary Structure
MNKVFCIGELLIDFIGKDIGKGLKNGVNFEKKAGGAPANVGAAVCKLGGESYFLGQVGND
CCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHCCCCEEEEEECCCC
SFGEFLVDMLKNIGINTEMTKMDGYTTLAFVAIDEKGERDFEFHRGSDGEYSFNNIDLSK
HHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEECCCCCCCCCEECCCCCCEEECCCCHHH
IEKDDIIHFGSATGFLKGELKNTYFKLLEIGRKNGNFISFDPNYRDMLIKEEGIEEFKED
CCCCCEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHH
CKKFISSCDFLKLSDEEIKLLTEEEDLERGVEKLHSLGAKIIAVTLGGKGTLLSNGIHSE
HHHHHHCCCCEEECCCHHEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCEECCCCHHH
IVPSIKINQVDSTGAGDAFVGAVLKKVSELKDKKNLDLNSWREIISYGNKVGALTCTNYG
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCC
AIDAIPNEEDLMKYI
CCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-fructose

Specific reaction: ATP + D-fructose = ADP + D-fructose 6-phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9455482; 9384377 [H]