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The map label for this gene is pac

Identifier: 18310074

GI number: 18310074

Start: 1293432

End: 1294415

Strand: Direct

Name: pac

Synonym: CPE1092

Alternate gene names: NA

Gene position: 1293432-1294415 (Clockwise)

Preceding gene: 18310071

Following gene: 18310075

Centisome position: 42.67

GC content: 29.88

Gene sequence:

>984_bases
GTGTGTACTCATATACATATTTCAAGTGTTAAGAATAATCTTTATTGGGGAAGAACTTTAGATACATCATTTAATCCATT
TGATGTTGATTCTAAAGTAACAATTGTTCCTAGAAATTTTACTTTAAAAACTCAAAGTAAACCATGGAAAACTAAATATG
CCTTTTTAGGTATAAATTTATGTGGATCTACTCTGTTTTTTGATGGAGTTAATGAAAAGGGGCTTGCTGGAGGTCTTTTA
TTTTTAAAGGCATGTACATGGGATAAGAAAGAAAATATAGAAAAGCAAGGACTTATAGCCATTAATAGTGGAGAAATAGT
TACTTGGATTTTAAGCAATTTTGAAAGTGTTAGTGATATAAAAAATAACATATCAAAAGTTGTAGTAACATCTGATGACA
TACCATCATTAGGAGAGCTTGGAAAAGGAAATCCTATTACTGCACATTATACTTTCACAGATAAAATGGGAGAATCAGTT
GTATTAGAACCAACAAACCATGGACATTTTCGTGTTTTTGATAATACTGTAGGGGTAATGGCTAATGATCCAACATTTGA
TTGGCACATGACTAATTTGGCAAATTATATTCAAGTTCAAGGATTTAATAAGCAACAAAATAATTTAAATAGTAAAACAG
TTATTACACCAATTTCAAATGGTACAGGTATGCTTGGATTACCTGGAGATTATACTTCACCATCAAGGTTTGTACGTGTA
GCATATTTAAGAAATCTTATAGGAGACATTACTGACGAAGAAGCTCCAGCACGTTTATTTAGTATTTTAAATTCTGTTTG
GGTTCCAAAGGATGTTGAACGTTTTTATGAAGATAAAAAAGACTCAGATTTTTCAAGTTATATGTGTGCATATGACCAAA
ACTTAGGTAAGTTATATTTACGTGTTTTCAATCATATAGATACAATGGAATTCTCTTTAGAAAATGTTAAAGAAGATGAA
TTAGTAACTTATTCAGTTCAATAA

Upstream 100 bases:

>100_bases
TTAGTATACATATTATGCATTAAGCCTAAATTGACAATAAATATGTGTAGGTATACTCTAAAAATATAATTTAAAATTGT
TTTTTTAGGAGATGATTTAG

Downstream 100 bases:

>100_bases
ATAATTTTTACATAGCAATAAAAAGTCTTAGAAATAAGACTTTTTTTATTTTAAATTCAGTTTATTGTTAGAAGTAATTT
AATCTTTTTGATTTATATAA

Product: choloylglycine hydrolase family protein

Products: NA

Alternate protein names: Penicillin V amidase; PVA [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MCTHIHISSVKNNLYWGRTLDTSFNPFDVDSKVTIVPRNFTLKTQSKPWKTKYAFLGINLCGSTLFFDGVNEKGLAGGLL
FLKACTWDKKENIEKQGLIAINSGEIVTWILSNFESVSDIKNNISKVVVTSDDIPSLGELGKGNPITAHYTFTDKMGESV
VLEPTNHGHFRVFDNTVGVMANDPTFDWHMTNLANYIQVQGFNKQQNNLNSKTVITPISNGTGMLGLPGDYTSPSRFVRV
AYLRNLIGDITDEEAPARLFSILNSVWVPKDVERFYEDKKDSDFSSYMCAYDQNLGKLYLRVFNHIDTMEFSLENVKEDE
LVTYSVQ

Sequences:

>Translated_327_residues
MCTHIHISSVKNNLYWGRTLDTSFNPFDVDSKVTIVPRNFTLKTQSKPWKTKYAFLGINLCGSTLFFDGVNEKGLAGGLL
FLKACTWDKKENIEKQGLIAINSGEIVTWILSNFESVSDIKNNISKVVVTSDDIPSLGELGKGNPITAHYTFTDKMGESV
VLEPTNHGHFRVFDNTVGVMANDPTFDWHMTNLANYIQVQGFNKQQNNLNSKTVITPISNGTGMLGLPGDYTSPSRFVRV
AYLRNLIGDITDEEAPARLFSILNSVWVPKDVERFYEDKKDSDFSSYMCAYDQNLGKLYLRVFNHIDTMEFSLENVKEDE
LVTYSVQ
>Mature_327_residues
MCTHIHISSVKNNLYWGRTLDTSFNPFDVDSKVTIVPRNFTLKTQSKPWKTKYAFLGINLCGSTLFFDGVNEKGLAGGLL
FLKACTWDKKENIEKQGLIAINSGEIVTWILSNFESVSDIKNNISKVVVTSDDIPSLGELGKGNPITAHYTFTDKMGESV
VLEPTNHGHFRVFDNTVGVMANDPTFDWHMTNLANYIQVQGFNKQQNNLNSKTVITPISNGTGMLGLPGDYTSPSRFVRV
AYLRNLIGDITDEEAPARLFSILNSVWVPKDVERFYEDKKDSDFSSYMCAYDQNLGKLYLRVFNHIDTMEFSLENVKEDE
LVTYSVQ

Specific function: The enzyme catalyzes the conversion of penicillin to 6- aminopenicillanate The precursor, furthermore, acts as a self- processing peptidase that cleaves off the propeptide. All peptidase activity is lost on conversion to the mature peptidase [H]

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: =3.5.1.11 [H]

Molecular weight: Translated: 36863; Mature: 36863

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTHIHISSVKNNLYWGRTLDTSFNPFDVDSKVTIVPRNFTLKTQSKPWKTKYAFLGINL
CCEEEEHHHHCCCEEECEEECCCCCCCCCCCEEEEEECCEEEEECCCCCCEEEEEEEEEE
CGSTLFFDGVNEKGLAGGLLFLKACTWDKKENIEKQGLIAINSGEIVTWILSNFESVSDI
CCCEEEECCCCCCCCCCCEEEEEEECCCHHHCCCCCCEEEECCCCEEEEEHHCCHHHHHH
KNNISKVVVTSDDIPSLGELGKGNPITAHYTFTDKMGESVVLEPTNHGHFRVFDNTVGVM
HHCCEEEEEECCCCCCHHHCCCCCCEEEEEEEHHCCCCEEEEECCCCCCEEEECCCEEEE
ANDPTFDWHMTNLANYIQVQGFNKQQNNLNSKTVITPISNGTGMLGLPGDYTSPSRFVRV
ECCCCCEEEHHHHHCEEEEECCCCHHCCCCCCEEEEECCCCCEEEECCCCCCCHHHHHHH
AYLRNLIGDITDEEAPARLFSILNSVWVPKDVERFYEDKKDSDFSSYMCAYDQNLGKLYL
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHH
RVFNHIDTMEFSLENVKEDELVTYSVQ
HHHHHHCHHHHHHCCCCCCCEEEEEEC
>Mature Secondary Structure
MCTHIHISSVKNNLYWGRTLDTSFNPFDVDSKVTIVPRNFTLKTQSKPWKTKYAFLGINL
CCEEEEHHHHCCCEEECEEECCCCCCCCCCCEEEEEECCEEEEECCCCCCEEEEEEEEEE
CGSTLFFDGVNEKGLAGGLLFLKACTWDKKENIEKQGLIAINSGEIVTWILSNFESVSDI
CCCEEEECCCCCCCCCCCEEEEEEECCCHHHCCCCCCEEEECCCCEEEEEHHCCHHHHHH
KNNISKVVVTSDDIPSLGELGKGNPITAHYTFTDKMGESVVLEPTNHGHFRVFDNTVGVM
HHCCEEEEEECCCCCCHHHCCCCCCEEEEEEEHHCCCCEEEEECCCCCCEEEECCCEEEE
ANDPTFDWHMTNLANYIQVQGFNKQQNNLNSKTVITPISNGTGMLGLPGDYTSPSRFVRV
ECCCCCEEEHHHHHCEEEEECCCCHHCCCCCCEEEEECCCCCEEEECCCCCCCHHHHHHH
AYLRNLIGDITDEEAPARLFSILNSVWVPKDVERFYEDKKDSDFSSYMCAYDQNLGKLYL
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHH
RVFNHIDTMEFSLENVKEDELVTYSVQ
HHHHHHCHHHHHHCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3026906 [H]