Click here to switch to the map view.

The map label for this gene is tpiA

Identifier: 18310284

GI number: 18310284

Start: 1541381

End: 1542127

Strand: Reverse

Name: tpiA

Synonym: CPE1302

Alternate gene names: 18310284

Gene position: 1542127-1541381 (Counterclockwise)

Preceding gene: 18310285

Following gene: 18310283

Centisome position: 50.87

GC content: 37.35

Gene sequence:

>747_bases
ATGAGAACTCCAATAATCGCAGGAAACTGGAAAATGCACTATACAATAGACGAGGCTGTTAAATTAGTTGAAGAATTAAA
GCCATTAGTTAAAGATGCTAAATGTGAAGTTGTTGTTTGCCCAACTTTTGTTTGCTTAGATGCAGTTAAAAAAGCTGTTG
AAGGAACAAACATAAAAGTAGGTGCTCAAAACATGCACTTTGAAGAAAAAGGAGCATTCACTGGAGAAATCGCTCCAAGA
ATGTTAGAAGCTATGAACATAGATTACGTTATAATTGGACATAGTGAAAGAAGAGAATACTTCAACGAAACAGATGAAAC
TTGCAACAAAAAAGTTAAAGCTGCTTTCGCACACAACTTAACTCCAATCCTTTGCTGTGGAGAAACTTTAGAGCAAAGAG
AAAACGGAACTACTAACGACGTTATAAAAGCTCAAATCACTGCTGATTTAGAAGGATTAACTAAAGAGCAAGCTGAGAAA
GTTGTTATAGCTTACGAACCAATCTGGGCTATCGGAACTGGAAAAACTGCTACTTCAGACCAAGCTAATGAAACAATAGC
TGCTATAAGAGCTATGGTTGCTGAAATGTTCGGACAAGAAGTTGCTGATAAAGTAAGAATCCAATACGGTGGATCAGTTA
AGCCTAACACAATAGCAGAGCAAATGGCTAAATCAGACATCGATGGTGCTTTAGTTGGTGGAGCTAGCTTAGTTGCAGCT
GACTTTGCTCAAATCGTAAACTACTAA

Upstream 100 bases:

>100_bases
ATTTAATATTTTCAAAGCTTTTAGAGAAATAATATATAGTAGGGGATTGAGCCTTATATATATTATTTAATATTTAAAAT
ATTAATAGAGGTGAATATAG

Downstream 100 bases:

>100_bases
TAAATATTAGTAATTAAATAGTAGCAATAGGCAAGTAATACCAATTGTCTATTGCTATTGTTTTTTAATTAGGTACACAT
TATAATAAATGTATAAATAG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRTPIIAGNWKMHYTIDEAVKLVEELKPLVKDAKCEVVVCPTFVCLDAVKKAVEGTNIKVGAQNMHFEEKGAFTGEIAPR
MLEAMNIDYVIIGHSERREYFNETDETCNKKVKAAFAHNLTPILCCGETLEQRENGTTNDVIKAQITADLEGLTKEQAEK
VVIAYEPIWAIGTGKTATSDQANETIAAIRAMVAEMFGQEVADKVRIQYGGSVKPNTIAEQMAKSDIDGALVGGASLVAA
DFAQIVNY

Sequences:

>Translated_248_residues
MRTPIIAGNWKMHYTIDEAVKLVEELKPLVKDAKCEVVVCPTFVCLDAVKKAVEGTNIKVGAQNMHFEEKGAFTGEIAPR
MLEAMNIDYVIIGHSERREYFNETDETCNKKVKAAFAHNLTPILCCGETLEQRENGTTNDVIKAQITADLEGLTKEQAEK
VVIAYEPIWAIGTGKTATSDQANETIAAIRAMVAEMFGQEVADKVRIQYGGSVKPNTIAEQMAKSDIDGALVGGASLVAA
DFAQIVNY
>Mature_248_residues
MRTPIIAGNWKMHYTIDEAVKLVEELKPLVKDAKCEVVVCPTFVCLDAVKKAVEGTNIKVGAQNMHFEEKGAFTGEIAPR
MLEAMNIDYVIIGHSERREYFNETDETCNKKVKAAFAHNLTPILCCGETLEQRENGTTNDVIKAQITADLEGLTKEQAEK
VVIAYEPIWAIGTGKTATSDQANETIAAIRAMVAEMFGQEVADKVRIQYGGSVKPNTIAEQMAKSDIDGALVGGASLVAA
DFAQIVNY

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=248, Percent_Identity=40.7258064516129, Blast_Score=168, Evalue=4e-42,
Organism=Homo sapiens, GI4507645, Length=248, Percent_Identity=40.7258064516129, Blast_Score=168, Evalue=4e-42,
Organism=Escherichia coli, GI1790353, Length=247, Percent_Identity=46.1538461538462, Blast_Score=199, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17536593, Length=247, Percent_Identity=42.914979757085, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6320255, Length=246, Percent_Identity=40.2439024390244, Blast_Score=177, Evalue=1e-45,
Organism=Drosophila melanogaster, GI28572008, Length=247, Percent_Identity=42.5101214574899, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI28572006, Length=247, Percent_Identity=42.5101214574899, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=42.5101214574899, Blast_Score=183, Evalue=8e-47,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_CLOP1 (Q0TQY8)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_695952.1
- ProteinModelPortal:   Q0TQY8
- SMR:   Q0TQY8
- STRING:   Q0TQY8
- GeneID:   4201860
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_1509
- TIGR:   CPF_1509
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   CPER195103:CPF_1509-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27058; Mature: 27058

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 94-94 ACT_SITE 166-166 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTPIIAGNWKMHYTIDEAVKLVEELKPLVKDAKCEVVVCPTFVCLDAVKKAVEGTNIKV
CCCCEEECCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHCCCCEEE
GAQNMHFEEKGAFTGEIAPRMLEAMNIDYVIIGHSERREYFNETDETCNKKVKAAFAHNL
CCCCCCCHHCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHCHHHHHHHHHHHHHHHHCC
TPILCCGETLEQRENGTTNDVIKAQITADLEGLTKEQAEKVVIAYEPIWAIGTGKTATSD
CEEEECCHHHHHHCCCCCCCEEEEEEHHHHHHHCHHHHCEEEEEECCEEEEECCCCCCCC
QANETIAAIRAMVAEMFGQEVADKVRIQYGGSVKPNTIAEQMAKSDIDGALVGGASLVAA
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCEEECCHHHHHH
DFAQIVNY
HHHHHHCC
>Mature Secondary Structure
MRTPIIAGNWKMHYTIDEAVKLVEELKPLVKDAKCEVVVCPTFVCLDAVKKAVEGTNIKV
CCCCEEECCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHCCCCEEE
GAQNMHFEEKGAFTGEIAPRMLEAMNIDYVIIGHSERREYFNETDETCNKKVKAAFAHNL
CCCCCCCHHCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHCHHHHHHHHHHHHHHHHCC
TPILCCGETLEQRENGTTNDVIKAQITADLEGLTKEQAEKVVIAYEPIWAIGTGKTATSD
CEEEECCHHHHHHCCCCCCCEEEEEEHHHHHHHCHHHHCEEEEEECCEEEEECCCCCCCC
QANETIAAIRAMVAEMFGQEVADKVRIQYGGSVKPNTIAEQMAKSDIDGALVGGASLVAA
HHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCEEECCHHHHHH
DFAQIVNY
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA