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The map label for this gene is alf2 [H]

Identifier: 18310332

GI number: 18310332

Start: 1594317

End: 1595183

Strand: Direct

Name: alf2 [H]

Synonym: CPE1350

Alternate gene names: 18310332

Gene position: 1594317-1595183 (Clockwise)

Preceding gene: 18310331

Following gene: 18310334

Centisome position: 52.59

GC content: 35.87

Gene sequence:

>867_bases
ATGGCATTAGTTAACGCAAAAGAAATGTTAAATAAAGCAAGAGAAGGAAAATACGCTGTTGGTCAATTCAACATAAACAA
CTTAGAATGGACAAAAGCTATATTATTAACTGCTCAAGAAAATAACTCACCAGTTATATTAGGAGTATCAGAAGGTGCTG
CTAAATACATGTGTGGATTCAAAACAATAGTTGGAATGGTTAACGGAATGTTAGAAGAATTAAAAATAACTGTTCCTGTA
GCATTACACTTAGATCACGGTAGCTACCAAGGAGCTATAGATGCTATGGATGCTGGATTCTCATCAGTAATGTTCGATGG
ATCACACTACTCAATCGAAGAAAACATAGTTAAAACTAAAGAAATAATCAACTTAGCTGCTGCTAAAAACGTATCAGTTG
AAGCTGAAGTTGGATCAATCGGTGGAGAAGAAGACGGTGTTGTTGGAGCTGGTGAAATCGCTGATCCTGCTGAATGTAAA
CAAATCGCTGAATTAGGAGTTACTATGTTAGCTGCTGGTATCGGAAACATTCACGGAAAATACCCTGCAAACTGGGCTGG
ATTAAACTTCGAAGCTTTAGCTAACATTAAAGCTGCTACTGGAGATATGCCTTTAGTATTACACGGTGGTACTGGAATCC
CTTCAGATATGATCGCAGAAGCTATATCATTAGGAGTATCAAAAATAAATGTTAATACTGAGTGTCAATTATCATTTGCT
GAAGCTACTCGTAAATATATAGAAGCTGGAAAAGACTTAGAAGGAAAAGGATTTGACCCAAGAAAATTATTAAATCCTGG
ATTCGAAGCTATAAAAGCTACAGTTAAAGAAAAAATGGAATTATTCGGTTCAGTAAACAGAGCTTAA

Upstream 100 bases:

>100_bases
ATAATGTTATTGAAAGGGGCAAGTCCCGCATGGACTTTTTTTGTCCCTATATATAAATCTAATAAAAGTTAATGAAAGAT
TTTGAAAGGTGGTAAACAAA

Downstream 100 bases:

>100_bases
TAGTTTTAAATAATAAAAAAGAGATTAGATTTTCTAATCTCTTTTTTTATTTCTCTATATAATTTTTAATATTTTTTATA
ACCATATTTATGCTTCCATC

Product: fructose-1,6-bisphosphate aldolase, class II

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MALVNAKEMLNKAREGKYAVGQFNINNLEWTKAILLTAQENNSPVILGVSEGAAKYMCGFKTIVGMVNGMLEELKITVPV
ALHLDHGSYQGAIDAMDAGFSSVMFDGSHYSIEENIVKTKEIINLAAAKNVSVEAEVGSIGGEEDGVVGAGEIADPAECK
QIAELGVTMLAAGIGNIHGKYPANWAGLNFEALANIKAATGDMPLVLHGGTGIPSDMIAEAISLGVSKINVNTECQLSFA
EATRKYIEAGKDLEGKGFDPRKLLNPGFEAIKATVKEKMELFGSVNRA

Sequences:

>Translated_288_residues
MALVNAKEMLNKAREGKYAVGQFNINNLEWTKAILLTAQENNSPVILGVSEGAAKYMCGFKTIVGMVNGMLEELKITVPV
ALHLDHGSYQGAIDAMDAGFSSVMFDGSHYSIEENIVKTKEIINLAAAKNVSVEAEVGSIGGEEDGVVGAGEIADPAECK
QIAELGVTMLAAGIGNIHGKYPANWAGLNFEALANIKAATGDMPLVLHGGTGIPSDMIAEAISLGVSKINVNTECQLSFA
EATRKYIEAGKDLEGKGFDPRKLLNPGFEAIKATVKEKMELFGSVNRA
>Mature_287_residues
ALVNAKEMLNKAREGKYAVGQFNINNLEWTKAILLTAQENNSPVILGVSEGAAKYMCGFKTIVGMVNGMLEELKITVPVA
LHLDHGSYQGAIDAMDAGFSSVMFDGSHYSIEENIVKTKEIINLAAAKNVSVEAEVGSIGGEEDGVVGAGEIADPAECKQ
IAELGVTMLAAGIGNIHGKYPANWAGLNFEALANIKAATGDMPLVLHGGTGIPSDMIAEAISLGVSKINVNTECQLSFAE
ATRKYIEAGKDLEGKGFDPRKLLNPGFEAIKATVKEKMELFGSVNRA

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis [H]

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789526, Length=292, Percent_Identity=36.3013698630137, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI87082041, Length=293, Percent_Identity=35.1535836177474, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1788072, Length=292, Percent_Identity=34.9315068493151, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1789293, Length=341, Percent_Identity=25.8064516129032, Blast_Score=85, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6322790, Length=339, Percent_Identity=28.9085545722714, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011289
- InterPro:   IPR000771 [H]

Pfam domain/function: PF01116 F_bP_aldolase [H]

EC number: =4.1.2.13 [H]

Molecular weight: Translated: 30432; Mature: 30301

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00806 ALDOLASE_CLASS_II_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVNAKEMLNKAREGKYAVGQFNINNLEWTKAILLTAQENNSPVILGVSEGAAKYMCGF
CCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEEEEEEEECCCCCEEEEECCCCHHHHHHH
KTIVGMVNGMLEELKITVPVALHLDHGSYQGAIDAMDAGFSSVMFDGSHYSIEENIVKTK
HHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCHHHHHCCCHHEEECCCCCCHHHHHHHHH
EIINLAAAKNVSVEAEVGSIGGEEDGVVGAGEIADPAECKQIAELGVTMLAAGIGNIHGK
HHHHHHHHCCCEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
YPANWAGLNFEALANIKAATGDMPLVLHGGTGIPSDMIAEAISLGVSKINVNTECQLSFA
CCCCCCCCCHHHHHCCEECCCCCCEEEECCCCCCHHHHHHHHHHCCEEEECCCCEEEEHH
EATRKYIEAGKDLEGKGFDPRKLLNPGFEAIKATVKEKMELFGSVNRA
HHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALVNAKEMLNKAREGKYAVGQFNINNLEWTKAILLTAQENNSPVILGVSEGAAKYMCGF
CCCCHHHHHHHHHCCCEEEEEEECCCCEEEEEEEEEEECCCCCEEEEECCCCHHHHHHH
KTIVGMVNGMLEELKITVPVALHLDHGSYQGAIDAMDAGFSSVMFDGSHYSIEENIVKTK
HHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCHHHHHCCCHHEEECCCCCCHHHHHHHHH
EIINLAAAKNVSVEAEVGSIGGEEDGVVGAGEIADPAECKQIAELGVTMLAAGIGNIHGK
HHHHHHHHCCCEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
YPANWAGLNFEALANIKAATGDMPLVLHGGTGIPSDMIAEAISLGVSKINVNTECQLSFA
CCCCCCCCCHHHHHCCEECCCCCCEEEECCCCCCHHHHHHHHHHCCEEEECCCCEEEEHH
EATRKYIEAGKDLEGKGFDPRKLLNPGFEAIKATVKEKMELFGSVNRA
HHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]