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The map label for this gene is pgk

Identifier: 18310285

GI number: 18310285

Start: 1542371

End: 1543564

Strand: Reverse

Name: pgk

Synonym: CPE1303

Alternate gene names: 18310285

Gene position: 1543564-1542371 (Counterclockwise)

Preceding gene: 18310286

Following gene: 18310284

Centisome position: 50.92

GC content: 35.59

Gene sequence:

>1194_bases
ATGAACTTCAATAAAAAGACTATAGAAGACGTTCAAGTAAAAGGTAAAAAAGTATTAGTAAGATGCGACTTTAACGTTCC
ATTAAAAGATGGTGTTATAACTGATGAAAACAGATTAAACGGAGCTATGCCAACTATAAAATACTTAGTTGATAATGGCG
CTAAAGTAATCCTTTGCTCACATATGGGTAAACCAAAAGGAGAAGCTAAACCAGAATTCTCATTAGCACCTGTAGCTAAA
AGATTAAGCGAAATGCTTGGTAAAGAAGTAGTATTTGCTGCTGACGACAACGTTGTTGGAGAAAATGCTAAAAAAGCTGT
AGCTGAAATGAAAGATGGAGACGTAGTTTTACTTCAAAATACAAGATACAGAAAAGAAGAAACTAAAAATGGTGAAGAGT
TATCAAAAGAATTAGCTAGCTTAGCTGAAATGTTTGTTAACGATGCATTCGGTACTGCTCACAGAGCTCACTGCTCAACA
GTTGGAGTAACTGAATACTTAAAACCAGCAGTATGTGGATACTTAATCCAAAAAGAGCTTAAGTTCTTAGGAGATGCTGT
AGAAACTCCAGAAAGACCATTCGTTGCTATCTTAGGTGGAGCTAAAGTTTCAGATAAGATAAACGTTATAAACAACCTTT
TAGAAAAAGTTGATACTTTAATAATAGGTGGAGGAATGGCTTACACATTCTTAAAAGCTCAAGGATATACTGTAGGAAGT
TCATTAGTAGAAGAAGATAAAGTTGAATACGCTAAAGAAATGTTAGCTAAAGCTGAGGAAAAAGGTGTTAAATTATTATT
ACCTGTAGACCACAGAGTAGCTAAAGAATTCAAAGATGTTGAAGCTGTAGTTACAGAAGATCAAAACATCGCTGAAGGAT
TCATGGGATTAGATATCGGACCAAAAACTGAAGCTATCTACGCTGAAGCTATAAAAGATGCTAAAACAGTTATCTGGAAC
GGACCAATGGGAGTATTCGAATTCGAAAACTTCAACAAAGGAACAATAGCTGTTGCTAAAGCTATGGCTGAAGCAGATGC
TACTACTATAATCGGTGGTGGAGATTCAGCTGCTGCTGTAAACATCTTAGGATTCGGTGACAAAATGTCTCACATCTCAA
CTGGTGGTGGAGCTTCATTAGAATTCTTAGAAGGAAAAGTATTACCAGGAATAGCTGCTTTAAACGATAAATAA

Upstream 100 bases:

>100_bases
GTTTTATAGTTAAAGCTATGAGGCCAGACTACTTTAACATATAAATGATTTTTGTTTATATTAAAGGTAATTATAAAATA
TTATTTAAGAGGTGAATATA

Downstream 100 bases:

>100_bases
TATTATAAACCTTAAAGTAGATATTTAGATTGAAGTATAAAGATTGTTGTTTTATAAAAAAATAAGACTAAGTTCAATGA
ATTTAAATTTTCATTGAATC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNFNKKTIEDVQVKGKKVLVRCDFNVPLKDGVITDENRLNGAMPTIKYLVDNGAKVILCSHMGKPKGEAKPEFSLAPVAK
RLSEMLGKEVVFAADDNVVGENAKKAVAEMKDGDVVLLQNTRYRKEETKNGEELSKELASLAEMFVNDAFGTAHRAHCST
VGVTEYLKPAVCGYLIQKELKFLGDAVETPERPFVAILGGAKVSDKINVINNLLEKVDTLIIGGGMAYTFLKAQGYTVGS
SLVEEDKVEYAKEMLAKAEEKGVKLLLPVDHRVAKEFKDVEAVVTEDQNIAEGFMGLDIGPKTEAIYAEAIKDAKTVIWN
GPMGVFEFENFNKGTIAVAKAMAEADATTIIGGGDSAAAVNILGFGDKMSHISTGGGASLEFLEGKVLPGIAALNDK

Sequences:

>Translated_397_residues
MNFNKKTIEDVQVKGKKVLVRCDFNVPLKDGVITDENRLNGAMPTIKYLVDNGAKVILCSHMGKPKGEAKPEFSLAPVAK
RLSEMLGKEVVFAADDNVVGENAKKAVAEMKDGDVVLLQNTRYRKEETKNGEELSKELASLAEMFVNDAFGTAHRAHCST
VGVTEYLKPAVCGYLIQKELKFLGDAVETPERPFVAILGGAKVSDKINVINNLLEKVDTLIIGGGMAYTFLKAQGYTVGS
SLVEEDKVEYAKEMLAKAEEKGVKLLLPVDHRVAKEFKDVEAVVTEDQNIAEGFMGLDIGPKTEAIYAEAIKDAKTVIWN
GPMGVFEFENFNKGTIAVAKAMAEADATTIIGGGDSAAAVNILGFGDKMSHISTGGGASLEFLEGKVLPGIAALNDK
>Mature_397_residues
MNFNKKTIEDVQVKGKKVLVRCDFNVPLKDGVITDENRLNGAMPTIKYLVDNGAKVILCSHMGKPKGEAKPEFSLAPVAK
RLSEMLGKEVVFAADDNVVGENAKKAVAEMKDGDVVLLQNTRYRKEETKNGEELSKELASLAEMFVNDAFGTAHRAHCST
VGVTEYLKPAVCGYLIQKELKFLGDAVETPERPFVAILGGAKVSDKINVINNLLEKVDTLIIGGGMAYTFLKAQGYTVGS
SLVEEDKVEYAKEMLAKAEEKGVKLLLPVDHRVAKEFKDVEAVVTEDQNIAEGFMGLDIGPKTEAIYAEAIKDAKTVIWN
GPMGVFEFENFNKGTIAVAKAMAEADATTIIGGGDSAAAVNILGFGDKMSHISTGGGASLEFLEGKVLPGIAALNDK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=418, Percent_Identity=47.8468899521531, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI31543397, Length=420, Percent_Identity=45.2380952380952, Blast_Score=353, Evalue=2e-97,
Organism=Escherichia coli, GI1789294, Length=394, Percent_Identity=44.9238578680203, Blast_Score=325, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17508823, Length=417, Percent_Identity=48.2014388489209, Blast_Score=373, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6319857, Length=415, Percent_Identity=46.0240963855422, Blast_Score=354, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24581203, Length=416, Percent_Identity=45.9134615384615, Blast_Score=341, Evalue=5e-94,
Organism=Drosophila melanogaster, GI17136394, Length=416, Percent_Identity=44.2307692307692, Blast_Score=331, Evalue=5e-91,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_CLOP1 (Q0TQY7)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_695953.1
- ProteinModelPortal:   Q0TQY7
- SMR:   Q0TQY7
- STRING:   Q0TQY7
- GeneID:   4203510
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_1510
- TIGR:   CPF_1510
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   CPER195103:CPF_1510-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42680; Mature: 42680

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 38-38 BINDING 122-122 BINDING 155-155 BINDING 206-206 BINDING 296-296 BINDING 327-327

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFNKKTIEDVQVKGKKVLVRCDFNVPLKDGVITDENRLNGAMPTIKYLVDNGAKVILCS
CCCCHHHHHHHHCCCEEEEEEECCCCCCCCCEECCCCCCCCCCHHHHHHHCCCCEEEEEE
HMGKPKGEAKPEFSLAPVAKRLSEMLGKEVVFAADDNVVGENAKKAVAEMKDGDVVLLQN
CCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEC
TRYRKEETKNGEELSKELASLAEMFVNDAFGTAHRAHCSTVGVTEYLKPAVCGYLIQKEL
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHH
KFLGDAVETPERPFVAILGGAKVSDKINVINNLLEKVDTLIIGGGMAYTFLKAQGYTVGS
HHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCH
SLVEEDKVEYAKEMLAKAEEKGVKLLLPVDHRVAKEFKDVEAVVTEDQNIAEGFMGLDIG
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
PKTEAIYAEAIKDAKTVIWNGPMGVFEFENFNKGTIAVAKAMAEADATTIIGGGDSAAAV
CCCHHHHHHHHHCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCEEE
NILGFGDKMSHISTGGGASLEFLEGKVLPGIAALNDK
EEEECCCHHHHCCCCCCCEEHEECCCCCCCCCCCCCC
>Mature Secondary Structure
MNFNKKTIEDVQVKGKKVLVRCDFNVPLKDGVITDENRLNGAMPTIKYLVDNGAKVILCS
CCCCHHHHHHHHCCCEEEEEEECCCCCCCCCEECCCCCCCCCCHHHHHHHCCCCEEEEEE
HMGKPKGEAKPEFSLAPVAKRLSEMLGKEVVFAADDNVVGENAKKAVAEMKDGDVVLLQN
CCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEC
TRYRKEETKNGEELSKELASLAEMFVNDAFGTAHRAHCSTVGVTEYLKPAVCGYLIQKEL
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHH
KFLGDAVETPERPFVAILGGAKVSDKINVINNLLEKVDTLIIGGGMAYTFLKAQGYTVGS
HHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCH
SLVEEDKVEYAKEMLAKAEEKGVKLLLPVDHRVAKEFKDVEAVVTEDQNIAEGFMGLDIG
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
PKTEAIYAEAIKDAKTVIWNGPMGVFEFENFNKGTIAVAKAMAEADATTIIGGGDSAAAV
CCCHHHHHHHHHCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCEEE
NILGFGDKMSHISTGGGASLEFLEGKVLPGIAALNDK
EEEECCCHHHHCCCCCCCEEHEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA