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The map label for this gene is rffG [H]

Identifier: 182680402

GI number: 182680402

Start: 3979415

End: 3980506

Strand: Reverse

Name: rffG [H]

Synonym: Bind_3502

Alternate gene names: 182680402

Gene position: 3980506-3979415 (Counterclockwise)

Preceding gene: 182680403

Following gene: 182680401

Centisome position: 95.45

GC content: 57.88

Gene sequence:

>1092_bases
ATGATGGCGCGTTTTCTCGTGACCGGCGGTGCCGGCTTCATTGGCTCGGCCGTGGTCCGTCACCTGATCGAGGATACGCC
GCACGAGGTTTGCGTCCTCGACAAGCTCACTTATGCTGGCAATCTCGATAATCTCGAACCGGTTGCCAAAAATCCGCGCT
ATCGTTTCAAGCAGGTGGATATTTGTGACCGGGCTGCGGTCGCGACACTCATAGCCTCCTACCAGCCCCGGATCATCATG
CATCTGGCGGCGGAAAGCCATGTCGATCGCTCAATCGATGGGCCAGCGGCCTTTATCGAAACCAATCTCGTCGGCACATT
CACTTTGCTCGAGGCGGCGCGTTGTTTCTGGCAAGACCTGCCGGGTGAAGAGAAAGCCGCTTTCCGCTTCCATCATGTCT
CGACCGATGAAGTGTTTGGATCCCTCGGCGCGGACGGGTTTTTCCAAGAGACCACCGCTTATCAGCCGAACTCGCCCTAT
TCCGCCTCGAAAGCCGGCTCTGATCATCTGGTGCGCGCCTGGGGCCATACTTATGGCCTGCCGACTGTGACCTCCAATTG
TTCGAATAATTACGGGCCCTATCATTTCCCCGAAAAACTGATTCCCCTGATGATCCTGAATGCGCTGGAGGGAAAGCCCC
TGCCCGTCTACGGCAATGGCCAGAACATCCGTGATTGGCTCTATGTCGAGGATCATGCCCGCGCGCTCGTCGCCATAGCG
CTCGCGGGCAGGCCGGGTGAAAGTTATAATGTCGGTGGCTGGAATGAGCGTGCCAATATTGATGTCGTGCAGGCGGTCTG
CGCACTCGTTGATGAAATGGCGCCCGATGCAGCGATCGGTCCGCGTGAGAAACTCATCACTTTCGTCACCGACCGGCCGG
GGCATGACGCACGCTATGCGATCGATGCCACCAAGATCAAACAGGATCTCGGCTGGACGCCGCGCGAAACATTCGAGACG
GGCTTGCGGAAAACGGTGCGCTGGTATCTCGATCATCCAGAATGGTGGCAACGCATACGCTCAGGCCTTTATCGCGGCGA
AAGACTCGGCGCTCTTGGTGGTGAGGGAGGCGCTATTGGCAAAGTCGCCTGA

Upstream 100 bases:

>100_bases
CCTGGCCGCTCGATGGGAAAGCGGCGATGATCTCGGATAAGGACGGCAAGCTCCCCTTCCTGCGTGATTTCAAGTCTCCC
TTCACTTTCGAGGTGCCGGC

Downstream 100 bases:

>100_bases
TGCTCCGATCCTGGTCTTTGGCGCCGGCGGCCAATTGGGCCAGGAGGTTATGGCCCAAGGGTCTGCGCGCGGCCTGCCCG
TTCAGGGTGTGAAGCGCGCC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MMARFLVTGGAGFIGSAVVRHLIEDTPHEVCVLDKLTYAGNLDNLEPVAKNPRYRFKQVDICDRAAVATLIASYQPRIIM
HLAAESHVDRSIDGPAAFIETNLVGTFTLLEAARCFWQDLPGEEKAAFRFHHVSTDEVFGSLGADGFFQETTAYQPNSPY
SASKAGSDHLVRAWGHTYGLPTVTSNCSNNYGPYHFPEKLIPLMILNALEGKPLPVYGNGQNIRDWLYVEDHARALVAIA
LAGRPGESYNVGGWNERANIDVVQAVCALVDEMAPDAAIGPREKLITFVTDRPGHDARYAIDATKIKQDLGWTPRETFET
GLRKTVRWYLDHPEWWQRIRSGLYRGERLGALGGEGGAIGKVA

Sequences:

>Translated_363_residues
MMARFLVTGGAGFIGSAVVRHLIEDTPHEVCVLDKLTYAGNLDNLEPVAKNPRYRFKQVDICDRAAVATLIASYQPRIIM
HLAAESHVDRSIDGPAAFIETNLVGTFTLLEAARCFWQDLPGEEKAAFRFHHVSTDEVFGSLGADGFFQETTAYQPNSPY
SASKAGSDHLVRAWGHTYGLPTVTSNCSNNYGPYHFPEKLIPLMILNALEGKPLPVYGNGQNIRDWLYVEDHARALVAIA
LAGRPGESYNVGGWNERANIDVVQAVCALVDEMAPDAAIGPREKLITFVTDRPGHDARYAIDATKIKQDLGWTPRETFET
GLRKTVRWYLDHPEWWQRIRSGLYRGERLGALGGEGGAIGKVA
>Mature_363_residues
MMARFLVTGGAGFIGSAVVRHLIEDTPHEVCVLDKLTYAGNLDNLEPVAKNPRYRFKQVDICDRAAVATLIASYQPRIIM
HLAAESHVDRSIDGPAAFIETNLVGTFTLLEAARCFWQDLPGEEKAAFRFHHVSTDEVFGSLGADGFFQETTAYQPNSPY
SASKAGSDHLVRAWGHTYGLPTVTSNCSNNYGPYHFPEKLIPLMILNALEGKPLPVYGNGQNIRDWLYVEDHARALVAIA
LAGRPGESYNVGGWNERANIDVVQAVCALVDEMAPDAAIGPREKLITFVTDRPGHDARYAIDATKIKQDLGWTPRETFET
GLRKTVRWYLDHPEWWQRIRSGLYRGERLGALGGEGGAIGKVA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=341, Percent_Identity=35.1906158357771, Blast_Score=224, Evalue=6e-59,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=25.1479289940828, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI4504031, Length=345, Percent_Identity=25.5072463768116, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI48994969, Length=355, Percent_Identity=66.1971830985916, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI1788353, Length=352, Percent_Identity=62.2159090909091, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1788366, Length=350, Percent_Identity=25.4285714285714, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786974, Length=360, Percent_Identity=25.5555555555556, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=331, Percent_Identity=34.1389728096677, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI115532424, Length=326, Percent_Identity=33.1288343558282, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17539532, Length=332, Percent_Identity=24.0963855421687, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=345, Percent_Identity=23.1884057971014, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=243, Percent_Identity=25.1028806584362, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=243, Percent_Identity=25.1028806584362, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=243, Percent_Identity=25.1028806584362, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=243, Percent_Identity=25.1028806584362, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=243, Percent_Identity=25.1028806584362, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=366, Percent_Identity=24.3169398907104, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21356223, Length=336, Percent_Identity=26.1904761904762, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19923002, Length=362, Percent_Identity=25.9668508287293, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24158427, Length=334, Percent_Identity=25.4491017964072, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40068; Mature: 40068

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMARFLVTGGAGFIGSAVVRHLIEDTPHEVCVLDKLTYAGNLDNLEPVAKNPRYRFKQVD
CCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCHHHCCCCCEEEECC
ICDRAAVATLIASYQPRIIMHLAAESHVDRSIDGPAAFIETNLVGTFTLLEAARCFWQDL
HHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHC
PGEEKAAFRFHHVSTDEVFGSLGADGFFQETTAYQPNSPYSASKAGSDHLVRAWGHTYGL
CCCCCCEEEEEECCHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
PTVTSNCSNNYGPYHFPEKLIPLMILNALEGKPLPVYGNGQNIRDWLYVEDHARALVAIA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEECHHHCEEEEEE
LAGRPGESYNVGGWNERANIDVVQAVCALVDEMAPDAAIGPREKLITFVTDRPGHDARYA
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEECCCCCCCEEE
IDATKIKQDLGWTPRETFETGLRKTVRWYLDHPEWWQRIRSGLYRGERLGALGGEGGAIG
EHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KVA
CCC
>Mature Secondary Structure
MMARFLVTGGAGFIGSAVVRHLIEDTPHEVCVLDKLTYAGNLDNLEPVAKNPRYRFKQVD
CCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCHHHCCCCCEEEECC
ICDRAAVATLIASYQPRIIMHLAAESHVDRSIDGPAAFIETNLVGTFTLLEAARCFWQDL
HHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHC
PGEEKAAFRFHHVSTDEVFGSLGADGFFQETTAYQPNSPYSASKAGSDHLVRAWGHTYGL
CCCCCCEEEEEECCHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
PTVTSNCSNNYGPYHFPEKLIPLMILNALEGKPLPVYGNGQNIRDWLYVEDHARALVAIA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEECHHHCEEEEEE
LAGRPGESYNVGGWNERANIDVVQAVCALVDEMAPDAAIGPREKLITFVTDRPGHDARYA
EECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEECCCCCCCEEE
IDATKIKQDLGWTPRETFETGLRKTVRWYLDHPEWWQRIRSGLYRGERLGALGGEGGAIG
EHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KVA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]