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The map label for this gene is miaB

Identifier: 18310075

GI number: 18310075

Start: 1294599

End: 1295942

Strand: Direct

Name: miaB

Synonym: CPE1093

Alternate gene names: 18310075

Gene position: 1294599-1295942 (Clockwise)

Preceding gene: 18310074

Following gene: 18310076

Centisome position: 42.71

GC content: 30.28

Gene sequence:

>1344_bases
ATGACATTAGAAAATAACATGGATAAAAAACTCTTCTGTATTTCTACATACGGATGCCAAATGAATGAAGAAGATTCAGA
AAAATTATCAGGGATGTTAAAATCTCAAGGATATGAAAGAACTGAAAATAAAGAAGAGGCTTCTATTATAATCTTTAATA
CATGTTGTGTAAGAGAAAATGCTGAAAATAAGGTTTTCGGTAATCTTGGACAACTTAAGCAATTAAAAAAGAAAAATCCT
AACTTAGTAATAGCAATTTGTGGATGTATGATGCAACAAGTAGGTATGGCAGACAAAGTCTTAAAAACTTTCCCTTATGT
TGACATAATATTTGGAACACATAATGCCCATAAATTCCCTGAATACCTACATAGAGTACTTCAAGAAGGTGTTCAAGTTA
AGGAAATATTAAACAAAGAAGAGGGAATCGTTGAAGGATTACCTATAGATAGAAAGAGTGATGTTAAAGCATTCGTTACT
ATCATGTATGGATGTAACAATTTCTGTACATACTGTATAGTTCCTTACGTTAGAGGAAGAGAAAGAAGTAGAAAATCTGA
GGATATCATAAAAGAAATAGAAGAATTAGTATCTCAAGGATATAAAGAAATAACTTTATTAGGACAAAACGTAAACTCAT
ATGGAAAAGGCTTAGAAGAAGATATAGATTTTGCTGGTCTTTTAAGAAAGGTTAATGAAGTTAAGGGGTTAGAGAGAGTT
AGATTTATGACTTCTCATCCTAAGGATTTAAGTGATGATGTTATCATGGCTATAAAAGAATGTGATAAGCTTTGTGAACA
AGTTCACTTACCAGTTCAAAGTGGATCATCAAGAATATTAAAAGAGATGAATAGACACTATGATAGAGAGTACTACTTAG
ATTTAGTTAAGAAAATAAAATCTGAAATACCAGATGTTACTTTAACAACTGATATAATCATTGGATTCCCAGGAGAAACT
GAAGAGGACTTCTTAGATACATTAAGTCTTTGTGAAGAAGTTGGATATGATTCAGCATTCACATTTATCTATTCAAGAAG
AAATCATACTCCTGCTGACAAAATGGAAAATCAAATTCCAGATGACATAAAGCATGATAGATTCAATAGATTAGTTGAAG
CTATAAACAAAAAGGTTGTAATAAAAAATAAAGAATATGAAGGTAAAGTAGTTGAAGTTCTTGTTGAAGGACCAAGTAAG
AATGATGAAACTAAATTAACTGGAAGAACTAGAAATGGTAAATTAGTTAACTTTGCTGGTGATGAAAAACTAGTAGGTGA
ATTAGTTAACTTAAAAATAGTTAGAGCACAACCATTCTCATTAATTGGTGAAATAGTAGAATAA

Upstream 100 bases:

>100_bases
CTTTTTGATTTATATAATAAGATTTGAAAATCTATCTTTTAATAAAAATAAATTTTATGCTATAATACAACAGATAACAA
TTTAAATTGGAGGAAATGAC

Downstream 100 bases:

>100_bases
TTTAAAAGTGGGATGTATCAATAGGATTTTTGATACATCCCACTTAATTTTTATAAATTTGCAAGTAATCTTTCTTATTT
AATTAGGATGTATTTTTATT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MTLENNMDKKLFCISTYGCQMNEEDSEKLSGMLKSQGYERTENKEEASIIIFNTCCVRENAENKVFGNLGQLKQLKKKNP
NLVIAICGCMMQQVGMADKVLKTFPYVDIIFGTHNAHKFPEYLHRVLQEGVQVKEILNKEEGIVEGLPIDRKSDVKAFVT
IMYGCNNFCTYCIVPYVRGRERSRKSEDIIKEIEELVSQGYKEITLLGQNVNSYGKGLEEDIDFAGLLRKVNEVKGLERV
RFMTSHPKDLSDDVIMAIKECDKLCEQVHLPVQSGSSRILKEMNRHYDREYYLDLVKKIKSEIPDVTLTTDIIIGFPGET
EEDFLDTLSLCEEVGYDSAFTFIYSRRNHTPADKMENQIPDDIKHDRFNRLVEAINKKVVIKNKEYEGKVVEVLVEGPSK
NDETKLTGRTRNGKLVNFAGDEKLVGELVNLKIVRAQPFSLIGEIVE

Sequences:

>Translated_447_residues
MTLENNMDKKLFCISTYGCQMNEEDSEKLSGMLKSQGYERTENKEEASIIIFNTCCVRENAENKVFGNLGQLKQLKKKNP
NLVIAICGCMMQQVGMADKVLKTFPYVDIIFGTHNAHKFPEYLHRVLQEGVQVKEILNKEEGIVEGLPIDRKSDVKAFVT
IMYGCNNFCTYCIVPYVRGRERSRKSEDIIKEIEELVSQGYKEITLLGQNVNSYGKGLEEDIDFAGLLRKVNEVKGLERV
RFMTSHPKDLSDDVIMAIKECDKLCEQVHLPVQSGSSRILKEMNRHYDREYYLDLVKKIKSEIPDVTLTTDIIIGFPGET
EEDFLDTLSLCEEVGYDSAFTFIYSRRNHTPADKMENQIPDDIKHDRFNRLVEAINKKVVIKNKEYEGKVVEVLVEGPSK
NDETKLTGRTRNGKLVNFAGDEKLVGELVNLKIVRAQPFSLIGEIVE
>Mature_446_residues
TLENNMDKKLFCISTYGCQMNEEDSEKLSGMLKSQGYERTENKEEASIIIFNTCCVRENAENKVFGNLGQLKQLKKKNPN
LVIAICGCMMQQVGMADKVLKTFPYVDIIFGTHNAHKFPEYLHRVLQEGVQVKEILNKEEGIVEGLPIDRKSDVKAFVTI
MYGCNNFCTYCIVPYVRGRERSRKSEDIIKEIEELVSQGYKEITLLGQNVNSYGKGLEEDIDFAGLLRKVNEVKGLERVR
FMTSHPKDLSDDVIMAIKECDKLCEQVHLPVQSGSSRILKEMNRHYDREYYLDLVKKIKSEIPDVTLTTDIIIGFPGETE
EDFLDTLSLCEEVGYDSAFTFIYSRRNHTPADKMENQIPDDIKHDRFNRLVEAINKKVVIKNKEYEGKVVEVLVEGPSKN
DETKLTGRTRNGKLVNFAGDEKLVGELVNLKIVRAQPFSLIGEIVE

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=458, Percent_Identity=40.174672489083, Blast_Score=309, Evalue=3e-84,
Organism=Homo sapiens, GI28872784, Length=458, Percent_Identity=40.174672489083, Blast_Score=309, Evalue=3e-84,
Organism=Homo sapiens, GI93277076, Length=367, Percent_Identity=32.1525885558583, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1786882, Length=443, Percent_Identity=42.2121896162528, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1787057, Length=447, Percent_Identity=26.3982102908277, Blast_Score=158, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17553146, Length=433, Percent_Identity=35.5658198614319, Blast_Score=260, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI71996771, Length=320, Percent_Identity=24.0625, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356207, Length=423, Percent_Identity=38.0614657210402, Blast_Score=293, Evalue=1e-79,
Organism=Drosophila melanogaster, GI19922432, Length=452, Percent_Identity=29.646017699115, Blast_Score=171, Evalue=8e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_CLOP1 (Q0TRE5)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_695795.1
- ProteinModelPortal:   Q0TRE5
- SMR:   Q0TRE5
- STRING:   Q0TRE5
- GeneID:   4202345
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_1349
- TIGR:   CPF_1349
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14328
- BioCyc:   CPER195103:CPF_1349-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51040; Mature: 50909

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLENNMDKKLFCISTYGCQMNEEDSEKLSGMLKSQGYERTENKEEASIIIFNTCCVREN
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCC
AENKVFGNLGQLKQLKKKNPNLVIAICGCMMQQVGMADKVLKTFPYVDIIFGTHNAHKFP
CCCCHHCCHHHHHHHHHCCCCEEEEHHHHHHHHHCHHHHHHHHCCCEEEEEECCCCHHHH
EYLHRVLQEGVQVKEILNKEEGIVEGLPIDRKSDVKAFVTIMYGCNNFCTYCIVPYVRGR
HHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ERSRKSEDIIKEIEELVSQGYKEITLLGQNVNSYGKGLEEDIDFAGLLRKVNEVKGLERV
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RFMTSHPKDLSDDVIMAIKECDKLCEQVHLPVQSGSSRILKEMNRHYDREYYLDLVKKIK
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHH
SEIPDVTLTTDIIIGFPGETEEDFLDTLSLCEEVGYDSAFTFIYSRRNHTPADKMENQIP
HHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCHHHHHHCCC
DDIKHDRFNRLVEAINKKVVIKNKEYEGKVVEVLVEGPSKNDETKLTGRTRNGKLVNFAG
CHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEEECCCCCCCCEEECCCCCCEEEEECC
DEKLVGELVNLKIVRAQPFSLIGEIVE
CHHHHHHHHCEEEEECCCHHHHHHHCC
>Mature Secondary Structure 
TLENNMDKKLFCISTYGCQMNEEDSEKLSGMLKSQGYERTENKEEASIIIFNTCCVREN
CCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCC
AENKVFGNLGQLKQLKKKNPNLVIAICGCMMQQVGMADKVLKTFPYVDIIFGTHNAHKFP
CCCCHHCCHHHHHHHHHCCCCEEEEHHHHHHHHHCHHHHHHHHCCCEEEEEECCCCHHHH
EYLHRVLQEGVQVKEILNKEEGIVEGLPIDRKSDVKAFVTIMYGCNNFCTYCIVPYVRGR
HHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
ERSRKSEDIIKEIEELVSQGYKEITLLGQNVNSYGKGLEEDIDFAGLLRKVNEVKGLERV
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RFMTSHPKDLSDDVIMAIKECDKLCEQVHLPVQSGSSRILKEMNRHYDREYYLDLVKKIK
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHH
SEIPDVTLTTDIIIGFPGETEEDFLDTLSLCEEVGYDSAFTFIYSRRNHTPADKMENQIP
HHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCHHHHHHCCC
DDIKHDRFNRLVEAINKKVVIKNKEYEGKVVEVLVEGPSKNDETKLTGRTRNGKLVNFAG
CHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEEECCCCCCCCEEECCCCCCEEEEECC
DEKLVGELVNLKIVRAQPFSLIGEIVE
CHHHHHHHHCEEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA